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Protein

1-aminocyclopropane-1-carboxylate synthase 8

Gene

ACS8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene.

Miscellaneous

The stability of ACS proteins, and the regulation of such stability, play a central role in ethylene biosynthesis.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.1 Publication

Cofactori

Kineticsi

  1. KM=15 µM for AdoMet
  1. Vmax=143 µM/h/mg enzyme

pH dependencei

Optimum pH is 8.2.

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47SubstrateBy similarity1
Binding sitei85SubstrateBy similarity1

GO - Molecular functioni

  • 1-aminocyclopropane-1-carboxylate synthase activity Source: TAIR
  • identical protein binding Source: IntAct
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • ethylene biosynthetic process Source: TAIR
  • fruit ripening Source: UniProtKB-KW

Keywordsi

Molecular functionLyase
Biological processEthylene biosynthesis, Fruit ripening
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ9T065
UniPathwayiUPA00384; UER00562

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 8 (EC:4.4.1.14)
Short name:
ACC synthase 8
Alternative name(s):
S-adenosyl-L-methionine methylthioadenosine-lyase 8
Gene namesi
Name:ACS8
Ordered Locus Names:At4g37770
ORF Names:T28I19.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37770
TAIRilocus:2137579 AT4G37770

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239021 – 4691-aminocyclopropane-1-carboxylate synthase 8Add BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

May be processed at its C-terminus.

Proteomic databases

PaxDbiQ9T065

Expressioni

Tissue specificityi

Expressed in roots. Expressed at low level in flowers and siliques.1 Publication

Inductioni

By indole-3-acetic acid (IAA) and cycloheximide (CHX).1 Publication

Gene expression databases

ExpressionAtlasiQ9T065 baseline and differential
GenevisibleiQ9T065 AT

Interactioni

Subunit structurei

Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity). Interacts with GRF3.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi15214, 2 interactors
IntActiQ9T065, 2 interactors
STRINGi3702.AT4G37770.1

Structurei

3D structure databases

ProteinModelPortaliQ9T065
SMRiQ9T065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256 Eukaryota
COG0436 LUCA
HOGENOMiHOG000011234
InParanoidiQ9T065
KOiK01762
OMAiPYYGAIT
OrthoDBiEOG093608EZ
PhylomeDBiQ9T065

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9T065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSKKASC NTHGQDSSYF WGWEEYEKNP YDEIKNPDGI IQMGLAENQL
60 70 80 90 100
SFDLIESWLA KNPDAANFQR EGQSIFRELA LFQDYHGLPS FKNAMADFMS
110 120 130 140 150
ENRGNRVSFN PNKLVLTAGA TPANETLMFC LADPGDAFLL PTPYYPGFDR
160 170 180 190 200
DLKWRTGAEI VPIQCKSANG FRITKVALEE AYEQAQKLNL KVKGVLITNP
210 220 230 240 250
SNPLGTTTTR TELNHLLDFI SRKKIHLISD EIYSGTVFTN PGFISVMEVL
260 270 280 290 300
KDRKLENTDV FDRVHIVYSL SKDLGLPGFR VGVIYSNDDF VVSAATKMSS
310 320 330 340 350
FGLISSQTQY LLSALLSDKT FTKNYLEENQ IRLKNRHKKL VSGLEAAGIE
360 370 380 390 400
CLKSNAGLFC WVDMRHLLKS NTFEAEIELW KKIVYEVKLN ISPGSSCHCN
410 420 430 440 450
EPGWFRVCFA NLSEETLKVA LDRLKRFVDG PSPTRRSQSE HQRLKNLRKM
460
KVSNWVFRLS FHDREPEER
Length:469
Mass (Da):53,371
Last modified:May 1, 2000 - v1
Checksum:i7BB3CE13E87BDDCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035709 Genomic DNA Translation: CAB38925.1
AL161592 Genomic DNA Translation: CAB80442.1
CP002687 Genomic DNA Translation: AEE86836.1
AF334712 mRNA Translation: AAG50090.1
PIRiT06024
RefSeqiNP_195491.1, NM_119939.3
UniGeneiAt.2875

Genome annotation databases

EnsemblPlantsiAT4G37770.1; AT4G37770.1; AT4G37770
GeneIDi829933
GrameneiAT4G37770.1; AT4G37770.1; AT4G37770
KEGGiath:AT4G37770

Similar proteinsi

Entry informationi

Entry namei1A18_ARATH
AccessioniPrimary (citable) accession number: Q9T065
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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