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Protein

Cadmium/zinc-transporting ATPase HMA2

Gene

HMA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in zinc transport and homeostasis. Could also be involved in cadmium detoxification.1 Publication

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei391 – 39114-aspartylphosphate intermediateBy similarity
Metal bindingi592 – 5921MagnesiumPROSITE-ProRule annotation
Metal bindingi596 – 5961MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • zinc ion homeostasis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G30110-MONOMER.
BRENDAi3.6.3.5. 399.

Protein family/group databases

TCDBi3.A.3.6.7. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cadmium/zinc-transporting ATPase HMA2 (EC:3.6.3.3, EC:3.6.3.5)
Alternative name(s):
Cadmium/zinc-transporting ATPase 3
Protein HEAVY METAL ATPASE 2
Gene namesi
Name:HMA2
Ordered Locus Names:At4g30110
ORF Names:F6G3.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8383CytoplasmicSequence analysisAdd
BLAST
Transmembranei84 – 10522HelicalSequence analysisAdd
BLAST
Topological domaini106 – 1083ExtracellularSequence analysis
Transmembranei109 – 12820HelicalSequence analysisAdd
BLAST
Topological domaini129 – 1357CytoplasmicSequence analysis
Transmembranei136 – 15621HelicalSequence analysisAdd
BLAST
Topological domaini157 – 1571ExtracellularSequence analysis
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Topological domaini179 – 304126CytoplasmicSequence analysisAdd
BLAST
Transmembranei305 – 32723HelicalSequence analysisAdd
BLAST
Topological domaini328 – 3358ExtracellularSequence analysis
Transmembranei336 – 35318HelicalSequence analysisAdd
BLAST
Topological domaini354 – 647294CytoplasmicSequence analysisAdd
BLAST
Transmembranei648 – 66720HelicalSequence analysisAdd
BLAST
Topological domaini668 – 6714ExtracellularSequence analysis
Transmembranei672 – 69120HelicalSequence analysisAdd
BLAST
Topological domaini692 – 951260CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 951951Cadmium/zinc-transporting ATPase HMA2PRO_0000046400Add
BLAST

Proteomic databases

PaxDbiQ9SZW4.
PRIDEiQ9SZW4.

PTM databases

iPTMnetiQ9SZW4.

Expressioni

Tissue specificityi

Predominantly expressed in the vascular tissues of roots, stems, and leaves. Also detected in developing anthers.1 Publication

Gene expression databases

GenevisibleiQ9SZW4. AT.

Interactioni

Protein-protein interaction databases

BioGridi14421. 1 interaction.
STRINGi3702.AT4G30110.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SZW4.
SMRiQ9SZW4. Positions 6-694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 7467HMAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi708 – 7125Poly-Ser

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiQ9SZW4.
KOiK01534.
OMAiTEFQIYP.
PhylomeDBiQ9SZW4.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SZW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKKMTKSY FDVLGICCTS EVPLIENILN SMDGVKEFSV IVPSRTVIVV
60 70 80 90 100
HDTLILSQFQ IVKALNQAQL EANVRVTGET NFKNKWPSPF AVVSGILLLL
110 120 130 140 150
SFFKYLYSPF RWLAVAAVVA GIYPILAKAV ASLARFRIDI NILVVVTVGA
160 170 180 190 200
TIGMQDYTEA AVVVFLFTIA EWLQSRASYK ASAVMQSLMS LAPQKAVIAE
210 220 230 240 250
TGEEVEVDEL KTNTVIAVKA GETIPIDGVV VDGNCEVDEK TLTGEAFPVP
260 270 280 290 300
KLKDSTVWAG TINLNGYITV NTTALAEDCV VAKMAKLVEE AQNSKTETQR
310 320 330 340 350
FIDKCSKYYT PAIILISICF VAIPFALKVH NLKHWVHLAL VVLVSACPCG
360 370 380 390 400
LILSTPVATF CALTKAATSG LLIKGADYLE TLAKIKIVAF DKTGTITRGE
410 420 430 440 450
FIVMDFQSLS EDISLQSLLY WVSSTESKSS HPMAAAVVDY ARSVSVEPKP
460 470 480 490 500
EAVEDYQNFP GEGIYGKIDG KEVYIGNKRI ASRAGCLSVP DIDVDTKGGK
510 520 530 540 550
TIGYVYVGET LAGVFNLSDA CRSGVAQAMK ELKSLGIKIA MLTGDNHAAA
560 570 580 590 600
MHAQEQLGNA MDIVRAELLP EDKSEIIKQL KREEGPTAMV GDGLNDAPAL
610 620 630 640 650
ATADIGISMG VSGSALATET GNIILMSNDI RRIPQAIKLA KRAKRKVVEN
660 670 680 690 700
VVISITMKGA ILALAFAGHP LIWAAVLADV GTCLLVILNS MLLLSDKHKT
710 720 730 740 750
GNKCYRESSS SSVLIAEKLE GDAAGDMEAG LLPKISDKHC KPGCCGTKTQ
760 770 780 790 800
EKAMKPAKAS SDHSHSGCCE TKQKDNVTVV KKSCCAEPVD LGHGHDSGCC
810 820 830 840 850
GDKSQQPHQH EVQVQQSCHN KPSGLDSGCC GGKSQQPHQH ELQQSCHDKP
860 870 880 890 900
SGLDIGTGPK HEGSSTLVNL EGDAKEELKV LVNGFCSSPA DLAITSLKVK
910 920 930 940 950
SDSHCKSNCS SRERCHHGSN CCRSYAKESC SHDHHHTRAH GVGTLKEIVI

E
Length:951
Mass (Da):101,990
Last modified:May 1, 2000 - v1
Checksum:i82E51995034FCF9D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti459 – 4591F → L in AAR10767 (Ref. 1) Curated
Sequence conflicti459 – 4591F → L in AAL14248 (PubMed:15100400).Curated
Sequence conflicti759 – 7591A → T in AAR10767 (Ref. 1) Curated
Sequence conflicti759 – 7591A → T in AAL14248 (PubMed:15100400).Curated
Sequence conflicti776 – 7761N → K in AAR10767 (Ref. 1) Curated
Sequence conflicti776 – 7761N → K in AAL14248 (PubMed:15100400).Curated
Sequence conflicti783 – 7831S → N in AAR10767 (Ref. 1) Curated
Sequence conflicti783 – 7831S → N in AAL14248 (PubMed:15100400).Curated
Sequence conflicti885 – 8851F → C in AAR10767 (Ref. 1) Curated
Sequence conflicti885 – 8851F → C in AAL14248 (PubMed:15100400).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY054390 mRNA. Translation: AAL14248.1.
AY434728 mRNA. Translation: AAR10767.1.
AL078464 Genomic DNA. Translation: CAB43846.1.
AL161576 Genomic DNA. Translation: CAB81004.1.
CP002687 Genomic DNA. Translation: AEE85722.1.
PIRiT08987.
RefSeqiNP_194740.1. NM_119157.3.
UniGeneiAt.31858.

Genome annotation databases

EnsemblPlantsiAT4G30110.1; AT4G30110.1; AT4G30110.
GeneIDi829134.
GrameneiAT4G30110.1; AT4G30110.1; AT4G30110.
KEGGiath:AT4G30110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY054390 mRNA. Translation: AAL14248.1.
AY434728 mRNA. Translation: AAR10767.1.
AL078464 Genomic DNA. Translation: CAB43846.1.
AL161576 Genomic DNA. Translation: CAB81004.1.
CP002687 Genomic DNA. Translation: AEE85722.1.
PIRiT08987.
RefSeqiNP_194740.1. NM_119157.3.
UniGeneiAt.31858.

3D structure databases

ProteinModelPortaliQ9SZW4.
SMRiQ9SZW4. Positions 6-694.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14421. 1 interaction.
STRINGi3702.AT4G30110.1.

Protein family/group databases

TCDBi3.A.3.6.7. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiQ9SZW4.

Proteomic databases

PaxDbiQ9SZW4.
PRIDEiQ9SZW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30110.1; AT4G30110.1; AT4G30110.
GeneIDi829134.
GrameneiAT4G30110.1; AT4G30110.1; AT4G30110.
KEGGiath:AT4G30110.

Organism-specific databases

TAIRiAT4G30110.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiQ9SZW4.
KOiK01534.
OMAiTEFQIYP.
PhylomeDBiQ9SZW4.

Enzyme and pathway databases

BioCyciARA:AT4G30110-MONOMER.
BRENDAi3.6.3.5. 399.

Miscellaneous databases

PROiQ9SZW4.

Gene expression databases

GenevisibleiQ9SZW4. AT.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AtHMA2, a putative heavy-metal P-type ATPase in Arabidopsis."
    Richaud P.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Wassilewskija.
    Tissue: Leaf.
  2. "P-type ATPase heavy metal transporters with roles in essential zinc homeostasis in Arabidopsis."
    Hussain D., Haydon M.J., Wang Y., Wong E., Sherson S.M., Young J., Camakaris J., Harper J.F., Cobbett C.S.
    Plant Cell 16:1327-1339(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiHMA2_ARATH
AccessioniPrimary (citable) accession number: Q9SZW4
Secondary accession number(s): Q8LPW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.