Reviewed, UniProtKB/Swiss-Prot Q9TRY9 (BGAL_CANFA)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
    EC=3.2.1.23
Alternative name(s):
    Acid beta-galactosidase
      Short name=Lactase

Gene names

Name:

GLB1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 29	5	By similarity		PRO_0000012181
	Chain	30 – 668	639	Beta-galactosidase		PRO_0000012182
Sites
	Active site	189	1	Proton donor Potential
	Active site	269	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	499	1	N-linked (GlcNAc...) Potential
	Glycosylation	546	1	N-linked (GlcNAc...) Potential
	Glycosylation	556	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	280	1	Q → P
	Natural variant	442	1	D → V
	Natural variant	444	1	A → V
Experimental info
	Sequence conflict	60	1	R → H Ref.3
	Sequence conflict	227	1	K → L Ref.3
	Sequence conflict	342	1	G → A Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9TRY9-1 [UniParc]. Last modified May 2, 2006. Version 3. Checksum: 56B4A4AC7357221E	FASTA	668	74,992
	10 20 30 40 50 60 MARPAAVRVL WALLLPLLLG SARGLRNASQ RTFTIDYSHN RFLKDGQPFR YISGSIHYSR 70 80 90 100 110 120 VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS GEQDVEYFIK LAHELGLLVI 130 140 150 160 170 180 LRPGPYICAE WDMGGLPAWL LLKESIILRS SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP 190 200 210 220 230 240 IITMQVENEY GSYFTCDYDY LRFLQKLFHH HLGNDVLLFT TDGANEKFLQ CGALQGLYAT 250 260 270 280 290 300 VDFGPGANIT AAFQIQRKSE PKGPLVNSEF YTGWLDHWGQ PHSTVRTEVV ASSLHDILAH 310 320 330 340 350 360 GANVNLYMFI GGTNFAYWNG ANMPYQAQPT SYDYDAPLSE AGDLTEKYFA LREVIRKFEK 370 380 390 400 410 420 VPEGFIPPST PKFAYGKVAL KKLKTVEEAL NVLCPPGPIN SLYPLTFIQV KQYFGFVMYR 430 440 450 460 470 480 TTLPQDCSDP TPLSSPLSGV HDRAYVSVDG VPQGVMERSN VITLNITGKA GATLDLLVEN 490 500 510 520 530 540 MGRVNYGRYI NDFKGLISNL TLGSSILTNW MIFPLNTEDA VRSHLGGWHG PNNGRHDKTF 550 560 570 580 590 600 AHRSSNYTLP AFYMGNFSIP SGIPDLPQDT FIQFPGWTKG QVWINGFNLG RYWPARGPQM 610 620 630 640 650 660 TLFVPRHILV TSTPNTIMVL ELEHAPCGDS GPEVCTVEFV DRPVIGAPPT PGHPPPDLSH RDLRLDYV

References

[1]	"The entire coding region of the canine lysosomal beta-galactosidase (GLB1) gene." Kreutzer R., Mueller G., Leeb T., Moritz A., Baumgaertner W. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[2]	"A partial sequence of canine lysosomal beta-galactosidase (GLB1)." Smith B.F., Georgeson M., Baker H.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-668. Strain: Beagle. Tissue: Brain.
[3]	"Comparison of the canine and human acid beta-galactosidase gene." Ahern-Rindell A.J., Kretz K.A., O'Brien J.S. Am. J. Med. Genet. 63:340-345(1996) [PubMed: 8725782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-668. Tissue: Kidney, Muscle, Pancreas and Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ196436 mRNA. Translation: ABA43388.1. AF056084 mRNA. Translation: AAC12775.1.
RefSeq	NP_001032730.1.
UniGene	Cfa.22721
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9TRY9.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Genome annotation databases
Ensembl	ENSCAFT00000007487; ENSCAFP00000006929; ENSCAFG00000004652; Canis familiaris. [Genome view]
GeneID	403873.
KEGG	cfa:403873.
Organism-specific databases
CTD	403873.
Phylogenomic databases
HOVERGEN	Q9TRY9.
Enzyme and pathway databases
BRENDA	3.2.1.23. 463.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_CANFA

Accession

Primary (citable) accession number: Q9TRY9
Secondary accession number(s): O62800, Q3HTI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	May 2, 2006
Last modified:	September 1, 2009
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)