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Protein

Probable esterase KAI2

Gene

KAI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones (PubMed:20864454, PubMed:22357928, PubMed:23142794, PubMed:23301669). Lacks detectable hydrolase activity against karrikin (PubMed:23613584). Karrikin binding induces a conformational change (PubMed:23613584).5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Nucleophile2 Publications1
Active sitei2172 Publications1
Active sitei2462 Publications1

GO - Molecular functioni

GO - Biological processi

  • de-etiolation Source: TAIR
  • photomorphogenesis Source: TAIR
  • response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

ESTHERiarath-KAI2.D14L. RsbQ-like.
MEROPSiS33.A29.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable esterase KAI2
Alternative name(s):
Protein DWARF-14-like
Short name:
Protein D14-like
Protein HYPOSENSITIVE TO LIGHT
Protein KARRIKIN INSENSITIVE 2
Gene namesi
Name:KAI2
Synonyms:D14L, HTL
Ordered Locus Names:At4g37470
ORF Names:F6G17.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G37470.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasmcytosol 1 Publication

  • Note: Weak expression in the cytosol.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Increased seed dormancy. Long hypocotyl phenotype under red, far-red, and blue light.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133G → E in kai2-1; loss of function. 2 Publications1
Mutagenesisi134F → A: Fivefold decreased affinity for karrikin. 1 Publication1
Mutagenesisi194F → A: Fivefold decreased affinity for karrikin. 1 Publication1
Mutagenesisi246H → A: Threefold decreased affinity for karrikin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004220561 – 270Probable esterase KAI2Add BLAST270

Proteomic databases

PaxDbiQ9SZU7.

Expressioni

Tissue specificityi

In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips.1 Publication

Inductioni

By red light.1 Publication

Gene expression databases

GenevisibleiQ9SZU7. AT.

Interactioni

Protein-protein interaction databases

BioGridi15183. 1 interactor.
STRINGi3702.AT4G37470.1.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 7Combined sources5
Beta strandi11 – 14Combined sources4
Beta strandi19 – 23Combined sources5
Helixi30 – 33Combined sources4
Turni34 – 36Combined sources3
Helixi37 – 39Combined sources3
Turni40 – 43Combined sources4
Beta strandi44 – 48Combined sources5
Helixi59 – 61Combined sources3
Helixi66 – 68Combined sources3
Helixi70 – 83Combined sources14
Beta strandi89 – 94Combined sources6
Helixi96 – 107Combined sources12
Helixi109 – 111Combined sources3
Beta strandi112 – 119Combined sources8
Helixi136 – 148Combined sources13
Helixi150 – 162Combined sources13
Helixi169 – 180Combined sources12
Helixi183 – 194Combined sources12
Helixi199 – 204Combined sources6
Beta strandi209 – 216Combined sources8
Helixi222 – 231Combined sources10
Beta strandi236 – 247Combined sources12
Helixi248 – 251Combined sources4
Helixi253 – 265Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W06X-ray1.15A1-270[»]
4HRXX-ray2.11A1-270[»]
4HRYX-ray1.50A1-270[»]
4HTAX-ray1.90A1-270[»]
4IH1X-ray1.55A1-270[»]
4JYMX-ray1.35A/B1-270[»]
4JYPX-ray1.30A/B1-270[»]
ProteinModelPortaliQ9SZU7.
SMRiQ9SZU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410IM0C. Eukaryota.
COG0596. LUCA.
HOGENOMiHOG000251386.
InParanoidiQ9SZU7.
OMAiMEKNYIG.
OrthoDBiEOG09360INU.
PhylomeDBiQ9SZU7.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SZU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD
60 70 80 90 100
NMGAGTTNPD YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI
110 120 130 140 150
GVLASLNRPD LFSKIVMISA SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY
160 170 180 190 200
KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN MRPDIALSVG QTIFQSDMRQ
210 220 230 240 250
ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV IPSDGHLPQL
260 270
SSPDSVIPVI LRHIRNDIAM
Length:270
Mass (Da):29,791
Last modified:May 1, 2000 - v1
Checksum:iDE49CA592174FFBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035601 Genomic DNA. Translation: CAB38214.1.
AL161591 Genomic DNA. Translation: CAB80412.1.
CP002687 Genomic DNA. Translation: AEE86798.1.
AY056190 mRNA. Translation: AAL07039.1.
AY091347 mRNA. Translation: AAM14286.1.
PIRiT04741.
RefSeqiNP_195463.1. NM_119911.4.
UniGeneiAt.66602.
At.71877.
At.72976.
At.74782.

Genome annotation databases

EnsemblPlantsiAT4G37470.1; AT4G37470.1; AT4G37470.
GeneIDi829902.
GrameneiAT4G37470.1; AT4G37470.1; AT4G37470.
KEGGiath:AT4G37470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035601 Genomic DNA. Translation: CAB38214.1.
AL161591 Genomic DNA. Translation: CAB80412.1.
CP002687 Genomic DNA. Translation: AEE86798.1.
AY056190 mRNA. Translation: AAL07039.1.
AY091347 mRNA. Translation: AAM14286.1.
PIRiT04741.
RefSeqiNP_195463.1. NM_119911.4.
UniGeneiAt.66602.
At.71877.
At.72976.
At.74782.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W06X-ray1.15A1-270[»]
4HRXX-ray2.11A1-270[»]
4HRYX-ray1.50A1-270[»]
4HTAX-ray1.90A1-270[»]
4IH1X-ray1.55A1-270[»]
4JYMX-ray1.35A/B1-270[»]
4JYPX-ray1.30A/B1-270[»]
ProteinModelPortaliQ9SZU7.
SMRiQ9SZU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15183. 1 interactor.
STRINGi3702.AT4G37470.1.

Protein family/group databases

ESTHERiarath-KAI2.D14L. RsbQ-like.
MEROPSiS33.A29.

Proteomic databases

PaxDbiQ9SZU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37470.1; AT4G37470.1; AT4G37470.
GeneIDi829902.
GrameneiAT4G37470.1; AT4G37470.1; AT4G37470.
KEGGiath:AT4G37470.

Organism-specific databases

TAIRiAT4G37470.

Phylogenomic databases

eggNOGiENOG410IM0C. Eukaryota.
COG0596. LUCA.
HOGENOMiHOG000251386.
InParanoidiQ9SZU7.
OMAiMEKNYIG.
OrthoDBiEOG09360INU.
PhylomeDBiQ9SZU7.

Miscellaneous databases

PROiQ9SZU7.

Gene expression databases

GenevisibleiQ9SZU7. AT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAI2_ARATH
AccessioniPrimary (citable) accession number: Q9SZU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.