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Q9T0N8

- CKX1_MAIZE

UniProt

Q9T0N8 - CKX1_MAIZE

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Protein
Cytokinin dehydrogenase 1
Gene
CKX1
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.

Catalytic activityi

N(6)-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor.

Cofactori

FAD.

Enzyme regulationi

Competitive inhibition by phenylureas.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101FAD; via carbonyl oxygen By similarity
Binding sitei169 – 1691FAD; via amide nitrogen By similarity
Binding sitei174 – 1741FAD By similarity
Binding sitei235 – 2351FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei491 – 4911FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1045FAD By similarity
Nucleotide bindingi105 – 1062FAD By similarity
Nucleotide bindingi180 – 1845FAD By similarity
Nucleotide bindingi527 – 5304FAD By similarity

GO - Molecular functioni

  1. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  2. cytokinin dehydrogenase activity Source: UniProtKB-EC
  3. flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  1. cytokinin metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:CKX1-MONOMER.
BRENDAi1.5.99.12. 6752.
SABIO-RKQ9T0N8.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinin dehydrogenase 1 (EC:1.5.99.12)
Alternative name(s):
Cytokinin oxidase 1
Short name:
CKO 1
Short name:
COX 1
ZmCKX1
Gene namesi
Name:CKX1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ9T0N8.
MaizeGDBi194080.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 534516Cytokinin dehydrogenase 1
PRO_0000020424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi52 – 521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Modified residuei105 – 1051Pros-8alpha-FAD histidine
Glycosylationi134 – 1341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi294 – 2941N-linked (GlcNAc...) Reviewed prediction
Glycosylationi323 – 3231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi338 – 3381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi434 – 4341N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated; with approximately 10 hexose residues per site.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in immature kernels and unpollinated cobs. Weakly expressed in kernels harvested two weeks after anthesis.1 Publication

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni41 – 455
Helixi51 – 566
Beta strandi70 – 734
Helixi78 – 9013
Beta strandi98 – 1036
Beta strandi107 – 1093
Beta strandi116 – 1205
Helixi121 – 1255
Beta strandi127 – 1304
Beta strandi132 – 1354
Beta strandi139 – 1457
Helixi150 – 1589
Turni159 – 1613
Beta strandi162 – 1654
Beta strandi169 – 1713
Helixi175 – 1795
Helixi188 – 1914
Helixi194 – 1963
Beta strandi197 – 2059
Beta strandi210 – 2189
Helixi219 – 2257
Beta strandi232 – 24413
Beta strandi247 – 25711
Helixi259 – 27012
Beta strandi285 – 2939
Helixi294 – 2963
Helixi297 – 3026
Beta strandi305 – 3073
Helixi309 – 32113
Beta strandi325 – 33612
Helixi345 – 35410
Beta strandi364 – 3707
Helixi371 – 3755
Helixi377 – 38711
Beta strandi391 – 3933
Beta strandi398 – 4036
Helixi404 – 4063
Helixi407 – 4148
Turni415 – 4217
Beta strandi428 – 4347
Helixi435 – 4373
Beta strandi448 – 45710
Helixi466 – 48318
Beta strandi489 – 4924
Helixi498 – 5058
Helixi507 – 52014
Helixi528 – 5303

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W1OX-ray1.70A1-534[»]
1W1QX-ray1.80A1-534[»]
1W1RX-ray1.90A1-534[»]
1W1SX-ray2.00A1-534[»]
2QKNX-ray2.15A19-534[»]
2QPMX-ray1.85A19-534[»]
3BW7X-ray1.95A19-534[»]
3C0PX-ray1.95A19-534[»]
3DQ0X-ray1.90A19-534[»]
3KJMX-ray1.90A19-534[»]
3S1CX-ray2.09A19-534[»]
3S1DX-ray1.75A19-534[»]
3S1EX-ray1.90A19-534[»]
3S1FX-ray2.00A19-534[»]
ProteinModelPortaliQ9T0N8.
SMRiQ9T0N8. Positions 32-534.

Miscellaneous databases

EvolutionaryTraceiQ9T0N8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 245181FAD-binding PCMH-type
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi339 – 3457Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK00279.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR015345. Cytokinin_DH_FAD/cytokin-bd.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
[Graphical view]
PfamiPF09265. Cytokin-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9T0N8-1 [UniParc]FASTAAdd to Basket

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MAVVYYLLLA GLIACSHALA AGTPALGDDR GRPWPASLAA LALDGKLRTD    50
SNATAAASTD FGNITSALPA AVLYPSSTGD LVALLSAANS TPGWPYTIAF 100
RGRGHSLMGQ AFAPGGVVVN MASLGDAAAP PRINVSADGR YVDAGGEQVW 150
IDVLRASLAR GVAPRSWNDY LYLTVGGTLS NAGISGQAFR HGPQISNVLE 200
MDVITGHGEM VTCSKQLNAD LFDAVLGGLG QFGVITRARI AVEPAPARAR 250
WVRFVYTDFA AFSADQERLT APRPGGGGAS FGPMSYVEGS VFVNQSLATD 300
LANTGFFTDA DVARIVALAG ERNATTVYSI EATLNYDNAT AAAAAVDQEL 350
ASVLGTLSYV EGFAFQRDVA YAAFLDRVHG EEVALNKLGL WRVPHPWLNM 400
FVPRSRIADF DRGVFKGILQ GTDIVGPLIV YPLNKSMWDD GMSAATPSED 450
VFYAVSLLFS SVAPNDLARL QEQNRRILRF CDLAGIQYKT YLARHTDRSD 500
WVRHFGAAKW NRFVEMKNKY DPKRLLSPGQ DIFN 534
Length:534
Mass (Da):57,229
Last modified:May 24, 2005 - v2
Checksum:i13FB5AF654C169E5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791G → A in CAA77151. 1 Publication
Sequence conflicti168 – 1681N → T in CAA77151. 1 Publication
Sequence conflicti254 – 2541F → L in CAA77151. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18377 mRNA. Translation: CAA77151.1.
AF044603 Genomic DNA. Translation: AAC27500.1.
PIRiT01500.
T51929.
RefSeqiNP_001105591.1. NM_001112121.1.
UniGeneiZm.209.

Genome annotation databases

GeneIDi542585.
KEGGizma:542585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18377 mRNA. Translation: CAA77151.1 .
AF044603 Genomic DNA. Translation: AAC27500.1 .
PIRi T01500.
T51929.
RefSeqi NP_001105591.1. NM_001112121.1.
UniGenei Zm.209.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W1O X-ray 1.70 A 1-534 [» ]
1W1Q X-ray 1.80 A 1-534 [» ]
1W1R X-ray 1.90 A 1-534 [» ]
1W1S X-ray 2.00 A 1-534 [» ]
2QKN X-ray 2.15 A 19-534 [» ]
2QPM X-ray 1.85 A 19-534 [» ]
3BW7 X-ray 1.95 A 19-534 [» ]
3C0P X-ray 1.95 A 19-534 [» ]
3DQ0 X-ray 1.90 A 19-534 [» ]
3KJM X-ray 1.90 A 19-534 [» ]
3S1C X-ray 2.09 A 19-534 [» ]
3S1D X-ray 1.75 A 19-534 [» ]
3S1E X-ray 1.90 A 19-534 [» ]
3S1F X-ray 2.00 A 19-534 [» ]
ProteinModelPortali Q9T0N8.
SMRi Q9T0N8. Positions 32-534.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL5363.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542585.
KEGGi zma:542585.

Organism-specific databases

Gramenei Q9T0N8.
MaizeGDBi 194080.

Phylogenomic databases

KOi K00279.

Enzyme and pathway databases

BioCyci MetaCyc:CKX1-MONOMER.
BRENDAi 1.5.99.12. 6752.
SABIO-RK Q9T0N8.

Miscellaneous databases

EvolutionaryTracei Q9T0N8.

Family and domain databases

Gene3Di 3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProi IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR015345. Cytokinin_DH_FAD/cytokin-bd.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
[Graphical view ]
Pfami PF09265. Cytokin-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view ]
SUPFAMi SSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEi PS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts."
    Houba-Herin N., Pethe C., D'Alayer J., Laloue M.
    Plant J. 17:615-626(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 417-435; 490-517 AND 524-534.
    Strain: cv. Nobilis.
    Tissue: Kernel.
  2. "Isolation of a gene encoding a glycosylated cytokinin oxidase from maize."
    Morris R.O., Bilyeu K.D., Laskey J.G., Cheikh N.N.
    Biochem. Biophys. Res. Commun. 255:328-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 286-308; 369-377; 388-392 AND 417-431, IDENTIFICATION BY MASS SPECTROMETRY.
  3. "Molecular and biochemical characterization of a cytokinin oxidase from maize."
    Bilyeu K.D., Cole J.L., Laskey J.G., Riekhof W.R., Esparza T.J., Kramer M.D., Morris R.O.
    Plant Physiol. 125:378-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, TISSUE SPECIFICITY.
  4. "Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species."
    Schmuelling T., Werner T., Riefler M., Krupkova E., Bartrina y Manns I.
    J. Plant Res. 116:241-252(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.
  5. "Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis."
    Malito E., Coda A., Bilyeu K.D., Fraaije M.W., Mattevi A.
    J. Mol. Biol. 341:1237-1249(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 19-534 OF APOENZYME AND IN COMPLEX WITH BENZYLAMINOPURINE; ISOPENTENYLADENINE OR TRANS-ZEATIN.

Entry informationi

Entry nameiCKX1_MAIZE
AccessioniPrimary (citable) accession number: Q9T0N8
Secondary accession number(s): O81158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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