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Protein

Copper-transporting ATPase PAA1, chloroplastic

Gene

PAA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates copper transfer across the plastid envelope. Required for the delivery of copper into the plastid stroma, which is essential for the function of copper proteins. Seems to be selective for monovalent copper Cu+ transport. Plays also a role in glucose signaling-mediated cell proliferation of root meristem in non-green tissues.4 Publications

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159CopperPROSITE-ProRule annotation1
Metal bindingi162CopperPROSITE-ProRule annotation1
Active sitei5984-aspartylphosphate intermediateBy similarity1
Metal bindingi808MagnesiumPROSITE-ProRule annotation1
Metal bindingi812MagnesiumPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi807 – 814ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper chaperone activity Source: TAIR
  • copper ion transmembrane transporter activity Source: TAIR
  • copper-transporting ATPase activity Source: GO_Central

GO - Biological processi

  • copper ion homeostasis Source: TAIR
  • copper ion transmembrane transport Source: UniProtKB
  • photosynthetic electron transport chain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G33520-MONOMER.
MetaCyc:MONOMER-14495.
BRENDAi3.6.3.4. 399.

Protein family/group databases

TCDBi3.A.3.5.11. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase PAA1, chloroplastic (EC:3.6.3.54)
Alternative name(s):
Protein HEAVY METAL ATPASE 6
Protein glucose insensitive root 1
Gene namesi
Name:PAA1
Synonyms:GIR1, HMA6
Ordered Locus Names:At4g33520
ORF Names:F17M5.280, T16L1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G33520.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei253 – 274HelicalSequence analysisAdd BLAST22
Transmembranei287 – 306HelicalSequence analysisAdd BLAST20
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei502 – 524HelicalSequence analysisAdd BLAST23
Transmembranei543 – 560HelicalSequence analysisAdd BLAST18
Transmembranei863 – 882HelicalSequence analysisAdd BLAST20
Transmembranei895 – 913HelicalSequence analysisAdd BLAST19

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast membrane Source: UniProtKB-SubCell
  • chloroplast stroma Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

High-chlorophyll-fluorescence phenotype. Short roots.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 103ChloroplastSequence analysisAdd BLAST103
ChainiPRO_0000046403104 – 949Copper-transporting ATPase PAA1, chloroplasticAdd BLAST846

Proteomic databases

PaxDbiQ9SZC9.
PRIDEiQ9SZC9.

Expressioni

Tissue specificityi

Expressed in the shoots and roots.1 Publication

Gene expression databases

ExpressionAtlasiQ9SZC9. baseline and differential.
GenevisibleiQ9SZC9. AT.

Interactioni

Protein-protein interaction databases

BioGridi14774. 4 interactors.
IntActiQ9SZC9. 2 interactors.
MINTiMINT-8061569.
STRINGi3702.AT4G33520.2.

Structurei

3D structure databases

ProteinModelPortaliQ9SZC9.
SMRiQ9SZC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 223HMAPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi115 – 124Poly-Gly10

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9SZC9.
OMAiHWGLATL.
PhylomeDBiQ9SZC9.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SZC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESTLSAFST VKATAMARSS GGPSLPLLTI SKALNRHFTG ARHLHPLLLA
60 70 80 90 100
RCSPSVRRLG GFHGSRFTSS NSALRSLGAA VLPVIRHRLE CLSSSSPSFR
110 120 130 140 150
SISSGGGSGF GGYNGGSGGG GGGGSESGDS KSKLGANASD GVSVPSSDII
160 170 180 190 200
ILDVGGMTCG GCSASVKKIL ESQPQVASAS VNLTTETAIV WPVPEAKSVP
210 220 230 240 250
DWQKSLGETL ANHLTNCGFQ STPRDLVTEN FFKVFETKTK DKQARLKESG
260 270 280 290 300
RELAVSWALC AVCLVGHLTH FLGVNAPWIH AIHSTGFHVS LCLITLLGPG
310 320 330 340 350
RKLVLDGIKS LLKGSPNMNT LVGLGALSSF SVSSLAAMIP KLGWKTFFEE
360 370 380 390 400
PVMLIAFVLL GRNLEQRAKI KATSDMTGLL SVLPSKARLL LDGDLQNSTV
410 420 430 440 450
EVPCNSLSVG DLVVILPGDR VPADGVVKSG RSTIDESSFT GEPLPVTKES
460 470 480 490 500
GSQVAAGSIN LNGTLTVEVH RSGGETAVGD IIRLVEEAQS REAPVQQLVD
510 520 530 540 550
KVAGRFTYGV MALSAATFTF WNLFGAHVLP SALHNGSPMS LALQLSCSVL
560 570 580 590 600
VVACPCALGL ATPTAMLVGT SLGARRGLLL RGGDILEKFS LVDTVVFDKT
610 620 630 640 650
GTLTKGHPVV TEVIIPENPR HNLNDTWSEV EVLMLAAAVE SNTTHPVGKA
660 670 680 690 700
IVKAARARNC QTMKAEDGTF TEEPGSGAVA IVNNKRVTVG TLEWVKRHGA
710 720 730 740 750
TGNSLLALEE HEINNQSVVY IGVDNTLAAV IRFEDKVRED AAQVVENLTR
760 770 780 790 800
QGIDVYMLSG DKRNAANYVA SVVGINHERV IAGVKPAEKK NFINELQKNK
810 820 830 840 850
KIVAMVGDGI NDAAALASSN VGVAMGGGAG AASEVSPVVL MGNRLTQLLD
860 870 880 890 900
AMELSRQTMK TVKQNLWWAF GYNIVGIPIA AGVLLPLTGT MLTPSMAGAL
910 920 930 940
MGVSSLGVMT NSLLLRYRFF SNRNDKNVKP EPKEGTKQPH ENTRWKQSS
Length:949
Mass (Da):99,997
Last modified:May 1, 2000 - v1
Checksum:i716F5E6E63B7F9B8
GO
Isoform 2 (identifier: Q9SZC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-237: DLVTENFFKVFET → GEVPEDIAGEFAP
     238-949: Missing.

Show »
Length:237
Mass (Da):24,013
Checksum:iC06AAE221D146D34
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti215T → S in strain: cv. Landsberg erecta. 1
Natural varianti238 – 239KT → QP in strain: cv. Landsberg erecta. 2
Natural varianti259L → P in strain: cv. Landsberg erecta. 1
Natural varianti384P → A in strain: cv. Landsberg erecta. 1
Natural varianti904S → T in strain: cv. Landsberg erecta. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040517225 – 237DLVTE…KVFET → GEVPEDIAGEFAP in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_040518238 – 949Missing in isoform 2. 1 PublicationAdd BLAST712

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89981 mRNA. Translation: BAA23769.1.
AL031394 Genomic DNA. Translation: CAA20565.1.
AL035678 Genomic DNA. Translation: CAB38810.1.
AL161583 Genomic DNA. Translation: CAB80069.1.
CP002687 Genomic DNA. Translation: AEE86239.1.
CP002687 Genomic DNA. Translation: AEE86240.1.
AY059869 mRNA. Translation: AAL24351.1.
AY093320 mRNA. Translation: AAM13319.1.
AK229051 mRNA. Translation: BAF00934.1.
PIRiT06003.
RefSeqiNP_567924.1. NM_119506.4. [Q9SZC9-2]
NP_974675.1. NM_202946.3. [Q9SZC9-1]
UniGeneiAt.48932.

Genome annotation databases

EnsemblPlantsiAT4G33520.2; AT4G33520.2; AT4G33520. [Q9SZC9-1]
GeneIDi829490.
GrameneiAT4G33520.2; AT4G33520.2; AT4G33520.
KEGGiath:AT4G33520.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89981 mRNA. Translation: BAA23769.1.
AL031394 Genomic DNA. Translation: CAA20565.1.
AL035678 Genomic DNA. Translation: CAB38810.1.
AL161583 Genomic DNA. Translation: CAB80069.1.
CP002687 Genomic DNA. Translation: AEE86239.1.
CP002687 Genomic DNA. Translation: AEE86240.1.
AY059869 mRNA. Translation: AAL24351.1.
AY093320 mRNA. Translation: AAM13319.1.
AK229051 mRNA. Translation: BAF00934.1.
PIRiT06003.
RefSeqiNP_567924.1. NM_119506.4. [Q9SZC9-2]
NP_974675.1. NM_202946.3. [Q9SZC9-1]
UniGeneiAt.48932.

3D structure databases

ProteinModelPortaliQ9SZC9.
SMRiQ9SZC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14774. 4 interactors.
IntActiQ9SZC9. 2 interactors.
MINTiMINT-8061569.
STRINGi3702.AT4G33520.2.

Protein family/group databases

TCDBi3.A.3.5.11. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiQ9SZC9.
PRIDEiQ9SZC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G33520.2; AT4G33520.2; AT4G33520. [Q9SZC9-1]
GeneIDi829490.
GrameneiAT4G33520.2; AT4G33520.2; AT4G33520.
KEGGiath:AT4G33520.

Organism-specific databases

TAIRiAT4G33520.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9SZC9.
OMAiHWGLATL.
PhylomeDBiQ9SZC9.

Enzyme and pathway databases

BioCyciARA:AT4G33520-MONOMER.
MetaCyc:MONOMER-14495.
BRENDAi3.6.3.4. 399.

Miscellaneous databases

PROiQ9SZC9.

Gene expression databases

ExpressionAtlasiQ9SZC9. baseline and differential.
GenevisibleiQ9SZC9. AT.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMA6_ARATH
AccessioniPrimary (citable) accession number: Q9SZC9
Secondary accession number(s): O48600
, O81870, Q0WPL5, Q93YP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.