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Q9SYM4

- TPS1_ARATH

UniProt

Q9SYM4 - TPS1_ARATH

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1

Gene

TPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Required for normal embryo development, vegetative growth and transition to flowering. Regulates embryo growth, cell wall deposition, starch and sucrose degradation, but not cell differentiation. Involved in the regulation of glucose sensing and signaling genes during plant development.9 Publications

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: TAIR

    GO - Biological processi

    1. cell division Source: TAIR
    2. embryo development ending in seed dormancy Source: TAIR
    3. plant-type cell wall biogenesis Source: TAIR
    4. sugar mediated signaling pathway Source: TAIR
    5. trehalose biosynthetic process Source: InterPro
    6. trehalose metabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 (EC:2.4.1.15)
    Alternative name(s):
    Trehalose-6-phosphate synthase 1
    Short name:
    AtTPS1
    Gene namesi
    Name:TPS1
    Ordered Locus Names:At1g78580
    ORF Names:T30F21.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G78580.

    Subcellular locationi

    Vacuole 1 Publication. Secretedcell wall 1 Publication. Cytoplasm 1 Publication
    Note: Determined in transgenic tobacco plants expressing TPS1.

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell wall, Cytoplasm, Secreted, Vacuole

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethality.4 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi17 – 171R → Q: 3-fold increase in activity. 1 Publication
    Mutagenesisi27 – 271L → P: 4-fold increase in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1PRO_0000324822Add
    BLAST

    Proteomic databases

    PaxDbiQ9SYM4.
    PRIDEiQ9SYM4.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, leaves, roots, stems, flowers and siliques.3 Publications

    Developmental stagei

    Up-regulated during seed development.1 Publication

    Inductioni

    Low induction by sucrose after 24 hours. Down-regulated by dark incubation.2 Publications

    Gene expression databases

    GenevestigatoriQ9SYM4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SYM4.
    SMRiQ9SYM4. Positions 93-561.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni92 – 559468GlycosyltransferaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi71 – 766Poly-Ala
    Compositional biasi822 – 89069Ser-richAdd
    BLAST

    Domaini

    The N-terminal part (1-88) has an inhibitory function on the enzymatic activity.

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
    In the C-terminal section; belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191477.
    InParanoidiQ9SYM4.
    KOiK16055.
    OMAiWHYRTAD.
    PhylomeDBiQ9SYM4.

    Family and domain databases

    Gene3Di3.40.50.1000. 3 hits.
    InterProiIPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR012766. Trehalose_OtsA.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9SYM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGNKYNCSS SHIPLSRTER LLRDRELREK RKSNRARNPN DVAGSSENSE    50
    NDLRLEGDSS RQYVEQYLEG AAAAMAHDDA CERQEVRPYN RQRLLVVANR 100
    LPVSAVRRGE DSWSLEISAG GLVSALLGVK EFEARWIGWA GVNVPDEVGQ 150
    KALSKALAEK RCIPVFLDEE IVHQYYNGYC NNILWPLFHY LGLPQEDRLA 200
    TTRSFQSQFA AYKKANQMFA DVVNEHYEEG DVVWCHDYHL MFLPKCLKEY 250
    NSKMKVGWFL HTPFPSSEIH RTLPSRSELL RSVLAADLVG FHTYDYARHF 300
    VSACTRILGL EGTPEGVEDQ GRLTRVAAFP IGIDSDRFIR ALEVPEVIQH 350
    MKELKERFAG RKVMLGVDRL DMIKGIPQKI LAFEKFLEEN ANWRDKVVLL 400
    QIAVPTRTDV PEYQKLTSQV HEIVGRINGR FGTLTAVPIH HLDRSLDFHA 450
    LCALYAVTDV ALVTSLRDGM NLVSYEFVAC QEAKKGVLIL SEFAGAAQSL 500
    GAGAILVNPW NITEVAASIG QALNMTAEER EKRHRHNFHH VKTHTAQEWA 550
    ETFVSELNDT VIEAQLRISK VPPELPQHDA IQRYSKSNNR LLILGFNATL 600
    TEPVDNQGRR GDQIKEMDLN LHPELKGPLK ALCSDPSTTI VVLSGSSRSV 650
    LDKNFGEYDM WLAAENGMFL RLTNGEWMTT MPEHLNMEWV DSVKHVFKYF 700
    TERTPRSHFE TRDTSLIWNY KYADIEFGRL QARDLLQHLW TGPISNASVD 750
    VVQGSRSVEV RAVGVTKGAA IDRILGEIVH SKSMTTPIDY VLCIGHFLGK 800
    DEDVYTFFEP ELPSDMPAIA RSRPSSDSGA KSSSGDRRPP SKSTHNNNKS 850
    GSKSSSSSNS NNNNKSSQRS LQSERKSGSN HSLGNSRRPS PEKISWNVLD 900
    LKGENYFSCA VGRTRTNARY LLGSPDDVVC FLEKLADTTS SP 942
    Length:942
    Mass (Da):105,976
    Last modified:May 1, 2000 - v1
    Checksum:iB22AC11EA34CDDDB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051A → P in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti348 – 3481I → K in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti359 – 3602AG → TD in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti401 – 4011Q → K in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti408 – 4081T → P in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti415 – 4151K → T in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti428 – 4281N → I in CAA69879. (PubMed:9681010)Curated
    Sequence conflicti431 – 4311F → L in CAA69879. (PubMed:9681010)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08568 mRNA. Translation: CAA69879.1.
    AC007260 Genomic DNA. Translation: AAD30578.1.
    CP002684 Genomic DNA. Translation: AEE36123.1.
    AF370287 mRNA. Translation: AAK44102.2.
    PIRiD96814.
    RefSeqiNP_177979.1. NM_106505.4.
    UniGeneiAt.198.
    At.71030.

    Genome annotation databases

    EnsemblPlantsiAT1G78580.1; AT1G78580.1; AT1G78580.
    GeneIDi844194.
    KEGGiath:AT1G78580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08568 mRNA. Translation: CAA69879.1 .
    AC007260 Genomic DNA. Translation: AAD30578.1 .
    CP002684 Genomic DNA. Translation: AEE36123.1 .
    AF370287 mRNA. Translation: AAK44102.2 .
    PIRi D96814.
    RefSeqi NP_177979.1. NM_106505.4.
    UniGenei At.198.
    At.71030.

    3D structure databases

    ProteinModelPortali Q9SYM4.
    SMRi Q9SYM4. Positions 93-561.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Proteomic databases

    PaxDbi Q9SYM4.
    PRIDEi Q9SYM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G78580.1 ; AT1G78580.1 ; AT1G78580 .
    GeneIDi 844194.
    KEGGi ath:AT1G78580.

    Organism-specific databases

    TAIRi AT1G78580.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191477.
    InParanoidi Q9SYM4.
    KOi K16055.
    OMAi WHYRTAD.
    PhylomeDBi Q9SYM4.

    Gene expression databases

    Genevestigatori Q9SYM4.

    Family and domain databases

    Gene3Di 3.40.50.1000. 3 hits.
    InterProi IPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR012766. Trehalose_OtsA.
    IPR003337. Trehalose_PPase.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase."
      Blazquez M.A., Santos E., Flores C.L., Martinez-Zapater J.M., Salinas J., Gancedo C.
      Plant J. 13:685-689(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 389-942.
      Strain: cv. Columbia.
    5. "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
      Leyman B., Van Dijck P., Thevelein J.M.
      Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues."
      Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A.
      J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Landsberg erecta.
    7. "Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeast."
      Van Dijck P., Mascorro-Gallardo J.O., De Bus M., Royackers K., Iturriaga G., Thevelein J.M.
      Biochem. J. 366:63-71(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ARG-17 AND LEU-27.
    8. "Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation."
      Eastmond P.J., van Dijken A.J.H., Spielman M., Kerr A., Tissier A.F., Dickinson H.G., Jones J.D.G., Smeekens S.C., Graham I.A.
      Plant J. 29:225-235(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    9. "Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana."
      Schluepmann H., Pellny T., van Dijken A.J.H., Smeekens S.C.M., Paul M.
      Proc. Natl. Acad. Sci. U.S.A. 100:6849-6854(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation."
      Schluepmann H., van Dijken A.J.H., Aghdasi M., Wobbes B., Paul M., Smeekens S.C.M.
      Plant Physiol. 135:879-890(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY SUCROSE.
    11. "Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering."
      van Dijken A.J.H., Schluepmann H., Smeekens S.C.M.
      Plant Physiol. 135:969-977(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    12. Cited for: FUNCTION.
    13. "The role of trehalose-6-phosphate synthase in Arabidopsis embryo development."
      Gomez L.D., Baud S., Graham I.A.
      Biochem. Soc. Trans. 33:280-282(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    14. "Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulation."
      Gomez L.D., Baud S., Gilday A., Li Y., Graham I.A.
      Plant J. 46:69-84(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    15. "Immunogold localization of trehalose-6-phosphate synthase in leaf segments of wild-type and transgenic tobacco plants expressing the AtTPS1 gene from Arabidopsis thaliana."
      Almeida A.M., Santos M., Villalobos E., Araujo S.S., van Dijck P., Leyman B., Cardoso L.A., Santos D., Fevereiro P.S., Torne J.M.
      Protoplasma 230:41-49(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTPS1_ARATH
    AccessioniPrimary (citable) accession number: Q9SYM4
    Secondary accession number(s): P93653, Q94K55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3