Reviewed, UniProtKB/Swiss-Prot Q9TLR8 (ACSF_CYACA)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Synonyms:	ycf59

Encoded on

Plastid; Chloroplast

Organism

Cyanidium caldarium

Taxonomic identifier

2771 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Subcellular location	Plastid › chloroplast. HAMAP MF_01840
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000217541

Sequences

	Sequence		Length	Mass (Da)
	Q9TLR8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E25EE64E12E045DA	FASTA	354	42,732
	10 20 30 40 50 60 MVLNANHLQT KKTIKETLLA PRFYTTDFDE ISRYDISSNF EEIEAIVNEF RSDYNKKHFI 70 80 90 100 110 120 RDEEFDRHWS QIDAQTKQMF VEFLERSCTA EFSGFLLYKE LARKLKEKMP VLAEGFLLMS 130 140 150 160 170 180 RDEARHAGFL NKAMADFNLT LDLGFMTKNR RYTFFKPKFI FYATYLSEKI GYWRYITIYR 190 200 210 220 230 240 HLEKHPEHRI YPIFKFFESW CQDENRHGDF FAAIVKSQPY LLEGFISKLW CRFFLLAVFA 250 260 270 280 290 300 TMYLNDCQRG EFYKSIGLDP RQFDIYVIQK TNESAAKLFP TVLDLDNPRF FECLDKCACY 310 320 330 340 350 NKLLLEIDDR EQPFFNQVLN VFYKFYLYFL LVLNFIMLYF LRTVNCNSFT RMVK

References

[1]	"The structure and gene repertoire of an ancient red algal plastid genome." Gloeckner G., Rosenthal A., Valentin K.-U. J. Mol. Evol. 51:382-390(2000) [PubMed: 11040290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RK-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022186 Genomic DNA. Translation: AAF12893.1.
RefSeq	NP_045201.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	800267.
Enzyme and pathway databases
BRENDA	1.14.13.81. 145.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_CYACA

Accession

Primary (citable) accession number: Q9TLR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)