Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SZU7

- KAI2_ARATH

UniProt

Q9SZU7 - KAI2_ARATH

Protein

Probable esterase KAI2

Gene

KAI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951NucleophileBy similarity
    Active sitei217 – 2171By similarity
    Active sitei246 – 2461By similarity

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. de-etiolation Source: TAIR
    2. photomorphogenesis Source: TAIR
    3. response to karrikin Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Protein family/group databases

    MEROPSiS33.A29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable esterase KAI2
    Alternative name(s):
    Protein DWARF-14-like
    Short name:
    Protein D14-like
    Protein HYPOSENSITIVE TO LIGHT
    Protein KARRIKIN INSENSITIVE 2
    Gene namesi
    Name:KAI2
    Synonyms:D14L, HTL
    Ordered Locus Names:At4g37470
    ORF Names:F6G17.120
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G37470.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytosol 1 Publication
    Note: Weak expression in the cytosol.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Increased seed dormancy. Long hypocotyl phenotype under red, far-red, and blue light.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi133 – 1331G → E in kai2-1; loss of function. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Probable esterase KAI2PRO_0000422056Add
    BLAST

    Proteomic databases

    PaxDbiQ9SZU7.
    PRIDEiQ9SZU7.

    Expressioni

    Tissue specificityi

    In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips.1 Publication

    Inductioni

    By red light.1 Publication

    Gene expression databases

    GenevestigatoriQ9SZU7.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G37470.1-P.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 75
    Beta strandi11 – 144
    Beta strandi19 – 235
    Helixi30 – 334
    Turni34 – 363
    Helixi37 – 393
    Turni40 – 434
    Beta strandi44 – 485
    Helixi59 – 613
    Helixi66 – 683
    Helixi70 – 8314
    Beta strandi89 – 946
    Helixi96 – 10712
    Helixi109 – 1113
    Beta strandi112 – 1198
    Helixi136 – 14813
    Helixi150 – 16213
    Helixi169 – 18012
    Helixi183 – 19412
    Helixi199 – 2046
    Beta strandi209 – 2168
    Helixi222 – 23110
    Beta strandi236 – 24712
    Helixi248 – 2514
    Helixi253 – 26513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3W06X-ray1.15A1-270[»]
    4HRXX-ray2.11A1-270[»]
    4HRYX-ray1.50A1-270[»]
    4HTAX-ray1.90A1-270[»]
    4IH1X-ray1.55A1-270[»]
    4JYMX-ray1.35A/B1-270[»]
    4JYPX-ray1.30A/B1-270[»]
    ProteinModelPortaliQ9SZU7.
    SMRiQ9SZU7. Positions 2-268.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000251386.
    InParanoidiQ9SZU7.
    OMAiCADDIIA.
    PhylomeDBiQ9SZU7.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9SZU7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD    50
    NMGAGTTNPD YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI 100
    GVLASLNRPD LFSKIVMISA SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY 150
    KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN MRPDIALSVG QTIFQSDMRQ 200
    ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV IPSDGHLPQL 250
    SSPDSVIPVI LRHIRNDIAM 270
    Length:270
    Mass (Da):29,791
    Last modified:May 1, 2000 - v1
    Checksum:iDE49CA592174FFBE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035601 Genomic DNA. Translation: CAB38214.1.
    AL161591 Genomic DNA. Translation: CAB80412.1.
    CP002687 Genomic DNA. Translation: AEE86798.1.
    AY056190 mRNA. Translation: AAL07039.1.
    AY091347 mRNA. Translation: AAM14286.1.
    PIRiT04741.
    RefSeqiNP_195463.1. NM_119911.3.
    UniGeneiAt.66602.
    At.71877.
    At.72976.
    At.74782.

    Genome annotation databases

    EnsemblPlantsiAT4G37470.1; AT4G37470.1; AT4G37470.
    GeneIDi829902.
    KEGGiath:AT4G37470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035601 Genomic DNA. Translation: CAB38214.1 .
    AL161591 Genomic DNA. Translation: CAB80412.1 .
    CP002687 Genomic DNA. Translation: AEE86798.1 .
    AY056190 mRNA. Translation: AAL07039.1 .
    AY091347 mRNA. Translation: AAM14286.1 .
    PIRi T04741.
    RefSeqi NP_195463.1. NM_119911.3.
    UniGenei At.66602.
    At.71877.
    At.72976.
    At.74782.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3W06 X-ray 1.15 A 1-270 [» ]
    4HRX X-ray 2.11 A 1-270 [» ]
    4HRY X-ray 1.50 A 1-270 [» ]
    4HTA X-ray 1.90 A 1-270 [» ]
    4IH1 X-ray 1.55 A 1-270 [» ]
    4JYM X-ray 1.35 A/B 1-270 [» ]
    4JYP X-ray 1.30 A/B 1-270 [» ]
    ProteinModelPortali Q9SZU7.
    SMRi Q9SZU7. Positions 2-268.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G37470.1-P.

    Protein family/group databases

    MEROPSi S33.A29.

    Proteomic databases

    PaxDbi Q9SZU7.
    PRIDEi Q9SZU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G37470.1 ; AT4G37470.1 ; AT4G37470 .
    GeneIDi 829902.
    KEGGi ath:AT4G37470.

    Organism-specific databases

    TAIRi AT4G37470.

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000251386.
    InParanoidi Q9SZU7.
    OMAi CADDIIA.
    PhylomeDBi Q9SZU7.

    Gene expression databases

    Genevestigatori Q9SZU7.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation."
      Sun X.D., Ni M.
      Mol. Plant 4:116-126(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Landsberg erecta.
    5. "Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis."
      Waters M.T., Nelson D.C., Scaffidi A., Flematti G.R., Sun Y.K., Dixon K.W., Smith S.M.
      Development 139:1285-1295(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-133.
      Strain: cv. Landsberg erecta.
    6. "KAI2- and MAX2-mediated responses to karrikins and strigolactones are largely independent of HY5 in Arabidopsis seedlings."
      Waters M.T., Smith S.M.
      Mol. Plant 6:63-75(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY LIGHT.
      Strain: cv. Landsberg erecta.
    7. "Structures of D14 and D14L in the strigolactone and karrikin signaling pathways."
      Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T.
      Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), FUNCTION, MUTAGENESIS OF GLY-133.

    Entry informationi

    Entry nameiKAI2_ARATH
    AccessioniPrimary (citable) accession number: Q9SZU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3