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Protein

Amidophosphoribosyltransferase 3, chloroplastic

Gene

ASE3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine.1 Publication

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by the phenyltriazole acetic acid compound [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid (DAS734), a bleaching herbicide. Repressed by AMP, ADP, ATP and GTP, and slightly by GMP.1 Publication

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase 2, chloroplastic (ASE2), Amidophosphoribosyltransferase (AXX17_At4g39730), Amidophosphoribosyltransferase 1, chloroplastic (ASE1), Amidophosphoribosyltransferase 3, chloroplastic (ASE3), Amidophosphoribosyltransferase (AXX17_At4g44210), Amidophosphoribosyltransferase (AXX17_At2g11630)
  2. Phosphoribosylamine--glycine ligase, chloroplastic (PUR2)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei77NucleophilePROSITE-ProRule annotation1
Metal bindingi313Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi459Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi511Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi514Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandIron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G38880-MONOMER
BRENDAi2.4.2.14 399
ReactomeiR-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.A02

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase 3, chloroplastic (EC:2.4.2.14)
Short name:
AtATase3
Short name:
PRPP3
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase 3
Short name:
AtGPRAT3
Gene namesi
Name:ASE3
Synonyms:GPRAT3
Ordered Locus Names:At4g38880
ORF Names:F19H22.3, T9A14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G38880
TAIRilocus:2141811 AT4G38880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 59ChloroplastSequence analysisAdd BLAST59
ChainiPRO_000042028360 – 532Amidophosphoribosyltransferase 3, chloroplasticAdd BLAST473

Proteomic databases

PaxDbiQ9T0J5

Expressioni

Tissue specificityi

Mostly expressed at low levels in leaves, and, to a lower extent, in cotyledons.1 Publication

Gene expression databases

ExpressionAtlasiQ9T0J5 baseline and differential
GenevisibleiQ9T0J5 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G38880.1

Structurei

3D structure databases

ProteinModelPortaliQ9T0J5
SMRiQ9T0J5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 296Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST220

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

eggNOGiKOG0572 Eukaryota
COG0034 LUCA
HOGENOMiHOG000033688
InParanoidiQ9T0J5
KOiK00764
OMAiPFIASCK
OrthoDBiEOG093609B8
PhylomeDBiQ9T0J5

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.103 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9T0J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSVEEISS ILPNSLSANP RNVSQNTISP SFFKPSLKPY ASKTLISLSC
60 70 80 90 100
RRSLSPVFSA GTYVTNVDED DKLHEECGVV GIHGDPEASR LSYLALHALQ
110 120 130 140 150
HRGQEGAGIV AANQNGLESI TGVGLVSDVF TESKLNNLPG DIAIGHVRYS
160 170 180 190 200
TSGASMLKNV QPFIASCKLG SLAVAHNGNF VNYKQLKTKL EEMGSIFITS
210 220 230 240 250
SDTELVLHLI AKSKAKTFLL RVIDACEKLR GAYSMVFVFE DKLIAVRDPF
260 270 280 290 300
GFRPLVMGRR SNGAVVFASE TCALDLIDAT YEREVCPGEI VVVDRNHGDS
310 320 330 340 350
SMFMISHPEQ KQCVFEHGYF SQPNSIVFGR SVYETRRMYG EILATVAPVD
360 370 380 390 400
CDVVIAVPDS GTVAALGYAA KAGVPFQIGL LRSHYAKRTF IEPTQEIRDF
410 420 430 440 450
AVKVKLSPVR AVLEGKRVVV VDDSIVRGTT SLKIVRMLRD AGAKEVHMRI
460 470 480 490 500
ALPPMIASCY YGVDTPRSQE LISSKMSVEA IQKHINCDSL AFLPLDSLKG
510 520 530
VYGPVESHRY CYACFTGKYP VTKTESEEAD AS
Length:532
Mass (Da):58,041
Last modified:May 1, 2000 - v1
Checksum:iC1B67B430D36A775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035656 Genomic DNA Translation: CAB38622.1
AL161594 Genomic DNA Translation: CAB80551.1
CP002687 Genomic DNA Translation: AEE86986.1
PIRiT06087
RefSeqiNP_195599.1, NM_120048.1
UniGeneiAt.54650

Genome annotation databases

EnsemblPlantsiAT4G38880.1; AT4G38880.1; AT4G38880
GeneIDi830043
GrameneiAT4G38880.1; AT4G38880.1; AT4G38880
KEGGiath:AT4G38880

Similar proteinsi

Entry informationi

Entry nameiASE3_ARATH
AccessioniPrimary (citable) accession number: Q9T0J5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome