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Q9SZU7 (KAI2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable esterase KAI2
Alternative name(s):
Protein DWARF-14-like
Short name=Protein D14-like
Protein HYPOSENSITIVE TO LIGHT
Protein KARRIKIN INSENSITIVE 2
Gene names
Name:KAI2
Synonyms:D14L, HTL
Ordered Locus Names:At4g37470
ORF Names:F6G17.120
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Ref.4 Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus. Cytoplasmcytosol. Note: Weak expression in the cytosol. Ref.4

Tissue specificity

In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips. Ref.4

Induction

By red light. Ref.6

Disruption phenotype

Increased seed dormancy. Long hypocotyl phenotype under red, far-red, and blue light. Ref.4 Ref.5

Sequence similarities

Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processde-etiolation

Inferred from mutant phenotype Ref.4. Source: TAIR

photomorphogenesis

Inferred from mutant phenotype Ref.4. Source: TAIR

response to karrikin

Inferred from expression pattern PubMed 20351290. Source: TAIR

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Probable esterase KAI2
PRO_0000422056

Sites

Active site951Nucleophile By similarity
Active site2171 By similarity
Active site2461 By similarity

Experimental info

Mutagenesis1331G → E in kai2-1; loss of function. Ref.5 Ref.7

Secondary structure

............................................. 270
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9SZU7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: DE49CA592174FFBE

FASTA27029,791
        10         20         30         40         50         60 
MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD NMGAGTTNPD 

        70         80         90        100        110        120 
YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI GVLASLNRPD LFSKIVMISA 

       130        140        150        160        170        180 
SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN 

       190        200        210        220        230        240 
MRPDIALSVG QTIFQSDMRQ ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV 

       250        260        270 
IPSDGHLPQL SSPDSVIPVI LRHIRNDIAM 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation."
Sun X.D., Ni M.
Mol. Plant 4:116-126(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: cv. Landsberg erecta.
[5]"Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis."
Waters M.T., Nelson D.C., Scaffidi A., Flematti G.R., Sun Y.K., Dixon K.W., Smith S.M.
Development 139:1285-1295(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-133.
Strain: cv. Landsberg erecta.
[6]"KAI2- and MAX2-mediated responses to karrikins and strigolactones are largely independent of HY5 in Arabidopsis seedlings."
Waters M.T., Smith S.M.
Mol. Plant 6:63-75(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY LIGHT.
Strain: cv. Landsberg erecta.
[7]"Structures of D14 and D14L in the strigolactone and karrikin signaling pathways."
Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T.
Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), FUNCTION, MUTAGENESIS OF GLY-133.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL035601 Genomic DNA. Translation: CAB38214.1.
AL161591 Genomic DNA. Translation: CAB80412.1.
CP002687 Genomic DNA. Translation: AEE86798.1.
AY056190 mRNA. Translation: AAL07039.1.
AY091347 mRNA. Translation: AAM14286.1.
PIRT04741.
RefSeqNP_195463.1. NM_119911.3.
UniGeneAt.66602.
At.71877.
At.72976.
At.74782.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3W06X-ray1.15A1-270[»]
4HRXX-ray2.11A1-270[»]
4HRYX-ray1.50A1-270[»]
4HTAX-ray1.90A1-270[»]
4IH1X-ray1.55A1-270[»]
4JYMX-ray1.35A/B1-270[»]
4JYPX-ray1.30A/B1-270[»]
ProteinModelPortalQ9SZU7.
SMRQ9SZU7. Positions 2-268.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G37470.1-P.

Protein family/group databases

MEROPSS33.A29.

Proteomic databases

PaxDbQ9SZU7.
PRIDEQ9SZU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G37470.1; AT4G37470.1; AT4G37470.
GeneID829902.
KEGGath:AT4G37470.

Organism-specific databases

TAIRAT4G37470.

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000251386.
InParanoidQ9SZU7.
OMACADDIIA.
PhylomeDBQ9SZU7.

Gene expression databases

GenevestigatorQ9SZU7.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKAI2_ARATH
AccessionPrimary (citable) accession number: Q9SZU7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names