Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-transporting ATPase 10, plasma membrane-type

Gene

ACA10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the endoplasmic reticulum.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei482 – 48214-aspartylphosphate intermediateBy similarity
Metal bindingi789 – 7891MagnesiumBy similarity
Metal bindingi793 – 7931MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G29900-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 10, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 10
Gene namesi
Name:ACA10
Ordered Locus Names:At4g29900
ORF Names:F27B13.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G29900.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 180179CytoplasmicSequence analysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Topological domaini202 – 21918LumenalSequence analysisAdd
BLAST
Transmembranei220 – 24021HelicalSequence analysisAdd
BLAST
Topological domaini241 – 369129CytoplasmicSequence analysisAdd
BLAST
Transmembranei370 – 38920HelicalSequence analysisAdd
BLAST
Topological domaini390 – 42637LumenalSequence analysisAdd
BLAST
Transmembranei427 – 44418HelicalSequence analysisAdd
BLAST
Topological domaini445 – 844400CytoplasmicSequence analysisAdd
BLAST
Transmembranei845 – 86319HelicalSequence analysisAdd
BLAST
Topological domaini864 – 87411LumenalSequence analysisAdd
BLAST
Transmembranei875 – 89521HelicalSequence analysisAdd
BLAST
Topological domaini896 – 91520CytoplasmicSequence analysisAdd
BLAST
Transmembranei916 – 93823HelicalSequence analysisAdd
BLAST
Topological domaini939 – 95113LumenalSequence analysisAdd
BLAST
Transmembranei952 – 97322HelicalSequence analysisAdd
BLAST
Topological domaini974 – 99118CytoplasmicSequence analysisAdd
BLAST
Transmembranei992 – 101322HelicalSequence analysisAdd
BLAST
Topological domaini1014 – 102310LumenalSequence analysis
Transmembranei1024 – 104522HelicalSequence analysisAdd
BLAST
Topological domaini1046 – 106924CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 10691068Calcium-transporting ATPase 10, plasma membrane-typePRO_0000046416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SZR1.
PRIDEiQ9SZR1.

PTM databases

iPTMnetiQ9SZR1.
SwissPalmiQ9SZR1.

Expressioni

Gene expression databases

GenevisibleiQ9SZR1. AT.

Interactioni

Protein-protein interaction databases

BioGridi14399. 1 interaction.
STRINGi3702.AT4G29900.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SZR1.
SMRiQ9SZR1. Positions 39-92, 124-938.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 5312Interaction with calmodulinBy similarityAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9SZR1.
KOiK01537.
OMAiGGVHEIC.
PhylomeDBiQ9SZR1.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SZR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQFNNSPR GEDKDVEAGT SSFTEYEDSP FDIASTKNAP VERLRRWRQA
60 70 80 90 100
ALVLNASRRF RYTLDLKREE DKKQMLRKMR AHAQAIRAAH LFKAAASRVT
110 120 130 140 150
GIASPLPTPG GGDFGIGQEQ IVSISRDQNI GALQELGGVR GLSDLLKTNL
160 170 180 190 200
EKGIHGDDDD ILKRKSAFGS NTYPQKKGRS FWRFVWEASQ DLTLIILIVA
210 220 230 240 250
AVASLALGIK TEGIEKGWYD GISIAFAVLL VIVVTATSDY RQSLQFQNLN
260 270 280 290 300
EEKRNIRLEV TRDGRRVEIS IYDIVVGDVI PLNIGDQVPA DGVLVAGHSL
310 320 330 340 350
AVDESSMTGE SKIVQKNSTK HPFLMSGCKV ADGNGTMLVT GVGVNTEWGL
360 370 380 390 400
LMASVSEDNG GETPLQVRLN GVATFIGIVG LTVAGVVLFV LVVRYFTGHT
410 420 430 440 450
KNEQGGPQFI GGKTKFEHVL DDLVEIFTVA VTIVVVAVPE GLPLAVTLTL
460 470 480 490 500
AYSMRKMMAD KALVRRLSAC ETMGSATTIC SDKTGTLTLN EMTVVECYAG
510 520 530 540 550
LQKMDSPDSS SKLPSAFTSI LVEGIAHNTT GSVFRSESGE IQVSGSPTER
560 570 580 590 600
AILNWAIKLG MDFDALKSES SAVQFFPFNS EKKRGGVAVK SPDSSVHIHW
610 620 630 640 650
KGAAEIVLGS CTHYMDESES FVDMSEDKMG GLKDAIDDMA ARSLRCVAIA
660 670 680 690 700
FRTFEADKIP TDEEQLSRWE LPEDDLILLA IVGIKDPCRP GVKNSVLLCQ
710 720 730 740 750
QAGVKVRMVT GDNIQTAKAI ALECGILASD SDASEPNLIE GKVFRSYSEE
760 770 780 790 800
ERDRICEEIS VMGRSSPNDK LLLVQSLKRR GHVVAVTGDG TNDAPALHEA
810 820 830 840 850
DIGLAMGIQG TEVAKEKSDI IILDDNFESV VKVVRWGRSV YANIQKFIQF
860 870 880 890 900
QLTVNVAALV INVVAAISAG EVPLTAVQLL WVNLIMDTLG ALALATEPPT
910 920 930 940 950
DHLMDRAPVG RREPLITNIM WRNLFIQAMY QVTVLLILNF RGISILHLKS
960 970 980 990 1000
KPNAERVKNT VIFNAFVICQ VFNEFNARKP DEINIFRGVL RNHLFVGIIS
1010 1020 1030 1040 1050
ITIVLQVVIV EFLGTFASTT KLDWEMWLVC IGIGSISWPL AVIGKLIPVP
1060
ETPVSQYFRI NRWRRNSSG
Length:1,069
Mass (Da):116,858
Last modified:January 11, 2001 - v2
Checksum:i48CE4A4B218E2656
GO

Sequence cautioni

The sequence CAB43665.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL050352 Genomic DNA. Translation: CAB43665.1. Sequence problems.
AL161575 Genomic DNA. Translation: CAB79748.1.
CP002687 Genomic DNA. Translation: AEE85690.1.
PIRiC85349.
T08551.
RefSeqiNP_194719.1. NM_119136.3.
UniGeneiAt.31882.
At.48920.

Genome annotation databases

EnsemblPlantsiAT4G29900.1; AT4G29900.1; AT4G29900.
GeneIDi829112.
GrameneiAT4G29900.1; AT4G29900.1; AT4G29900.
KEGGiath:AT4G29900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL050352 Genomic DNA. Translation: CAB43665.1. Sequence problems.
AL161575 Genomic DNA. Translation: CAB79748.1.
CP002687 Genomic DNA. Translation: AEE85690.1.
PIRiC85349.
T08551.
RefSeqiNP_194719.1. NM_119136.3.
UniGeneiAt.31882.
At.48920.

3D structure databases

ProteinModelPortaliQ9SZR1.
SMRiQ9SZR1. Positions 39-92, 124-938.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14399. 1 interaction.
STRINGi3702.AT4G29900.1.

PTM databases

iPTMnetiQ9SZR1.
SwissPalmiQ9SZR1.

Proteomic databases

PaxDbiQ9SZR1.
PRIDEiQ9SZR1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G29900.1; AT4G29900.1; AT4G29900.
GeneIDi829112.
GrameneiAT4G29900.1; AT4G29900.1; AT4G29900.
KEGGiath:AT4G29900.

Organism-specific databases

TAIRiAT4G29900.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9SZR1.
KOiK01537.
OMAiGGVHEIC.
PhylomeDBiQ9SZR1.

Enzyme and pathway databases

BioCyciARA:AT4G29900-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9SZR1.

Gene expression databases

GenevisibleiQ9SZR1. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACA10_ARATH
AccessioniPrimary (citable) accession number: Q9SZR1
Secondary accession number(s): Q9M0D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: April 13, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.