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Q9T0A0

- LACS4_ARATH

UniProt

Q9T0A0 - LACS4_ARATH

Protein

Long chain acyl-CoA synthetase 4

Gene

LACS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi228 – 23912ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G23850-MONOMER.
    MetaCyc:AT4G23850-MONOMER.
    SABIO-RKQ9T0A0.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 4 (EC:6.2.1.3)
    Gene namesi
    Name:LACS4
    Ordered Locus Names:At4g23850
    ORF Names:T32A16_20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G23850.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: TAIR
    2. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 666666Long chain acyl-CoA synthetase 4PRO_0000401412Add
    BLAST

    Proteomic databases

    PaxDbiQ9T0A0.
    PRIDEiQ9T0A0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9T0A0.

    Interactioni

    Protein-protein interaction databases

    BioGridi13773. 1 interaction.
    STRINGi3702.AT4G23850.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9T0A0.
    SMRiQ9T0A0. Positions 51-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni495 – 51925Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ9T0A0.
    KOiK01897.
    OMAiICIRGDT.
    PhylomeDBiQ9T0A0.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9T0A0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQQKKYIFQ VEEGKEGSDG RPSVGPVYRS IFAKDGFPDP IEGMDSCWDV    50
    FRMSVEKYPN NPMLGRREIV DGKPGKYVWQ TYQEVYDIVM KLGNSLRSVG 100
    VKDEAKCGIY GANSPEWIIS MEACNAHGLY CVPLYDTLGA DAVEFIISHS 150
    EVSIVFVEEK KISELFKTCP NSTEYMKTVV SFGGVSREQK EEAETFGLVI 200
    YAWDEFLKLG EGKQYDLPIK KKSDICTIMY TSGTTGDPKG VMISNESIVT 250
    LIAGVIRLLK SANEALTVKD VYLSYLPLAH IFDRVIEECF IQHGAAIGFW 300
    RGDVKLLIED LAELKPTIFC AVPRVLDRVY SGLQKKLSDG GFLKKFIFDS 350
    AFSYKFGYMK KGQSHVEASP LFDKLVFSKV KQGLGGNVRI ILSGAAPLAS 400
    HVESFLRVVA CCHVLQGYGL TESCAGTFVS LPDELGMLGT VGPPVPNVDI 450
    RLESVPEMEY DALASTARGE ICIRGKTLFS GYYKREDLTK EVLIDGWLHT 500
    GDVGEWQPDG SMKIIDRKKN IFKLSQGEYV AVENIENIYG EVQAVDSVWV 550
    YGNSFESFLI AIANPNQHIL ERWAAENGVS GDYDALCQNE KAKEFILGEL 600
    VKMAKEKKMK GFEIIKAIHL DPVPFDMERD LLTPTFKKKR PQLLKYYQSV 650
    IDEMYKTINA KFASRG 666
    Length:666
    Mass (Da):74,508
    Last modified:May 1, 2000 - v1
    Checksum:i8E663756D60B0E57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503754 mRNA. Translation: AAM28871.1.
    AL078468 Genomic DNA. Translation: CAB43885.1.
    AL161560 Genomic DNA. Translation: CAB81303.1.
    CP002687 Genomic DNA. Translation: AEE84812.1.
    AY049239 mRNA. Translation: AAK83581.1.
    PIRiT08904.
    RefSeqiNP_194116.1. NM_118516.4.
    UniGeneiAt.3384.

    Genome annotation databases

    EnsemblPlantsiAT4G23850.1; AT4G23850.1; AT4G23850.
    GeneIDi828484.
    KEGGiath:AT4G23850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503754 mRNA. Translation: AAM28871.1 .
    AL078468 Genomic DNA. Translation: CAB43885.1 .
    AL161560 Genomic DNA. Translation: CAB81303.1 .
    CP002687 Genomic DNA. Translation: AEE84812.1 .
    AY049239 mRNA. Translation: AAK83581.1 .
    PIRi T08904.
    RefSeqi NP_194116.1. NM_118516.4.
    UniGenei At.3384.

    3D structure databases

    ProteinModelPortali Q9T0A0.
    SMRi Q9T0A0. Positions 51-563.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13773. 1 interaction.
    STRINGi 3702.AT4G23850.1-P.

    Proteomic databases

    PaxDbi Q9T0A0.
    PRIDEi Q9T0A0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G23850.1 ; AT4G23850.1 ; AT4G23850 .
    GeneIDi 828484.
    KEGGi ath:AT4G23850.

    Organism-specific databases

    TAIRi AT4G23850.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q9T0A0.
    KOi K01897.
    OMAi ICIRGDT.
    PhylomeDBi Q9T0A0.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT4G23850-MONOMER.
    MetaCyc:AT4G23850-MONOMER.
    SABIO-RK Q9T0A0.

    Gene expression databases

    Genevestigatori Q9T0A0.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.

    Entry informationi

    Entry nameiLACS4_ARATH
    AccessioniPrimary (citable) accession number: Q9T0A0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3