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Protein

Fatty-acid amide hydrolase 1

Gene

FAAH

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (By similarity).By similarity

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei142 – 1421Charge relay systemBy similarity
Binding sitei191 – 1911Substrate; via carbonyl oxygenBy similarity
Active sitei217 – 2171Charge relay systemBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Active sitei241 – 2411Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
  2. fatty acid amide hydrolase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty-acid amide hydrolase 1 (EC:3.5.1.99)
Alternative name(s):
Anandamide amidohydrolase 1
Oleamide hydrolase 1
Gene namesi
Name:FAAH
Synonyms:FAAH1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein
Note: Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 403374CytoplasmicBy similarityAdd
BLAST
Intramembranei404 – 43330By similarityAdd
BLAST
Topological domaini434 – 579146CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. organelle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi217 – 2171S → A: Loss of activity. 1 Publication
Mutagenesisi218 – 2181S → A: Lowers activity by at least 98%. 1 Publication
Mutagenesisi237 – 2371D → E or N: Loss of activity. 1 Publication
Mutagenesisi241 – 2411S → A: Loss of activity. 1 Publication
Mutagenesisi249 – 2491C → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Fatty-acid amide hydrolase 1PRO_0000105266Add
BLAST

Proteomic databases

PaxDbiQ9TUI8.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004216.

Structurei

3D structure databases

ProteinModelPortaliQ9TUI8.
SMRiQ9TUI8. Positions 37-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2414Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000016500.
HOVERGENiHBG005632.
InParanoidiQ9TUI8.
KOiK15528.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR015830. Amidase_fun_type.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
PIRSFiPIRSF001221. Amidase_fungi. 1 hit.
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TUI8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVQEELWAAF SGPSGVALAC CLVAAALALR WSSRRMARGA AARARQRQQA
60 70 80 90 100
ALETMDKAAQ RFRLQNPDLD SEMLLALPLP QLVQKVRSGE LSPEAVLFSY
110 120 130 140 150
LQKAWEVNRG TNCVTTYLAD CEAQLCQAPG QGLLYGVPVS LKECFSCKGH
160 170 180 190 200
DSTLGLSRNQ GTPAECDCVV VQVLKLQGAV PFVHTNVPQS MFSYDCSNPL
210 220 230 240 250
FGQTTNPWMS SKSPGGSSGG EGALIAAGGS PLGLGTDIGG SIRFPSAFCG
260 270 280 290 300
ICGIKPTGNR ISKSGLKGSV YGQVAVQLSV GPMARDVESL ALCLRALLCE
310 320 330 340 350
DMFRLDPTVP PLPFNEEVYA SSRPLRVGYY ETDNYTMPTP AMRRALLETK
360 370 380 390 400
RSLEAAGHTL IPFLPANIPH ALEALSTGGL FSDGGKRLLQ NFEGDYVDSC
410 420 430 440 450
LGDLISILRL PKWLKGLLAF MLRPLLPRLA GFLSSLRPRS AGKLWELQHE
460 470 480 490 500
IEMYRHSVIA QWRALDLDVV LTPMLSPALD LNAPGKATGA VSYTLLYNCL
510 520 530 540 550
DFPAGVVPVT TVTAEDEAQM EHYKGYFGDI WDKVVQKAMK RSVGLPVAVQ
560 570
CVALPWQEEL CLRFMREVER LMAPGRQPS
Length:579
Mass (Da):62,857
Last modified:May 1, 2000 - v1
Checksum:i0600F2FDC9879D7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027132 mRNA. Translation: BAA86917.1.
RefSeqiNP_999079.1. NM_213914.1.
UniGeneiSsc.2698.

Genome annotation databases

GeneIDi396949.
KEGGissc:396949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027132 mRNA. Translation: BAA86917.1.
RefSeqiNP_999079.1. NM_213914.1.
UniGeneiSsc.2698.

3D structure databases

ProteinModelPortaliQ9TUI8.
SMRiQ9TUI8. Positions 37-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004216.

Proteomic databases

PaxDbiQ9TUI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396949.
KEGGissc:396949.

Organism-specific databases

CTDi2166.

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000016500.
HOVERGENiHBG005632.
InParanoidiQ9TUI8.
KOiK15528.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR015830. Amidase_fun_type.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
PIRSFiPIRSF001221. Amidase_fungi. 1 hit.
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Anandamide amidohydrolase of porcine brain: cDNA cloning, functional expression and site-directed mutagenesis."
    Goparaju S.K., Kurahashi Y., Suzuki H., Ueda N., Yamamoto S.
    Biochim. Biophys. Acta 1441:77-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF SER-217; SER-218; ASP-237; SER-241 AND CYS-249.
    Tissue: Brain.

Entry informationi

Entry nameiFAAH1_PIG
AccessioniPrimary (citable) accession number: Q9TUI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.