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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1

Gene

TPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal embryo development, vegetative growth and transition to flowering. Regulates embryo growth, cell wall deposition, starch and sucrose degradation, but not cell differentiation. Involved in the regulation of glucose sensing and signaling genes during plant development.9 Publications

Catalytic activityi

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

  • alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: TAIR
  • phosphatase activity Source: GO_Central

GO - Biological processi

  • cell division Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • plant-type cell wall biogenesis Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • trehalose biosynthetic process Source: GO_Central
  • trehalose metabolic process Source: TAIR
  • trehalose metabolism in response to stress Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.15 399

Protein family/group databases

CAZyiGT20 Glycosyltransferase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase 1
Short name:
AtTPS1
Gene namesi
Name:TPS1
Ordered Locus Names:At1g78580
ORF Names:T30F21.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G78580
TAIRilocus:2202990 AT1G78580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted, Vacuole

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17R → Q: 3-fold increase in activity. 1 Publication1
Mutagenesisi27L → P: 4-fold increase in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003248221 – 942Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1Add BLAST942

Proteomic databases

PaxDbiQ9SYM4
PRIDEiQ9SYM4

PTM databases

iPTMnetiQ9SYM4

Expressioni

Tissue specificityi

Expressed in seedlings, leaves, roots, stems, flowers and siliques.3 Publications

Developmental stagei

Up-regulated during seed development.1 Publication

Inductioni

Low induction by sucrose after 24 hours. Down-regulated by dark incubation.2 Publications

Gene expression databases

ExpressionAtlasiQ9SYM4 baseline and differential
GenevisibleiQ9SYM4 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G78580.1

Structurei

3D structure databases

ProteinModelPortaliQ9SYM4
SMRiQ9SYM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 559GlycosyltransferaseAdd BLAST468

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi71 – 76Poly-Ala6
Compositional biasi822 – 890Ser-richAdd BLAST69

Domaini

The N-terminal part (1-88) has an inhibitory function on the enzymatic activity.

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the trehalose phosphatase family.Curated

Phylogenomic databases

eggNOGiKOG1050 Eukaryota
COG0380 LUCA
COG1877 LUCA
HOGENOMiHOG000191477
InParanoidiQ9SYM4
KOiK16055
OMAiKGTDAKW
OrthoDBiEOG093602LI
PhylomeDBiQ9SYM4

Family and domain databases

CDDicd03788 GT1_TPS, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR001830 Glyco_trans_20
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR012766 Trehalose_OtsA
IPR003337 Trehalose_PPase
PfamiView protein in Pfam
PF00982 Glyco_transf_20, 1 hit
PF02358 Trehalose_PPase, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02400 trehalose_OtsA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SYM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGNKYNCSS SHIPLSRTER LLRDRELREK RKSNRARNPN DVAGSSENSE
60 70 80 90 100
NDLRLEGDSS RQYVEQYLEG AAAAMAHDDA CERQEVRPYN RQRLLVVANR
110 120 130 140 150
LPVSAVRRGE DSWSLEISAG GLVSALLGVK EFEARWIGWA GVNVPDEVGQ
160 170 180 190 200
KALSKALAEK RCIPVFLDEE IVHQYYNGYC NNILWPLFHY LGLPQEDRLA
210 220 230 240 250
TTRSFQSQFA AYKKANQMFA DVVNEHYEEG DVVWCHDYHL MFLPKCLKEY
260 270 280 290 300
NSKMKVGWFL HTPFPSSEIH RTLPSRSELL RSVLAADLVG FHTYDYARHF
310 320 330 340 350
VSACTRILGL EGTPEGVEDQ GRLTRVAAFP IGIDSDRFIR ALEVPEVIQH
360 370 380 390 400
MKELKERFAG RKVMLGVDRL DMIKGIPQKI LAFEKFLEEN ANWRDKVVLL
410 420 430 440 450
QIAVPTRTDV PEYQKLTSQV HEIVGRINGR FGTLTAVPIH HLDRSLDFHA
460 470 480 490 500
LCALYAVTDV ALVTSLRDGM NLVSYEFVAC QEAKKGVLIL SEFAGAAQSL
510 520 530 540 550
GAGAILVNPW NITEVAASIG QALNMTAEER EKRHRHNFHH VKTHTAQEWA
560 570 580 590 600
ETFVSELNDT VIEAQLRISK VPPELPQHDA IQRYSKSNNR LLILGFNATL
610 620 630 640 650
TEPVDNQGRR GDQIKEMDLN LHPELKGPLK ALCSDPSTTI VVLSGSSRSV
660 670 680 690 700
LDKNFGEYDM WLAAENGMFL RLTNGEWMTT MPEHLNMEWV DSVKHVFKYF
710 720 730 740 750
TERTPRSHFE TRDTSLIWNY KYADIEFGRL QARDLLQHLW TGPISNASVD
760 770 780 790 800
VVQGSRSVEV RAVGVTKGAA IDRILGEIVH SKSMTTPIDY VLCIGHFLGK
810 820 830 840 850
DEDVYTFFEP ELPSDMPAIA RSRPSSDSGA KSSSGDRRPP SKSTHNNNKS
860 870 880 890 900
GSKSSSSSNS NNNNKSSQRS LQSERKSGSN HSLGNSRRPS PEKISWNVLD
910 920 930 940
LKGENYFSCA VGRTRTNARY LLGSPDDVVC FLEKLADTTS SP
Length:942
Mass (Da):105,976
Last modified:May 1, 2000 - v1
Checksum:iB22AC11EA34CDDDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105A → P in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti348I → K in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti359 – 360AG → TD in CAA69879 (PubMed:9681010).Curated2
Sequence conflicti401Q → K in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti408T → P in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti415K → T in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti428N → I in CAA69879 (PubMed:9681010).Curated1
Sequence conflicti431F → L in CAA69879 (PubMed:9681010).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08568 mRNA Translation: CAA69879.1
AC007260 Genomic DNA Translation: AAD30578.1
CP002684 Genomic DNA Translation: AEE36123.1
CP002684 Genomic DNA Translation: ANM60660.1
CP002684 Genomic DNA Translation: ANM60661.1
CP002684 Genomic DNA Translation: ANM60662.1
AF370287 mRNA Translation: AAK44102.2
PIRiD96814
RefSeqiNP_001319403.1, NM_001334832.1
NP_001322931.1, NM_001334833.1
NP_001322932.1, NM_001334834.1
NP_177979.1, NM_106505.5
UniGeneiAt.198
At.71030

Genome annotation databases

EnsemblPlantsiAT1G78580.1; AT1G78580.1; AT1G78580
AT1G78580.2; AT1G78580.2; AT1G78580
AT1G78580.3; AT1G78580.3; AT1G78580
AT1G78580.4; AT1G78580.4; AT1G78580
GeneIDi844194
GrameneiAT1G78580.1; AT1G78580.1; AT1G78580
AT1G78580.2; AT1G78580.2; AT1G78580
AT1G78580.3; AT1G78580.3; AT1G78580
AT1G78580.4; AT1G78580.4; AT1G78580
KEGGiath:AT1G78580

Similar proteinsi

Entry informationi

Entry nameiTPS1_ARATH
AccessioniPrimary (citable) accession number: Q9SYM4
Secondary accession number(s): P93653, Q94K55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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