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Protein

Argininosuccinate synthase, chloroplastic

Gene

At4g24830

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase, chloroplastic (At4g24830)
  3. Argininosuccinate lyase, chloroplastic (At5g10920)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei181CitrullineBy similarity1
Binding sitei186CitrullineBy similarity1
Binding sitei211ATP; via amide nitrogenBy similarity1
Binding sitei213AspartateBy similarity1
Binding sitei217AspartateBy similarity1
Binding sitei217CitrullineBy similarity1
Binding sitei218AspartateBy similarity1
Binding sitei221CitrullineBy similarity1
Binding sitei270CitrullineBy similarity1
Binding sitei279CitrullineBy similarity1
Binding sitei355CitrullineBy similarity1
Binding sitei367CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 110ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G24830-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase, chloroplastic (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Ordered Locus Names:At4g24830
ORF Names:F6I7.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G24830.
TAIRilocus:2126803. AT4G24830.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 73ChloroplastSequence analysisCombined sourcesAdd BLAST73
ChainiPRO_000000241274 – 494Argininosuccinate synthase, chloroplasticAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SZX3.
PRIDEiQ9SZX3.

PTM databases

iPTMnetiQ9SZX3.
SwissPalmiQ9SZX3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SZX3. baseline and differential.
GenevisibleiQ9SZX3. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi13875. 1 interactor.
STRINGi3702.AT4G24830.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SZX3.
SMRiQ9SZX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
InParanoidiQ9SZX3.
KOiK01940.
OMAiGVGRIDM.
OrthoDBiEOG093607A2.
PhylomeDBiQ9SZX3.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SZX3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEISATSFP SSSSSALVIR SSHNGSLKCQ NVAVPKTTSQ FQELSLKRSQ
60 70 80 90 100
LVGNAVVTGH VTGSRSCKNQ AIRAVLSGDG TALTTDSKEA GLRGKLKKVV
110 120 130 140 150
LAYSGGLDTS VIVPWLKENY GCEVVCFTAD VGQGIKELEG LEQKAKASGA
160 170 180 190 200
SQLVVKDLTE EFVKDFIFPC LRAGAIYERK YLLGTSMARP VIAKAMVDVA
210 220 230 240 250
AEVGADAVAH GCTGKGNDQV RFELTFFSLN PELKVVAPWR EWEIQGREDA
260 270 280 290 300
IEYAKKHNVP VPVTKKSIYS RDRNLWHLSH EGDLLEDPAN EPKKDMYMMS
310 320 330 340 350
VDPEDAPDQP EYIEIGIESG LPVALNGKAL SPATLLAELN TIGGKHGIGR
360 370 380 390 400
IDMVENRLVG MKSRGVYETP GGTILFAAVQ ELESLTLDRE SIQVKDTLAL
410 420 430 440 450
KYAEMVYAGR WFDPLRESMD AFMEKITETT TGSVTLKLYK GSVSVTGRQS
460 470 480 490
PNSLYRQDIS SFEGSEIYNQ ADAAGFIRLY GLPMKIRAML KKIS
Length:494
Mass (Da):53,846
Last modified:October 25, 2005 - v3
Checksum:i926F8D01AF32F052
GO

Sequence cautioni

The sequence CAB41123 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79393 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049657 Genomic DNA. Translation: CAB41123.1. Sequence problems.
AL161562 Genomic DNA. Translation: CAB79393.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84968.1.
AY065252 mRNA. Translation: AAL38728.1.
AY091319 mRNA. Translation: AAM14258.1.
PIRiT06667.
RefSeqiNP_194214.2. NM_118616.5. [Q9SZX3-1]
UniGeneiAt.3133.

Genome annotation databases

EnsemblPlantsiAT4G24830.1; AT4G24830.1; AT4G24830. [Q9SZX3-1]
GeneIDi828586.
GrameneiAT4G24830.1; AT4G24830.1; AT4G24830.
KEGGiath:AT4G24830.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASSY_ARATH
AccessioniPrimary (citable) accession number: Q9SZX3
Secondary accession number(s): Q8VZ47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 25, 2005
Last modified: October 25, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families