Q9TRZ7 (TIMP2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metalloproteinase inhibitor 2

Alternative name(s):
Tissue inhibitor of metalloproteinases 2
Short name=TIMP-2

Gene names

Name:

TIMP2

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Subunit structure	Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity By similarity.
Subcellular location	Secreted.
Post-translational modification	The activity of TIMP2 is dependent on the presence of disulfide bonds By similarity.
Sequence similarities	Belongs to the protease inhibitor I35 (TIMP) family. [View classification] Contains 1 NTR domain.

Ontologies

Keywords
Cellular component	Secreted
Ligand	Metal-binding Zinc
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor Protease inhibitor
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Metalloproteinase inhibitor 2		PRO_0000220984
Regions
	Domain	1 – 126	126	NTR
	Region	1 – 5	5	Involved in metalloproteinase-binding By similarity
	Region	69 – 70	2	Involved in metalloproteinase-binding By similarity
Sites
	Metal binding	1	1	Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity
Amino acid modifications
	Disulfide bond	1 ↔ 72		By similarity
	Disulfide bond	3 ↔ 101		By similarity
	Disulfide bond	13 ↔ 126		By similarity
	Disulfide bond	128 ↔ 175		By similarity
	Disulfide bond	133 ↔ 138		By similarity
	Disulfide bond	146 ↔ 167		By similarity
Experimental info
	Sequence conflict	17	1	I → V in AAC95005. Ref.2
	Sequence conflict	25 – 26	2	NK → SE in AAC95005. Ref.2
	Sequence conflict	58	1	Q → K in AAC95005. Ref.2
	Sequence conflict	78	1	I → V in AAC95005. Ref.2
	Sequence conflict	93 – 95	3	NGN → DGR in AAC95005. Ref.2
	Sequence conflict	109	1	T → S in AAC95005. Ref.2
	Sequence conflict	112	1	A → S in AAC95005. Ref.2
	Sequence conflict	131	1	T → S in AAC95005. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9TRZ7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CDC8101A2D38C4A9	FASTA	194	21,849
	10 20 30 40 50 60 CSCSPVHPQQ AFCNADIVIR AKAVNKKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPDQDI 70 80 90 100 110 120 EFIYTAPSSA VCGVSLDIGG KKEYLIAGKA EGNGNMHITL CDFIVPWDTL SATQKKSLNH 130 140 150 160 170 180 RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINRH QAKFFACIKR SDGSCAWYRG 190 AAPPKQEFLD IEDP

References

[1]	"Molecular cloning and characterization of rabbit TIMP2." Wertheimer S.J., Katz S.L. Inflamm. Res. 44:S121-S122(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Temporal alterations in mRNA levels for proteinases and inhibitors and their potential regulators in the healing medial collateral ligament." Reno C., Boykiw R., Martinez M.L., Hart D.A. Biochem. Biophys. Res. Commun. 252:757-763(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-154. Strain: New Zealand white.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069713 mRNA. Translation: AAC95005.1.
UniGene	Ocu.2314.
3D structure databases
ProteinModelPortal	Q9TRZ7.
SMR	Q9TRZ7. Positions 1-192.
ModBase	Search...
Protein family/group databases
MEROPS	I35.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG243625.
HOGENOM	HOG000285981.
HOVERGEN	HBG068749.
OrthoDB	EOG4XPQGS.
Family and domain databases
InterPro	IPR001134. Netrin_domain. IPR001820. Prot_inh_TIMP. IPR008993. TIMP-like_OB-fold. IPR015613. TIMP2. [Graphical view]
PANTHER	PTHR11844. PTHR11844. 1 hit. PTHR11844:SF7. PTHR11844:SF7. 1 hit.
Pfam	PF00965. TIMP. 1 hit. [Graphical view]
SMART	SM00206. NTR. 1 hit. [Graphical view]
SUPFAM	SSF50242. TIMP_like. 1 hit.
PROSITE	PS50189. NTR. 1 hit. PS00288. TIMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIMP2_RABIT

Accession

Primary (citable) accession number: Q9TRZ7
Secondary accession number(s): O97589

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program