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Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157DNABy similarity1
Binding sitei164DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi230 – 289HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007511 – 360POU domain, class 5, transcription factor 1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine; by MAPKBy similarity1
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei235PhosphothreonineBy similarity1
Modified residuei236PhosphoserineBy similarity1
Modified residuei289PhosphoserineBy similarity1
Modified residuei290PhosphoserineBy similarity1
Modified residuei355PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).By similarity
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9TSV5
PRIDEiQ9TSV5

Expressioni

Gene expression databases

BgeeiENSSSCG00000001393
GenevisibleiQ9TSV5 SS

Interactioni

Subunit structurei

Interacts with PKM. Interacts with WWP2. Interacts with UBE2I and ZSCAN10. Interacts with PCGF1. Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent. Interacts with ZNF322. Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355. Interacts (when phosphorylated on Ser-355) with FBXW8. Interacts with FBXW4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000001474

Structurei

3D structure databases

ProteinModelPortaliQ9TSV5
SMRiQ9TSV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 212POU-specificPROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 186DNA bindingBy similarity7
Regioni193 – 196DNA bindingBy similarity4

Domaini

The POU-specific domain mediates interaction with PKM.By similarity

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
HOGENOMiHOG000089941
HOVERGENiHBG057998
InParanoidiQ9TSV5
KOiK09367
OMAiLENMFLQ
OrthoDBiEOG091G0U0S
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR015585 POU_dom_5
PANTHERiPTHR11636:SF86 PTHR11636:SF86, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9TSV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGSGIGPG
60 70 80 90 100
VGPGAEVWGL PACPPPYDFC GGMAYCAPQV GVGLVPQGGL ETPQPEGEAG
110 120 130 140 150
AGVESNSEGA SPEPCAAPAG AAKLDKEKLE PNPEESQDIK ALQKDLEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA
310 320 330 340 350
AGSPFPGGPV SFPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEAFPSVSV
360
TPLGSPMHSN
Length:360
Mass (Da):38,342
Last modified:May 1, 2000 - v1
Checksum:i9BEC167A66409A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251914 Genomic DNA Translation: CAB63862.1
RefSeqiNP_001106531.1, NM_001113060.1
UniGeneiSsc.29069

Genome annotation databases

GeneIDi100127461
KEGGissc:100127461

Similar proteinsi

Entry informationi

Entry nameiPO5F1_PIG
AccessioniPrimary (citable) accession number: Q9TSV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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