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Protein

Beta-galactosidase

Gene

GLB1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei188SubstrateBy similarity1
Active sitei189Proton donorBy similarity1
Active sitei269NucleophileBy similarity1
Binding sitei334SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.231 Publication)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Gene namesi
Name:GLB1
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000001218125 – 29By similarity5
ChainiPRO_000001218230 – 668Beta-galactosidaseAdd BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi196 ↔ 231By similarity
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi465N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi499N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi556N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi627 ↔ 635By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9TRY9
PRIDEiQ9TRY9

Interactioni

Subunit structurei

Homodimer. May form higher multimers.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000006929

Structurei

3D structure databases

ProteinModelPortaliQ9TRY9
SMRiQ9TRY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496 Eukaryota
COG1874 LUCA
HOGENOMiHOG000221607
HOVERGENiHBG004841
InParanoidiQ9TRY9
KOiK12309

Family and domain databases

Gene3Di2.60.120.260, 3 hits
InterProiView protein in InterPro
IPR026283 B-gal_1-like
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF13364 BetaGal_dom4_5, 1 hit
PF01301 Glyco_hydro_35, 1 hit
PIRSFiPIRSF006336 B-gal, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SUPFAMiSSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9TRY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPAAVRVL WALLLPLLLG SARGLRNASQ RTFTIDYSHN RFLKDGQPFR
60 70 80 90 100
YISGSIHYSR VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS
110 120 130 140 150
GEQDVEYFIK LAHELGLLVI LRPGPYICAE WDMGGLPAWL LLKESIILRS
160 170 180 190 200
SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP IITMQVENEY GSYFTCDYDY
210 220 230 240 250
LRFLQKLFHH HLGNDVLLFT TDGANEKFLQ CGALQGLYAT VDFGPGANIT
260 270 280 290 300
AAFQIQRKSE PKGPLVNSEF YTGWLDHWGQ PHSTVRTEVV ASSLHDILAH
310 320 330 340 350
GANVNLYMFI GGTNFAYWNG ANMPYQAQPT SYDYDAPLSE AGDLTEKYFA
360 370 380 390 400
LREVIRKFEK VPEGFIPPST PKFAYGKVAL KKLKTVEEAL NVLCPPGPIN
410 420 430 440 450
SLYPLTFIQV KQYFGFVMYR TTLPQDCSDP TPLSSPLSGV HDRAYVSVDG
460 470 480 490 500
VPQGVMERSN VITLNITGKA GATLDLLVEN MGRVNYGRYI NDFKGLISNL
510 520 530 540 550
TLGSSILTNW MIFPLNTEDA VRSHLGGWHG PNNGRHDKTF AHRSSNYTLP
560 570 580 590 600
AFYMGNFSIP SGIPDLPQDT FIQFPGWTKG QVWINGFNLG RYWPARGPQM
610 620 630 640 650
TLFVPRHILV TSTPNTIMVL ELEHAPCGDS GPEVCTVEFV DRPVIGAPPT
660
PGHPPPDLSH RDLRLDYV
Length:668
Mass (Da):74,992
Last modified:May 2, 2006 - v3
Checksum:i56B4A4AC7357221E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60R → H (PubMed:8725782).Curated1
Sequence conflicti227K → L (PubMed:8725782).Curated1
Sequence conflicti342G → A (PubMed:8725782).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti280Q → P. 1
Natural varianti442D → V. 1
Natural varianti444A → V. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ196436 mRNA Translation: ABA43388.1
AF056084 mRNA Translation: AAC12775.1
RefSeqiNP_001032730.1, NM_001037641.1
UniGeneiCfa.22721

Genome annotation databases

GeneIDi403873
KEGGicfa:403873

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiBGAL_CANLF
AccessioniPrimary (citable) accession number: Q9TRY9
Secondary accession number(s): O62800, Q3HTI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 2, 2006
Last modified: May 23, 2018
This is version 100 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health