Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SZX3

- ASSY_ARATH

UniProt

Q9SZX3 - ASSY_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Argininosuccinate synthase, chloroplastic

Gene

At4g24830

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei181 – 1811CitrullineBy similarity
Binding sitei186 – 1861CitrullineBy similarity
Binding sitei211 – 2111ATP; via amide nitrogenBy similarity
Binding sitei213 – 2131AspartateBy similarity
Binding sitei217 – 2171AspartateBy similarity
Binding sitei217 – 2171CitrullineBy similarity
Binding sitei218 – 2181AspartateBy similarity
Binding sitei221 – 2211CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei279 – 2791CitrullineBy similarity
Binding sitei355 – 3551CitrullineBy similarity
Binding sitei367 – 3671CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi102 – 1109ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G24830-MONOMER.
ARA:GQT-28-MONOMER.
ReactomeiREACT_253032. Urea cycle.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase, chloroplastic (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Ordered Locus Names:At4g24830
ORF Names:F6I7.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G24830.

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373ChloroplastSequence AnalysisAdd
BLAST
Chaini74 – 494421Argininosuccinate synthase, chloroplasticPRO_0000002412Add
BLAST

Proteomic databases

PaxDbiQ9SZX3.
PRIDEiQ9SZX3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SZX3. baseline and differential.
GenevestigatoriQ9SZX3.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi13875. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9SZX3.
SMRiQ9SZX3. Positions 98-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ9SZX3.
KOiK01940.
OMAiAPPEEAY.
PhylomeDBiQ9SZX3.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SZX3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEISATSFP SSSSSALVIR SSHNGSLKCQ NVAVPKTTSQ FQELSLKRSQ
60 70 80 90 100
LVGNAVVTGH VTGSRSCKNQ AIRAVLSGDG TALTTDSKEA GLRGKLKKVV
110 120 130 140 150
LAYSGGLDTS VIVPWLKENY GCEVVCFTAD VGQGIKELEG LEQKAKASGA
160 170 180 190 200
SQLVVKDLTE EFVKDFIFPC LRAGAIYERK YLLGTSMARP VIAKAMVDVA
210 220 230 240 250
AEVGADAVAH GCTGKGNDQV RFELTFFSLN PELKVVAPWR EWEIQGREDA
260 270 280 290 300
IEYAKKHNVP VPVTKKSIYS RDRNLWHLSH EGDLLEDPAN EPKKDMYMMS
310 320 330 340 350
VDPEDAPDQP EYIEIGIESG LPVALNGKAL SPATLLAELN TIGGKHGIGR
360 370 380 390 400
IDMVENRLVG MKSRGVYETP GGTILFAAVQ ELESLTLDRE SIQVKDTLAL
410 420 430 440 450
KYAEMVYAGR WFDPLRESMD AFMEKITETT TGSVTLKLYK GSVSVTGRQS
460 470 480 490
PNSLYRQDIS SFEGSEIYNQ ADAAGFIRLY GLPMKIRAML KKIS
Length:494
Mass (Da):53,846
Last modified:October 25, 2005 - v3
Checksum:i926F8D01AF32F052
GO

Sequence cautioni

The sequence CAB41123.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79393.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049657 Genomic DNA. Translation: CAB41123.1. Sequence problems.
AL161562 Genomic DNA. Translation: CAB79393.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84968.1.
AY065252 mRNA. Translation: AAL38728.1.
AY091319 mRNA. Translation: AAM14258.1.
PIRiT06667.
RefSeqiNP_194214.2. NM_118616.4. [Q9SZX3-1]
UniGeneiAt.3133.

Genome annotation databases

EnsemblPlantsiAT4G24830.1; AT4G24830.1; AT4G24830. [Q9SZX3-1]
GeneIDi828586.
KEGGiath:AT4G24830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049657 Genomic DNA. Translation: CAB41123.1 . Sequence problems.
AL161562 Genomic DNA. Translation: CAB79393.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84968.1 .
AY065252 mRNA. Translation: AAL38728.1 .
AY091319 mRNA. Translation: AAM14258.1 .
PIRi T06667.
RefSeqi NP_194214.2. NM_118616.4. [Q9SZX3-1 ]
UniGenei At.3133.

3D structure databases

ProteinModelPortali Q9SZX3.
SMRi Q9SZX3. Positions 98-492.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 13875. 1 interaction.

Proteomic databases

PaxDbi Q9SZX3.
PRIDEi Q9SZX3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G24830.1 ; AT4G24830.1 ; AT4G24830 . [Q9SZX3-1 ]
GeneIDi 828586.
KEGGi ath:AT4G24830.

Organism-specific databases

TAIRi AT4G24830.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
InParanoidi Q9SZX3.
KOi K01940.
OMAi APPEEAY.
PhylomeDBi Q9SZX3.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
BioCyci ARA:AT4G24830-MONOMER.
ARA:GQT-28-MONOMER.
Reactomei REACT_253032. Urea cycle.

Miscellaneous databases

PROi Q9SZX3.

Gene expression databases

ExpressionAtlasi Q9SZX3. baseline and differential.
Genevestigatori Q9SZX3.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiASSY_ARATH
AccessioniPrimary (citable) accession number: Q9SZX3
Secondary accession number(s): Q8VZ47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 25, 2005
Last modified: November 26, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3