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Q9SZX3

- ASSY_ARATH

UniProt

Q9SZX3 - ASSY_ARATH

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Protein
Argininosuccinate synthase, chloroplastic
Gene
At4g24830, F6I7.40
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei181 – 1811Citrulline By similarity
Binding sitei186 – 1861Citrulline By similarity
Binding sitei211 – 2111ATP; via amide nitrogen By similarity
Binding sitei213 – 2131Aspartate By similarity
Binding sitei217 – 2171Aspartate By similarity
Binding sitei217 – 2171Citrulline By similarity
Binding sitei218 – 2181Aspartate By similarity
Binding sitei221 – 2211Citrulline By similarity
Binding sitei270 – 2701Citrulline By similarity
Binding sitei279 – 2791Citrulline By similarity
Binding sitei355 – 3551Citrulline By similarity
Binding sitei367 – 3671Citrulline By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi102 – 1109ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. argininosuccinate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G24830-MONOMER.
ARA:GQT-28-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase, chloroplastic (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Ordered Locus Names:At4g24830
ORF Names:F6I7.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G24830.

Subcellular locationi

Plastidchloroplast Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373Chloroplast Reviewed prediction
Add
BLAST
Chaini74 – 494421Argininosuccinate synthase, chloroplasticUniRule annotation
PRO_0000002412Add
BLAST

Proteomic databases

PaxDbiQ9SZX3.
PRIDEiQ9SZX3.

Expressioni

Gene expression databases

ArrayExpressiQ9SZX3.
GenevestigatoriQ9SZX3.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi13875. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9SZX3.
SMRiQ9SZX3. Positions 98-492.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ9SZX3.
KOiK01940.
OMAiAPPEEAY.
PhylomeDBiQ9SZX3.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SZX3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEISATSFP SSSSSALVIR SSHNGSLKCQ NVAVPKTTSQ FQELSLKRSQ    50
LVGNAVVTGH VTGSRSCKNQ AIRAVLSGDG TALTTDSKEA GLRGKLKKVV 100
LAYSGGLDTS VIVPWLKENY GCEVVCFTAD VGQGIKELEG LEQKAKASGA 150
SQLVVKDLTE EFVKDFIFPC LRAGAIYERK YLLGTSMARP VIAKAMVDVA 200
AEVGADAVAH GCTGKGNDQV RFELTFFSLN PELKVVAPWR EWEIQGREDA 250
IEYAKKHNVP VPVTKKSIYS RDRNLWHLSH EGDLLEDPAN EPKKDMYMMS 300
VDPEDAPDQP EYIEIGIESG LPVALNGKAL SPATLLAELN TIGGKHGIGR 350
IDMVENRLVG MKSRGVYETP GGTILFAAVQ ELESLTLDRE SIQVKDTLAL 400
KYAEMVYAGR WFDPLRESMD AFMEKITETT TGSVTLKLYK GSVSVTGRQS 450
PNSLYRQDIS SFEGSEIYNQ ADAAGFIRLY GLPMKIRAML KKIS 494
Length:494
Mass (Da):53,846
Last modified:October 25, 2005 - v3
Checksum:i926F8D01AF32F052
GO

Sequence cautioni

The sequence CAB41123.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB79393.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049657 Genomic DNA. Translation: CAB41123.1. Sequence problems.
AL161562 Genomic DNA. Translation: CAB79393.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84968.1.
AY065252 mRNA. Translation: AAL38728.1.
AY091319 mRNA. Translation: AAM14258.1.
PIRiT06667.
RefSeqiNP_194214.2. NM_118616.4. [Q9SZX3-1]
UniGeneiAt.3133.

Genome annotation databases

EnsemblPlantsiAT4G24830.1; AT4G24830.1; AT4G24830. [Q9SZX3-1]
GeneIDi828586.
KEGGiath:AT4G24830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049657 Genomic DNA. Translation: CAB41123.1 . Sequence problems.
AL161562 Genomic DNA. Translation: CAB79393.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84968.1 .
AY065252 mRNA. Translation: AAL38728.1 .
AY091319 mRNA. Translation: AAM14258.1 .
PIRi T06667.
RefSeqi NP_194214.2. NM_118616.4. [Q9SZX3-1 ]
UniGenei At.3133.

3D structure databases

ProteinModelPortali Q9SZX3.
SMRi Q9SZX3. Positions 98-492.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 13875. 1 interaction.

Proteomic databases

PaxDbi Q9SZX3.
PRIDEi Q9SZX3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G24830.1 ; AT4G24830.1 ; AT4G24830 . [Q9SZX3-1 ]
GeneIDi 828586.
KEGGi ath:AT4G24830.

Organism-specific databases

TAIRi AT4G24830.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
InParanoidi Q9SZX3.
KOi K01940.
OMAi APPEEAY.
PhylomeDBi Q9SZX3.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
BioCyci ARA:AT4G24830-MONOMER.
ARA:GQT-28-MONOMER.

Miscellaneous databases

PROi Q9SZX3.

Gene expression databases

ArrayExpressi Q9SZX3.
Genevestigatori Q9SZX3.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiASSY_ARATH
AccessioniPrimary (citable) accession number: Q9SZX3
Secondary accession number(s): Q8VZ47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 25, 2005
Last modified: May 14, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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