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Q9TTC0

- ENV_KORV

UniProt

Q9TTC0 - ENV_KORV

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Protein

Envelope glycoprotein

Gene
env
Organism
Koala retrovirus (KoRV)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane By similarity.
The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei463 – 4642Cleavage; by host By similarity
Sitei643 – 6442Cleavage; by viral protease By similarity

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Alternative name(s):
Env polyprotein
Cleaved into the following 3 chains:
Surface protein
Short name:
SU
Alternative name(s):
Glycoprotein 70
Short name:
gp70
Transmembrane protein
Short name:
TM
Alternative name(s):
Envelope protein p15E
Alternative name(s):
p2E
Gene namesi
Name:env
OrganismiKoala retrovirus (KoRV)
Taxonomic identifieri394239 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiPhascolarctos cinereus (Koala) [TaxID: 38626]
ProteomesiUP000007765: Genome

Subcellular locationi

Chain Surface protein : Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein By similarity
Note: The surface protein is not anchored to the viral envelope, but associates with the extravirion surface through its binding to TM. Both proteins are thought to be concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag By similarity.
R-peptide : Host cell membrane; Peripheral membrane protein By similarity
Note: The R-peptide is membrane-associated through its palmitate By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 606571Extracellular Reviewed predictionAdd
BLAST
Transmembranei607 – 62721Helical; Reviewed predictionAdd
BLAST
Topological domaini628 – 65932Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral capsid Source: InterPro
  4. viral envelope Source: UniProtKB-KW
  5. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535 Reviewed predictionAdd
BLAST
Chaini36 – 659624Envelope glycoproteinPRO_0000249423Add
BLAST
Chaini36 – 463428Surface protein By similarityPRO_0000249424Add
BLAST
Chaini464 – 659196Transmembrane protein By similarityPRO_0000249425Add
BLAST
Peptidei645 – 65915R-peptide By similarityPRO_0000249426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi141 ↔ 162 By similarity
Disulfide bondi154 ↔ 167 By similarity
Glycosylationi318 – 3181N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi328 ↔ 558Interchain (between SU and TM chains, or C-357 with C-584); in linked form By similarity
Disulfide bondi328 ↔ 331 By similarity
Glycosylationi389 – 3891N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi395 – 3951N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi407 – 4071N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi427 – 4271N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi550 ↔ 557 By similarity
Lipidationi625 – 6251S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as a inactive precursor that is N-glycosylated and processed likely by host cell furin or by a furin-like protease in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R. The R-peptide is released from the C-terminus of the cytoplasmic tail of the TM protein upon particle formation as a result of proteolytic cleavage by the viral protease. Cleavage of this peptide is required for TM to become fusogenic By similarity.
The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion By similarity.
The transmembrane protein is palmitoylated By similarity.
The R-peptide is palmitoylated By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached by a labile interchain disulfide bond By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9TTC0.
SMRiQ9TTC0. Positions 510-562.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni466 – 48621Fusion peptide By similarityAdd
BLAST
Regioni533 – 54917Immunosuppression By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili506 – 53227 Reviewed predictionAdd
BLAST
Coiled coili567 – 58721 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi328 – 3314CXXC By similarity
Motifi550 – 5589CX6CC By similarity
Motifi650 – 6534YXXL motif; contains endocytosis signal By similarity

Domaini

The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo By similarity.
The YXXL motif is involved in determining the exact site of viral release at the surface of infected mononuclear cells and promotes endocytosis By similarity.

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.310.10. 1 hit.
InterProiIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERiPTHR10424. PTHR10424. 1 hit.
PfamiPF00429. TLV_coat. 1 hit.
[Graphical view]
SUPFAMiSSF49830. SSF49830. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9TTC0-1 [UniParc]FASTAAdd to Basket

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MLLISNPRHL GHPMSPGNWK RLIILLSCVF GGAEMNQQHN NPHQPMTLTW    50
QVLSQTGSVV WEKKAVEPPW TWWPSLEPDV CALVAGLESW DIPELTASAS 100
QQARPPDSNY EHAYNQITWG TLGCSYPRAR TRIARSQFYV CPRDGRSLSE 150
ARRCGGLESL YCKEWGCETA GTAYWQPRSS WDLITVGQGH PTGTCERTGW 200
CNPLKIEFTE PGKRFRNWLQ GRTWGLRFYV TGHPGVQLTI RLVITSPPPV 250
VVGPDPVLAE QGPPRKIPFL PRVPVPTLSP PASPIPTVQA SPPAPSTPSP 300
TTGDRLFGLV QGAFLALNAT NPEATESCWL CLALGPPYYE GIATPGQVTY 350
ASTDSQCRWG GKGKLTLTEV SGLGLCIGKV PPTHQHLCNL TIPLNASHTH 400
KYLLPSNRSW WACNSGLTPC LSTSVFNQSN DFCIQIQLVP RIYYHPDGTL 450
LQAYESPHSR NKREPVSLTL AVLLGLGVAA GIGTGSTALI KGPIDLQQGL 500
TSLQIAMDTD LRALQDSISK LEDSLTSLSE VVLQNRRGLD LLFLKEGGLC 550
AALKEECCFY VDHSGAVRDS MRRLKERLDK RQLEHQKNLS WYEGWFNRSP 600
WLTTLLSALA GPLLLLLLLL TLGPCVINKL VQFINDRVSA VRILVLRHKY 650
QTLDNEDNL 659
Length:659
Mass (Da):72,860
Last modified:May 1, 2000 - v1
Checksum:i68B2220DAA6A2ABA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF151794 Genomic DNA. Translation: AAF15099.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF151794 Genomic DNA. Translation: AAF15099.1 .

3D structure databases

ProteinModelPortali Q9TTC0.
SMRi Q9TTC0. Positions 510-562.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.310.10. 1 hit.
InterProi IPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view ]
PANTHERi PTHR10424. PTHR10424. 1 hit.
Pfami PF00429. TLV_coat. 1 hit.
[Graphical view ]
SUPFAMi SSF49830. SSF49830. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon ape leukemia virus."
    Hanger J.J., Bromham L.D., McKee J.J., O'Brien T.M., Robinson W.F.
    J. Virol. 74:4264-4272(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiENV_KORV
AccessioniPrimary (citable) accession number: Q9TTC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Koala retrovirus is both a circulating virus and an endogenous retrovirus of koala, except in some isolated populations in south Australia.

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi