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Q9TSV5

- PO5F1_PIG

UniProt

Q9TSV5 - PO5F1_PIG

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Protein
POU domain, class 5, transcription factor 1
Gene
POU5F1, OCT3, OCT4
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi230 – 28960Homeobox
Add
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. sequence-specific DNA binding Source: Ensembl
  3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: Ensembl
  4. transcription corepressor activity Source: Ensembl
  5. transcription regulatory region DNA binding Source: Ensembl

GO - Biological processi

  1. ectodermal cell fate commitment Source: Ensembl
  2. endodermal cell fate commitment Source: Ensembl
  3. germ-line stem cell maintenance Source: Ensembl
  4. mRNA transcription from RNA polymerase II promoter Source: Ensembl
  5. mesodermal cell fate commitment Source: Ensembl
  6. negative regulation of calcium ion-dependent exocytosis Source: Ensembl
  7. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  8. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  9. regulation of asymmetric cell division Source: Ensembl
  10. response to retinoic acid Source: Ensembl
  11. trophectodermal cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_205627. Transcriptional regulation of pluripotent stem cells.
REACT_216518. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 7

Subcellular locationi

Cytoplasm. Nucleus
Note: Expressed in a diffuse and slightly punctuate pattern By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleoplasm Source: Ensembl
  3. transcription factor complex Source: Ensembl
  4. transcriptional repressor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360POU domain, class 5, transcription factor 1
PRO_0000100751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by MAPK By similarity
Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei235 – 2351Phosphothreonine By similarity
Modified residuei236 – 2361Phosphoserine By similarity
Modified residuei289 – 2891Phosphoserine By similarity
Modified residuei290 – 2901Phosphoserine By similarity
Modified residuei355 – 3551Phosphoserine By similarity

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation By similarity.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9TSV5.
SMRiQ9TSV5. Positions 144-289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 21275POU-specific
Add
BLAST

Domaini

The POU-specific domain mediates interaction with PKM By similarity.

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
GeneTreeiENSGT00750000117482.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
KOiK09367.
OMAiLENMFLQ.
OrthoDBiEOG7DJSMG.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TSV5-1 [UniParc]FASTAAdd to Basket

« Hide

MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGSGIGPG    50
VGPGAEVWGL PACPPPYDFC GGMAYCAPQV GVGLVPQGGL ETPQPEGEAG 100
AGVESNSEGA SPEPCAAPAG AAKLDKEKLE PNPEESQDIK ALQKDLEQFA 150
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL 200
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL 250
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA 300
AGSPFPGGPV SFPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEAFPSVSV 350
TPLGSPMHSN 360
Length:360
Mass (Da):38,342
Last modified:May 1, 2000 - v1
Checksum:i9BEC167A66409A9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ251914 Genomic DNA. Translation: CAB63862.1.
RefSeqiNP_001106531.1. NM_001113060.1.
UniGeneiSsc.29069.

Genome annotation databases

EnsembliENSSSCT00000001516; ENSSSCP00000001474; ENSSSCG00000001393.
GeneIDi100127461.
KEGGissc:100127461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ251914 Genomic DNA. Translation: CAB63862.1 .
RefSeqi NP_001106531.1. NM_001113060.1.
UniGenei Ssc.29069.

3D structure databases

ProteinModelPortali Q9TSV5.
SMRi Q9TSV5. Positions 144-289.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000001516 ; ENSSSCP00000001474 ; ENSSSCG00000001393 .
GeneIDi 100127461.
KEGGi ssc:100127461.

Organism-specific databases

CTDi 5460.

Phylogenomic databases

eggNOGi NOG329627.
GeneTreei ENSGT00750000117482.
HOGENOMi HOG000089941.
HOVERGENi HBG057998.
KOi K09367.
OMAi LENMFLQ.
OrthoDBi EOG7DJSMG.
TreeFami TF316413.

Enzyme and pathway databases

Reactomei REACT_205627. Transcriptional regulation of pluripotent stem cells.
REACT_216518. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the swine major histocompatibility complex region containing all non-classical class I genes."
    Chardon P., Rogel-Gaillard C., Cattolico L., Duprat S., Vaiman M., Renard C.
    Tissue Antigens 57:55-65(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Large white.
    Tissue: Fibroblast.
  2. "Induced pluripotent stem cells from swine (Sus scrofa): why they may prove to be important."
    Roberts R.M., Telugu B.P., Ezashi T.
    Cell Cycle 8:3078-3081(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY, FUNCTION.

Entry informationi

Entry nameiPO5F1_PIG
AccessioniPrimary (citable) accession number: Q9TSV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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