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Q9TV52 (AT12A_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium-transporting ATPase alpha chain 2
EC=3.6.3.10
Alternative name(s):
HK alpha 2
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene names
Name:ATP12A
Synonyms:ATP1AL1
OrganismOryctolagus cuniculus (Rabbit) [Complete proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length1094 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activity

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Subunit structure

Composed of two subunits: alpha (catalytic) and beta.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Found in the skin, kidney, distal colon and brain. In the kidney it is found in the connecting tubule, cortical collecting duct and outer medullary collecting duct while in the brain it is specific to choroid plexus and cortex. Ref.3

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Potassium transport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrogen:potassium-exchanging ATPase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9TV52-1)

Also known as: 2c;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9TV52-2)

Also known as: 2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: MAGGAHRADRATGEERKEGGGRWRAPHSPSPPGPRGCPVPLKAAAQSLCRKPTWGRYCTLLLF → MR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10941094Potassium-transporting ATPase alpha chain 2
PRO_0000046262

Regions

Topological domain56 – 157102Cytoplasmic Potential
Transmembrane158 – 17821Helical; Potential
Topological domain179 – 20123Lumenal Potential
Transmembrane202 – 22221Helical; Potential
Topological domain223 – 358136Cytoplasmic Potential
Transmembrane359 – 37820Helical; Potential
Topological domain379 – 39012Lumenal Potential
Transmembrane391 – 40818Helical; Potential
Topological domain409 – 842434Cytoplasmic Potential
Transmembrane843 – 86220Helical; Potential
Topological domain863 – 87210Lumenal Potential
Transmembrane873 – 89321Helical; Potential
Topological domain894 – 91320Cytoplasmic Potential
Transmembrane914 – 93623Helical; Potential
Topological domain937 – 98852Lumenal Potential
Transmembrane989 – 100820Helical; Potential
Topological domain1009 – 102214Cytoplasmic Potential
Transmembrane1023 – 104119Helical; Potential
Topological domain1042 – 105615Lumenal Potential
Transmembrane1057 – 107721Helical; Potential
Topological domain1078 – 109417Cytoplasmic Potential

Sites

Active site44614-aspartylphosphate intermediate By similarity
Metal binding7871Magnesium By similarity
Metal binding7911Magnesium By similarity

Amino acid modifications

Modified residue10131Phosphoserine; by PKA By similarity

Natural variations

Alternative sequence1 – 6363MAGGA…TLLLF → MR in isoform Short.
VSP_000413

Experimental info

Sequence conflict3001G → E in AAD11800. Ref.2
Sequence conflict4211V → M in AAD11800. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long (2c) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 2A8926D8135AE92C

FASTA1,094120,966
        10         20         30         40         50         60 
MAGGAHRADR ATGEERKEGG GRWRAPHSPS PPGPRGCPVP LKAAAQSLCR KPTWGRYCTL 

        70         80         90        100        110        120 
LLFQRKLEIY SVELHAATDI KKKEGRDGKK DNDLELKRNQ QKEELKKELD LDDHKLSNKE 

       130        140        150        160        170        180 
LETKYGTDII RGLSSTRAAE LLAQNGPNAL TPPKQTPEII KFLKQMVGGF SILLWVGAVL 

       190        200        210        220        230        240 
CWIAFGIQYV SNPSASLDRV YLGTVLAVVV ILTGIFAYYQ EAKSTNIMAS FCKMIPQQAV 

       250        260        270        280        290        300 
VIRDSEKKVI PAEQLVVGDI VEIKGGDQIP ADIRLLSAQG CKVDNSSLTG ESEPQSRSSG 

       310        320        330        340        350        360 
FTHENPLETK NITFYSTTCL EGTATGMVIN TGDRTIIGRI ASLASGVGNE KTPIAIEIEH 

       370        380        390        400        410        420 
FVHIVAGVAV SVGILFFIIA VCMKYHVLDA IIFLIAIIVA NVPEGLLATV TVALSLTAKR 

       430        440        450        460        470        480 
VAKKNCLVKN LEAVETLGST SIICSDKTGT LTQNRMTVAH LWFDNQIFVA DTSEDNLNQG 

       490        500        510        520        530        540 
FDQSSGTWTS LSKIIALCNR AEFKPGEESV PIMKRVVVGD ASETALLKFS EVILGDVMEI 

       550        560        570        580        590        600 
RKRNHKVVEI PFNSTNKFQL SIHQTEDPND KRFLLVMKGA PERILEKCST IMINGKEQPL 

       610        620        630        640        650        660 
DKSMAQAFHT AYMELGGLGE RVLGFCHFYL PADEFPETYS FDSESMNFPT SNLCFVGLLS 

       670        680        690        700        710        720 
MIDPPRSTVP DAVTKCRSAG IKVIMVTGDH PITAKAIAKS VGIISANSET VEDIAKRCNI 

       730        740        750        760        770        780 
AVEQVNKRDA KAAVVTGMEL KDMSPEQLDE LLANYPEIVF ARTSPQQKLI IVEGCQRQDA 

       790        800        810        820        830        840 
VVAVTGDGVN DSPALKKADI GVAMGITGSD AAKNAADMIL LDDNFSSIVT GVEEGRLIFD 

       850        860        870        880        890        900 
NLKKTIAYTL TKNIAELCPF LIYIILGLPL PIGTITLLFI DLGTDIIPSI ALAYEKAESD 

       910        920        930        940        950        960 
IMNRKPRHKK KDRLVNQQLA VYSYLHIGLM QALGAFLVYF TVYAQQGFRP TSLFHLRIAW 

       970        980        990       1000       1010       1020 
DSDHLNDLED NYGQEWTSYQ RQYLEWTGYT AFFVGIMVQQ IADLIIRKTR KNSIFKQGLF 

      1030       1040       1050       1060       1070       1080 
RNKVIWVGIA SQIIVALLLS YGLGSITALN FTMLKAQYWF VAVPHAILIW VYDEMRKLFI 

      1090 
RLYPGSWWDK NMYY

« Hide

Isoform Short (2a) [UniParc].

Checksum: F0E79E4159119DFF
Show »

FASTA1,033114,503

References

[1]"H-K-ATPase in the RCCT-28A rabbit cortical collecting duct cell line."
Campbell W.G., Weiner I.D., Wingo C.S., Cain B.D.
Am. J. Physiol. 276:F237-F245(1999) [PubMed: 9950954] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Strain: New Zealand white.
Tissue: Kidney cortex.
[2]"Intrarenal distribution of the colonic H,K-ATPase mRNA in rabbit."
Fejes-Toth G., Naray-Fejes-Toth A., Velazquez H.
Kidney Int. 56:1029-1036(1999) [PubMed: 10469371] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Renal collecting duct.
[3]"Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF023128 mRNA. Translation: AAB80941.1.
AF023129 mRNA. Translation: AAC13887.1.
AF106063 mRNA. Translation: AAD11800.1.
RefSeqNP_001075496.1. NM_001082027.1.
UniGeneOcu.2056.

3D structure databases

ProteinModelPortalQ9TV52.
SMRQ9TV52. Positions 97-1094.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9TV52.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100008669.

Organism-specific databases

CTD479.

Phylogenomic databases

GeneTreeENSGT00560000076866.
HOVERGENHBG004298.
OrthoDBEOG41C6VF.

Family and domain databases

InterProIPR023306. ATPase_cation_domN.
IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR005775. ATPase_P-typ_cation-ex_asu_euk.
IPR006069. ATPase_P-typ_cation-exchng_asu.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00121. NAKATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF81660. ATPase_cation_domN. 1 hit.
SSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAT12A_RABIT
AccessionPrimary (citable) accession number: Q9TV52
Secondary accession number(s): Q9TSI2, Q9TV53
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 2000
Last modified: November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents