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Q9T1V4

- BP45_BPMU

UniProt

Q9T1V4 - BP45_BPMU

Protein

Baseplate puncturing device gp45

Gene

Mup45

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Component of the baseplate that forms a central needlelike spike used to puncture the host cell membrane for tube insertion during virus entry. Probably involved in baseplate and tail assembly. Serves as the distal plug of tail tube channel and might regulate the process of the phage DNA and protein ejection into the host cell.2 Publications

    Cofactori

    Binds 1 calcium ion per trimer. Calcium ion plays an important role in interaction with the host cell membrane.1 Publication
    Binds 1 chloride ion per trimer.1 Publication
    Binds 1 iron ion per trimer.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi183 – 1831Iron; via tele nitrogen; shared with trimeric partners
    Metal bindingi185 – 1851Iron; via tele nitrogen; shared with trimeric partners
    Metal bindingi188 – 1881Calcium; shared with trimeric partners
    Binding sitei188 – 1881Chloride; via amide nitrogen; shared with trimeric partners
    Metal bindingi189 – 1891Calcium; shared with trimeric partners

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Viral contractile tail ejection system

    Keywords - Biological processi

    Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

    Keywords - Ligandi

    Calcium, Chloride, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Baseplate puncturing device gp45
    Alternative name(s):
    Gene product 45
    Short name:
    gp45
    Gene product Q
    Short name:
    gpQ
    Gene namesi
    Ordered Locus Names:Mup45
    OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
    Taxonomic identifieri10677 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
    Virus hostiEnterobacteriaceae [TaxID: 543]
    ProteomesiUP000002611: Genome

    Subcellular locationi

    Virion. Host cytoplasm Curated
    Note: Baseplate.Curated

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

    Pathology & Biotechi

    Disruption phenotypei

    No tail is synthesized.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi188 – 1881D → A: Loss of membrane-binding ability. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 197197Baseplate puncturing device gp45PRO_0000077838Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homotrimer.2 Publications

    Structurei

    Secondary structure

    1
    197
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi104 – 1074
    Beta strandi113 – 1175
    Turni118 – 1203
    Beta strandi121 – 14020
    Beta strandi142 – 15413
    Beta strandi156 – 1605
    Beta strandi162 – 1665
    Beta strandi168 – 1714
    Beta strandi173 – 1753
    Turni180 – 1823
    Beta strandi190 – 1923

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VTNX-ray1.75A100-197[»]
    3VTOX-ray1.44A/B/C/P/Q/R92-197[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni177 – 19721Ions bindingAdd
    BLAST

    Domaini

    The C-terminus is a needlelike shaped homotrimer consisting of an intertwined beta-sheet, a triple beta-helix and a metal-binding region.1 Publication

    Family and domain databases

    InterProiIPR013046. Phage_Mu/P2_baseplate_assem.
    IPR014462. Phage_Mu_Gp45.
    [Graphical view]
    PfamiPF06890. Phage_Mu_Gp45. 1 hit.
    [Graphical view]
    PIRSFiPIRSF012337. gp45. 1 hit.
    TIGRFAMsiTIGR01644. phage_P2_V. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9T1V4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERVNDSALN RLLTPLMRRV RLMLARAVVN VINDGRKVQN LQVGLLDDEE    50
    SDEVERLQNY GHFSVPLPGA EALIACVGAQ RDQGIAVVVE DRRYRPTNLE 100
    PGDAGIYHHE GHRIRLTKDG RCIITCKTVE VYADESMTVD TPRTTFTGDV 150
    EIQKGLGVKG KSQFDSNITA PDAIINGKST DKHIHRGDSG GTTGPMQ 197
    Length:197
    Mass (Da):21,689
    Last modified:May 1, 2000 - v1
    Checksum:i448C7A7ADCADD5C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083977 Genomic DNA. Translation: AAF01123.1.
    RefSeqiNP_050649.1. NC_000929.1.

    Genome annotation databases

    GeneIDi2636280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083977 Genomic DNA. Translation: AAF01123.1 .
    RefSeqi NP_050649.1. NC_000929.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VTN X-ray 1.75 A 100-197 [» ]
    3VTO X-ray 1.44 A/B/C/P/Q/R 92-197 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2636280.

    Family and domain databases

    InterProi IPR013046. Phage_Mu/P2_baseplate_assem.
    IPR014462. Phage_Mu_Gp45.
    [Graphical view ]
    Pfami PF06890. Phage_Mu_Gp45. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF012337. gp45. 1 hit.
    TIGRFAMsi TIGR01644. phage_P2_V. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
      Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
      J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu."
      Grundy F.J., Howe M.M.
      Virology 143:485-504(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    3. "Mutational analysis of a C-dependent late promoter of bacteriophage Mu."
      Chiang L.W., Howe M.M.
      Genetics 135:619-629(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "The C-terminal domain is sufficient for host-binding activity of the Mu phage tail-spike protein."
      Suzuki H., Yamada S., Toyama Y., Takeda S.
      Biochim. Biophys. Acta 1804:1738-1742(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    5. "Contractile tail machines of bacteriophages."
      Leiman P.G., Shneider M.M.
      Adv. Exp. Med. Biol. 726:93-114(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    6. "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
      Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
      Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.

    Entry informationi

    Entry nameiBP45_BPMU
    AccessioniPrimary (citable) accession number: Q9T1V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    Translation initiates from a non-canonical start codon (GUG).Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3