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Q9TSV5

- PO5F1_PIG

UniProt

Q9TSV5 - PO5F1_PIG

Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi230 – 28960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. sequence-specific DNA binding Source: Ensembl
    3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: Ensembl
    4. transcription corepressor activity Source: Ensembl
    5. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. ectodermal cell fate commitment Source: Ensembl
    2. endodermal cell fate commitment Source: Ensembl
    3. germ-line stem cell maintenance Source: Ensembl
    4. mesodermal cell fate commitment Source: Ensembl
    5. mRNA transcription from RNA polymerase II promoter Source: Ensembl
    6. negative regulation of calcium ion-dependent exocytosis Source: Ensembl
    7. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    8. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    9. regulation of asymmetric cell division Source: Ensembl
    10. response to retinoic acid Source: Ensembl
    11. trophectodermal cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_205627. Transcriptional regulation of pluripotent stem cells.
    REACT_216518. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1
    Alternative name(s):
    Octamer-binding protein 3
    Short name:
    Oct-3
    Octamer-binding protein 4
    Short name:
    Oct-4
    Octamer-binding transcription factor 3
    Short name:
    OTF-3
    Gene namesi
    Name:POU5F1
    Synonyms:OCT3, OCT4
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 7

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleoplasm Source: Ensembl
    3. transcriptional repressor complex Source: Ensembl
    4. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Biotechnological usei

    POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360POU domain, class 5, transcription factor 1PRO_0000100751Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity
    Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei235 – 2351PhosphothreonineBy similarity
    Modified residuei236 – 2361PhosphoserineBy similarity
    Modified residuei289 – 2891PhosphoserineBy similarity
    Modified residuei290 – 2901PhosphoserineBy similarity
    Modified residuei355 – 3551PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.By similarity
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
    ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9TSV5.
    SMRiQ9TSV5. Positions 144-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 21275POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The POU-specific domain mediates interaction with PKM.By similarity

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG329627.
    GeneTreeiENSGT00750000117482.
    HOGENOMiHOG000089941.
    HOVERGENiHBG057998.
    KOiK09367.
    OMAiLENMFLQ.
    OrthoDBiEOG7DJSMG.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9TSV5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGSGIGPG    50
    VGPGAEVWGL PACPPPYDFC GGMAYCAPQV GVGLVPQGGL ETPQPEGEAG 100
    AGVESNSEGA SPEPCAAPAG AAKLDKEKLE PNPEESQDIK ALQKDLEQFA 150
    KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL 200
    RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL 250
    QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA 300
    AGSPFPGGPV SFPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEAFPSVSV 350
    TPLGSPMHSN 360
    Length:360
    Mass (Da):38,342
    Last modified:May 1, 2000 - v1
    Checksum:i9BEC167A66409A9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ251914 Genomic DNA. Translation: CAB63862.1.
    RefSeqiNP_001106531.1. NM_001113060.1.
    UniGeneiSsc.29069.

    Genome annotation databases

    EnsembliENSSSCT00000001516; ENSSSCP00000001474; ENSSSCG00000001393.
    GeneIDi100127461.
    KEGGissc:100127461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ251914 Genomic DNA. Translation: CAB63862.1 .
    RefSeqi NP_001106531.1. NM_001113060.1.
    UniGenei Ssc.29069.

    3D structure databases

    ProteinModelPortali Q9TSV5.
    SMRi Q9TSV5. Positions 144-289.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000001516 ; ENSSSCP00000001474 ; ENSSSCG00000001393 .
    GeneIDi 100127461.
    KEGGi ssc:100127461.

    Organism-specific databases

    CTDi 5460.

    Phylogenomic databases

    eggNOGi NOG329627.
    GeneTreei ENSGT00750000117482.
    HOGENOMi HOG000089941.
    HOVERGENi HBG057998.
    KOi K09367.
    OMAi LENMFLQ.
    OrthoDBi EOG7DJSMG.
    TreeFami TF316413.

    Enzyme and pathway databases

    Reactomei REACT_205627. Transcriptional regulation of pluripotent stem cells.
    REACT_216518. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the swine major histocompatibility complex region containing all non-classical class I genes."
      Chardon P., Rogel-Gaillard C., Cattolico L., Duprat S., Vaiman M., Renard C.
      Tissue Antigens 57:55-65(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Large white.
      Tissue: Fibroblast.
    2. "Induced pluripotent stem cells from swine (Sus scrofa): why they may prove to be important."
      Roberts R.M., Telugu B.P., Ezashi T.
      Cell Cycle 8:3078-3081(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY, FUNCTION.

    Entry informationi

    Entry nameiPO5F1_PIG
    AccessioniPrimary (citable) accession number: Q9TSV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3