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Protein

Photosystem I chlorophyll a/b-binding protein 2, chloroplastic

Gene

LHCA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated, here photosystem I.1 Publication

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.4 Publications

Absorptioni

Abs(max)=~695 nm

Emission maxima at 702 nm.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity
Binding sitei75 – 751Chlorophyll-a 1; via amide nitrogenBy similarity
Binding sitei81 – 811Chlorophyll-a 1By similarity
Metal bindingi94 – 941Magnesium (chlorophyll-a 1 axial ligand)By similarity
Binding sitei99 – 991Chlorophyll-b 2By similarity
Metal bindingi152 – 1521Magnesium (chlorophyll-b 3 axial ligand)By similarity
Binding sitei155 – 1551Chlorophyll-b 4By similarity
Binding sitei208 – 2081Chlorophyll-a 5By similarity
Metal bindingi209 – 2091Magnesium (chlorophyll-a 3 axial ligand)By similarity
Metal bindingi212 – 2121Magnesium (chlorophyll-a 4 axial ligand)By similarity
Binding sitei214 – 2141Chlorophyll-a 1By similarity
Metal bindingi226 – 2261Magnesium (chlorophyll-a 5 axial ligand)By similarity
Metal bindingi241 – 2411Magnesium (chlorophyll-a 6 axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • photosynthesis, light harvesting in photosystem I Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • response to cold Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to light stimulus Source: GO_Central
  • response to low light intensity stimulus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Protein family/group databases

TCDBi5.B.4.1.1. the plant photosystem i supercomplex (psi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem I chlorophyll a/b-binding protein 2, chloroplastic1 Publication
Short name:
Lhca21 Publication
Alternative name(s):
LHCI type III LHCA2Curated
Gene namesi
Name:LHCA21 Publication
Ordered Locus Names:At3g61470Imported
ORF Names:F2A19.70Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G61470.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei100 – 12021HelicalSequence analysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence analysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: GO_Central
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • photosystem I Source: UniProtKB-KW
  • plastoglobule Source: GO_Central
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Depletion of LHCA3 levels (at protein level).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343ChloroplastBy similarityAdd
BLAST
Chaini44 – 257214Photosystem I chlorophyll a/b-binding protein 2, chloroplasticPRO_0000435447Add
BLAST

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.By similarity

Proteomic databases

PaxDbiQ9SYW8.

Expressioni

Inductioni

Induced by low light (LL) but repressed by high light (HL). Inhibited by cold.1 Publication

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins (PubMed:19139095). Red-emitting heterodimers with LHCA3 and LHCA5 (PubMed:17107674, PubMed:21083539, PubMed:15356385). Binds to carotenoids (PubMed:17326666).5 Publications

Protein-protein interaction databases

STRINGi3702.AT3G61470.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SYW8.
SMRiQ9SYW8. Positions 50-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.Curated

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IID9. Eukaryota.
ENOG410XTCT. LUCA.
HOGENOMiHOG000238033.
KOiK08908.
OMAiRIATSPQ.
PhylomeDBiQ9SYW8.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SYW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLCASSA IAAISSPSFL GGKKLRLKKK LTVPAVSRPD ASVRAVAADP
60 70 80 90 100
DRPIWFPGST PPEWLDGSLP GDFGFDPLGL SSDPDSLKWN VQAEIVHCRW
110 120 130 140 150
AMLGAAGIFI PEFLTKIGIL NTPSWYTAGE QEYFTDKTTL FVVELILIGW
160 170 180 190 200
AEGRRWADII KPGSVNTDPV FPNNKLTGTD VGYPGGLWFD PLGWGSGSPA
210 220 230 240 250
KLKELRTKEI KNGRLAMLAV MGAWFQHIYT GTGPIDNLFA HLADPGHATI

FAAFTPK
Length:257
Mass (Da):27,755
Last modified:May 1, 2000 - v1
Checksum:i208A86988298428E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391P → Q in CAB71077 (PubMed:11130713).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134120 mRNA. Translation: AAD28767.1.
AL132962 Genomic DNA. Translation: CAB71077.1.
CP002686 Genomic DNA. Translation: AEE80209.1.
AY054670 mRNA. Translation: AAK96861.1.
AY065429 mRNA. Translation: AAL38870.1.
AY072483 mRNA. Translation: AAL66898.1.
BT002070 mRNA. Translation: AAN72081.1.
PIRiT47939.
T50550.
RefSeqiNP_191706.2. NM_116012.4.
UniGeneiAt.46743.
At.55066.

Genome annotation databases

EnsemblPlantsiAT3G61470.1; AT3G61470.1; AT3G61470.
GeneIDi825320.
GrameneiAT3G61470.1; AT3G61470.1; AT3G61470.
KEGGiath:AT3G61470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134120 mRNA. Translation: AAD28767.1.
AL132962 Genomic DNA. Translation: CAB71077.1.
CP002686 Genomic DNA. Translation: AEE80209.1.
AY054670 mRNA. Translation: AAK96861.1.
AY065429 mRNA. Translation: AAL38870.1.
AY072483 mRNA. Translation: AAL66898.1.
BT002070 mRNA. Translation: AAN72081.1.
PIRiT47939.
T50550.
RefSeqiNP_191706.2. NM_116012.4.
UniGeneiAt.46743.
At.55066.

3D structure databases

ProteinModelPortaliQ9SYW8.
SMRiQ9SYW8. Positions 50-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G61470.1.

Protein family/group databases

TCDBi5.B.4.1.1. the plant photosystem i supercomplex (psi) family.

Proteomic databases

PaxDbiQ9SYW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G61470.1; AT3G61470.1; AT3G61470.
GeneIDi825320.
GrameneiAT3G61470.1; AT3G61470.1; AT3G61470.
KEGGiath:AT3G61470.

Organism-specific databases

TAIRiAT3G61470.

Phylogenomic databases

eggNOGiENOG410IID9. Eukaryota.
ENOG410XTCT. LUCA.
HOGENOMiHOG000238033.
KOiK08908.
OMAiRIATSPQ.
PhylomeDBiQ9SYW8.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A guide to the Lhc genes and their relatives in Arabidopsis."
    Jansson S.
    Trends Plant Sci. 4:236-240(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The PSI-K subunit of photosystem I is involved in the interaction between light-harvesting complex I and the photosystem I reaction center core."
    Jensen P.E., Gilpin M., Knoetzel J., Scheller H.V.
    J. Biol. Chem. 275:24701-24708(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  6. "The properties of the chlorophyll a/b-binding proteins Lhca2 and Lhca3 studied in vivo using antisense inhibition."
    Ganeteg U., Strand A., Gustafsson P., Jansson S.
    Plant Physiol. 127:150-158(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  7. "Pigment organization and energy transfer dynamics in isolated photosystem I (PSI) complexes from Arabidopsis thaliana depleted of the PSI-G, PSI-K, PSI-L, or PSI-N subunit."
    Ihalainen J.A., Jensen P.E., Haldrup A., van Stokkum I.H.M., van Grondelle R., Scheller H.V., Dekker J.P.
    Biophys. J. 83:2190-2201(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON PHOTOSYSTEM I ANTENNA.
  8. "Lhca5--an LHC-type protein associated with photosystem I."
    Ganeteg U., Klimmek F., Jansson S.
    Plant Mol. Biol. 54:641-651(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LHCA5, INDUCTION BY LIGHT AND COLD.
    Strain: cv. C24 and cv. Columbia.
  9. "Pigment binding, fluorescence properties, and oligomerization behavior of Lhca5, a novel light-harvesting protein."
    Storf S., Jansson S., Schmid V.H.R.
    J. Biol. Chem. 280:5163-5168(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR.
  10. Cited for: INTERACTION WITH LHCA3 AND LHCA5.
    Strain: cv. Columbia.
  11. "Singlet and triplet state transitions of carotenoids in the antenna complexes of higher-plant photosystem I."
    Croce R., Mozzo M., Morosinotto T., Romeo A., Hienerwadel R., Bassi R.
    Biochemistry 46:3846-3855(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CAROTENOIDS, COFACTOR.
  12. "The role of Lhca complexes in the supramolecular organization of higher plant photosystem I."
    Wientjes E., Oostergetel G.T., Jansson S., Boekema E.J., Croce R.
    J. Biol. Chem. 284:7803-7810(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, SUBUNIT.
  13. "The light-harvesting complexes of higher-plant Photosystem I: Lhca1/4 and Lhca2/3 form two red-emitting heterodimers."
    Wientjes E., Croce R.
    Biochem. J. 433:477-485(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, COFACTOR.
    Strain: cv. Columbia.
  14. "The role of the individual Lhcas in photosystem I excitation energy trapping."
    Wientjes E., van Stokkum I.H.M., van Amerongen H., Croce R.
    Biophys. J. 101:745-754(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiLHCA2_ARATH
AccessioniPrimary (citable) accession number: Q9SYW8
Secondary accession number(s): Q9M320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: May 1, 2000
Last modified: April 13, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.