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Protein

Membrane primary amine oxidase

Gene

AOC3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity (By similarity).By similarity

Catalytic activityi

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Cu cationBy similarityNote: Binds 1 copper ion per subunit.By similarity
  • Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity
  • L-topaquinoneBy similarityNote: Contains 1 topaquinone per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei386 – 3861Proton acceptorBy similarity
Active sitei471 – 4711Schiff-base intermediate with substrate; via topaquinoneBy similarity
Metal bindingi520 – 5201Copper; via tele nitrogenBy similarity
Metal bindingi522 – 5221Copper; via tele nitrogenBy similarity
Metal bindingi529 – 5291Calcium 1By similarity
Metal bindingi530 – 5301Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi531 – 5311Calcium 1By similarity
Metal bindingi572 – 5721Calcium 2By similarity
Metal bindingi663 – 6631Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi665 – 6651Calcium 2By similarity
Metal bindingi667 – 6671Calcium 2By similarity
Metal bindingi673 – 6731Calcium 1By similarity
Metal bindingi674 – 6741Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi684 – 6841Copper; via pros nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • amine metabolic process Source: UniProtKB
  • cell adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Copper, Metal-binding

Enzyme and pathway databases

SABIO-RKQ9TTK6.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane primary amine oxidase (EC:1.4.3.21By similarity)
Alternative name(s):
Copper amine oxidase
Semicarbazide-sensitive amine oxidase
Short name:
SSAO
Vascular adhesion protein 1
Short name:
VAP-1
Gene namesi
Name:AOC3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 65CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 763736ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3265.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 763762Membrane primary amine oxidasePRO_0000064101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi137 – 1371N-linked (GlcNAc...)By similarity
Disulfide bondi198 ↔ 199By similarity
Glycosylationi212 – 2121O-linked (GalNAc...)By similarity
Glycosylationi232 – 2321N-linked (GlcNAc...)By similarity
Glycosylationi294 – 2941N-linked (GlcNAc...)By similarity
Disulfide bondi404 ↔ 430By similarity
Modified residuei471 – 47112',4',5'-topaquinoneBy similarity
Glycosylationi618 – 6181N-linked (GlcNAc...)By similarity
Glycosylationi666 – 6661N-linked (GlcNAc...)By similarity
Disulfide bondi734 ↔ 741By similarity
Disulfide bondi748 – 748InterchainBy similarity

Post-translational modificationi

N- and O-glycosylated.By similarity
Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, TPQ

Proteomic databases

PaxDbiQ9TTK6.
PeptideAtlasiQ9TTK6.
PRIDEiQ9TTK6.

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Forms a heterodimer with AOC2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000047520.

Structurei

3D structure databases

ProteinModelPortaliQ9TTK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 39411Substrate bindingBy similarityAdd
BLAST
Regioni468 – 4736Substrate bindingBy similarity
Regioni578 – 5858Heparin-bindingBy similarity

Sequence similaritiesi

Belongs to the copper/topaquinone oxidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1186. Eukaryota.
COG3733. LUCA.
HOGENOMiHOG000233919.
HOVERGENiHBG004164.
InParanoidiQ9TTK6.
KOiK00276.

Family and domain databases

Gene3Di2.70.98.20. 1 hit.
InterProiIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
PANTHERiPTHR10638. PTHR10638. 1 hit.
PfamiPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSiPR00766. CUDAOXIDASE.
SUPFAMiSSF49998. SSF49998. 1 hit.
SSF54416. SSF54416. 2 hits.
PROSITEiPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9TTK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNQKTTLVLL ALAVITIFAL VCVLIAGRGG DGGEASQPHY CPSGTPSVQP
60 70 80 90 100
WTHPGQNQLF ADLSREELTA VMSFLTQKLG PDLVDAAQAR PSDNCIFSVE
110 120 130 140 150
LQLPPKAAAL AHLDRRSPPP AREALAIVFF GGQPQPNVTE LVVGPLPQPS
160 170 180 190 200
YMRDVTVERH GGPLPYYRRP VLLREYLDID QMIFNRELPQ AAGVLHHCCS
210 220 230 240 250
YKQGGGNLVT MTTAPRGLQS GDRATWFGLY YNISGAGYYL HPVGLELLVD
260 270 280 290 300
HKALDPAQWT IQKVFFQGRY YESLAQLEEQ FEAGRVNVVV IPNNGTGGSW
310 320 330 340 350
SLKSQVPPGP TPPLQFHPQG TRFSVQGSRV TSSLWTFSFG LGAFSGPRIF
360 370 380 390 400
DIRFQGERLA YEISLQEAVA IYGGNTPAAM LTRYMDGCFG MGKFATPLTR
410 420 430 440 450
GVDCPYLATY VDWHFLLESQ APRTLHDAFC VFEQNKGLPL RRHHSDFISQ
460 470 480 490 500
YFGGVVETVL VFRSVSTLLN YDYVWDMVFH PNGAIEVKFH ATGYISSAFF
510 520 530 540 550
FGTAQKYGNQ VRENTLGTVH THSAHYKVDL DVGGLENWVW AEDMAFVPTT
560 570 580 590 600
VPWSPEHQIQ RLQVTRKQLE TEEQAAFPLG GASPRYLYLA SKQSNKWGHP
610 620 630 640 650
RGYRIQTVSF AGRPLPQNSS TERAISWGRY QLAVTQRKET EPSSSSVFNQ
660 670 680 690 700
NDPWTPTVDF ADFINNETIA GKDLVAWVTA GFLHIPHAED IPNTVTVGNG
710 720 730 740 750
VGFFLRPYNF FDEDPSINSA DSIYFQKHQD AGSCEVNSLA CLPKDPACAP
760
DLPAFSHGGF FTN
Length:763
Mass (Da):84,500
Last modified:January 23, 2007 - v3
Checksum:iDB61ED9A89E71E90
GO
Isoform 2 (identifier: Q9TTK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     734-763: CEVNSLACLPKDPACAPDLPAFSHGGFFTN → SMLPESGASQ...TPRCIFRRHG

Note: No experimental confirmation available.
Show »
Length:783
Mass (Da):86,905
Checksum:i5E213F41CD04EA7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781D → H in AAX46549 (PubMed:16305752).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei734 – 76330CEVNS…GFFTN → SMLPESGASQLCAPQLAGFL SQRRGTVSQEAGVMVTRFYK TPRCIFRRHG in isoform 2. 1 PublicationVSP_016603Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019242 mRNA. Translation: BAA88896.1.
BT021702 mRNA. Translation: AAX46549.1.
BC140657 mRNA. Translation: AAI40658.1.
RefSeqiNP_851345.1. NM_181002.3. [Q9TTK6-1]
UniGeneiBt.105258.
Bt.105260.

Genome annotation databases

GeneIDi281002.
KEGGibta:281002.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019242 mRNA. Translation: BAA88896.1.
BT021702 mRNA. Translation: AAX46549.1.
BC140657 mRNA. Translation: AAI40658.1.
RefSeqiNP_851345.1. NM_181002.3. [Q9TTK6-1]
UniGeneiBt.105258.
Bt.105260.

3D structure databases

ProteinModelPortaliQ9TTK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000047520.

Chemistry

ChEMBLiCHEMBL3265.

Proteomic databases

PaxDbiQ9TTK6.
PeptideAtlasiQ9TTK6.
PRIDEiQ9TTK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281002.
KEGGibta:281002.

Organism-specific databases

CTDi8639.

Phylogenomic databases

eggNOGiKOG1186. Eukaryota.
COG3733. LUCA.
HOGENOMiHOG000233919.
HOVERGENiHBG004164.
InParanoidiQ9TTK6.
KOiK00276.

Enzyme and pathway databases

SABIO-RKQ9TTK6.

Family and domain databases

Gene3Di2.70.98.20. 1 hit.
InterProiIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
PANTHERiPTHR10638. PTHR10638. 1 hit.
PfamiPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSiPR00766. CUDAOXIDASE.
SUPFAMiSSF49998. SSF49998. 1 hit.
SSF54416. SSF54416. 2 hits.
PROSITEiPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of semicarbazide-sensitive amine oxidase gene from Bovine aorta."
    Iwabuki H., Matsumura K., Mure M., Kuroda S., Tanizawa K.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiAOC3_BOVIN
AccessioniPrimary (citable) accession number: Q9TTK6
Secondary accession number(s): A5D7R7, Q58D95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.