Q9T051 (PLDG2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D gamma 2
Short name=AtPLDgamma2
Short name=PLD gamma 2
EC=3.1.4.4

Gene names

Name:	PLDGAMMA2
Ordered Locus Names:	At4g11830
ORF Names:	T26M18.40

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence. Can use phosphatidylserine but prefers ethanolamine-containing lipids as substrates.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium. Requires micromolar level (PIP2-dependent).
Enzyme regulation	Inhibited by neomycin.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Found mainly associated with intracellular membranes but also with mitochondrial membranes, nuclei and clathrin-coated vesicles. Not found in chloroplast. Ref.6
Tissue specificity	Highly expressed in roots and flowers, moderately in stems, leaves and seedlings and low in siliques. Not detected in seeds. Ref.3
Induction	Activated by wounding, heavy metal, methyl salicylate, osmotic and salt stresses.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. In PLD gamma, all the calcium-coordinating acidic amino-acids are conserved.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR cytosolic ribosome Inferred from direct assay. Source: TAIR membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9T051-1)

Also known as: PLDgamma2a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9T051-2)

Also known as: PLDDgamma2b;

The sequence of this isoform differs from the canonical sequence as follows:
     37-68: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Phospholipase D gamma 2		PRO_0000218813
Regions
	Domain	46 – 145	100	C2
	Domain	362 – 397	36	PLD phosphodiesterase 1
	Domain	702 – 729	28	PLD phosphodiesterase 2
Sites
	Active site	367	1	Potential
	Active site	369	1	Potential
	Active site	374	1	Potential
	Active site	707	1	Potential
	Active site	709	1	Potential
	Active site	714	1	Potential
Natural variations
	Alternative sequence	37 – 68	32	Missing in isoform 2.		VSP_026065

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PLDgamma2a) [UniParc]. Last modified June 12, 2007. Version 3. Checksum: A03DB7F8189D2B6C	FASTA	856	96,024
	10 20 30 40 50 60 MSMGGGSNHE FGQWLDQQLV PLATSSGSLM VELLHGNLDI WVKEAKHLPN MICYRNKLVG 70 80 90 100 110 120 GISFSELGRR IRKVDGEKSS KFTSDPYVTV SISGAVIGRT FVISNSENPV WMQHFDVPVA 130 140 150 160 170 180 HSAAEVHFVV KDNDPIGSKI IGVVGIPTKQ LCSGNRIEGL FPILNSSGKP CRKGAMLSLS 190 200 210 220 230 240 IQYTPMERMR LYQKGVGSGV ECVGVPGTYF PLRKGGRVTL YQDAHVDDGT LPSVHLDGGI 250 260 270 280 290 300 QYRHGKCWED MADAIRRARR LIYITGWSVF HPVRLVRRNN DPTEGTLGEL LKVKSQEGVR 310 320 330 340 350 360 VLVLVWDDPT SMSFPGFSTK GLMNTSDEET RRFFKHSSVQ VLLCPRYGGK GHSFIKKSEV 370 380 390 400 410 420 ETIYTHHQKT MIVDAEAAQN RRKIVAFVGG LDLCNGRFDT PKHSLFGTLK TLHKDDFHNP 430 440 450 460 470 480 NFVTTEDVGP REPWHDLHSK IDGPAAYDVL ANFEERWMAS KPRGIGKGRT SFDDSLLRIN 490 500 510 520 530 540 RIPDIMGLSE ASSANDNDPE SWHVQVFRSI DSTSVKGFPK DPEEATGRNL LCGKNILIDM 550 560 570 580 590 600 SIHAAYVKAI RSAQHFIYIE NQYFLGSSFN WDSNKDLGAN NLIPMEIALK IANKIRAREN 610 620 630 640 650 660 FAAYIVIPMW PEGAPTSKPI QRILYWQHKT MQMMYQTIYK ALLEVGLDGQ LEPQDFLNFF 670 680 690 700 710 720 CLGNREVGTR EVPDGTVNVY NCPRKPPQPN AAQVQALKSR RFMIYVHSKG MVVDDEFVLI 730 740 750 760 770 780 GSANINQRSL EGTRDTEIAM GGYQPHHSWA KKGSRPRGQI FGYRMSLWAE HLGFLEQEFE 790 800 810 820 830 840 EPENMECVRR VRQLSELNWG QYAAEEVTEM SGHLLKYPVQ VDKTGKVSSL PGCETFPDLG 850 GKIIGSFLTL QENLTI
	Isoform 2 (PLDDgamma2b) [UniParc]. Checksum: B3AF7072597845DE	FASTA	824	92,395
	10 20 30 40 50 60 MSMGGGSNHE FGQWLDQQLV PLATSSGSLM VELLHGRRIR KVDGEKSSKF TSDPYVTVSI 70 80 90 100 110 120 SGAVIGRTFV ISNSENPVWM QHFDVPVAHS AAEVHFVVKD NDPIGSKIIG VVGIPTKQLC 130 140 150 160 170 180 SGNRIEGLFP ILNSSGKPCR KGAMLSLSIQ YTPMERMRLY QKGVGSGVEC VGVPGTYFPL 190 200 210 220 230 240 RKGGRVTLYQ DAHVDDGTLP SVHLDGGIQY RHGKCWEDMA DAIRRARRLI YITGWSVFHP 250 260 270 280 290 300 VRLVRRNNDP TEGTLGELLK VKSQEGVRVL VLVWDDPTSM SFPGFSTKGL MNTSDEETRR 310 320 330 340 350 360 FFKHSSVQVL LCPRYGGKGH SFIKKSEVET IYTHHQKTMI VDAEAAQNRR KIVAFVGGLD 370 380 390 400 410 420 LCNGRFDTPK HSLFGTLKTL HKDDFHNPNF VTTEDVGPRE PWHDLHSKID GPAAYDVLAN 430 440 450 460 470 480 FEERWMASKP RGIGKGRTSF DDSLLRINRI PDIMGLSEAS SANDNDPESW HVQVFRSIDS 490 500 510 520 530 540 TSVKGFPKDP EEATGRNLLC GKNILIDMSI HAAYVKAIRS AQHFIYIENQ YFLGSSFNWD 550 560 570 580 590 600 SNKDLGANNL IPMEIALKIA NKIRARENFA AYIVIPMWPE GAPTSKPIQR ILYWQHKTMQ 610 620 630 640 650 660 MMYQTIYKAL LEVGLDGQLE PQDFLNFFCL GNREVGTREV PDGTVNVYNC PRKPPQPNAA 670 680 690 700 710 720 QVQALKSRRF MIYVHSKGMV VDDEFVLIGS ANINQRSLEG TRDTEIAMGG YQPHHSWAKK 730 740 750 760 770 780 GSRPRGQIFG YRMSLWAEHL GFLEQEFEEP ENMECVRRVR QLSELNWGQY AAEEVTEMSG 790 800 810 820 HLLKYPVQVD KTGKVSSLPG CETFPDLGGK IIGSFLTLQE NLTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and nucleotide sequence of the fourth phospholipase D, PLD-gamma 2, from Arabidopsis thaliana." Qin W., Dyer J.H., Zheng L., Wang X. Plant Gene Register PGR99-084 Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: cv. Columbia.
[2]	Qin W., Dyer J.H., Zheng L., Wang X. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: cv. Columbia.
[3]	"Expression and characterization of Arabidopsis phospholipase Dgamma2." Qin C., Li M., Qin W., Bahn S.C., Wang C., Wang X. Biochim. Biophys. Acta 1761:1450-1458(2006) [PubMed: 17098468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION.
[4]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis." Fan L., Zheng S., Cui D., Wang X. Plant Physiol. 119:1371-1378(1999) [PubMed: 10198096] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF138281 mRNA. Translation: AAD38519.2. DQ812124 mRNA. Translation: ABG88077.1. AL078606 Genomic DNA. Translation: CAB44321.1. AL161532 Genomic DNA. Translation: CAB78226.1. CP002687 Genomic DNA. Translation: AEE83054.1. CP002687 Genomic DNA. Translation: AEE83055.1.
IPI	IPI00522863. IPI00540666.
PIR	T09342.
RefSeq	NP_192920.3. NM_117252.5. NP_849539.1. NM_179208.2.
UniGene	At.70227.
3D structure databases
ProteinModelPortal	Q9T051.
SMR	Q9T051. Positions 34-186.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9T051.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G11830.2; AT4G11830.2; AT4G11830.
GeneID	826789.
GenomeReviews	Gene locus AT4G11830 in contig CT486007_GR.
KEGG	ath:AT4G11830.
NMPDR	fig|3702.1.peg.18853.
Organism-specific databases
TAIR	At4g11830.
Phylogenomic databases
eggNOG	KOG1329.
GeneTree	EPGT00070000028492.
HOGENOM	HBG748198.
InParanoid	Q9T051.
PhylomeDB	Q9T051.
ProtClustDB	CLSN2685536.
Enzyme and pathway databases
BRENDA	3.1.4.4. 399.
Gene expression databases
ArrayExpress	Q9T051.
Genevestigator	Q9T051.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR015679. PLipase_D. IPR001736. PLipase_D/transphosphatidylase. IPR024632. PLipase_D_C. IPR011402. PLipase_D_pln. [Graphical view]
KO	K01115.
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF12357. PLD_C. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDG2_ARATH

Accession

Primary (citable) accession number: Q9T051
Secondary accession number(s): Q3EA52, Q9XH77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program