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Q9T0J5

- ASE3_ARATH

UniProt

Q9T0J5 - ASE3_ARATH

Protein

Amidophosphoribosyltransferase 3, chloroplastic

Gene

ASE3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine.1 Publication

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.1 Publication

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity
    Binds 1 magnesium ion per subunit.By similarity

    Enzyme regulationi

    Inhibited by the phenyltriazole acetic acid compound [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid (DAS734), a bleaching herbicide. Repressed by AMP, ADP, ATP and GTP, and slightly by GMP.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771NucleophilePROSITE-ProRule annotation
    Metal bindingi313 – 3131Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi459 – 4591Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi511 – 5111Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi514 – 5141Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    2. iron-sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G38880-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.A02.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase 3, chloroplastic (EC:2.4.2.14)
    Short name:
    AtATase3
    Short name:
    PRPP3
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase 3
    Short name:
    AtGPRAT3
    Gene namesi
    Name:ASE3
    Synonyms:GPRAT3
    Ordered Locus Names:At4g38880
    ORF Names:F19H22.3, T9A14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G38880.

    Subcellular locationi

    Plastidchloroplast stroma By similarity

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5959ChloroplastSequence AnalysisAdd
    BLAST
    Chaini60 – 532473Amidophosphoribosyltransferase 3, chloroplasticPRO_0000420283Add
    BLAST

    Proteomic databases

    PaxDbiQ9T0J5.
    PRIDEiQ9T0J5.

    Expressioni

    Tissue specificityi

    Mostly expressed at low levels in leaves, and, to a lower extent, in cotyledons.1 Publication

    Gene expression databases

    GenevestigatoriQ9T0J5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G38880.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9T0J5.
    SMRiQ9T0J5. Positions 77-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 296220Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    InParanoidiQ9T0J5.
    KOiK00764.
    OMAiPFIASCK.
    PhylomeDBiQ9T0J5.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9T0J5-1 [UniParc]FASTAAdd to Basket

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    MAFSVEEISS ILPNSLSANP RNVSQNTISP SFFKPSLKPY ASKTLISLSC    50
    RRSLSPVFSA GTYVTNVDED DKLHEECGVV GIHGDPEASR LSYLALHALQ 100
    HRGQEGAGIV AANQNGLESI TGVGLVSDVF TESKLNNLPG DIAIGHVRYS 150
    TSGASMLKNV QPFIASCKLG SLAVAHNGNF VNYKQLKTKL EEMGSIFITS 200
    SDTELVLHLI AKSKAKTFLL RVIDACEKLR GAYSMVFVFE DKLIAVRDPF 250
    GFRPLVMGRR SNGAVVFASE TCALDLIDAT YEREVCPGEI VVVDRNHGDS 300
    SMFMISHPEQ KQCVFEHGYF SQPNSIVFGR SVYETRRMYG EILATVAPVD 350
    CDVVIAVPDS GTVAALGYAA KAGVPFQIGL LRSHYAKRTF IEPTQEIRDF 400
    AVKVKLSPVR AVLEGKRVVV VDDSIVRGTT SLKIVRMLRD AGAKEVHMRI 450
    ALPPMIASCY YGVDTPRSQE LISSKMSVEA IQKHINCDSL AFLPLDSLKG 500
    VYGPVESHRY CYACFTGKYP VTKTESEEAD AS 532
    Length:532
    Mass (Da):58,041
    Last modified:May 1, 2000 - v1
    Checksum:iC1B67B430D36A775
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035656 Genomic DNA. Translation: CAB38622.1.
    AL161594 Genomic DNA. Translation: CAB80551.1.
    CP002687 Genomic DNA. Translation: AEE86986.1.
    PIRiT06087.
    RefSeqiNP_195599.1. NM_120048.1.
    UniGeneiAt.54650.

    Genome annotation databases

    EnsemblPlantsiAT4G38880.1; AT4G38880.1; AT4G38880.
    GeneIDi830043.
    KEGGiath:AT4G38880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035656 Genomic DNA. Translation: CAB38622.1 .
    AL161594 Genomic DNA. Translation: CAB80551.1 .
    CP002687 Genomic DNA. Translation: AEE86986.1 .
    PIRi T06087.
    RefSeqi NP_195599.1. NM_120048.1.
    UniGenei At.54650.

    3D structure databases

    ProteinModelPortali Q9T0J5.
    SMRi Q9T0J5. Positions 77-520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G38880.1-P.

    Protein family/group databases

    MEROPSi C44.A02.

    Proteomic databases

    PaxDbi Q9T0J5.
    PRIDEi Q9T0J5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G38880.1 ; AT4G38880.1 ; AT4G38880 .
    GeneIDi 830043.
    KEGGi ath:AT4G38880.

    Organism-specific databases

    GeneFarmi 2376. 186.
    TAIRi AT4G38880.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    InParanoidi Q9T0J5.
    KOi K00764.
    OMAi PFIASCK.
    PhylomeDBi Q9T0J5.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci ARA:AT4G38880-MONOMER.

    Gene expression databases

    Genevestigatori Q9T0J5.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants."
      Hung W.-F., Chen L.-J., Boldt R., Sun C.-W., Li H.-M.
      Plant Physiol. 135:1314-1323(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    4. "Chemical genetic identification of glutamine phosphoribosylpyrophosphate amidotransferase as the target for a novel bleaching herbicide in Arabidopsis."
      Walsh T.A., Bauer T., Neal R., Merlo A.O., Schmitzer P.R., Hicks G.R., Honma M., Matsumura W., Wolff K., Davies J.P.
      Plant Physiol. 144:1292-1304(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiASE3_ARATH
    AccessioniPrimary (citable) accession number: Q9T0J5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3