Q9TUL9 (TIMP3_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metalloproteinase inhibitor 3

Alternative name(s):
Tissue inhibitor of metalloproteinases 3
Short name=TIMP-3

Gene names

Name:

TIMP3

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.
Subunit structure	Interacts with EFEMP1 By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Sequence similarities	Belongs to the protease inhibitor I35 (TIMP) family. [View classification] Contains 1 NTR domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor Protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to organic substance Inferred from electronic annotation. Source: Compara negative regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara
Cellular_component	basement membrane Inferred from electronic annotation. Source: Compara
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 211	188	Metalloproteinase inhibitor 3		PRO_0000034340
Regions
	Domain	24 – 143	120	NTR
	Region	24 – 28	5	Involved in metalloproteinase-binding By similarity
	Region	88 – 89	2	Involved in metalloproteinase-binding By similarity
	Region	105 – 188	84	Mediates interaction with EFEMP1 By similarity
Sites
	Metal binding	24	1	Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity
Amino acid modifications
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 91		By similarity
	Disulfide bond	26 ↔ 118		By similarity
	Disulfide bond	36 ↔ 143		By similarity
	Disulfide bond	145 ↔ 192		By similarity
	Disulfide bond	150 ↔ 155		By similarity
	Disulfide bond	163 ↔ 184		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9TUL9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1E9C5A6D2FC766DF	FASTA	211	24,147
	10 20 30 40 50 60 MTPWLGLVVL LGSWSLGDWG AEACTCSPSH PQDAFCNSDI VIRAKVVGKK LVKEGPFGTL 70 80 90 100 110 120 VYTIKQMKMY RGFTKMPHVQ YIHTEASESL CGLKLEVNKY QYLLTGRVYD GKMYTGLCNF 130 140 150 160 170 180 VERWDQLTLS QRKGLNYRYH LGCNCKIKSC YYLPCYVTSK NECLWTDMLS NFGYPGYQSK 190 200 210 HYACIRQKGG YCSWYRGWAP PDKSIINATD P

References

[1]	"Molecular cloning and sequencing of the cDNA encoding equine tissue inhibitor of metalloproteinase-3 (TIMP-3)." Kyaw-Tanner M.T., Mungall B.A., Pollitt C.C. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ243283 mRNA. Translation: CAB51854.1.
RefSeq	NP_001075339.1. NM_001081870.1.
UniGene	Eca.12656.
3D structure databases
ProteinModelPortal	Q9TUL9.
SMR	Q9TUL9. Positions 24-144.
ModBase	Search...
Protein-protein interaction databases
STRING	9796.ENSECAP00000015840.
Protein family/group databases
MEROPS	I35.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSECAT00000019359; ENSECAP00000015840; ENSECAG00000018314.
GeneID	100033947.
KEGG	ecb:100033947.
Organism-specific databases
CTD	7078.
Phylogenomic databases
eggNOG	NOG250837.
GeneTree	ENSGT00390000004555.
HOGENOM	HOG000285981.
HOVERGEN	HBG068749.
InParanoid	Q9TUL9.
OMA	KEGYCSW.
OrthoDB	EOG47D9H3.
Family and domain databases
InterPro	IPR001134. Netrin_domain. IPR001820. Prot_inh_TIMP. IPR008993. TIMP-like_OB-fold. IPR015612. Tissue_inhib_metalloprotease_3. [Graphical view]
PANTHER	PTHR11844. PTHR11844. 1 hit. PTHR11844:SF6. PTHR11844:SF6. 1 hit.
Pfam	PF00965. TIMP. 1 hit. [Graphical view]
SMART	SM00206. NTR. 1 hit. [Graphical view]
SUPFAM	SSF50242. TIMP_like. 1 hit.
PROSITE	PS50189. NTR. 1 hit. PS00288. TIMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIMP3_HORSE

Accession

Primary (citable) accession number: Q9TUL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program