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Reviewed, UniProtKB/Swiss-Prot Q9TV64 (AK1D1_RABIT)

Last modified June 16, 2009. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    3-oxo-5-beta-steroid 4-dehydrogenase
    EC=1.3.1.3
Alternative name(s):
    Delta(4)-3-ketosteroid 5-beta-reductase
    Delta(4)-3-oxosteroid 5-beta-reductase
    Aldo-keto reductase family 1 member D1
Gene names
Name: AKR1D1
OrganismOryctolagus cuniculus (Rabbit)
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

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Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.

Catalytic activity

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.

17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the aldo/keto reductase family.

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Ontologies

Keywords
   Biological processBile acid catabolism
Lipid metabolism
Steroid metabolism
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processbile acid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondelta4-3-oxosteroid 5beta-reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3263263-oxo-5-beta-steroid 4-dehydrogenase
PRO_0000124671

Regions

Nucleotide binding169 – 1702NADP By similarity
Nucleotide binding220 – 2245NADP By similarity
Nucleotide binding273 – 28311NADP By similarity

Sites

Active site581Proton donor By similarity
Binding site531NADP By similarity
Binding site1931NADP By similarity
Binding site2301Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9TV64-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: DA7618FFC3F849AC

FASTA32637,491
        10         20         30         40         50         60 
MDLSATNHRI PLGDGNSIPI IGLGTYSEPK TTPKGSCATS VKIAIDTGYR HIDGAYIYQN 

        70         80         90        100        110        120 
EHEVGETFRE KIAEGKVRRE DIFYCGKLWA TNHDPVMVRP TLERTLKVLK LDYIDLYIIE 

       130        140        150        160        170        180 
IPMAFKPGDV VYPRDENGKW LYHKTNLCAT WEALEACKDA GLVKSLGVSN FNRQQLELLL 

       190        200        210        220        230        240 
NKPGLKHKPV CNQVECHPYF TQPKLLKFCQ QHDIIIVAYS PLGTCRNPMW VNTSLPPLLK 

       250        260        270        280        290        300 
DTLLNSLGKK YKKTAAQIVL RFNVQRGVVV IPKSFNPERI KENFQIFDFS LTEEEMKDIE 

       310        320 
ALNKNVRYVE LLMWRDHPEY PFNDEY

« Hide

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References

[1]"Delta4-3-oxosteroid 5beta-reductase."
Yamada M., Setoguchi Y., Setoguchi T.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
Tissue: Liver.

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Cross-references

Sequence databases

AB023951 mRNA. Translation: BAA82867.1.
RefSeqNP_001075466.1.
UniGeneOcu.2365

3D structure databases

HSSPHSSP built from PDB template 1J96 based on UniProtKB P52895.
ModBaseSearch...

Genome annotation databases

GeneID100008612.

Phylogenomic databases

HOVERGENQ9TV64.

Enzyme and pathway databases

BRENDA1.3.1.3. 255.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
ProDomPD000288. Aldo/ket_red. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAK1D1_RABIT
AccessionPrimary (citable) accession number: Q9TV64
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 1, 2000
Last modified: June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents