Reviewed, UniProtKB/Swiss-Prot Q9TV70 (DHDH_RABIT)

Names and origin

Protein names

Recommended name:
    Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
    EC=1.3.1.20
Alternative name(s):
    Dimeric dihydrodiol dehydrogenase
    D-xylose-NADP dehydrogenase
    EC=1.1.1.179
    D-xylose 1-dehydrogenase
    ORY2DD

Gene names

Name:	DHDH
Synonyms:	2DD

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	329 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH. D-xylose + NADP+ = D-xylono-1,5-lactone + NADPH.
Enzyme regulation	Stimulated by various salts. Ref.2
Subunit structure	Homodimer. Ref.1
Tissue specificity	Lens, liver and small intestine. Ref.1
Sequence similarities	Belongs to the gfo/idh/mocA family.
biophysicochemical properties	Kinetic parameters: KM=4.4 mM for D-xylose Vmax=3.6 µmol/min/mg enzyme with D-xylose as substrate

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-xylose 1-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 329	›329	Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase		PRO_0000315367
Sites
	Site	65	1	May play an important role in coenzyme binding By similarity
	Site	73	1	May play an important role in coenzyme binding By similarity
	Site	91	1	May play an important role in coenzyme binding By similarity
	Site	170	1	May play an important role for the adaptation of the alcohol substrate into the binding site By similarity
	Site	174	1	May play an important role in catalytic activity By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9TV70-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DD61E87AC0BAD4E2	FASTA	329	35,795
	10 20 30 40 50 60 VVSAGLIAGD FVTVLQALPR SEHQVVAVAA RDLRRAEEFA RTHGIPKAYG SYEELAKDPD 70 80 90 100 110 120 VEVAYIGTQH PQHKAAVLLF LAAGKAVLCE KPLGVNAAEV REMVAEARSR GLFLMEAIWT 130 140 150 160 170 180 RCFPAVDALK SLLAQGALGD LRVARAEFGE NLTQVLRSVD WAQAGGGLLD LGIYCVQFIS 190 200 210 220 230 240 MVFGGQKPEK ISAVGRRYET GVDDTVTVLL QYPGGVHGSF TCSISSKLSN TCSVSGTKGI 250 260 270 280 290 300 AQLLEPCWCP TELVVNKERK EFPLAPEENK KFNYRNGMGM SYEAQHVRDC LRKGLKESPV 310 320 IPLAESQLLA DILEEVRKAI GVTFPQDKH

References

[1]	"Cloning and sequencing of the cDNA species for mammalian dimeric dihydrodiol dehydrogenases." Arimitsu E., Aoki S., Ishikura S., Nakanishi K., Matsuura K., Hara A. Biochem. J. 342:721-728(1999) [PubMed: 10477285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, TISSUE SPECIFICITY. Tissue: Lens.
[2]	"Identity of dimeric dihydrodiol dehydrogenase as NADP(+)-dependent D-xylose dehydrogenase in pig liver." Aoki S., Ishikura S., Asada Y., Usami N., Hara A. Chem. Biol. Interact. 130:775-784(2001) [PubMed: 11306093] [Abstract] Cited for: ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Lens.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB021928 mRNA. Translation: BAA83485.1.
UniGene	Ocu.6266
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000006419. Oryctolagus cuniculus. [Contig view]
Phylogenomic databases
HOVERGEN	Q9TV70.
Enzyme and pathway databases
BRENDA	1.1.1.179. 255. 1.3.1.20. 255.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000683. Oxidoreductase_N. IPR004104. OxRdtase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01408. GFO_IDH_MocA. 1 hit. PF02894. GFO_IDH_MocA_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHDH_RABIT

Accession

Primary (citable) accession number: Q9TV70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)