Reviewed, UniProtKB/Swiss-Prot Q9THX6 (APX4_SOLLC)

Names and origin

Protein names

Recommended name:
    Putative L-ascorbate peroxidase, chloroplastic
    EC=1.11.1.11
Alternative name(s):
    Thylakoid lumenal 29 kDa protein
      Short name=TL29
    P29
    LeAPx09

Gene names

Name:

CLEB3J9

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a key role in hydrogen peroxide removal By similarity.
Catalytic activity	L-ascorbate + H2O2 = dehydroascorbate + 2 H2O.
Subcellular location	Plastid › chloroplast thylakoid lumen. Ref.1
Sequence similarities	Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
Caution	Lacks the heme-binding site, the proton acceptor and the transition state stabilizer, which are conserved features of the ascorbate peroxidase.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Chloroplast Plastid Thylakoid
Domain	Transit peptide
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Transit peptide	? – 78		Thylakoid By similarity
	Chain	79 – 345	267	Putative L-ascorbate peroxidase, chloroplastic		PRO_0000023636

Sequences

	Sequence		Length	Mass (Da)
	Q9THX6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 681CAEBFDDBEB41B	FASTA	345	37,750
	10 20 30 40 50 60 MVSFASTLPS LVSFIPSPSS ITNASRNPPQ PGMICCKFRS ELNNEDRFHR RDILQSVGAA 70 80 90 100 110 120 VGMDLIARSS AFIEVANAAD LIQRRQRSDF QSKIKLTLYD AIKANPDIIP SLLTLALNDA 130 140 150 160 170 180 ITYDKATKTG GPNGSIRFSS EISRPENKGL DAALNLLEES KKVIDLDSKG GPISYADLIQ 190 200 210 220 230 240 FAAQSAVKST FIASAISKCG GNVEKGTLLY SAYGSNGQWG QFDRIFGRSD AQEPDPEGRV 250 260 270 280 290 300 PQWDKASVQE MKDKFKAVGL GPRQLAVMSS FLGPDQAATE ALLASDPEVL PWIQKYQRSR 310 320 330 340 ETVSRTDYEV DLITTVTKLS SLGQVINYEA YTYPPRKIDV TKLKL

References

[1]

"A peroxidase homologue and novel plastocyanin located by proteomics to the Arabidopsis chloroplast thylakoid lumen." Kieselbach T., Bystedt M., Hynds P., Robinson C., Schroeder W.P. FEBS Lett. 480:271-276(2000) [PubMed: 11034343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ251882 Genomic DNA. Translation: CAB64343.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAF based on UniProtKB Q43758.
ModBase	Search...
Protein family/group databases
PeroxiBase	3921. LeAPx09.
Enzyme and pathway databases
BRENDA	1.11.1.11. 281054.
Family and domain databases
InterPro	IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00459. ASPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX4_SOLLC

Accession

Primary (citable) accession number: Q9THX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)