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Protein

Baseplate puncturing device gp45

Gene

Mup45

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the baseplate that forms a central needlelike spike used to puncture the host cell membrane for tube insertion during virus entry. Probably involved in baseplate and tail assembly. Serves as the distal plug of tail tube channel and might regulate the process of the phage DNA and protein ejection into the host cell.2 Publications

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi183Iron; via tele nitrogen; shared with trimeric partners1
Metal bindingi185Iron; via tele nitrogen; shared with trimeric partners1
Metal bindingi188Calcium; shared with trimeric partners1
Binding sitei188Chloride; via amide nitrogen; shared with trimeric partners1
Metal bindingi189Calcium; shared with trimeric partners1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Viral contractile tail ejection system

Keywords - Biological processi

Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

Keywords - Ligandi

Calcium, Chloride, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate puncturing device gp45
Alternative name(s):
Gene product 45
Short name:
gp45
Gene product Q
Short name:
gpQ
Gene namesi
Ordered Locus Names:Mup45
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechi

Disruption phenotypei

No tail is synthesized.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188D → A: Loss of membrane-binding ability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000778381 – 197Baseplate puncturing device gp45Add BLAST197

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer.2 Publications

Structurei

Secondary structure

1197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi104 – 107Combined sources4
Beta strandi113 – 117Combined sources5
Turni118 – 120Combined sources3
Beta strandi121 – 140Combined sources20
Beta strandi142 – 154Combined sources13
Beta strandi156 – 160Combined sources5
Beta strandi162 – 166Combined sources5
Beta strandi168 – 171Combined sources4
Beta strandi173 – 175Combined sources3
Turni180 – 182Combined sources3
Beta strandi190 – 192Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VTNX-ray1.75A100-197[»]
3VTOX-ray1.44A/B/C/P/Q/R92-197[»]
SMRiQ9T1V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni177 – 197Ions bindingAdd BLAST21

Domaini

The C-terminus is a needlelike shaped homotrimer consisting of an intertwined beta-sheet, a triple beta-helix and a metal-binding region.1 Publication

Family and domain databases

InterProiIPR013046. GpV/Gp45.
IPR014462. Phage_Mu_Gp45.
[Graphical view]
PfamiPF06890. Phage_Mu_Gp45. 1 hit.
[Graphical view]
PIRSFiPIRSF012337. gp45. 1 hit.
TIGRFAMsiTIGR01644. phage_P2_V. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9T1V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERVNDSALN RLLTPLMRRV RLMLARAVVN VINDGRKVQN LQVGLLDDEE
60 70 80 90 100
SDEVERLQNY GHFSVPLPGA EALIACVGAQ RDQGIAVVVE DRRYRPTNLE
110 120 130 140 150
PGDAGIYHHE GHRIRLTKDG RCIITCKTVE VYADESMTVD TPRTTFTGDV
160 170 180 190
EIQKGLGVKG KSQFDSNITA PDAIINGKST DKHIHRGDSG GTTGPMQ
Length:197
Mass (Da):21,689
Last modified:May 1, 2000 - v1
Checksum:i448C7A7ADCADD5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01123.1.
RefSeqiNP_050649.1. NC_000929.1.

Genome annotation databases

GeneIDi2636280.
KEGGivg:2636280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01123.1.
RefSeqiNP_050649.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VTNX-ray1.75A100-197[»]
3VTOX-ray1.44A/B/C/P/Q/R92-197[»]
SMRiQ9T1V4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2636280.
KEGGivg:2636280.

Family and domain databases

InterProiIPR013046. GpV/Gp45.
IPR014462. Phage_Mu_Gp45.
[Graphical view]
PfamiPF06890. Phage_Mu_Gp45. 1 hit.
[Graphical view]
PIRSFiPIRSF012337. gp45. 1 hit.
TIGRFAMsiTIGR01644. phage_P2_V. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBP45_BPMU
AccessioniPrimary (citable) accession number: Q9T1V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Translation initiates from a non-canonical start codon (GUG).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.