Reviewed, UniProtKB/Swiss-Prot Q9T052 (PLDG3_ARATH)

Names and origin

Protein names

Recommended name:
    Phospholipase D gamma 3
      Short name=AtPLDgamma3
      Short name=PLD gamma 3
    EC=3.1.4.4

Gene names

Name:	PLDGAMMA3
Ordered Locus Names:	At4g11840
ORF Names:	T26M18.50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	866 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence. Can use phosphatidylserine but prefers ethanolamine-containing lipids as substrates.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium. Requires micromolar level (PIP2-dependent).
Enzyme regulation	Inhibited by neomycin.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Found mainly associated with intracellular membranes but also with mitochondrial membranes, nuclei and clathrin-coated vesicles. Not found in chloroplast. Ref.2
Tissue specificity	Highly expressed in roots and flowers, moderately in stems, leaves and seedlings and low in siliques. Not detected in seeds.
Induction	Activated by wounding, heavy metal, methyl salicylate, osmotic and salt stresses.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. In PLD gamma, all the calcium-coordinating acidic amino-acids are conserved.
Miscellaneous	Presence of a putative myristoylation site MGXXXS (Gly-14).
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.
Caution	It is uncertain whether the sequence from 46 to 76 is encoded by an intron as for PLDGAMMA2. It is uncertain whether Met-1 or Met-11 is the initiator.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 866	866	Phospholipase D gamma 3		PRO_0000218814
Regions
	Domain	33 – 154	122	C2
	Domain	371 – 406	36	PLD phosphodiesterase 1
	Domain	712 – 739	28	PLD phosphodiesterase 2
Sites
	Active site	376	1	Potential
	Active site	378	1	Potential
	Active site	383	1	Potential
	Active site	717	1	Potential
	Active site	719	1	Potential
	Active site	724	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9T052-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6748F62F7CD4636E	FASTA	866	97,482
	10 20 30 40 50 60 MAYHPVYNET MSMGGGSSNE FGQWLDKQLV PFDTSSGSLR VELLHGNLDI WVKEAKHLPN 70 80 90 100 110 120 MDGFHNTLVG GMFFGLGRRN HKVDGENSSK ITSDPYVTVS ISGAVIGRTF VISNSENPVW 130 140 150 160 170 180 MQHFDVPVAH SAAKVHFVVK DSDIIGSQII GAVEIPTEQL CSGNRIEGLF PILNSRGKPC 190 200 210 220 230 240 KQGAVLSLSI QYIPMERMRL YQKGVGFGVE CVGVPGTYFP LRKGGRVTLY QDAHVDDGTL 250 260 270 280 290 300 PSVHLDGGIQ YRHGKCWEDM ADAIRRARRL IYITGWSVFH PVRLVRRNND PTQGTLGELL 310 320 330 340 350 360 KVKSQEGVRV LVLVWDDPTS RSLLGFSTKG LMNTSDEETR RFFKHSSVQV LLCPRYGGKG 370 380 390 400 410 420 HSFIKKSEVE TIYTHHQKTM IVDAEAAQNR RKIVAFVGGL DLCNGRFDTP KHPLFRTLKT 430 440 450 460 470 480 IHKDDFHNPN FVTTADDGPR EPWHDLHSKI DGPAAYDVLA NFEERWMKAS KPRGIGRLRT 490 500 510 520 530 540 SSDDSLLRLD RIPDIMGLSE ASSANDNDPE SWHVQVFRSI DSSSVKGFPK DPKEATGRNL 550 560 570 580 590 600 LCGKNILIDM SIHAAYVKAI RSAQHFIYIE NQYFLGSSFN WDSNKNLGAN NLIPMEIALK 610 620 630 640 650 660 IANKIRAREK FAAYIVIPMW PEGAPTSNPI QRILYWQHKT MQMMYQTIYK ALVEVGLDGQ 670 680 690 700 710 720 LEPQDFLNFF CLGTREVGTR EVPDGTVSVY NSPRKPPQLN AAQVQALKSR RFMIYVHSKG 730 740 750 760 770 780 MVVDDEFVLI GSANINQRSL EGTRDTEIAM GGYQPHHSWA KKGSRPRGQI FGYRMSLWAE 790 800 810 820 830 840 HLGFLEQEFE EPENMECVRR VRQLSELNWR QYAAEEVTEM PGHLLKYPVQ VDRTGKVSSL 850 860 PGYETFPDLG GKIIGSFLVV EENLTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis." Fan L., Zheng S., Cui D., Wang X. Plant Physiol. 119:1371-1378(1999) [PubMed: 10198096] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL078606 Genomic DNA. Translation: CAB44322.1. AL161532 Genomic DNA. Translation: CAB78227.1.
IPI	IPI00522246.
PIR	T09343.
RefSeq	NP_192921.1.
UniGene	At.48847
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9T052.
Proteomic databases
PRIDE	Q9T052.
Genome annotation databases
GeneID	826790.
GenomeReviews	Gene locus AT4G11840 in contig CT486007_GR.
KEGG	ath:AT4G11840.
NMPDR	fig|3702.1.peg.18855.
Organism-specific databases
TAIR	At4g11840.
Enzyme and pathway databases
BRENDA	3.1.4.4. 302.
Gene expression databases
ArrayExpress	Q9T052.
Genevestigator	Q9T052.
GermOnline	AT4G11840. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDG3_ARATH

Accession

Primary (citable) accession number: Q9T052
Secondary accession number(s): Q9XH77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)