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Protein

Lysosomal-trafficking regulator

Gene

LYST

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be required for sorting endosomal resident proteins into late multivesicular endosomes by a mechanism involving microtubules.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal-trafficking regulator
Gene namesi
Name:LYST
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Defects in LYST are the cause of Chediak-Higashi syndrome of cattle (CHS) CHS is an autosomal recessive bleeding disorder with light coat color which has been reported in a population of Japanese black cattle.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 37963796Lysosomal-trafficking regulatorPRO_0000244622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641PhosphoserineBy similarity
Modified residuei165 – 1651PhosphothreonineBy similarity
Modified residuei166 – 1661PhosphoserineBy similarity
Modified residuei1504 – 15041PhosphoserineBy similarity
Modified residuei1505 – 15051PhosphoserineBy similarity
Modified residuei2100 – 21001PhosphoserineBy similarity
Modified residuei2119 – 21191PhosphoserineBy similarity
Modified residuei2208 – 22081PhosphoserineBy similarity
Modified residuei2212 – 22121PhosphoserineBy similarity
Modified residuei2259 – 22591PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9TTK4.
PRIDEiQ9TTK4.

Interactioni

Subunit structurei

Interacts with CENPJ, LIP8 and ZNF521.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022355.

Structurei

3D structure databases

ProteinModelPortaliQ9TTK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati662 – 70039WD 1Add
BLAST
Repeati1577 – 162145WD 2Add
BLAST
Domaini3004 – 3110107BEACH-type PHPROSITE-ProRule annotationAdd
BLAST
Domaini3115 – 3417303BEACHPROSITE-ProRule annotationAdd
BLAST
Repeati3558 – 359740WD 3Add
BLAST
Repeati3609 – 364840WD 4Add
BLAST
Repeati3651 – 369444WD 5Add
BLAST
Repeati3695 – 373945WD 6Add
BLAST
Repeati3744 – 378340WD 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 366Poly-Glu
Compositional biasi2443 – 24475Poly-Leu

Sequence similaritiesi

Contains 1 BEACH domain.PROSITE-ProRule annotation
Contains 1 BEACH-type PH domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
HOGENOMiHOG000113425.
HOVERGENiHBG006300.
InParanoidiQ9TTK4.

Family and domain databases

Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
InterProiIPR000409. BEACH_dom.
IPR030464. LYST.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13743:SF86. PTHR13743:SF86. 2 hits.
PfamiPF02138. Beach. 1 hit.
PF14844. PH_BEACH. 1 hit.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TTK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDSNSLAR EFLTDVNRLC NAVVQRVEAR EEEEEETHMA TLGQYLVHGR
60 70 80 90 100
GFLLLTKLNS IIDQALTCRE ELLTLLLSLL PLVWKIPVQE EKATDFNLPF
110 120 130 140 150
SAGIILTKEK NSSSQRSTQE KLYLEGSAPA GQVSAKVNVF RKSRRQRKTT
160 170 180 190 200
HRYSVRDARK TQLSTSDSEV NSDDKSIVMT KYRRSHVLQH FVKQCPKEDH
210 220 230 240 250
LTAKLNHLST KEQTPPDTMA LENSREIIPR EESNTDILSE PAALSTISHM
260 270 280 290 300
NNSPFDLCHV LLSLLEKVCK FDITLNHNSA LAASVVPTLT EFLAGFGDCC
310 320 330 340 350
NLSDNLEGQM VSAGWTEEPV ALIQRMLFRT VLHLMSVDIS MAEVMPENLR
360 370 380 390 400
KNLTELLRAA LKIRTFLEKQ PDPFAPRQKK TLQEVQDDFV FSKYCHRVLL
410 420 430 440 450
LPELLEGVLQ ILICCLQSAA SNPFFFSQAM DLVQEFIQHH GFHLFETAVL
460 470 480 490 500
QMEWLVVRDG VPPEASGHLK ALINNVMKIM STVKKVKSEQ LHHSMCTRKR
510 520 530 540 550
HRRCEYSHFM HHHRDLSGLL VSAFKNQVSK NPFEETADGD VYYPERCCCI
560 570 580 590 600
AVCAHQCLRL LQQASLSSTC VQILSGVQNI GICCCMDPKS VIVPLLHAFK
610 620 630 640 650
LPALKSCQQH ILNILNKLIL DQLGGAEIPQ KTKKAACNIC TVDSDQLAKL
660 670 680 690 700
EETLQGSSYN AEPSSGLSSP SYRFQGILPS SGSEDLLWKW DALEAYQNFV
710 720 730 740 750
FEEDRLQSVQ IANHICSLIQ KGNVVVQWKL YNCIFNPVLQ RGVELAHHCQ
760 770 780 790 800
QLSISSAQTH VCSHHNQCLP QEVLQIYLKT LPTLLKSRVI RDLFLSCNGV
810 820 830 840 850
NQIIELNYLD GIRNHSLKAF ETLIISLGEQ QKDSSIPGID GIDLEQKELS
860 870 880 890 900
SLNVGTLYNQ QAYSDSQSLS KFYARLKDAY PKKRKSVVQD IHISTINLFL
910 920 930 940 950
CVAFLCVSKE AESDRESAND SEDTSGYDST ASEPLQHMLP CFSLESLVLP
960 970 980 990 1000
SPRHMHQAAD VWSMCRWIYM LSPIFRKQFY RLGGFQVCHK LIFMVIQDLF
1010 1020 1030 1040 1050
RNPEEEQGRK EGDRTMNENQ DLNRISQPEI TVKEDLLSLT VKIDPTPTEL
1060 1070 1080 1090 1100
SSLKKSADSL GRLESEHLSS INVEQIPAVE AVPEETKVFM SRESETLLQG
1110 1120 1130 1140 1150
IRLLEALLAI CLHGTRASQQ KLELELPNQN LSVETILLEM RDHLSKSKVT
1160 1170 1180 1190 1200
ETELAKPLFD ALLRVALGNH SADFEHDDAM TEKSHQSEEE LSSQPGDFSE
1210 1220 1230 1240 1250
EAEDSQCCSF KLLVEEEGYE ADSESNPEDS ETWDDGVDLK PEAESFIASS
1260 1270 1280 1290 1300
SPNDLLENLS QGEIIYPEIC TLELNLLSTG KAKLDVLAHV FESFLKIIRQ
1310 1320 1330 1340 1350
KEKNIFLLMQ QGTVKNLLGG FLSILTQTDS DFQACQRVLV DLLVSLMSSR
1360 1370 1380 1390 1400
TCSEELTLLL RIFLEKSPCT EILLLGILKI VESDITMSPS QYLTFPLLHT
1410 1420 1430 1440 1450
PNLSNGVSSQ KCPGILNSKA MGLLRRARVS QSKKEGDSES FPQQLLSSWH
1460 1470 1480 1490 1500
IAPVHLPLLG QPHLSEGFSI SLWFNVECIH EPESTTEKGK KTRKRNKSLV
1510 1520 1530 1540 1550
LLDSSFDGTE NNRLEGAAYV NPGERLIEEG CVHMISLGSK ALIIQVWADP
1560 1570 1580 1590 1600
HTGTFIFRVC MDSNDDTKVV LLAQVESQEN IFLPSKWQHL VLTYLQQPQG
1610 1620 1630 1640 1650
KKNIHGKISI WISGQRKPDV TLDFMLPRKT SLSSDSNKTF CMIGHCLSSQ
1660 1670 1680 1690 1700
EESLQLAGKW DLGNLLLFNG AKIGSQEAFY LYACGPNHTS IMPCKYGKPV
1710 1720 1730 1740 1750
NDYSKYINTE ILQCEQIREL FMTSKDVDIG LLIESLSVVY TTYCPAQYTI
1760 1770 1780 1790 1800
YEPVIRLKGQ MKTQLSQRPF SSKEVHNILL EPHHLKNLQP TECKTLQGIL
1810 1820 1830 1840 1850
HEIGGTGIFV FLFARVVELS SCEETQALAL RVILSLIKYN QQRIHELENC
1860 1870 1880 1890 1900
NGLSMIHQVL IKQKCIVGFH ILKTLLEGCC GEDIIHINEN GELKLDVESN
1910 1920 1930 1940 1950
AIIQDVKLLE ELLLDWKIWN KAEHGVWETL LAALEVLIRA DHQQQMFNIK
1960 1970 1980 1990 2000
QLLKARVVHH FLLTCQVLQE HKEGQLISMP QEVCRSFVKI TAEVLGSPPD
2010 2020 2030 2040 2050
LELLTIIFNF LLAVHPPTNT YVCHNPTNFY FSLHIDGKIF QEKVQSIMYL
2060 2070 2080 2090 2100
RHSSSGGKSV TSPGFMIISP SDFTASPPEG NNSSSAVPKQ VTASMLRSRS
2110 2120 2130 2140 2150
LPAFPTASPH IKPRKLTGSL GCSIDKLQSF VDDGVASQPE KWSPLRSSET
2160 2170 2180 2190 2200
LEKSKQDAFI SSCESAKMVC ETEPALLAQA SVSDIPKGAL ELPAVNIDHK
2210 2220 2230 2240 2250
DFGAEPRSDD DSPGDESCPR RPLYLKGLAS FQRSHSTIAS LGLAFPSQNG
2260 2270 2280 2290 2300
SAAVGRWPSL VDRNADDWEN FALSLGYEPH HSRTAGAHSV TEDCLVPICC
2310 2320 2330 2340 2350
GLYELLSGVL LILPDVMLED VMDRLIQADT LLVLVNHPSP AVQQEVIKLL
2360 2370 2380 2390 2400
DAYFNRASKE QKDKFLKNRG FSLLANQLYL HRGTQELLEC FIEMFFGRRI
2410 2420 2430 2440 2450
GLDEEFDLED VKNMGLFQKW SVIPILGLIE TSLYDNVLLH NALLLLLQIL
2460 2470 2480 2490 2500
NSCSKVADML LDNGLLYVLC NTVATLNGLE KIIPLNEYRL LACDIQQLFI
2510 2520 2530 2540 2550
AVTIHACSSS GSQYFRVIED LIVLLGYLQN SKNKRTQNMA VALQFRVLQA
2560 2570 2580 2590 2600
AMEFIRTTAN HDSENLTDSL QSPSVPHHTM FQKRKSIAGP RKFLLAQTDS
2610 2620 2630 2640 2650
LLMKMRSVAS DELHVMMQRR MSQENPVQAT ETELAQRLQR LTVFAVNRIV
2660 2670 2680 2690 2700
YQEFNPDIID ILSTPESTTQ SRTSASQTEI SEENIHHEQP SVFNPFQKEM
2710 2720 2730 2740 2750
FMYLVEGFKV SSASGKTSSS KQQWAKILWS CKETFRMQLG RLLVHMLSPA
2760 2770 2780 2790 2800
HPSQERKQIF EIVREPNHQE ILRECLSPSL QHGAKLVLYL SELIHNHKDE
2810 2820 2830 2840 2850
LTEEELDTAE LLMNALKLCG HKCIPPSAST KSDLIRVIKE EQRKYETEEG
2860 2870 2880 2890 2900
VNKANWQKTV NNNQQSLFQR LDSKSKDISK IAADITQAVS LSQGIERKKV
2910 2920 2930 2940 2950
IQHIRGMYKV DLSASRHWQE LIQQLTHDRA VWYDPIYYPT SWQLDPTEGP
2960 2970 2980 2990 3000
NRERRRLQRC YLTIPNKYLL RDRQKSEDVV KPPLSYLFED KTHSSFSSTV
3010 3020 3030 3040 3050
KDKAASESIR VNRRCISVAP SRETAGELLL GKCGMYFVED NASDTVECSN
3060 3070 3080 3090 3100
LQGELEPASF SWTYEEIKEV HKRWWQLRDN AVEIFLTNGR TLLLAFDNTK
3110 3120 3130 3140 3150
VRNDVYHSIL TNNLPNLLEY GNITALTHLW YTGQITNFEY LTHLNKHAGR
3160 3170 3180 3190 3200
SFNDLMQYPV FPFILADYVS ETLDLSDPSV YRNLSKPIAV QYKEKEDRYV
3210 3220 3230 3240 3250
DTYKYLEEEY RKGAREDDPM PPVQPYHYGS HYSNSGTVLH FLVRMPPFTK
3260 3270 3280 3290 3300
MFLAYQDQSF DIPDRTFHST NTTWRLSSFE SMTDVKELIP EFFYLPEFLV
3310 3320 3330 3340 3350
NREGFDFGVR QNGERVNHVN LPPWARNDPR LFILIHRQAL ESDYVSQNIC
3360 3370 3380 3390 3400
QWIDLVFGYK QKGKASVQAI NVFHPATYFG MDVSAVEDPV QRRALETMIK
3410 3420 3430 3440 3450
TYGQTPRQLF QSAHASRPGS KLNIEGELPA AVGLLVQFAF RETREQVKEI
3460 3470 3480 3490 3500
TYPSPLSWIK GLKWGEYVGS PSAPVPVVCF SQPHGERFGS LQALPTRAIC
3510 3520 3530 3540 3550
GLSRNFCLLM TYSKEQGVRS MNSTDIQWSA ILSWGYADNI LRLKSKQSEP
3560 3570 3580 3590 3600
PINFIQSSQQ YQVTSCAWVP DSCQLFTGSK CGVITAYSNR FTSGTPSEIE
3610 3620 3630 3640 3650
MESQIHLYGH TEEISSLFVC KPYSIMISVS RDGTCIIWDL NRLCYVQSLA
3660 3670 3680 3690 3700
GHKSPVTAVS ASETTGDIAT VCDSAGGGSD LRLWTVNGDL VGHVHCREII
3710 3720 3730 3740 3750
CSVAFSNQPE GISINVIAGG LENGIVRLWS TWDLKPVREI TFPKSNKPIV
3760 3770 3780 3790
SLTFSCDGHH LYTANSDGTV IAWCRKDQQR LKHPMFYSFL SSYAAG
Length:3,796
Mass (Da):428,601
Last modified:May 1, 2000 - v1
Checksum:i4AC00E9928E882B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1062 – 10621R → K in AAD13618 (PubMed:10690356).Curated
Sequence conflicti1434 – 14341K → I in AAD13618 (PubMed:10690356).Curated
Sequence conflicti2060 – 20601V → G in AAD13618 (PubMed:10690356).Curated
Sequence conflicti3396 – 33961E → R in AAD13618 (PubMed:10690356).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2015 – 20151H → R in CHS. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026620 mRNA. Translation: BAA77267.1.
AF114785 mRNA. Translation: AAD13618.1.
PIRiT18514.
RefSeqiNP_776445.1. NM_174020.2.
UniGeneiBt.4767.

Genome annotation databases

GeneIDi281072.
KEGGibta:281072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026620 mRNA. Translation: BAA77267.1.
AF114785 mRNA. Translation: AAD13618.1.
PIRiT18514.
RefSeqiNP_776445.1. NM_174020.2.
UniGeneiBt.4767.

3D structure databases

ProteinModelPortaliQ9TTK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022355.

Proteomic databases

PaxDbiQ9TTK4.
PRIDEiQ9TTK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281072.
KEGGibta:281072.

Organism-specific databases

CTDi1130.

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
HOGENOMiHOG000113425.
HOVERGENiHBG006300.
InParanoidiQ9TTK4.

Family and domain databases

Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
InterProiIPR000409. BEACH_dom.
IPR030464. LYST.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13743:SF86. PTHR13743:SF86. 2 hits.
PfamiPF02138. Beach. 1 hit.
PF14844. PH_BEACH. 1 hit.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of bovine LYST gene and identification of a missense mutation associated with Chediak-Higashi syndrome of cattle."
    Kunieda T., Nakagiri M., Takami M., Ide H., Ogawa H.
    Mamm. Genome 10:1146-1149(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CHS ARG-2015.
    Tissue: Brain.
  2. "Chediak-Higashi syndrome mutation and genetic testing in Japanese black cattle (Wagyu)."
    Yamakuchi H., Agaba M., Hirano T., Hara K., Todoroki J., Mizoshita K., Kubota C., Tabara N., Sugimoto Y.
    Anim. Genet. 31:13-19(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiLYST_BOVIN
AccessioniPrimary (citable) accession number: Q9TTK4
Secondary accession number(s): O97699
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.