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Q9TUI8

- FAAH1_PIG

UniProt

Q9TUI8 - FAAH1_PIG

Protein

Fatty-acid amide hydrolase 1

Gene

FAAH

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates By similarity.By similarity

    Catalytic activityi

    Anandamide + H2O = arachidonic acid + ethanolamine.
    Oleamide + H2O = oleic acid + NH3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei142 – 1421Charge relay systemBy similarity
    Binding sitei191 – 1911Substrate; via carbonyl oxygenBy similarity
    Active sitei217 – 2171Charge relay systemBy similarity
    Binding sitei217 – 2171SubstrateBy similarity
    Active sitei241 – 2411Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
    2. fatty acid amide hydrolase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty-acid amide hydrolase 1 (EC:3.5.1.99)
    Alternative name(s):
    Anandamide amidohydrolase 1
    Oleamide hydrolase 1
    Gene namesi
    Name:FAAH
    Synonyms:FAAH1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein
    Note: Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. organelle membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi217 – 2171S → A: Loss of activity. 1 Publication
    Mutagenesisi218 – 2181S → A: Lowers activity by at least 98%. 1 Publication
    Mutagenesisi237 – 2371D → E or N: Loss of activity. 1 Publication
    Mutagenesisi241 – 2411S → A: Loss of activity. 1 Publication
    Mutagenesisi249 – 2491C → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 579579Fatty-acid amide hydrolase 1PRO_0000105266Add
    BLAST

    Proteomic databases

    PaxDbiQ9TUI8.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000004216.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9TUI8.
    SMRiQ9TUI8. Positions 37-572.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 403374CytoplasmicBy similarityAdd
    BLAST
    Topological domaini434 – 579146CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei404 – 43330By similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni238 – 2414Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the amidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0154.
    HOGENOMiHOG000016500.
    HOVERGENiHBG005632.
    KOiK15528.

    Family and domain databases

    Gene3Di3.90.1300.10. 1 hit.
    InterProiIPR000120. Amidase.
    IPR020556. Amidase_CS.
    IPR023631. Amidase_dom.
    IPR015830. Amidase_fun_type.
    [Graphical view]
    PANTHERiPTHR11895. PTHR11895. 1 hit.
    PfamiPF01425. Amidase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001221. Amidase_fungi. 1 hit.
    SUPFAMiSSF75304. SSF75304. 1 hit.
    PROSITEiPS00571. AMIDASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9TUI8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQEELWAAF SGPSGVALAC CLVAAALALR WSSRRMARGA AARARQRQQA    50
    ALETMDKAAQ RFRLQNPDLD SEMLLALPLP QLVQKVRSGE LSPEAVLFSY 100
    LQKAWEVNRG TNCVTTYLAD CEAQLCQAPG QGLLYGVPVS LKECFSCKGH 150
    DSTLGLSRNQ GTPAECDCVV VQVLKLQGAV PFVHTNVPQS MFSYDCSNPL 200
    FGQTTNPWMS SKSPGGSSGG EGALIAAGGS PLGLGTDIGG SIRFPSAFCG 250
    ICGIKPTGNR ISKSGLKGSV YGQVAVQLSV GPMARDVESL ALCLRALLCE 300
    DMFRLDPTVP PLPFNEEVYA SSRPLRVGYY ETDNYTMPTP AMRRALLETK 350
    RSLEAAGHTL IPFLPANIPH ALEALSTGGL FSDGGKRLLQ NFEGDYVDSC 400
    LGDLISILRL PKWLKGLLAF MLRPLLPRLA GFLSSLRPRS AGKLWELQHE 450
    IEMYRHSVIA QWRALDLDVV LTPMLSPALD LNAPGKATGA VSYTLLYNCL 500
    DFPAGVVPVT TVTAEDEAQM EHYKGYFGDI WDKVVQKAMK RSVGLPVAVQ 550
    CVALPWQEEL CLRFMREVER LMAPGRQPS 579
    Length:579
    Mass (Da):62,857
    Last modified:May 1, 2000 - v1
    Checksum:i0600F2FDC9879D7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB027132 mRNA. Translation: BAA86917.1.
    RefSeqiNP_999079.1. NM_213914.1.
    UniGeneiSsc.2698.

    Genome annotation databases

    GeneIDi396949.
    KEGGissc:396949.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB027132 mRNA. Translation: BAA86917.1 .
    RefSeqi NP_999079.1. NM_213914.1.
    UniGenei Ssc.2698.

    3D structure databases

    ProteinModelPortali Q9TUI8.
    SMRi Q9TUI8. Positions 37-572.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000004216.

    Proteomic databases

    PaxDbi Q9TUI8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396949.
    KEGGi ssc:396949.

    Organism-specific databases

    CTDi 2166.

    Phylogenomic databases

    eggNOGi COG0154.
    HOGENOMi HOG000016500.
    HOVERGENi HBG005632.
    KOi K15528.

    Family and domain databases

    Gene3Di 3.90.1300.10. 1 hit.
    InterProi IPR000120. Amidase.
    IPR020556. Amidase_CS.
    IPR023631. Amidase_dom.
    IPR015830. Amidase_fun_type.
    [Graphical view ]
    PANTHERi PTHR11895. PTHR11895. 1 hit.
    Pfami PF01425. Amidase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001221. Amidase_fungi. 1 hit.
    SUPFAMi SSF75304. SSF75304. 1 hit.
    PROSITEi PS00571. AMIDASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Anandamide amidohydrolase of porcine brain: cDNA cloning, functional expression and site-directed mutagenesis."
      Goparaju S.K., Kurahashi Y., Suzuki H., Ueda N., Yamamoto S.
      Biochim. Biophys. Acta 1441:77-84(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF SER-217; SER-218; ASP-237; SER-241 AND CYS-249.
      Tissue: Brain.

    Entry informationi

    Entry nameiFAAH1_PIG
    AccessioniPrimary (citable) accession number: Q9TUI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3