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Q9TT93

- ATS4_BOVIN

UniProt

Q9TT93 - ATS4_BOVIN

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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 4

Gene

ADAMTS4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Catalytic activityi

Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu-|-Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941Zinc; in inhibited formBy similarity
Metal bindingi361 – 3611Zinc; catalyticBy similarity
Active sitei362 – 3621PROSITE-ProRule annotation
Metal bindingi365 – 3651Zinc; catalyticBy similarity
Metal bindingi371 – 3711Zinc; catalyticBy similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. metallopeptidase activity Source: AgBase
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.221.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 4 (EC:3.4.24.82)
Short name:
ADAM-TS 4
Short name:
ADAM-TS4
Short name:
ADAMTS-4
Alternative name(s):
ADMP-1
Aggrecanase-1
Gene namesi
Name:ADAMTS4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5151Sequence AnalysisAdd
BLAST
Propeptidei52 – 2121611 PublicationPRO_0000239802Add
BLAST
Chaini213 – 839627A disintegrin and metalloproteinase with thrombospondin motifs 4PRO_0000078209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi293 ↔ 345By similarity
Disulfide bondi322 ↔ 327By similarity
Disulfide bondi339 ↔ 423By similarity
Disulfide bondi377 ↔ 407By similarity
Disulfide bondi449 ↔ 472By similarity
Disulfide bondi460 ↔ 482By similarity
Disulfide bondi467 ↔ 501By similarity
Disulfide bondi495 ↔ 506By similarity
Disulfide bondi532 ↔ 569By similarity
Disulfide bondi536 ↔ 574By similarity
Disulfide bondi547 ↔ 559By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ9TT93.

Expressioni

Inductioni

By interleukin-1.

Interactioni

Subunit structurei

Interacts with SRPX2.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9TT93.
SMRiQ9TT93. Positions 215-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini218 – 428211Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini437 – 51983DisintegrinAdd
BLAST
Domaini520 – 57556TSP type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni686 – 839154SpacerBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi192 – 1998Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi247 – 2526Poly-Ala
Compositional biasi577 – 685109Cys-richAdd
BLAST

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271890.
HOGENOMiHOG000004799.
HOVERGENiHBG004313.
InParanoidiQ9TT93.
KOiK07764.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9TT93-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHMDSHPGR GLADGWLWGI QPRLLLPTVP VSGSRLVWLL LLASLLPSAW
60 70 80 90 100
PASPLPREEE IVFPEKLNGS VLPGLGAPAR LLYRLPAFGE TLLLELEKDP
110 120 130 140 150
GVQVEGLTVQ YLGRAPELLG GAEPGTYLTG TINGDPESVA SLHWDGGALL
160 170 180 190 200
GVLQYRGTEL HIQPLEGGAP NSAGGPGAHI LRRKSPVSGQ GPMCNVKAPP
210 220 230 240 250
GKPSPSPRRA KRFASLSRFV ETLVVADDKM AAFHGAGLKR YLLTVMAAAA
260 270 280 290 300
KAFKHPSIRN PVSLVVTRLV VLGPGEEGPQ VGPSAAQTLR SFCAWQRGLN
310 320 330 340 350
TPDDADPGHF DTAILFTRQD LCGVSTCDTL GMADVGTVCD PARSCAIVED
360 370 380 390 400
DGLQSAFTAA HELGHVFSML HDNSKQCTGL NGPESTSRHV MAPVMAHVDP
410 420 430 440 450
EEPWSPCSAR FITDFLDNGF GHCLLDKPEA PLHLPVTFPG KDYDADRQCQ
460 470 480 490 500
LTFGPDSRHC PQLPPPCAAL WCSGHLNGHA MCQTKHSPWA DGTPCGPAQA
510 520 530 540 550
CMGGRCLHVD QLQAFNVPQA GGWGPWGSWG DCSRSCGGGV QFSSRDCTRP
560 570 580 590 600
VPRNGGKYCE GRRTRFRSCN TQDCPTGSAL TFREEQCAAY NHRTDLFKNF
610 620 630 640 650
PGPMDWVPRY TGVAPRDQCK LTCQTRALGY YYVLDPRVAD GTPCSPDSSS
660 670 680 690 700
VCVQGRCIHA GCDRVIGSKK KFDKCMVCGG DGSSCSKQSG SFKKFRYGYN
710 720 730 740 750
NVVTIPAGAT HILVRQQGSP SVRSLYLALK LPDGSYALNG EYTLIPSPTD
760 770 780 790 800
VVLPGAVSLR YSGATAASET LSGHGPLAEP LTLQVLVAGN PQNARLRYSF
810 820 830
FVPRPRPVPS TPRPTPQDWL RRKSQILEIL RRRSWAGRK
Length:839
Mass (Da):90,280
Last modified:June 13, 2006 - v3
Checksum:i1B488A27DF5B96B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516915 mRNA. Translation: AAP47196.1.
BC148059 mRNA. Translation: AAI48060.1.
AF192770 mRNA. Translation: AAF07176.1.
RefSeqiNP_858053.1. NM_181667.1.
UniGeneiBt.15675.

Genome annotation databases

GeneIDi286806.
KEGGibta:286806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516915 mRNA. Translation: AAP47196.1 .
BC148059 mRNA. Translation: AAI48060.1 .
AF192770 mRNA. Translation: AAF07176.1 .
RefSeqi NP_858053.1. NM_181667.1.
UniGenei Bt.15675.

3D structure databases

ProteinModelPortali Q9TT93.
SMRi Q9TT93. Positions 215-507.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q9TT93.
ChEMBLi CHEMBL3874.

Protein family/group databases

MEROPSi M12.221.

Proteomic databases

PRIDEi Q9TT93.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 286806.
KEGGi bta:286806.

Organism-specific databases

CTDi 9507.

Phylogenomic databases

eggNOGi NOG271890.
HOGENOMi HOG000004799.
HOVERGENi HBG004313.
InParanoidi Q9TT93.
KOi K07764.

Miscellaneous databases

NextBioi 20806460.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view ]
Pfami PF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view ]
PRINTSi PR01857. ADAMTSFAMILY.
SMARTi SM00209. TSP1. 1 hit.
[Graphical view ]
SUPFAMi SSF82895. SSF82895. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of bovine aggrecanase-1."
    Arai M., Anderson D., Annis B., Collins-Racie L., Corcoran C., DiBlasio-Smith E., Morris E., Dorner A., LaVallie E.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 332-531.
    Tissue: Cartilage.
  4. "n-3 fatty acids specifically modulate catabolic factors involved in articular cartilage degradation."
    Curtis C.L., Hughes C.E., Flannery C.R., Little C.B., Harwood J.L., Caterson B.
    J. Biol. Chem. 275:721-724(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 332-531.
    Tissue: Cartilage chondrocyte.
  5. Cited for: PROTEIN SEQUENCE OF 213-239; 610-615 AND 626-637.
    Tissue: Cartilage.

Entry informationi

Entry nameiATS4_BOVIN
AccessioniPrimary (citable) accession number: Q9TT93
Secondary accession number(s): A6QLR5, Q7YS95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Has sometimes been referred to as ADAMTS2.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3