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Protein

Calpain-3

Gene

CAPN3

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291By similarity
Active sitei335 – 3351By similarity
Active sitei359 – 3591By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi706 – 717121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi736 – 747122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54. 2668.

Protein family/group databases

MEROPSiC02.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Muscle-specific calcium-activated neutral protease 3
New calpain 1
Short name:
nCL-1
Gene namesi
Name:CAPN3
Synonyms:NCL1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 822822Calpain-3PRO_0000207711Add
BLAST

Expressioni

Tissue specificityi

Skeletal muscle.

Interactioni

Subunit structurei

Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9TTH8.
SMRiQ9TTH8. Positions 60-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 418345Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini650 – 68435EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini693 – 72634EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini723 – 75836EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini788 – 82235EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni419 – 587169Domain IIIAdd
BLAST
Regioni588 – 64962LinkerAdd
BLAST
Regioni650 – 822173Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
KOiK08573.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TTH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVISASVA PRTGAEPRSP GPIAQAAQGK GTEAGGGNPS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHRKCLE KKVLFVDPEF PPDETSLFYS QKFPIQFIWK
110 120 130 140 150
RPPEICENPR FIIGGANRTD ICQGDLGDCW FLAAIACLTL NERLLFRVIP
160 170 180 190 200
HDQSFTENYA GIFHFQFWRY GDWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WSALLEKAYA KLHGSYEALK AGNTTEGMED FTGELTEFFE IKDAPRDMYK
260 270 280 290 300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LKMGELIERM VRNMDNSRLR
310 320 330 340 350
DSDLIPEGCS DDRPTRTIVP VQYETRMACG LVKGHAYSVT GLEEALYKGE
360 370 380 390 400
KVKLVRLRNP WGQVEWNGSW SDSWKDWSYV DKDEKARLQH QVTEDGEFWM
410 420 430 440 450
SYDDFIYHFT KLEICNLTAD ALESDKLQTW TVSVNEGRWV RGCSAGGCRN
460 470 480 490 500
FPDTFWTNPQ YRLKLLEEDD DPDDSEVICS FLVALMQKNR RKDRKLGANL
510 520 530 540 550
FTIGFAIYEV PKEMHGNKQH LQKDFFLYNA SKARSRTYIN MREVSERFRL
560 570 580 590 600
PPSEYVIVPS TYEPHQEGEF ILRVFSEKRN LSEEVENTIS VDRPVKKKKP
610 620 630 640 650
KPIIFGSDRA NSNKELGVDQ ESEEGKDNTS PDKQAKSPQL KPGNIDQESK
660 670 680 690 700
EQRQFRNIFR QIAGDDMEIC ADELKNVLNR VVNKHKDLKT QGFTLESCRS
710 720 730 740 750
MIALMDTDGS GRLNLQEFHH LWKKIKTWQK IFKHYDTDQS GTINSYEMRN
760 770 780 790 800
AVNDAGFHLN NQLYDIITMR YADKYMNIDF DSFICCFVRL EGMFRAFNAF
810 820
DKDGDGIIKL NVLEWLQLTM YA
Length:822
Mass (Da):94,595
Last modified:May 1, 2000 - v1
Checksum:iE78F63BCEBC68D7E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371G → A in AAD05334 (PubMed:11204692).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087570 mRNA. Translation: AAF23262.1.
AF115745 mRNA. Translation: AAD05334.1.
RefSeqiNP_001009212.1. NM_001009212.1.
UniGeneiOar.716.

Genome annotation databases

GeneIDi443038.
KEGGioas:443038.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087570 mRNA. Translation: AAF23262.1.
AF115745 mRNA. Translation: AAD05334.1.
RefSeqiNP_001009212.1. NM_001009212.1.
UniGeneiOar.716.

3D structure databases

ProteinModelPortaliQ9TTH8.
SMRiQ9TTH8. Positions 60-418.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC02.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443038.
KEGGioas:443038.

Organism-specific databases

CTDi825.

Phylogenomic databases

HOVERGENiHBG012645.
KOiK08573.

Enzyme and pathway databases

BRENDAi3.4.22.54. 2668.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and mapping of the bovine and ovine skeletal muscle-specific calpains."
    Nonneman D., Koohmaraie M.
    Anim. Genet. 30:456-458(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "Intermuscular variation in tenderness: association with the ubiquitous and muscle-specific calpains."
    Ilian M.A., Morton J.D., Kent M.P., Le Couteur C.E., Hickford J., Cowley R., Bickerstaffe R.
    J. Anim. Sci. 79:122-132(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 288-605.

Entry informationi

Entry nameiCAN3_SHEEP
AccessioniPrimary (citable) accession number: Q9TTH8
Secondary accession number(s): O97701
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 1, 2000
Last modified: January 20, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.