Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9T1V4

- BP45_BPMU

UniProt

Q9T1V4 - BP45_BPMU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Baseplate puncturing device gp45

Gene

Mup45

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the baseplate that forms a central needlelike spike used to puncture the host cell membrane for tube insertion during virus entry. Probably involved in baseplate and tail assembly. Serves as the distal plug of tail tube channel and might regulate the process of the phage DNA and protein ejection into the host cell.2 Publications

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi183 – 1831Iron; via tele nitrogen; shared with trimeric partners
Metal bindingi185 – 1851Iron; via tele nitrogen; shared with trimeric partners
Metal bindingi188 – 1881Calcium; shared with trimeric partners
Binding sitei188 – 1881Chloride; via amide nitrogen; shared with trimeric partners
Metal bindingi189 – 1891Calcium; shared with trimeric partners

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Viral contractile tail ejection system

Keywords - Biological processi

Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

Keywords - Ligandi

Calcium, Chloride, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate puncturing device gp45
Alternative name(s):
Gene product 45
Short name:
gp45
Gene product Q
Short name:
gpQ
Gene namesi
Ordered Locus Names:Mup45
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
ProteomesiUP000002611: Genome

Subcellular locationi

Virion. Host cytoplasm Curated
Note: Baseplate.Curated

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechi

Disruption phenotypei

No tail is synthesized.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi188 – 1881D → A: Loss of membrane-binding ability. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Baseplate puncturing device gp45PRO_0000077838Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer.2 Publications

Structurei

Secondary structure

1
197
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi104 – 1074Combined sources
Beta strandi113 – 1175Combined sources
Turni118 – 1203Combined sources
Beta strandi121 – 14020Combined sources
Beta strandi142 – 15413Combined sources
Beta strandi156 – 1605Combined sources
Beta strandi162 – 1665Combined sources
Beta strandi168 – 1714Combined sources
Beta strandi173 – 1753Combined sources
Turni180 – 1823Combined sources
Beta strandi190 – 1923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VTNX-ray1.75A100-197[»]
3VTOX-ray1.44A/B/C/P/Q/R92-197[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni177 – 19721Ions bindingAdd
BLAST

Domaini

The C-terminus is a needlelike shaped homotrimer consisting of an intertwined beta-sheet, a triple beta-helix and a metal-binding region.1 Publication

Family and domain databases

InterProiIPR013046. Phage_Mu/P2_baseplate_assem.
IPR014462. Phage_Mu_Gp45.
[Graphical view]
PfamiPF06890. Phage_Mu_Gp45. 1 hit.
[Graphical view]
PIRSFiPIRSF012337. gp45. 1 hit.
TIGRFAMsiTIGR01644. phage_P2_V. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9T1V4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERVNDSALN RLLTPLMRRV RLMLARAVVN VINDGRKVQN LQVGLLDDEE
60 70 80 90 100
SDEVERLQNY GHFSVPLPGA EALIACVGAQ RDQGIAVVVE DRRYRPTNLE
110 120 130 140 150
PGDAGIYHHE GHRIRLTKDG RCIITCKTVE VYADESMTVD TPRTTFTGDV
160 170 180 190
EIQKGLGVKG KSQFDSNITA PDAIINGKST DKHIHRGDSG GTTGPMQ
Length:197
Mass (Da):21,689
Last modified:May 1, 2000 - v1
Checksum:i448C7A7ADCADD5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01123.1.
RefSeqiNP_050649.1. NC_000929.1.

Genome annotation databases

GeneIDi2636280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01123.1 .
RefSeqi NP_050649.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VTN X-ray 1.75 A 100-197 [» ]
3VTO X-ray 1.44 A/B/C/P/Q/R 92-197 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2636280.

Family and domain databases

InterProi IPR013046. Phage_Mu/P2_baseplate_assem.
IPR014462. Phage_Mu_Gp45.
[Graphical view ]
Pfami PF06890. Phage_Mu_Gp45. 1 hit.
[Graphical view ]
PIRSFi PIRSF012337. gp45. 1 hit.
TIGRFAMsi TIGR01644. phage_P2_V. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
    Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
    J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu."
    Grundy F.J., Howe M.M.
    Virology 143:485-504(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  3. "Mutational analysis of a C-dependent late promoter of bacteriophage Mu."
    Chiang L.W., Howe M.M.
    Genetics 135:619-629(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "The C-terminal domain is sufficient for host-binding activity of the Mu phage tail-spike protein."
    Suzuki H., Yamada S., Toyama Y., Takeda S.
    Biochim. Biophys. Acta 1804:1738-1742(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "Contractile tail machines of bacteriophages."
    Leiman P.G., Shneider M.M.
    Adv. Exp. Med. Biol. 726:93-114(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
    Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
    Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.

Entry informationi

Entry nameiBP45_BPMU
AccessioniPrimary (citable) accession number: Q9T1V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Translation initiates from a non-canonical start codon (GUG).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3