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Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi230 – 28960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  4. transcription corepressor activity Source: Ensembl

GO - Biological processi

  1. ectodermal cell fate commitment Source: Ensembl
  2. endodermal cell fate commitment Source: Ensembl
  3. germ-line stem cell maintenance Source: Ensembl
  4. mesodermal cell fate commitment Source: Ensembl
  5. mRNA transcription from RNA polymerase II promoter Source: Ensembl
  6. negative regulation of calcium ion-dependent exocytosis Source: Ensembl
  7. negative regulation of protein kinase B signaling Source: Ensembl
  8. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  9. positive regulation of protein kinase B signaling Source: Ensembl
  10. regulation of asymmetric cell division Source: Ensembl
  11. response to retinoic acid Source: Ensembl
  12. trophectodermal cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_345943. Transcriptional regulation of pluripotent stem cells.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Chromosome 7

Subcellular locationi

Cytoplasm. Nucleus
Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nuclear transcription factor complex Source: Ensembl
  3. nucleolus Source: Ensembl
  4. nucleoplasm Source: Ensembl
  5. transcriptional repressor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360POU domain, class 5, transcription factor 1PRO_0000100751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity
Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei235 – 2351PhosphothreonineBy similarity
Modified residuei236 – 2361PhosphoserineBy similarity
Modified residuei289 – 2891PhosphoserineBy similarity
Modified residuei290 – 2901PhosphoserineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).By similarity
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9TSV5.
SMRiQ9TSV5. Positions 144-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 21275POU-specificPROSITE-ProRule annotationAdd
BLAST

Domaini

The POU-specific domain mediates interaction with PKM.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiQ9TSV5.
KOiK09367.
OMAiLENMFLQ.
OrthoDBiEOG7DJSMG.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TSV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGSGIGPG
60 70 80 90 100
VGPGAEVWGL PACPPPYDFC GGMAYCAPQV GVGLVPQGGL ETPQPEGEAG
110 120 130 140 150
AGVESNSEGA SPEPCAAPAG AAKLDKEKLE PNPEESQDIK ALQKDLEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA
310 320 330 340 350
AGSPFPGGPV SFPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEAFPSVSV
360
TPLGSPMHSN
Length:360
Mass (Da):38,342
Last modified:May 1, 2000 - v1
Checksum:i9BEC167A66409A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251914 Genomic DNA. Translation: CAB63862.1.
RefSeqiNP_001106531.1. NM_001113060.1.
UniGeneiSsc.29069.

Genome annotation databases

EnsembliENSSSCT00000001516; ENSSSCP00000001474; ENSSSCG00000001393.
GeneIDi100127461.
KEGGissc:100127461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251914 Genomic DNA. Translation: CAB63862.1.
RefSeqiNP_001106531.1. NM_001113060.1.
UniGeneiSsc.29069.

3D structure databases

ProteinModelPortaliQ9TSV5.
SMRiQ9TSV5. Positions 144-289.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000001516; ENSSSCP00000001474; ENSSSCG00000001393.
GeneIDi100127461.
KEGGissc:100127461.

Organism-specific databases

CTDi5460.

Phylogenomic databases

eggNOGiNOG329627.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiQ9TSV5.
KOiK09367.
OMAiLENMFLQ.
OrthoDBiEOG7DJSMG.
TreeFamiTF316413.

Enzyme and pathway databases

ReactomeiREACT_345943. Transcriptional regulation of pluripotent stem cells.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the swine major histocompatibility complex region containing all non-classical class I genes."
    Chardon P., Rogel-Gaillard C., Cattolico L., Duprat S., Vaiman M., Renard C.
    Tissue Antigens 57:55-65(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Large white.
    Tissue: Fibroblast.
  2. "Induced pluripotent stem cells from swine (Sus scrofa): why they may prove to be important."
    Roberts R.M., Telugu B.P., Ezashi T.
    Cell Cycle 8:3078-3081(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY, FUNCTION.

Entry informationi

Entry nameiPO5F1_PIG
AccessioniPrimary (citable) accession number: Q9TSV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.