Q9SZU7 (KAI2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable esterase KAI2

Alternative name(s):
Protein DWARF-14-like
Short name=Protein D14-like
Protein HYPOSENSITIVE TO LIGHT
Protein KARRIKIN INSENSITIVE 2

Gene names

Name:	KAI2
Synonyms:	D14L, HTL
Ordered Locus Names:	At4g37470
ORF Names:	F6G17.120

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Ref.4 Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Cytoplasm › cytosol. Note: Weak expression in the cytosol. Ref.4
Tissue specificity	In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips. Ref.4
Induction	By red light. Ref.6
Disruption phenotype	Increased seed dormancy. Long hypocotyl phenotype under red, far-red, and blue light. Ref.4 Ref.5
Sequence similarities	Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	de-etiolation Inferred from mutant phenotype Ref.4. Source: TAIR response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Probable esterase KAI2		PRO_0000422056
Sites
	Active site	95	1	Nucleophile By similarity
	Active site	217	1	By similarity
	Active site	246	1	By similarity
Experimental info
	Mutagenesis	133	1	G → E in kai2-1; loss of function. Ref.5 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9SZU7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DE49CA592174FFBE	FASTA	270	29,791
	10 20 30 40 50 60 MGVVEEAHNV KVIGSGEATI VLGHGFGTDQ SVWKHLVPHL VDDYRVVLYD NMGAGTTNPD 70 80 90 100 110 120 YFDFDRYSNL EGYSFDLIAI LEDLKIESCI FVGHSVSAMI GVLASLNRPD LFSKIVMISA 130 140 150 160 170 180 SPRYVNDVDY QGGFEQEDLN QLFEAIRSNY KAWCLGFAPL AVGGDMDSIA VQEFSRTLFN 190 200 210 220 230 240 MRPDIALSVG QTIFQSDMRQ ILPFVTVPCH ILQSVKDLAV PVVVSEYLHA NLGCESVVEV 250 260 270 IPSDGHLPQL SSPDSVIPVI LRHIRNDIAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation." Sun X.D., Ni M. Mol. Plant 4:116-126(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: cv. Landsberg erecta.
[5]	"Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis." Waters M.T., Nelson D.C., Scaffidi A., Flematti G.R., Sun Y.K., Dixon K.W., Smith S.M. Development 139:1285-1295(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-133. Strain: cv. Landsberg erecta.
[6]	"KAI2- and MAX2-mediated responses to karrikins and strigolactones are largely independent of HY5 in Arabidopsis seedlings." Waters M.T., Smith S.M. Mol. Plant 6:63-75(2013) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION BY LIGHT. Strain: cv. Landsberg erecta.
[7]	"Structures of D14 and D14L in the strigolactone and karrikin signaling pathways." Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T. Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), FUNCTION, MUTAGENESIS OF GLY-133.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL035601 Genomic DNA. Translation: CAB38214.1.
AL161591 Genomic DNA. Translation: CAB80412.1.
CP002687 Genomic DNA. Translation: AEE86798.1.
AY056190 mRNA. Translation: AAL07039.1.
AY091347 mRNA. Translation: AAM14286.1.

IPI

IPI00548200.

PIR

T04741.

RefSeq

NP_195463.1. NM_119911.3.

UniGene

At.66602.
At.71877.
At.72976.
At.74782.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3W06	X-ray	1.15	A	1-270	[»]
4HRX	X-ray	2.11	A	1-270	[»]
4HRY	X-ray	1.50	A	1-270	[»]
4HTA	X-ray	1.90	A	1-270	[»]
4IH1	X-ray	1.55	A	1-270	[»]

ProteinModelPortal

Q9SZU7.

SMR

Q9SZU7. Positions 6-253.

ModBase

Search...

Protein-protein interaction databases

STRING

3702.AT4G37470.1-P.

Protein family/group databases

MEROPS

S33.A29.

Proteomic databases

PaxDb

Q9SZU7.

PRIDE

Q9SZU7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT4G37470.1; AT4G37470.1; AT4G37470.

GeneID

829902.

KEGG

ath:AT4G37470.

Organism-specific databases

TAIR

At4g37470.

Phylogenomic databases

eggNOG

COG0596.

HOGENOM

HOG000251386.

InParanoid

Q9SZU7.

OMA

TNSFCRT.

PhylomeDB

Q9SZU7.

ProtClustDB

CLSN2684507.

Gene expression databases

Genevestigator

Q9SZU7.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

KAI2_ARATH

Accession

Primary (citable) accession number: Q9SZU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2013
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program