Reviewed, UniProtKB/Swiss-Prot Q9SZC9 (AHM6_ARATH)

Names and origin

Protein names

Recommended name:
    Putative copper-transporting ATPase PAA1
    EC=3.6.3.4

Gene names

Name:	PAA1
Ordered Locus Names:	At4g33520
ORF Names:	T16L1.10, F17M5.280

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	949 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in copper transport Potential.
Catalytic activity	ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 1 HMA domain.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW photosynthetic electron transport chain Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper-exporting ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SZC9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 949	949	Putative copper-transporting ATPase PAA1		PRO_0000046403
Regions
	Topological domain	1 – 252	252	Cytoplasmic Potential
	Transmembrane	253 – 274	22	Potential
	Topological domain	275 – 286	12	Extracellular Potential
	Transmembrane	287 – 306	20	Potential
	Topological domain	307 – 313	7	Cytoplasmic Potential
	Transmembrane	314 – 334	21	Potential
	Topological domain	335 – 348	14	Extracellular Potential
	Transmembrane	349 – 369	21	Potential
	Topological domain	370 – 501	132	Cytoplasmic Potential
	Transmembrane	502 – 524	23	Potential
	Topological domain	525 – 542	18	Extracellular Potential
	Transmembrane	543 – 560	18	Potential
	Topological domain	561 – 862	302	Cytoplasmic Potential
	Transmembrane	863 – 882	20	Potential
	Topological domain	883 – 894	12	Extracellular Potential
	Transmembrane	895 – 913	19	Potential
	Topological domain	914 – 949	36	Cytoplasmic Potential
	Domain	149 – 223	75	HMA
	Compositional bias	115 – 124	10	Poly-Gly
Sites
	Active site	598	1	4-aspartylphosphate intermediate By similarity
	Metal binding	159	1	Copper Potential
	Metal binding	162	1	Copper Potential
	Metal binding	808	1	Magnesium By similarity
	Metal binding	812	1	Magnesium By similarity
Natural variations
	Natural variant	215	1	T → S in strain: cv. Landsberg erecta.
	Natural variant	238 – 239	2	KT → QP in strain: cv. Landsberg erecta.
	Natural variant	259	1	L → P in strain: cv. Landsberg erecta.
	Natural variant	384	1	P → A in strain: cv. Landsberg erecta.
	Natural variant	904	1	S → T in strain: cv. Landsberg erecta.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 716F5E6E63B7F9B8	FASTA	949	99,997
	10 20 30 40 50 60 MESTLSAFST VKATAMARSS GGPSLPLLTI SKALNRHFTG ARHLHPLLLA RCSPSVRRLG 70 80 90 100 110 120 GFHGSRFTSS NSALRSLGAA VLPVIRHRLE CLSSSSPSFR SISSGGGSGF GGYNGGSGGG 130 140 150 160 170 180 GGGGSESGDS KSKLGANASD GVSVPSSDII ILDVGGMTCG GCSASVKKIL ESQPQVASAS 190 200 210 220 230 240 VNLTTETAIV WPVPEAKSVP DWQKSLGETL ANHLTNCGFQ STPRDLVTEN FFKVFETKTK 250 260 270 280 290 300 DKQARLKESG RELAVSWALC AVCLVGHLTH FLGVNAPWIH AIHSTGFHVS LCLITLLGPG 310 320 330 340 350 360 RKLVLDGIKS LLKGSPNMNT LVGLGALSSF SVSSLAAMIP KLGWKTFFEE PVMLIAFVLL 370 380 390 400 410 420 GRNLEQRAKI KATSDMTGLL SVLPSKARLL LDGDLQNSTV EVPCNSLSVG DLVVILPGDR 430 440 450 460 470 480 VPADGVVKSG RSTIDESSFT GEPLPVTKES GSQVAAGSIN LNGTLTVEVH RSGGETAVGD 490 500 510 520 530 540 IIRLVEEAQS REAPVQQLVD KVAGRFTYGV MALSAATFTF WNLFGAHVLP SALHNGSPMS 550 560 570 580 590 600 LALQLSCSVL VVACPCALGL ATPTAMLVGT SLGARRGLLL RGGDILEKFS LVDTVVFDKT 610 620 630 640 650 660 GTLTKGHPVV TEVIIPENPR HNLNDTWSEV EVLMLAAAVE SNTTHPVGKA IVKAARARNC 670 680 690 700 710 720 QTMKAEDGTF TEEPGSGAVA IVNNKRVTVG TLEWVKRHGA TGNSLLALEE HEINNQSVVY 730 740 750 760 770 780 IGVDNTLAAV IRFEDKVRED AAQVVENLTR QGIDVYMLSG DKRNAANYVA SVVGINHERV 790 800 810 820 830 840 IAGVKPAEKK NFINELQKNK KIVAMVGDGI NDAAALASSN VGVAMGGGAG AASEVSPVVL 850 860 870 880 890 900 MGNRLTQLLD AMELSRQTMK TVKQNLWWAF GYNIVGIPIA AGVLLPLTGT MLTPSMAGAL 910 920 930 940 MGVSSLGVMT NSLLLRYRFF SNRNDKNVKP EPKEGTKQPH ENTRWKQSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding a putative metal-transporting P-type ATPase from Arabidopsis thaliana." Tabata K., Kashiwagi S., Mori H., Ueguchi C., Mizuno T. Biochim. Biophys. Acta 1326:1-6(1997) [PubMed: 9188794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89981 mRNA. Translation: BAA23769.1. AL035678 Genomic DNA. Translation: CAB38810.1. AL161583 Genomic DNA. Translation: CAB80069.1. AL031394 Genomic DNA. Translation: CAA20565.1.
IPI	IPI00541571.
PIR	T06003.
RefSeq	NP_974675.1.
UniGene	At.48932
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.A.3.5.11. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PRIDE	Q9SZC9.
Genome annotation databases
GeneID	829490.
GenomeReviews	Gene locus AT4G33520 in contig CT486007_GR.
NMPDR	fig|3702.1.peg.21396.
Organism-specific databases
TAIR	At4g33520.
Enzyme and pathway databases
BRENDA	3.6.3.4. 302.
Gene expression databases
Genevestigator	Q9SZC9.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006403. ATPase_P-typ_cat/Cu-transptr. IPR006416. ATPase_P-typ_heavy-metal. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. IPR017969. Heavy-metal-associated_CS. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 1 hit. PS50846. HMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AHM6_ARATH

Accession

Primary (citable) accession number: Q9SZC9
Secondary accession number(s): O48600, O81870

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)