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Q9TRY9 (BGAL_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase
EC=3.2.1.23
Alternative name(s):
Acid beta-galactosidase
Short name=Lactase
Gene names
Name:GLB1
OrganismCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Lysosome.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Cellular componentLysosome
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-galactosidase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 295 By similarity
PRO_0000012181
Chain30 – 668639Beta-galactosidase
PRO_0000012182

Sites

Active site1891Proton donor Potential
Active site2691Nucleophile Potential

Amino acid modifications

Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Glycosylation5461N-linked (GlcNAc...) Potential
Glycosylation5561N-linked (GlcNAc...) Potential

Natural variations

Natural variant2801Q → P.
Natural variant4421D → V.
Natural variant4441A → V.

Experimental info

Sequence conflict601R → H Ref.3
Sequence conflict2271K → L Ref.3
Sequence conflict3421G → A Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9TRY9 [UniParc].

Last modified May 2, 2006. Version 3.
Checksum: 56B4A4AC7357221E

FASTA66874,992
        10         20         30         40         50         60 
MARPAAVRVL WALLLPLLLG SARGLRNASQ RTFTIDYSHN RFLKDGQPFR YISGSIHYSR 

        70         80         90        100        110        120 
VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS GEQDVEYFIK LAHELGLLVI 

       130        140        150        160        170        180 
LRPGPYICAE WDMGGLPAWL LLKESIILRS SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP 

       190        200        210        220        230        240 
IITMQVENEY GSYFTCDYDY LRFLQKLFHH HLGNDVLLFT TDGANEKFLQ CGALQGLYAT 

       250        260        270        280        290        300 
VDFGPGANIT AAFQIQRKSE PKGPLVNSEF YTGWLDHWGQ PHSTVRTEVV ASSLHDILAH 

       310        320        330        340        350        360 
GANVNLYMFI GGTNFAYWNG ANMPYQAQPT SYDYDAPLSE AGDLTEKYFA LREVIRKFEK 

       370        380        390        400        410        420 
VPEGFIPPST PKFAYGKVAL KKLKTVEEAL NVLCPPGPIN SLYPLTFIQV KQYFGFVMYR 

       430        440        450        460        470        480 
TTLPQDCSDP TPLSSPLSGV HDRAYVSVDG VPQGVMERSN VITLNITGKA GATLDLLVEN 

       490        500        510        520        530        540 
MGRVNYGRYI NDFKGLISNL TLGSSILTNW MIFPLNTEDA VRSHLGGWHG PNNGRHDKTF 

       550        560        570        580        590        600 
AHRSSNYTLP AFYMGNFSIP SGIPDLPQDT FIQFPGWTKG QVWINGFNLG RYWPARGPQM 

       610        620        630        640        650        660 
TLFVPRHILV TSTPNTIMVL ELEHAPCGDS GPEVCTVEFV DRPVIGAPPT PGHPPPDLSH 


RDLRLDYV

« Hide

References

[1]"The entire coding region of the canine lysosomal beta-galactosidase (GLB1) gene."
Kreutzer R., Mueller G., Leeb T., Moritz A., Baumgaertner W.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skin.
[2]"A partial sequence of canine lysosomal beta-galactosidase (GLB1)."
Smith B.F., Georgeson M., Baker H.J.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-668.
Strain: Beagle.
Tissue: Brain.
[3]"Comparison of the canine and human acid beta-galactosidase gene."
Ahern-Rindell A.J., Kretz K.A., O'Brien J.S.
Am. J. Med. Genet. 63:340-345(1996) [PubMed: 8725782] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-668.
Tissue: Kidney, Muscle, Pancreas and Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		DQ196436 mRNA. Translation: ABA43388.1.
AF056084 mRNA. Translation: AAC12775.1.
RefSeqNP_001032730.1. NM_001037641.1.
UniGeneCfa.22721.

3D structure databases

ProteinModelPortalQ9TRY9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9TRY9.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID403873.
KEGGcfa:403873.

Organism-specific databases

CTD2720.

Phylogenomic databases

eggNOGmaNOG10463.
GeneTreeENSGT00390000006586.
HOVERGENHBG004841.
InParanoidQ9TRY9.
OrthoDBEOG46MBJ7.

Family and domain databases

InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK12309.
PANTHERPTHR23421. Glyco_hydro_35. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_CANFA
AccessionPrimary (citable) accession number: Q9TRY9
Secondary accession number(s): O62800, Q3HTI1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 2, 2006
Last modified: November 16, 2011
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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