Q9TM10 (MENB_CYACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-Dihydroxy-2-naphthoyl-CoA synthase
Short name=DHNA-CoA synthase
EC=4.1.3.36

Gene names

Name:

menB

Encoded on

Plastid; Chloroplast

Organism

Cyanidium caldarium

Taxonomic identifier

2771 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidium

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).
Catalytic activity	4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H2O.
Cofactor	Bicarbonate ion. The bicarbonate anion plays the same catalytic role (proton acceptor) as the side-chain carboxylate group of the essential 'Asp-185' found in actinobacteria, archaea, bacteroidetes, and deltaproteobacteria By similarity.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 6/8.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Cellular component	Chloroplast Plastid
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1,4-dihydroxy-2-naphthoyl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	1,4-Dihydroxy-2-naphthoyl-CoA synthase		PRO_0000109329
Regions
	Region	30 – 31	2	Substrate binding By similarity
	Region	70 – 74	5	Substrate binding By similarity
	Region	114 – 118	5	Substrate binding By similarity
	Region	139 – 141	3	Bicarbonate binding By similarity
Sites
	Binding site	140	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Site	148	1	Important for catalysis Potential
	Site	243	1	Important for catalysis Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9TM10 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2A18ACEDA4A1F15F	FASTA	268	29,750
	10 20 30 40 50 60 MQTIAIKDFI DIKYIKQDQI SEIIISRPQV LNAFRPRTIN EIIAAFYDSR EDSSIGVVIL 70 80 90 100 110 120 SGHGSRAFCV GGDQKIRSKT GYIDEKGRSS LNVLELQRII RTFPKPVIAK VSGYAVGGGQ 130 140 150 160 170 180 ILNMMCDLTI ASENAVLGQS GPKVGSFDAG YGSAYMARII GQKKARELWF TCYQYDAFEA 190 200 210 220 230 240 YKMGLVNWVV KIEDLDSYAI KLATDILNKS PLAIRFLKSS LNADCDGQSG LQELAGYSTM 250 260 LFYMSAEGQE GHRAFLENRE PDFSKFNS

References

[1]	"The structure and gene repertoire of an ancient red algal plastid genome." Gloeckner G., Rosenthal A., Valentin K.-U. J. Mol. Evol. 51:382-390(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RK-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022186 Genomic DNA. Translation: AAF12988.1.
RefSeq	NP_045107.1. NC_001840.1.
3D structure databases
ProteinModelPortal	Q9TM10.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	800186.
Phylogenomic databases
ProtClustDB	CLSC624827.
Enzyme and pathway databases
UniPathway	UPA00079; UER00167.
Family and domain databases
Gene3D	1.10.12.10. 1 hit.
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core_superfam. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR010198. Naphthoate_synthase. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR01929. menB. 1 hit.
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MENB_CYACA

Accession

Primary (citable) accession number: Q9TM10

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)