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Protein

Protein NRT1/ PTR FAMILY 6.2

Gene

NPF6.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent nitrate transporter. Not involved in dipeptides transport.1 Publication

Kineticsi

  1. KM=2.4 mM for nitrate at -160 mV1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • nitrate assimilation Source: UniProtKB-KW
    • oligopeptide transport Source: InterPro
    • response to jasmonic acid Source: TAIR
    • response to wounding Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Nitrate assimilation, Symport, Transport

    Protein family/group databases

    TCDBi2.A.17.3.18. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 6.2
    Short name:
    AtNPF6.2
    Alternative name(s):
    Nitrate transporter 1.4
    Gene namesi
    Name:NPF6.2
    Synonyms:NRT1.4, NTP2
    Ordered Locus Names:At2g26690
    ORF Names:F18A8.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G26690.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
    Transmembranei74 – 94HelicalSequence analysisAdd BLAST21
    Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
    Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
    Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
    Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
    Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
    Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
    Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
    Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
    Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
    Transmembranei535 – 555HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Wider leaves resulting from increased cell expansion. Lower nitrate content of the petiole and midrib.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999611 – 577Protein NRT1/ PTR FAMILY 6.2Add BLAST577

    Proteomic databases

    PaxDbiQ9SZY4.

    Expressioni

    Tissue specificityi

    Expressed in shoots, leaves, flowers and siliques. Expressed in leaf petiole.2 Publications

    Inductioni

    Up-regulated in the shoots by nitrate, but no changes in the roots.1 Publication

    Gene expression databases

    GenevisibleiQ9SZY4. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G26690.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SZY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi183 – 191Phe-rich9

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ9SZY4.
    OMAiFDERDER.
    OrthoDBiEOG0936073F.
    PhylomeDBiQ9SZY4.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS01022. PTR2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SZY4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MESKGSWTVA DAVDYKGRPA DKSKTGGWIT AALILGIEVV ERLSTMGIAV
    60 70 80 90 100
    NLVTYLMETM HLPSSTSANI VTDFMGTSFL LCLLGGFLAD SFLGRFKTIG
    110 120 130 140 150
    IFSTIQALGT GALAVATKLP ELRPPTCHHG EACIPATAFQ MTILYVSLYL
    160 170 180 190 200
    IALGTGGLKS SISGFGSDQF DDKDPKEKAH MAFFFNRFFF FISMGTLLAV
    210 220 230 240 250
    TVLVYMQDEV GRSWAYGICT VSMAIAIVIF LCGTKRYRYK KSQGSPVVQI
    260 270 280 290 300
    FQVIAAAFRK RKMELPQSIV YLYEDNPEGI RIEHTDQFHL LDKAAIVAEG
    310 320 330 340 350
    DFEQTLDGVA IPNPWKLSSV TKVEEVKMMV RLLPIWATTI IFWTTYAQMI
    360 370 380 390 400
    TFSVEQASTM RRNIGSFKIP AGSLTVFFVA AILITLAVYD RAIMPFWKKW
    410 420 430 440 450
    KGKPGFSSLQ RIAIGLVLST AGMAAAALVE QKRLSVAKSS SQKTLPISVF
    460 470 480 490 500
    LLVPQFFLVG AGEAFIYTGQ LDFFITQSPK GMKTMSTGLF LTTLSLGFFV
    510 520 530 540 550
    SSFLVSIVKR VTSTSTDVGW LADNINHGRL DYFYWLLVIL SGINFVVYII
    560 570
    CALWFKPTKG KDSVEKENGK GFSVEDC
    Length:577
    Mass (Da):63,572
    Last modified:May 1, 2000 - v1
    Checksum:i05409F965933D0C4
    GO

    Sequence cautioni

    The sequence AAB95302 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti435S → C in AAM20651 (PubMed:14593172).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ011604 mRNA. Translation: CAB38705.1.
    AC003105 Genomic DNA. Translation: AAB95302.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07875.1.
    AY099800 mRNA. Translation: AAM20651.1.
    PIRiF84663.
    T52608.
    RefSeqiNP_850084.1. NM_179753.1.
    UniGeneiAt.383.

    Genome annotation databases

    EnsemblPlantsiAT2G26690.1; AT2G26690.1; AT2G26690.
    GeneIDi817210.
    GrameneiAT2G26690.1; AT2G26690.1; AT2G26690.
    KEGGiath:AT2G26690.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ011604 mRNA. Translation: CAB38705.1.
    AC003105 Genomic DNA. Translation: AAB95302.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07875.1.
    AY099800 mRNA. Translation: AAM20651.1.
    PIRiF84663.
    T52608.
    RefSeqiNP_850084.1. NM_179753.1.
    UniGeneiAt.383.

    3D structure databases

    ProteinModelPortaliQ9SZY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G26690.1.

    Protein family/group databases

    TCDBi2.A.17.3.18. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiQ9SZY4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G26690.1; AT2G26690.1; AT2G26690.
    GeneIDi817210.
    GrameneiAT2G26690.1; AT2G26690.1; AT2G26690.
    KEGGiath:AT2G26690.

    Organism-specific databases

    TAIRiAT2G26690.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ9SZY4.
    OMAiFDERDER.
    OrthoDBiEOG0936073F.
    PhylomeDBiQ9SZY4.

    Miscellaneous databases

    PROiQ9SZY4.

    Gene expression databases

    GenevisibleiQ9SZY4. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS01022. PTR2_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTR27_ARATH
    AccessioniPrimary (citable) accession number: Q9SZY4
    Secondary accession number(s): O48784, Q8LPH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: May 1, 2000
    Last modified: November 30, 2016
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.