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Protein

Regucalcin

Gene

RGN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).By similarity

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.

Cofactori

Zn2+By similarity, Mn2+By similarity, Ca2+By similarity, Mg2+By similarityNote: Binds 1 divalent metal cation per subunit. Most active with Zn2+ and Mn2+ ions. The physiological cofactor is most likely Ca2+ or Mg2+.By similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 3 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (RGN)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Divalent metal cationBy similarity
Binding sitei101 – 1011SubstrateBy similarity
Binding sitei103 – 1031SubstrateBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Metal bindingi154 – 1541Divalent metal cationBy similarity
Active sitei204 – 2041Proton donor/acceptorBy similarity
Metal bindingi204 – 2041Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00991; UER00938.

Names & Taxonomyi

Protein namesi
Recommended name:
Regucalcin
Short name:
RC
Alternative name(s):
Gluconolactonase (EC:3.1.1.17)
Short name:
GNL
Senescence marker protein 30
Short name:
SMP-30
Gene namesi
Name:RGN
Synonyms:SMP30
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299RegucalcinPRO_0000173048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei144 – 1441N6-succinyllysineBy similarity
Modified residuei244 – 2441N6-succinyllysineBy similarity
Modified residuei253 – 2531N6-succinyllysineBy similarity

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022189.

Structurei

3D structure databases

ProteinModelPortaliQ9TTJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SMP-30/CGR1 family.Curated

Phylogenomic databases

eggNOGiKOG4499. Eukaryota.
COG3386. LUCA.
GeneTreeiENSGT00390000014995.
HOGENOMiHOG000220627.
HOVERGENiHBG004347.
InParanoidiQ9TTJ6.
KOiK01053.
OMAiCARDGMD.
OrthoDBiEOG77127C.
TreeFamiTF323663.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR008367. Regucalcin.
IPR013658. SGL.
IPR005511. SMP-30.
[Graphical view]
PfamiPF08450. SGL. 1 hit.
[Graphical view]
PRINTSiPR01791. REGUCALCIN.
PR01790. SMP30FAMILY.

Sequencei

Sequence statusi: Complete.

Q9TTJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIKIECVL PENCHCGESP VWEEASGSLL FVDIPGKKFC RWNPLTKAVQ
60 70 80 90 100
RMTMDAPVTS VALRKSGGYV ATVGTKFCAL NLEDQSVVAL ATVDKDKKNN
110 120 130 140 150
RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGSLYALFPD HQVKKYFDQV
160 170 180 190 200
DISNGLDWSL DHKIFYYIDS LAYSVDAFDY DLQTGQISNR RSIYKLEKEE
210 220 230 240 250
QIPDGMCIDT EGKLWVACYN GGRVIRLDPE TGKRLQTVKL PVDKTTSCCF
260 270 280 290
GGKDYSEMYV TCARDGLDPD SLSRQPEAGG IFKITGLGVK GIPPYSYAG
Length:299
Mass (Da):33,109
Last modified:May 1, 2000 - v1
Checksum:iA8F94AD6596A0C68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035445 mRNA. Translation: BAA88079.1.
RefSeqiNP_001075472.1. NM_001082003.1.
XP_008270558.1. XM_008272336.1.
UniGeneiOcu.2460.

Genome annotation databases

EnsembliENSOCUT00000028528; ENSOCUP00000022189; ENSOCUG00000023725.
GeneIDi100008619.
KEGGiocu:100008619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035445 mRNA. Translation: BAA88079.1.
RefSeqiNP_001075472.1. NM_001082003.1.
XP_008270558.1. XM_008272336.1.
UniGeneiOcu.2460.

3D structure databases

ProteinModelPortaliQ9TTJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000028528; ENSOCUP00000022189; ENSOCUG00000023725.
GeneIDi100008619.
KEGGiocu:100008619.

Organism-specific databases

CTDi9104.

Phylogenomic databases

eggNOGiKOG4499. Eukaryota.
COG3386. LUCA.
GeneTreeiENSGT00390000014995.
HOGENOMiHOG000220627.
HOVERGENiHBG004347.
InParanoidiQ9TTJ6.
KOiK01053.
OMAiCARDGMD.
OrthoDBiEOG77127C.
TreeFamiTF323663.

Enzyme and pathway databases

UniPathwayiUPA00991; UER00938.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR008367. Regucalcin.
IPR013658. SGL.
IPR005511. SMP-30.
[Graphical view]
PfamiPF08450. SGL. 1 hit.
[Graphical view]
PRINTSiPR01791. REGUCALCIN.
PR01790. SMP30FAMILY.
ProtoNetiSearch...

Publicationsi

  1. "The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species."
    Misawa H., Yamaguchi M.
    Int. J. Mol. Med. 6:191-196(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiRGN_RABIT
AccessioniPrimary (citable) accession number: Q9TTJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.