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Q9SZX3

- ASSY_ARATH

UniProt

Q9SZX3 - ASSY_ARATH

Protein

Argininosuccinate synthase, chloroplastic

Gene

At4g24830

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei129 – 1291ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei181 – 1811CitrullineBy similarity
    Binding sitei186 – 1861CitrullineBy similarity
    Binding sitei211 – 2111ATP; via amide nitrogenBy similarity
    Binding sitei213 – 2131AspartateBy similarity
    Binding sitei217 – 2171AspartateBy similarity
    Binding sitei217 – 2171CitrullineBy similarity
    Binding sitei218 – 2181AspartateBy similarity
    Binding sitei221 – 2211CitrullineBy similarity
    Binding sitei270 – 2701CitrullineBy similarity
    Binding sitei279 – 2791CitrullineBy similarity
    Binding sitei355 – 3551CitrullineBy similarity
    Binding sitei367 – 3671CitrullineBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi102 – 1109ATPBy similarity

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G24830-MONOMER.
    ARA:GQT-28-MONOMER.
    UniPathwayiUPA00068; UER00113.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthase, chloroplastic (EC:6.3.4.5)
    Alternative name(s):
    Citrulline--aspartate ligase
    Gene namesi
    Ordered Locus Names:At4g24830
    ORF Names:F6I7.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G24830.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7373ChloroplastSequence AnalysisAdd
    BLAST
    Chaini74 – 494421Argininosuccinate synthase, chloroplasticPRO_0000002412Add
    BLAST

    Proteomic databases

    PaxDbiQ9SZX3.
    PRIDEiQ9SZX3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9SZX3.
    GenevestigatoriQ9SZX3.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi13875. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SZX3.
    SMRiQ9SZX3. Positions 98-492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0137.
    HOGENOMiHOG000230093.
    InParanoidiQ9SZX3.
    KOiK01940.
    OMAiAPPEEAY.
    PhylomeDBiQ9SZX3.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SZX3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEISATSFP SSSSSALVIR SSHNGSLKCQ NVAVPKTTSQ FQELSLKRSQ    50
    LVGNAVVTGH VTGSRSCKNQ AIRAVLSGDG TALTTDSKEA GLRGKLKKVV 100
    LAYSGGLDTS VIVPWLKENY GCEVVCFTAD VGQGIKELEG LEQKAKASGA 150
    SQLVVKDLTE EFVKDFIFPC LRAGAIYERK YLLGTSMARP VIAKAMVDVA 200
    AEVGADAVAH GCTGKGNDQV RFELTFFSLN PELKVVAPWR EWEIQGREDA 250
    IEYAKKHNVP VPVTKKSIYS RDRNLWHLSH EGDLLEDPAN EPKKDMYMMS 300
    VDPEDAPDQP EYIEIGIESG LPVALNGKAL SPATLLAELN TIGGKHGIGR 350
    IDMVENRLVG MKSRGVYETP GGTILFAAVQ ELESLTLDRE SIQVKDTLAL 400
    KYAEMVYAGR WFDPLRESMD AFMEKITETT TGSVTLKLYK GSVSVTGRQS 450
    PNSLYRQDIS SFEGSEIYNQ ADAAGFIRLY GLPMKIRAML KKIS 494
    Length:494
    Mass (Da):53,846
    Last modified:October 25, 2005 - v3
    Checksum:i926F8D01AF32F052
    GO

    Sequence cautioni

    The sequence CAB41123.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB79393.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049657 Genomic DNA. Translation: CAB41123.1. Sequence problems.
    AL161562 Genomic DNA. Translation: CAB79393.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84968.1.
    AY065252 mRNA. Translation: AAL38728.1.
    AY091319 mRNA. Translation: AAM14258.1.
    PIRiT06667.
    RefSeqiNP_194214.2. NM_118616.4. [Q9SZX3-1]
    UniGeneiAt.3133.

    Genome annotation databases

    EnsemblPlantsiAT4G24830.1; AT4G24830.1; AT4G24830. [Q9SZX3-1]
    GeneIDi828586.
    KEGGiath:AT4G24830.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049657 Genomic DNA. Translation: CAB41123.1 . Sequence problems.
    AL161562 Genomic DNA. Translation: CAB79393.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84968.1 .
    AY065252 mRNA. Translation: AAL38728.1 .
    AY091319 mRNA. Translation: AAM14258.1 .
    PIRi T06667.
    RefSeqi NP_194214.2. NM_118616.4. [Q9SZX3-1 ]
    UniGenei At.3133.

    3D structure databases

    ProteinModelPortali Q9SZX3.
    SMRi Q9SZX3. Positions 98-492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13875. 1 interaction.

    Proteomic databases

    PaxDbi Q9SZX3.
    PRIDEi Q9SZX3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G24830.1 ; AT4G24830.1 ; AT4G24830 . [Q9SZX3-1 ]
    GeneIDi 828586.
    KEGGi ath:AT4G24830.

    Organism-specific databases

    TAIRi AT4G24830.

    Phylogenomic databases

    eggNOGi COG0137.
    HOGENOMi HOG000230093.
    InParanoidi Q9SZX3.
    KOi K01940.
    OMAi APPEEAY.
    PhylomeDBi Q9SZX3.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .
    BioCyci ARA:AT4G24830-MONOMER.
    ARA:GQT-28-MONOMER.

    Miscellaneous databases

    PROi Q9SZX3.

    Gene expression databases

    ArrayExpressi Q9SZX3.
    Genevestigatori Q9SZX3.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiASSY_ARATH
    AccessioniPrimary (citable) accession number: Q9SZX3
    Secondary accession number(s): Q8VZ47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3