Reviewed, UniProtKB/Swiss-Prot Q9TTK8 (KCRU_BOVIN)

Names and origin

Protein names

Recommended name:
    Creatine kinase U-type, mitochondrial
    EC=2.7.3.2
Alternative name(s):
    Ubiquitous mitochondrial creatine kinase
      Short name=U-MtCK
    Acidic-type mitochondrial creatine kinase
      Short name=Mia-CK

Gene names

Name:

CKMT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four MTCK homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin By similarity.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 416	377	Creatine kinase U-type, mitochondrial		PRO_0000016589
Regions
	Nucleotide binding	161 – 165	5	ATP By similarity
	Nucleotide binding	353 – 358	6	ATP By similarity
	Region	40 – 64	25	Cardiolipin-binding By similarity
Sites
	Binding site	224	1	ATP By similarity
	Binding site	269	1	ATP By similarity
	Binding site	325	1	ATP By similarity
	Binding site	368	1	ATP By similarity
Amino acid modifications
	Modified residue	153	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9TTK8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 283F2C9F49C9C176	FASTA	416	46,897
	10 20 30 40 50 60 MAGPFSRLLS ARPGLRLLAL AGAGSLAAGF LLRSEPVRAA SERRRLYPPS AEYPDLRKHN 70 80 90 100 110 120 NCMASHLTPA VYARLCDKTT PTGWTLDQCI QTGVDNPGHP FIKTVGMVAG DEETYEVFAE 130 140 150 160 170 180 LFDPVIQERH NGYDPRTMKH TTDLDASKIR SGYFDERYVL SSRVRTGRSI RGLSLPPACT 190 200 210 220 230 240 RAERREVERV VVDALSGLKG DLAGRYYRLS EMTEAEQQQL IDDHFLFDKP VSPLLTAAGM 250 260 270 280 290 300 ARDWPDARGI WHNNEKSFLI WVNEEDHTRV ISMEKGGNMK KVFERFCRGL KEVERLIQER 310 320 330 340 350 360 GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL QKRGTGGVDT 370 380 390 400 410 AATGSVFDIS NLDRLGKSEV ELVQLVIDGV NFLIDCERRL ERGQDIRIPP PLPNKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Initiating ocular proteomics for cataloging bovine retinal proteins: microanalytical techniques permit the identification of proteins derived from a novel photoreceptor preparation." Komori N., Usukura J., Jackson K.W., Tobin S.L., Matsumoto H., Nishizawa Y. Exp. Eye Res. 69:195-212(1999) [PubMed: 10433856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB003307 mRNA. Translation: BAA88431.1. BC102422 mRNA. Translation: AAI02423.1.
IPI	IPI00692034.
RefSeq	NP_776700.1.
UniGene	Bt.49713
3D structure databases
SMR	Q9TTK8. Positions 39-412.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9TTK8.
Genome annotation databases
Ensembl	ENSBTAT00000009871; ENSBTAP00000009871; ENSBTAG00000007502; Bos taurus. [Genome view]
GeneID	281692.
KEGG	bta:281692.
Organism-specific databases
CTD	281692.
Phylogenomic databases
eggNOG	maNOG10765.
HOVERGEN	Q9TTK8.
InParanoid	Q9TTK8.
PhylomeDB	Q9TTK8.
Enzyme and pathway databases
BRENDA	2.7.3.2. 251.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRU_BOVIN

Accession

Primary (citable) accession number: Q9TTK8
Secondary accession number(s): Q3ZCF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)