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Protein

Endolysin

Gene

lys

Organism
Escherichia phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity.By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei37Proton donorBy similarity1
Active sitei46NucleophileBy similarity1
Active sitei52By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Gene product 22
Short name:
gp22
Lysis protein
Lysozyme
Muramidase
Gene namesi
Name:lys
Ordered Locus Names:Mup22
OrganismiEscherichia phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMuvirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180961 – 171EndolysinAdd BLAST171

Expressioni

Inductioni

Expressed in the intermediate phase of the viral replicative cycle.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9T1X2.
SMRiQ9T1X2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Phylogenomic databases

KOiK01185.
OrthoDBiVOG090000CE.

Family and domain databases

CDDicd00737. endolysin_autolysin. 1 hit.
Gene3Di1.10.530.40. 1 hit.
InterProiView protein in InterPro
IPR033907. Endolysin_autolysin.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
PfamiView protein in Pfam
PF00959. Phage_lysozyme. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9T1X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIPKKLKA ALLAVTIAGG GVGGYQEMTR QSLIHLENIA YMPYRDIAGV
60 70 80 90 100
LTVCVGHTGP DIEMRRYSHA ECMALLDSDL KPVYAAIDRL VRVPLTPYQK
110 120 130 140 150
TALATFIFNT GVTAFSKSTL LKKLNAGDYA GARDQMARWV FAAGHKWKGL
160 170
MNRREVEMAI WNIRGADDLR Q
Length:171
Mass (Da):18,884
Last modified:May 1, 2000 - v1
Checksum:i9F53C6ADF47D4789
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01099.1.
RefSeqiNP_050626.1. NC_000929.1.

Genome annotation databases

GeneIDi2636261.
KEGGivg:2636261.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENLYS_BPMU
AccessioniPrimary (citable) accession number: Q9T1X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families