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Q9SYM4

- TPS1_ARATH

UniProt

Q9SYM4 - TPS1_ARATH

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Protein
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1
Gene
TPS1, At1g78580, T30F21.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for normal embryo development, vegetative growth and transition to flowering. Regulates embryo growth, cell wall deposition, starch and sucrose degradation, but not cell differentiation. Involved in the regulation of glucose sensing and signaling genes during plant development.9 Publications

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: TAIR

GO - Biological processi

  1. cell division Source: TAIR
  2. embryo development ending in seed dormancy Source: TAIR
  3. plant-type cell wall biogenesis Source: TAIR
  4. sugar mediated signaling pathway Source: TAIR
  5. trehalose biosynthetic process Source: InterPro
  6. trehalose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase 1
Short name:
AtTPS1
Gene namesi
Name:TPS1
Ordered Locus Names:At1g78580
ORF Names:T30F21.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G78580.

Subcellular locationi

Vacuole. Secretedcell wall. Cytoplasm
Note: Determined in transgenic tobacco plants expressing TPS1.1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
  3. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted, Vacuole

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi17 – 171R → Q: 3-fold increase in activity. 1 Publication
Mutagenesisi27 – 271L → P: 4-fold increase in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1
PRO_0000324822Add
BLAST

Proteomic databases

PaxDbiQ9SYM4.
PRIDEiQ9SYM4.

Expressioni

Tissue specificityi

Expressed in seedlings, leaves, roots, stems, flowers and siliques.3 Publications

Developmental stagei

Up-regulated during seed development.1 Publication

Inductioni

Low induction by sucrose after 24 hours. Down-regulated by dark incubation.2 Publications

Gene expression databases

GenevestigatoriQ9SYM4.

Structurei

3D structure databases

ProteinModelPortaliQ9SYM4.
SMRiQ9SYM4. Positions 93-561.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni92 – 559468Glycosyltransferase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 766Poly-Ala
Compositional biasi822 – 89069Ser-rich
Add
BLAST

Domaini

The N-terminal part (1-88) has an inhibitory function on the enzymatic activity.

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.
In the C-terminal section; belongs to the trehalose phosphatase family.

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191477.
InParanoidiQ9SYM4.
KOiK16055.
OMAiWHYRTAD.
PhylomeDBiQ9SYM4.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR012766. Trehalose_OtsA.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SYM4-1 [UniParc]FASTAAdd to Basket

« Hide

MPGNKYNCSS SHIPLSRTER LLRDRELREK RKSNRARNPN DVAGSSENSE    50
NDLRLEGDSS RQYVEQYLEG AAAAMAHDDA CERQEVRPYN RQRLLVVANR 100
LPVSAVRRGE DSWSLEISAG GLVSALLGVK EFEARWIGWA GVNVPDEVGQ 150
KALSKALAEK RCIPVFLDEE IVHQYYNGYC NNILWPLFHY LGLPQEDRLA 200
TTRSFQSQFA AYKKANQMFA DVVNEHYEEG DVVWCHDYHL MFLPKCLKEY 250
NSKMKVGWFL HTPFPSSEIH RTLPSRSELL RSVLAADLVG FHTYDYARHF 300
VSACTRILGL EGTPEGVEDQ GRLTRVAAFP IGIDSDRFIR ALEVPEVIQH 350
MKELKERFAG RKVMLGVDRL DMIKGIPQKI LAFEKFLEEN ANWRDKVVLL 400
QIAVPTRTDV PEYQKLTSQV HEIVGRINGR FGTLTAVPIH HLDRSLDFHA 450
LCALYAVTDV ALVTSLRDGM NLVSYEFVAC QEAKKGVLIL SEFAGAAQSL 500
GAGAILVNPW NITEVAASIG QALNMTAEER EKRHRHNFHH VKTHTAQEWA 550
ETFVSELNDT VIEAQLRISK VPPELPQHDA IQRYSKSNNR LLILGFNATL 600
TEPVDNQGRR GDQIKEMDLN LHPELKGPLK ALCSDPSTTI VVLSGSSRSV 650
LDKNFGEYDM WLAAENGMFL RLTNGEWMTT MPEHLNMEWV DSVKHVFKYF 700
TERTPRSHFE TRDTSLIWNY KYADIEFGRL QARDLLQHLW TGPISNASVD 750
VVQGSRSVEV RAVGVTKGAA IDRILGEIVH SKSMTTPIDY VLCIGHFLGK 800
DEDVYTFFEP ELPSDMPAIA RSRPSSDSGA KSSSGDRRPP SKSTHNNNKS 850
GSKSSSSSNS NNNNKSSQRS LQSERKSGSN HSLGNSRRPS PEKISWNVLD 900
LKGENYFSCA VGRTRTNARY LLGSPDDVVC FLEKLADTTS SP 942
Length:942
Mass (Da):105,976
Last modified:May 1, 2000 - v1
Checksum:iB22AC11EA34CDDDB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051A → P in CAA69879. 1 Publication
Sequence conflicti348 – 3481I → K in CAA69879. 1 Publication
Sequence conflicti359 – 3602AG → TD in CAA69879. 1 Publication
Sequence conflicti401 – 4011Q → K in CAA69879. 1 Publication
Sequence conflicti408 – 4081T → P in CAA69879. 1 Publication
Sequence conflicti415 – 4151K → T in CAA69879. 1 Publication
Sequence conflicti428 – 4281N → I in CAA69879. 1 Publication
Sequence conflicti431 – 4311F → L in CAA69879. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y08568 mRNA. Translation: CAA69879.1.
AC007260 Genomic DNA. Translation: AAD30578.1.
CP002684 Genomic DNA. Translation: AEE36123.1.
AF370287 mRNA. Translation: AAK44102.2.
PIRiD96814.
RefSeqiNP_177979.1. NM_106505.4.
UniGeneiAt.198.
At.71030.

Genome annotation databases

EnsemblPlantsiAT1G78580.1; AT1G78580.1; AT1G78580.
GeneIDi844194.
KEGGiath:AT1G78580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y08568 mRNA. Translation: CAA69879.1 .
AC007260 Genomic DNA. Translation: AAD30578.1 .
CP002684 Genomic DNA. Translation: AEE36123.1 .
AF370287 mRNA. Translation: AAK44102.2 .
PIRi D96814.
RefSeqi NP_177979.1. NM_106505.4.
UniGenei At.198.
At.71030.

3D structure databases

ProteinModelPortali Q9SYM4.
SMRi Q9SYM4. Positions 93-561.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbi Q9SYM4.
PRIDEi Q9SYM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G78580.1 ; AT1G78580.1 ; AT1G78580 .
GeneIDi 844194.
KEGGi ath:AT1G78580.

Organism-specific databases

TAIRi AT1G78580.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191477.
InParanoidi Q9SYM4.
KOi K16055.
OMAi WHYRTAD.
PhylomeDBi Q9SYM4.

Gene expression databases

Genevestigatori Q9SYM4.

Family and domain databases

Gene3Di 3.40.50.1000. 3 hits.
InterProi IPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR012766. Trehalose_OtsA.
IPR003337. Trehalose_PPase.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase."
    Blazquez M.A., Santos E., Flores C.L., Martinez-Zapater J.M., Salinas J., Gancedo C.
    Plant J. 13:685-689(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 389-942.
    Strain: cv. Columbia.
  5. "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
    Leyman B., Van Dijck P., Thevelein J.M.
    Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues."
    Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A.
    J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Landsberg erecta.
  7. "Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeast."
    Van Dijck P., Mascorro-Gallardo J.O., De Bus M., Royackers K., Iturriaga G., Thevelein J.M.
    Biochem. J. 366:63-71(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-17 AND LEU-27.
  8. "Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation."
    Eastmond P.J., van Dijken A.J.H., Spielman M., Kerr A., Tissier A.F., Dickinson H.G., Jones J.D.G., Smeekens S.C., Graham I.A.
    Plant J. 29:225-235(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
  9. "Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana."
    Schluepmann H., Pellny T., van Dijken A.J.H., Smeekens S.C.M., Paul M.
    Proc. Natl. Acad. Sci. U.S.A. 100:6849-6854(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation."
    Schluepmann H., van Dijken A.J.H., Aghdasi M., Wobbes B., Paul M., Smeekens S.C.M.
    Plant Physiol. 135:879-890(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SUCROSE.
  11. "Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering."
    van Dijken A.J.H., Schluepmann H., Smeekens S.C.M.
    Plant Physiol. 135:969-977(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. Cited for: FUNCTION.
  13. "The role of trehalose-6-phosphate synthase in Arabidopsis embryo development."
    Gomez L.D., Baud S., Graham I.A.
    Biochem. Soc. Trans. 33:280-282(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  14. "Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulation."
    Gomez L.D., Baud S., Gilday A., Li Y., Graham I.A.
    Plant J. 46:69-84(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  15. "Immunogold localization of trehalose-6-phosphate synthase in leaf segments of wild-type and transgenic tobacco plants expressing the AtTPS1 gene from Arabidopsis thaliana."
    Almeida A.M., Santos M., Villalobos E., Araujo S.S., van Dijck P., Leyman B., Cardoso L.A., Santos D., Fevereiro P.S., Torne J.M.
    Protoplasma 230:41-49(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTPS1_ARATH
AccessioniPrimary (citable) accession number: Q9SYM4
Secondary accession number(s): P93653, Q94K55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi