Reviewed, UniProtKB/Swiss-Prot Q9TC96 (NDUS2_NEPOL)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH dehydrogenase subunit 7

Gene names

Name:

NAD7

Encoded on

Mitochondrion

Organism

Nephroselmis olivacea

Taxonomic identifier

31312 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Prasinophyceae › Pseudoscourfieldiales › Pycnococcaceae › Nephroselmis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	NADH-ubiquinone oxidoreductase 49 kDa subunit		PRO_0000118584
Sites
	Metal binding	261	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	267	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	282	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9TC96-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BCBD8FE4AACB08EB	FASTA	398	45,476
	10 20 30 40 50 60 MAIEHASSIK KVKNFTLNFG PQHPAAHGVL RLVLELNGEV VARADPHIGL LHRGTEKLIE 70 80 90 100 110 120 YKTYTQALPY FDRLDYVSMM CQEHAYSLAV EKLLHCEVPE RAQYIRVLFS EITRILNHLL 130 140 150 160 170 180 ALTTHAMDVG ALTPFLWAFE EREKLIEFYE RVSGSRMHAA YIRPGGVACD LPANLCEDIY 190 200 210 220 230 240 LFCQQFASRI DEMEEMLTNN RIWKQRLVDI GIVTAENAFA WGFSGVLLRG SGVAWDLRKT 250 260 270 280 290 300 QPYDVYNRMI FDVPVGTQGD CYDRYLCRVE EMRQSIHIIM QCLNQLPKGM IKADDKKITP 310 320 330 340 350 360 PSRSQMKQSM ESLIHHFKLF TEGYTVPNSE TYTSVEAPKG EFGVYLVSNG TNRPYRCKIR 370 380 390 APGFLHLQGL DMMSKNHMLA DVVTIIGTQD IVFGEVDR

References

[1]

"The complete mitochondrial DNA sequences of Nephroselmis olivacea and Pedinomonas minor: two radically different evolutionary patterns within the green algae." Turmel M., Lemieux C., Burger G., Lang B.F., Otis C., Plante I., Gray M.W. Plant Cell 11:1717-1730(1999) [PubMed: 10488238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NIES-484.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF110138 Genomic DNA. Translation: AAF03201.1.
RefSeq	YP_665674.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4178038.
Enzyme and pathway databases
BRENDA	1.6.5.3. 279958. 1.6.99.3. 279958.
Family and domain databases
InterPro	IPR010219. NADH_DH_1_dsu. IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_NEPOL

Accession

Primary (citable) accession number: Q9TC96

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)