Q9T6H8 (CCF1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cytochrome c biogenesis ccmF N-terminal-like mitochondrial protein

Gene names

Name:	CCMFN1
Synonyms:	CC6BN1, CCB382
Ordered Locus Names:	AtMg00830

Encoded on

Mitochondrion

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the export of heme to the mitochondrion for the biogenesis of c-type cytochromes By similarity.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Potential.
Miscellaneous	A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07783) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.
Sequence similarities	Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family.
RNA editing	Edited at positions 15, 35, 48, 53, 67, 88, 90, 97, 114, 124, 135, 162, 194, 237, 240, 260, 264, 269 and 319. Ref.5
Sequence caution	The sequence CAA66908.1 differs from that shown. Reason: Its N-terminal part is produced after trans-splicing. No experimental data confirms this assertion.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Membrane Mitochondrion
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain complex IV assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCMFN2	Q33884	5	EBI-1797466,EBI-763400

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Putative cytochrome c biogenesis ccmF N-terminal-like mitochondrial protein		PRO_0000201594
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9T6H8 [UniParc]. Last modified March 29, 2004. Version 3. Checksum: 7B9100599199E244	FASTA	382	43,647
	10 20 30 40 50 60 MSISIYEFFH YSLFLGLFVA FTYNKKQPPA FGAALAFWCI LLSFLGLLFC HISNNLSNYN 70 80 90 100 110 120 VLTANALFFY QISGTWSNHE GSILLWCWIL NFYGFFFCYR GRPQSHNVLK QGGYRESLFF 130 140 150 160 170 180 FFVLNFVKNS ILSLLRYEQE SGLKNQLYTP FVLRTLVDSE LCSRRNRTFD GPALFYAPLY 190 200 210 220 230 240 PERKIKNPLD AWRFRGSREG KRTHPLLHLA RDDKERASSI DEQRIDGALG IALFFSFFLL 250 260 270 280 290 300 ASSDPFVRNF FVCTEPLAEL NPVLQDPILA IHPPCIYAGD VASAEGFGLC RSKMMNGIVA 310 320 330 340 350 360 LHSPPMRKDA AEKNGTLLCS AGCVGSRITS ELFTLKFKHV GAKCYPALLL RSNRSPLMLL 370 380 RRRFFAFSSF WAGARSHSTK RY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schuster W. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. C24.
[2]	"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides." Unseld M., Marienfeld J.R., Brandt P., Brennicke A. Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07783). Strain: cv. Columbia.
[4]	"Genetic architecture of mitochondrial editing in Arabidopsis thaliana." Bentolila S., Elliott L.E., Hanson M.R. Genetics 178:1693-1708(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-184, RNA-EDITING. Strain: cv. Columbia and cv. Landsberg erecta. Tissue: Rosette leaf.
[5]	"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs." Giege P., Brennicke A. Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98254 Genomic DNA. Translation: CAA66908.1. Sequence problems. Y08501 Genomic DNA. Translation: CAA69825.3. Sequence problems. AC007143 Genomic DNA. No translation available. AC007730 Genomic DNA. No translation available. EF488898 mRNA. Translation: ABS50610.1. EF488899 mRNA. Translation: ABS50611.1.
IPI	IPI00527545.
PIR	S71281.
RefSeq	NP_085539.2. NC_001284.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9T6H8. 3 interactions.
Proteomic databases
PaxDb	Q9T6H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3890472.
KEGG	ath:ArthMp072.
Organism-specific databases
TAIR	AtMg00830.
Phylogenomic databases
eggNOG	NOG259132.
HOGENOM	HOG000176967.
Gene expression databases
Genevestigator	Q9T6H8.
GermOnline	ATMG00830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002541. Cyt_c_assembly. IPR003567. Cyt_c_biogenesis. IPR003569. Cyt_c_biogenesis_CcbS. [Graphical view]
Pfam	PF01578. Cytochrom_C_asm. 2 hits. [Graphical view]
PRINTS	PR01410. CCBIOGENESIS. PR01412. CCBSBIOGNSIS.
ProtoNet	Search...

Entry information

Entry name

CCF1_ARATH

Accession

Primary (citable) accession number: Q9T6H8
Secondary accession number(s): A7KNF5 , O03601, Q1ZXZ2, Q31706

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	May 1, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program