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Reviewed, UniProtKB/Swiss-Prot Q9TTK6 (AOC3_BOVIN)

Last modified November 3, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Membrane primary amine oxidase
    EC=1.4.3.21
Alternative name(s):
    Copper amine oxidase
    Semicarbazide-sensitive amine oxidase
      Short name=SSAO
    Vascular adhesion protein 1
      Short name=VAP-1
Gene names
Name: AOC3
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity By similarity.

Catalytic activity

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.

Cofactor

Binds 1 copper ion per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Contains 1 topaquinone per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked. Forms heterodimer with AOC2 By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Post-translational modification

Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.

N- and O-glycosylated By similarity.

Sequence similarities

Belongs to the copper/topaquinone oxidase family.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9TTK6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9TTK6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     734-763: CEVNSLACLPKDPACAPDLPAFSHGGFFTN → SMLPESGASQ...TPRCIFRRHG
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 763762Membrane primary amine oxidase
PRO_0000064101

Regions

Topological domain2 – 65Cytoplasmic Potential
Transmembrane7 – 2721Signal-anchor for type II membrane protein Potential
Topological domain28 – 763736Extracellular Potential

Sites

Active site3861Proton acceptor By similarity
Active site4711Schiff-base intermediate with substrate; via topaquinone By similarity
Metal binding5201Copper By similarity
Metal binding5221Copper By similarity
Metal binding5291Calcium 1 By similarity
Metal binding5301Calcium 1; via carbonyl oxygen By similarity
Metal binding5311Calcium 1 By similarity
Metal binding5721Calcium 2 By similarity
Metal binding6381Calcium 2 By similarity
Metal binding6631Calcium 2; via carbonyl oxygen By similarity
Metal binding6651Calcium 2 By similarity
Metal binding6671Calcium 2 By similarity
Metal binding6731Calcium 1 By similarity
Metal binding6741Calcium 1; via carbonyl oxygen By similarity
Metal binding6841Copper By similarity

Amino acid modifications

Modified residue47112',4',5'-topaquinone By similarity
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation2121O-linked (GalNAc...) By similarity
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation6661N-linked (GlcNAc...) Potential
Disulfide bond198 ↔ 199 By similarity
Disulfide bond734 ↔ 741 By similarity
Disulfide bond748Interchain By similarity

Natural variations

Alternative sequence734 – 76330CEVNS…GFFTN → SMLPESGASQLCAPQLAGFL SQRRGTVSQEAGVMVTRFYK TPRCIFRRHG in isoform 2.
VSP_016603

Experimental info

Sequence conflict1781D → H in AAX46549. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: DB61ED9A89E71E90

FASTA76384,500
        10         20         30         40         50         60 
MNQKTTLVLL ALAVITIFAL VCVLIAGRGG DGGEASQPHY CPSGTPSVQP WTHPGQNQLF 

        70         80         90        100        110        120 
ADLSREELTA VMSFLTQKLG PDLVDAAQAR PSDNCIFSVE LQLPPKAAAL AHLDRRSPPP 

       130        140        150        160        170        180 
AREALAIVFF GGQPQPNVTE LVVGPLPQPS YMRDVTVERH GGPLPYYRRP VLLREYLDID 

       190        200        210        220        230        240 
QMIFNRELPQ AAGVLHHCCS YKQGGGNLVT MTTAPRGLQS GDRATWFGLY YNISGAGYYL 

       250        260        270        280        290        300 
HPVGLELLVD HKALDPAQWT IQKVFFQGRY YESLAQLEEQ FEAGRVNVVV IPNNGTGGSW 

       310        320        330        340        350        360 
SLKSQVPPGP TPPLQFHPQG TRFSVQGSRV TSSLWTFSFG LGAFSGPRIF DIRFQGERLA 

       370        380        390        400        410        420 
YEISLQEAVA IYGGNTPAAM LTRYMDGCFG MGKFATPLTR GVDCPYLATY VDWHFLLESQ 

       430        440        450        460        470        480 
APRTLHDAFC VFEQNKGLPL RRHHSDFISQ YFGGVVETVL VFRSVSTLLN YDYVWDMVFH 

       490        500        510        520        530        540 
PNGAIEVKFH ATGYISSAFF FGTAQKYGNQ VRENTLGTVH THSAHYKVDL DVGGLENWVW 

       550        560        570        580        590        600 
AEDMAFVPTT VPWSPEHQIQ RLQVTRKQLE TEEQAAFPLG GASPRYLYLA SKQSNKWGHP 

       610        620        630        640        650        660 
RGYRIQTVSF AGRPLPQNSS TERAISWGRY QLAVTQRKET EPSSSSVFNQ NDPWTPTVDF 

       670        680        690        700        710        720 
ADFINNETIA GKDLVAWVTA GFLHIPHAED IPNTVTVGNG VGFFLRPYNF FDEDPSINSA 

       730        740        750        760 
DSIYFQKHQD AGSCEVNSLA CLPKDPACAP DLPAFSHGGF FTN

« Hide

Isoform 2.

Checksum: 5E213F41CD04EA7C
Show »

FASTA78386,905

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References

« Hide 'large scale' references
[1]"Molecular cloning of semicarbazide-sensitive amine oxidase gene from Bovine aorta."
Iwabuki H., Matsumura K., Mure M., Kuroda S., Tanizawa K.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Hereford.
Tissue: Fetal liver.

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Cross-references

Sequence databases

AB019242 mRNA. Translation: BAA88896.1.
BT021702 mRNA. Translation: AAX46549.1.
BC140657 mRNA. Translation: AAI40658.1.
IPIIPI00700497.
IPI00715952.
RefSeqNP_851345.1.
UniGeneBt.4177
Bt.88366

3D structure databases

SMRQ9TTK6. Positions 57-717, 58-718.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9TTK6.

Genome annotation databases

EnsemblENSBTAT00000012286; ENSBTAP00000012286; ENSBTAG00000030328; Bos taurus. [Genome view]
ENSBTAT00000042826; ENSBTAP00000040440; ENSBTAG00000030328; Bos taurus. [Genome view]
GeneID281002.
KEGGbta:281002.

Organism-specific databases

CTD281002.

Phylogenomic databases

HOVERGENQ9TTK6.

Enzyme and pathway databases

BRENDA1.4.3.6. 251.

Family and domain databases

InterProIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR015800. Cu_amine_oxidase_N2.
IPR015801. Cu_amine_oxidase_N2/3.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
Gene3DG3DSA:3.10.450.40. CuNH_oxidase. 2 hits.
G3DSA:2.70.98.20. Lyase_8_central. 1 hit.
PANTHERPTHR10638. CuNH_oxidase. 1 hit.
PfamPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSPR00766. CUDAOXIDASE.
PROSITEPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAOC3_BOVIN
AccessionPrimary (citable) accession number: Q9TTK6
Secondary accession number(s): A5D7R7, Q58D95
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents