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Protein

Long chain acyl-CoA synthetase 4

Gene

LACS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi228 – 23912ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G23850-MONOMER.
MetaCyc:AT4G23850-MONOMER.
ReactomeiREACT_235205. Synthesis of very long-chain fatty acyl-CoAs.
SABIO-RKQ9T0A0.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 4 (EC:6.2.1.3)
Gene namesi
Name:LACS4
Ordered Locus Names:At4g23850
ORF Names:T32A16_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G23850.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Long chain acyl-CoA synthetase 4PRO_0000401412Add
BLAST

Proteomic databases

PaxDbiQ9T0A0.
PRIDEiQ9T0A0.

Expressioni

Gene expression databases

GenevestigatoriQ9T0A0.

Interactioni

Protein-protein interaction databases

BioGridi13773. 1 interaction.
STRINGi3702.AT4G23850.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9T0A0.
SMRiQ9T0A0. Positions 51-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni495 – 51925Fatty acid-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ9T0A0.
KOiK01897.
OMAiVGKGGRC.
PhylomeDBiQ9T0A0.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9T0A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQKKYIFQ VEEGKEGSDG RPSVGPVYRS IFAKDGFPDP IEGMDSCWDV
60 70 80 90 100
FRMSVEKYPN NPMLGRREIV DGKPGKYVWQ TYQEVYDIVM KLGNSLRSVG
110 120 130 140 150
VKDEAKCGIY GANSPEWIIS MEACNAHGLY CVPLYDTLGA DAVEFIISHS
160 170 180 190 200
EVSIVFVEEK KISELFKTCP NSTEYMKTVV SFGGVSREQK EEAETFGLVI
210 220 230 240 250
YAWDEFLKLG EGKQYDLPIK KKSDICTIMY TSGTTGDPKG VMISNESIVT
260 270 280 290 300
LIAGVIRLLK SANEALTVKD VYLSYLPLAH IFDRVIEECF IQHGAAIGFW
310 320 330 340 350
RGDVKLLIED LAELKPTIFC AVPRVLDRVY SGLQKKLSDG GFLKKFIFDS
360 370 380 390 400
AFSYKFGYMK KGQSHVEASP LFDKLVFSKV KQGLGGNVRI ILSGAAPLAS
410 420 430 440 450
HVESFLRVVA CCHVLQGYGL TESCAGTFVS LPDELGMLGT VGPPVPNVDI
460 470 480 490 500
RLESVPEMEY DALASTARGE ICIRGKTLFS GYYKREDLTK EVLIDGWLHT
510 520 530 540 550
GDVGEWQPDG SMKIIDRKKN IFKLSQGEYV AVENIENIYG EVQAVDSVWV
560 570 580 590 600
YGNSFESFLI AIANPNQHIL ERWAAENGVS GDYDALCQNE KAKEFILGEL
610 620 630 640 650
VKMAKEKKMK GFEIIKAIHL DPVPFDMERD LLTPTFKKKR PQLLKYYQSV
660
IDEMYKTINA KFASRG
Length:666
Mass (Da):74,508
Last modified:May 1, 2000 - v1
Checksum:i8E663756D60B0E57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503754 mRNA. Translation: AAM28871.1.
AL078468 Genomic DNA. Translation: CAB43885.1.
AL161560 Genomic DNA. Translation: CAB81303.1.
CP002687 Genomic DNA. Translation: AEE84812.1.
AY049239 mRNA. Translation: AAK83581.1.
PIRiT08904.
RefSeqiNP_194116.1. NM_118516.4.
UniGeneiAt.3384.

Genome annotation databases

EnsemblPlantsiAT4G23850.1; AT4G23850.1; AT4G23850.
GeneIDi828484.
KEGGiath:AT4G23850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503754 mRNA. Translation: AAM28871.1.
AL078468 Genomic DNA. Translation: CAB43885.1.
AL161560 Genomic DNA. Translation: CAB81303.1.
CP002687 Genomic DNA. Translation: AEE84812.1.
AY049239 mRNA. Translation: AAK83581.1.
PIRiT08904.
RefSeqiNP_194116.1. NM_118516.4.
UniGeneiAt.3384.

3D structure databases

ProteinModelPortaliQ9T0A0.
SMRiQ9T0A0. Positions 51-563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13773. 1 interaction.
STRINGi3702.AT4G23850.1-P.

Proteomic databases

PaxDbiQ9T0A0.
PRIDEiQ9T0A0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G23850.1; AT4G23850.1; AT4G23850.
GeneIDi828484.
KEGGiath:AT4G23850.

Organism-specific databases

TAIRiAT4G23850.

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ9T0A0.
KOiK01897.
OMAiVGKGGRC.
PhylomeDBiQ9T0A0.

Enzyme and pathway databases

UniPathwayiUPA00199.
BioCyciARA:AT4G23850-MONOMER.
MetaCyc:AT4G23850-MONOMER.
ReactomeiREACT_235205. Synthesis of very long-chain fatty acyl-CoAs.
SABIO-RKQ9T0A0.

Gene expression databases

GenevestigatoriQ9T0A0.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.

Entry informationi

Entry nameiLACS4_ARATH
AccessioniPrimary (citable) accession number: Q9T0A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.