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Protein

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1

Gene

MTN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme of the methionine cycle that catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Contributes to the maintenance of AdoMet homeostasis and is required to sustain high rates of ethylene synthesis. Inactive towards S-adenosylhomocysteine (SAH/AdoHcy).3 Publications

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine.

Enzyme regulationi

Inhibited by CBL3 in a calcium-dependent manner.1 Publication

Kineticsi

  1. KM=7.1 µM for 5'-methylthioadenosine (MTA)1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: L-methionine biosynthesis via salvage pathway

    This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1 (MTN1), 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2 (MTN2)
    2. Methylthioribose kinase (MTK)
    This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei38Proton acceptorBy similarity1
    Binding sitei181Substrate; via carbonyl oxygen2 Publications1
    Active sitei225Proton donorCurated1

    GO - Molecular functioni

    GO - Biological processi

    • L-methionine salvage from methylthioadenosine Source: TAIR
    • nucleoside metabolic process Source: InterPro
    • phloem or xylem histogenesis Source: TAIR
    • reproduction Source: TAIR

    Keywordsi

    Molecular functionHydrolase
    Biological processAmino-acid biosynthesis, Methionine biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT4G38800-MONOMER
    BRENDAi2.4.2.28 399
    3.2.2.9 399
    UniPathwayiUPA00904; UER00871

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1 (EC:3.2.2.9)
    Short name:
    AtMTN1
    Alternative name(s):
    5'-methylthioadenosine nucleosidase
    Short name:
    MTA nucleosidase
    MTA/SAH nucleosidase 1
    Short name:
    AtMTAN1
    S-adenosylhomocysteine nucleosidase
    Short name:
    AdoHcy nucleosidase
    Short name:
    SAH nucleosidase
    Short name:
    SRH nucleosidase
    Gene namesi
    Name:MTN1
    Synonyms:MTAN, MTAN1
    Ordered Locus Names:At4g38800
    ORF Names:T9A14.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G38800
    TAIRilocus:2141806 AT4G38800

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition. Not able to grow with methylthioadenosine (MTA) as unique source of sulfur.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004013731 – 2675'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1Add BLAST267

    Proteomic databases

    PaxDbiQ9T0I8
    PRIDEiQ9T0I8

    PTM databases

    iPTMnetiQ9T0I8

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, cauline leaves and flowers.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9T0I8 baseline and differential
    GenevisibleiQ9T0I8 AT

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CBL3 in a calcium-dependent manner.4 Publications

    Protein-protein interaction databases

    BioGridi15315, 5 interactors
    IntActiQ9T0I8, 2 interactors
    STRINGi3702.AT4G38800.1

    Structurei

    Secondary structure

    1267
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi28 – 32Combined sources5
    Helixi36 – 45Combined sources10
    Beta strandi63 – 69Combined sources7
    Beta strandi72 – 78Combined sources7
    Turni83 – 85Combined sources3
    Beta strandi86 – 88Combined sources3
    Helixi92 – 106Combined sources15
    Beta strandi109 – 119Combined sources11
    Helixi121 – 123Combined sources3
    Beta strandi130 – 140Combined sources11
    Helixi148 – 153Combined sources6
    Helixi162 – 168Combined sources7
    Beta strandi171 – 177Combined sources7
    Helixi185 – 193Combined sources9
    Beta strandi197 – 202Combined sources6
    Helixi203 – 212Combined sources10
    Beta strandi217 – 226Combined sources10
    Beta strandi229 – 231Combined sources3
    Helixi233 – 259Combined sources27
    Helixi264 – 266Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2H8GX-ray1.50A/B1-267[»]
    2QSUX-ray2.00A/B1-267[»]
    2QTGX-ray1.84A/B1-267[»]
    2QTTX-ray1.93A/B1-267[»]
    3LGSX-ray2.20A/B/C/D1-267[»]
    ProteinModelPortaliQ9T0I8
    SMRiQ9T0I8
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9T0I8

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni116 – 118Substrate binding3
    Regioni199 – 202Substrate binding4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410IEX0 Eukaryota
    ENOG4111IXC LUCA
    HOGENOMiHOG000241650
    KOiK01244
    OMAiRFYGNYK
    OrthoDBiEOG09360KSB
    PhylomeDBiQ9T0I8

    Family and domain databases

    InterProiView protein in InterPro
    IPR000845 Nucleoside_phosphorylase_d
    IPR035994 Nucleoside_phosphorylase_sf
    PfamiView protein in Pfam
    PF01048 PNP_UDP_1, 1 hit
    SUPFAMiSSF53167 SSF53167, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9T0I8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAPHGDGLSD IEEPEVDAQS EILRPISSVV FVIAMQAEAL PLVNKFGLSE
    60 70 80 90 100
    TTDSPLGKGL PWVLYHGVHK DLRINVVCPG RDAALGIDSV GTVPASLITF
    110 120 130 140 150
    ASIQALKPDI IINAGTCGGF KVKGANIGDV FLVSDVVFHD RRIPIPMFDL
    160 170 180 190 200
    YGVGLRQAFS TPNLLKELNL KIGRLSTGDS LDMSTQDETL IIANDATLKD
    210 220 230 240 250
    MEGAAVAYVA DLLKIPVVFL KAVTDLVDGD KPTAEEFLQN LTVVTAALEG
    260
    TATKVINFIN GRNLSDL
    Length:267
    Mass (Da):28,451
    Last modified:May 1, 2000 - v1
    Checksum:iE7F8A113441AA012
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035656 Genomic DNA Translation: CAB38614.1
    AL161594 Genomic DNA Translation: CAB80543.1
    CP002687 Genomic DNA Translation: AEE86976.1
    AF370297 mRNA Translation: AAK44112.1
    AY142681 mRNA Translation: AAN13219.1
    PIRiT06079
    RefSeqiNP_195591.1, NM_120040.4
    UniGeneiAt.2827
    At.31148

    Genome annotation databases

    EnsemblPlantsiAT4G38800.1; AT4G38800.1; AT4G38800
    GeneIDi830035
    GrameneiAT4G38800.1; AT4G38800.1; AT4G38800
    KEGGiath:AT4G38800

    Similar proteinsi

    Entry informationi

    Entry nameiMTN1_ARATH
    AccessioniPrimary (citable) accession number: Q9T0I8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 1, 2000
    Last modified: April 25, 2018
    This is version 101 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
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    Main funding by: National Institutes of Health