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Q9SZU7

- KAI2_ARATH

UniProt

Q9SZU7 - KAI2_ARATH

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Protein

Probable esterase KAI2

Gene
KAI2, D14L, HTL, At4g37470, F6G17.120
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Nucleophile By similarity
Active sitei217 – 2171 By similarity
Active sitei246 – 2461 By similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. de-etiolation Source: TAIR
  2. photomorphogenesis Source: TAIR
  3. response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

MEROPSiS33.A29.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable esterase KAI2
Alternative name(s):
Protein DWARF-14-like
Short name:
Protein D14-like
Protein HYPOSENSITIVE TO LIGHT
Protein KARRIKIN INSENSITIVE 2
Gene namesi
Name:KAI2
Synonyms:D14L, HTL
Ordered Locus Names:At4g37470
ORF Names:F6G17.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G37470.

Subcellular locationi

Nucleus. Cytoplasmcytosol
Note: Weak expression in the cytosol.1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Increased seed dormancy. Long hypocotyl phenotype under red, far-red, and blue light.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi133 – 1331G → E in kai2-1; loss of function. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Probable esterase KAI2PRO_0000422056Add
BLAST

Proteomic databases

PaxDbiQ9SZU7.
PRIDEiQ9SZU7.

Expressioni

Tissue specificityi

In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips.1 Publication

Inductioni

By red light.1 Publication

Gene expression databases

GenevestigatoriQ9SZU7.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37470.1-P.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 75
Beta strandi11 – 144
Beta strandi19 – 235
Helixi30 – 334
Turni34 – 363
Helixi37 – 393
Turni40 – 434
Beta strandi44 – 485
Helixi59 – 613
Helixi66 – 683
Helixi70 – 8314
Beta strandi89 – 946
Helixi96 – 10712
Helixi109 – 1113
Beta strandi112 – 1198
Helixi136 – 14813
Helixi150 – 16213
Helixi169 – 18012
Helixi183 – 19412
Helixi199 – 2046
Beta strandi209 – 2168
Helixi222 – 23110
Beta strandi236 – 24712
Helixi248 – 2514
Helixi253 – 26513

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W06X-ray1.15A1-270[»]
4HRXX-ray2.11A1-270[»]
4HRYX-ray1.50A1-270[»]
4HTAX-ray1.90A1-270[»]
4IH1X-ray1.55A1-270[»]
4JYMX-ray1.35A/B1-270[»]
4JYPX-ray1.30A/B1-270[»]
ProteinModelPortaliQ9SZU7.
SMRiQ9SZU7. Positions 2-268.

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000251386.
InParanoidiQ9SZU7.
OMAiCADDIIA.
PhylomeDBiQ9SZU7.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SZU7-1 [UniParc]FASTAAdd to Basket

« Hide

MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD    50
NMGAGTTNPD YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI 100
GVLASLNRPD LFSKIVMISA SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY 150
KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN MRPDIALSVG QTIFQSDMRQ 200
ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV IPSDGHLPQL 250
SSPDSVIPVI LRHIRNDIAM 270
Length:270
Mass (Da):29,791
Last modified:May 1, 2000 - v1
Checksum:iDE49CA592174FFBE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL035601 Genomic DNA. Translation: CAB38214.1.
AL161591 Genomic DNA. Translation: CAB80412.1.
CP002687 Genomic DNA. Translation: AEE86798.1.
AY056190 mRNA. Translation: AAL07039.1.
AY091347 mRNA. Translation: AAM14286.1.
PIRiT04741.
RefSeqiNP_195463.1. NM_119911.3.
UniGeneiAt.66602.
At.71877.
At.72976.
At.74782.

Genome annotation databases

EnsemblPlantsiAT4G37470.1; AT4G37470.1; AT4G37470.
GeneIDi829902.
KEGGiath:AT4G37470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL035601 Genomic DNA. Translation: CAB38214.1 .
AL161591 Genomic DNA. Translation: CAB80412.1 .
CP002687 Genomic DNA. Translation: AEE86798.1 .
AY056190 mRNA. Translation: AAL07039.1 .
AY091347 mRNA. Translation: AAM14286.1 .
PIRi T04741.
RefSeqi NP_195463.1. NM_119911.3.
UniGenei At.66602.
At.71877.
At.72976.
At.74782.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3W06 X-ray 1.15 A 1-270 [» ]
4HRX X-ray 2.11 A 1-270 [» ]
4HRY X-ray 1.50 A 1-270 [» ]
4HTA X-ray 1.90 A 1-270 [» ]
4IH1 X-ray 1.55 A 1-270 [» ]
4JYM X-ray 1.35 A/B 1-270 [» ]
4JYP X-ray 1.30 A/B 1-270 [» ]
ProteinModelPortali Q9SZU7.
SMRi Q9SZU7. Positions 2-268.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G37470.1-P.

Protein family/group databases

MEROPSi S33.A29.

Proteomic databases

PaxDbi Q9SZU7.
PRIDEi Q9SZU7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G37470.1 ; AT4G37470.1 ; AT4G37470 .
GeneIDi 829902.
KEGGi ath:AT4G37470.

Organism-specific databases

TAIRi AT4G37470.

Phylogenomic databases

eggNOGi COG0596.
HOGENOMi HOG000251386.
InParanoidi Q9SZU7.
OMAi CADDIIA.
PhylomeDBi Q9SZU7.

Gene expression databases

Genevestigatori Q9SZU7.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation."
    Sun X.D., Ni M.
    Mol. Plant 4:116-126(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  5. "Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis."
    Waters M.T., Nelson D.C., Scaffidi A., Flematti G.R., Sun Y.K., Dixon K.W., Smith S.M.
    Development 139:1285-1295(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-133.
    Strain: cv. Landsberg erecta.
  6. "KAI2- and MAX2-mediated responses to karrikins and strigolactones are largely independent of HY5 in Arabidopsis seedlings."
    Waters M.T., Smith S.M.
    Mol. Plant 6:63-75(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT.
    Strain: cv. Landsberg erecta.
  7. "Structures of D14 and D14L in the strigolactone and karrikin signaling pathways."
    Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T.
    Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), FUNCTION, MUTAGENESIS OF GLY-133.

Entry informationi

Entry nameiKAI2_ARATH
AccessioniPrimary (citable) accession number: Q9SZU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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