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Protein

Serine hydroxymethyltransferase 1, mitochondrial

Gene

SHM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the photorespiratory pathway in catalyzing the interconversion of serine and glycine. Involved in controlling cell damage caused by abiotic stress, such as high light and salt and the hypersensitive defense response of plants.4 Publications

Miscellaneous

Recessive mutant shmt1-1 (shm1-3) shows enhanced susceptibility to salt stress and to biotrophic and necrotrophic pathogens.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.

Cofactori

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

  • glycine hydroxymethyltransferase activity Source: UniProtKB
  • poly(U) RNA binding Source: TAIR
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • circadian rhythm Source: UniProtKB
  • glycine biosynthetic process from serine Source: InterPro
  • glycine metabolic process Source: UniProtKB
  • L-serine metabolic process Source: UniProtKB
  • photorespiration Source: UniProtKB
  • plant-type hypersensitive response Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: UniProtKB
  • tetrahydrofolate interconversion Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT4G37930-MONOMER
MetaCyc:AT4G37930-MONOMER
ReactomeiR-ATH-196757 Metabolism of folate and pterines
R-ATH-71262 Carnitine synthesis
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase 1, mitochondrial (EC:2.1.2.1)
Short name:
AtSHMT1
Alternative name(s):
Glycine hydroxymethyltransferase 1
Serine Transhydroxymethyltransferase
Short name:
STM
Serine methylase 1
Gene namesi
Name:SHM1
Synonyms:SHMT1, STM
Ordered Locus Names:At4g37930
ORF Names:F20D10.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37930
TAIRilocus:2005518 AT4G37930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Displays a lethal photorespiratory phenotype when grown at ambient carbon dioxide.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000003256931 – 517Serine hydroxymethyltransferase 1, mitochondrialAdd BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei286N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9SZJ5
PRIDEiQ9SZJ5

PTM databases

iPTMnetiQ9SZJ5

Expressioni

Tissue specificityi

Ubiquitous. Mostly expressed in leaves, less abundant in stems, flowers and siliques, and barely detectable in roots.4 Publications

Inductioni

Circadian-regulation. Induction by light.1 Publication

Gene expression databases

ExpressionAtlasiQ9SZJ5 baseline and differential
GenevisibleiQ9SZJ5 AT

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with GLU1. Interacts with UBP16.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi15230, 6 interactors
IntActiQ9SZJ5, 2 interactors
STRINGi3702.AT4G37930.1

Structurei

3D structure databases

ProteinModelPortaliQ9SZJ5
SMRiQ9SZJ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SHMT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2467 Eukaryota
COG0112 LUCA
HOGENOMiHOG000239405
InParanoidiQ9SZJ5
KOiK00600
OMAiDWVGTYP
OrthoDBiEOG093608RQ
PhylomeDBiQ9SZJ5

Family and domain databases

CDDicd00378 SHMT, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00051 SHMT, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR001085 Ser_HO-MeTrfase
IPR019798 Ser_HO-MeTrfase_PLP_BS
PIRSFiPIRSF000412 SHMT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00096 SHMT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SZJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMAMALRRL SSSIDKPIRP LIRSTSCYMS SLPSEAVDEK ERSRVTWPKQ
60 70 80 90 100
LNAPLEEVDP EIADIIEHEK ARQWKGLELI PSENFTSVSV MQAVGSVMTN
110 120 130 140 150
KYSEGYPGAR YYGGNEYIDM AETLCQKRAL EAFRLDPEKW GVNVQPLSGS
160 170 180 190 200
PANFHVYTAL LKPHERIMAL DLPHGGHLSH GYQTDTKKIS AVSIFFETMP
210 220 230 240 250
YRLDESTGYI DYDQMEKSAT LFRPKLIVAG ASAYARLYDY ARIRKVCNKQ
260 270 280 290 300
KAVMLADMAH ISGLVAANVI PSPFDYADVV TTTTHKSLRG PRGAMIFFRK
310 320 330 340 350
GVKEINKQGK EVLYDFEDKI NQAVFPGLQG GPHNHTITGL AVALKQATTS
360 370 380 390 400
EYKAYQEQVL SNSAKFAQTL MERGYELVSG GTDNHLVLVN LKPKGIDGSR
410 420 430 440 450
VEKVLEAVHI ASNKNTVPGD VSAMVPGGIR MGTPALTSRG FVEEDFAKVA
460 470 480 490 500
EYFDKAVTIA LKVKSEAQGT KLKDFVSAME SSSTIQSEIA KLRHEVEEFA
510
KQFPTIGFEK ETMKYKN
Length:517
Mass (Da):57,401
Last modified:May 1, 2000 - v1
Checksum:i5FDB9F6085B8B19F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171D → N in AAL06913 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271726 mRNA Translation: CAB71289.1
AL035538 Genomic DNA Translation: CAB37533.1
AL161592 Genomic DNA Translation: CAB80458.1
CP002687 Genomic DNA Translation: AEE86855.1
AF428388 mRNA Translation: AAL16156.1
AY054254 mRNA Translation: AAL06913.1
AY057645 mRNA Translation: AAL15276.1
AY070726 mRNA Translation: AAL50068.1
BT006353 mRNA Translation: AAP21161.1
PIRiT05620
RefSeqiNP_195506.1, NM_119954.4
UniGeneiAt.21766

Genome annotation databases

EnsemblPlantsiAT4G37930.1; AT4G37930.1; AT4G37930
GeneIDi829949
GrameneiAT4G37930.1; AT4G37930.1; AT4G37930
KEGGiath:AT4G37930

Similar proteinsi

Entry informationi

Entry nameiGLYM1_ARATH
AccessioniPrimary (citable) accession number: Q9SZJ5
Secondary accession number(s): Q940M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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