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Protein

B-box zinc finger protein 22

Gene

BBX22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as positive regulator of seedling photomorphogenesis and light-regulated inhibition of hypocotyl elongation, independently and in concert with HY5 and BBX21 (PubMed:18540109, PubMed:18796637, PubMed:18182030, PubMed:21427283). Acts as a positive regulator of de-etiolation and influences chloroplast biogenesis and function through regulation of genes encoding chloroplast proteins (PubMed:18182030). Acts downstream of COP1 and play an important role in early and long-term adjustment of the shade avoidance syndrome (SAS) responses in natural environments (PubMed:21070414). Regulates the expression of genes responsive to light hormone signals which may contribute to optimal seedling development (PubMed:21427283).5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 4743B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri57 – 9943B box-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
B-box zinc finger protein 221 Publication
Alternative name(s):
Protein DOUBLE B-BOX 31 Publication
Protein LIGHT-REGULATED ZINC FINGER PROTEIN 11 Publication
Protein SALT TOLERANCE HOMOLOG 3
Gene namesi
Name:BBX221 Publication
Synonyms:DBB31 Publication, LZF11 Publication, STH3
Ordered Locus Names:At1g78600
ORF Names:T30F21.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G78600.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Increased hypocotyl length under short day conditions (PubMed:18796637). Delayed chloroplast development (PubMed:18182030).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201D → A: No effect on the interaction with HY5.
Mutagenesisi72 – 721D → A: Abolishes interaction with HY5. 1 Publication
Mutagenesisi81 – 811D → A: Abolishes interaction with HY5. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299B-box zinc finger protein 22PRO_0000113298Add
BLAST

Post-translational modificationi

Ubiquitinated by COP1 in vitro (PubMed:18796637). COP1-mediated degradation of BBX22 by the proteasome occurs in the dark and is important for a precise skotomorphogenesis process and optimization of seedling growth under short days conditions (PubMed:21427283).2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ9SYM2.

Expressioni

Inductioni

By light.1 Publication

Gene expression databases

ExpressionAtlasiQ9SYM2. baseline and differential.
GenevisibleiQ9SYM2. AT.

Interactioni

Subunit structurei

Interacts with HY5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HY5O246463EBI-1994217,EBI-301660

Protein-protein interaction databases

IntActiQ9SYM2. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9SYM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi149 – 1557Poly-Gln

Sequence similaritiesi

Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 4743B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri57 – 9943B box-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000239240.
InParanoidiQ9SYM2.
PhylomeDBiQ9SYM2.

Family and domain databases

InterProiIPR000315. Znf_B-box.
[Graphical view]
PfamiPF00643. zf-B_box. 2 hits.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
[Graphical view]
PROSITEiPS50119. ZF_BBOX. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SYM2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIQCNVCEA AEATVLCCAD EAALCWACDE KIHAANKLAG KHQRVPLSAS
60 70 80 90 100
ASSIPKCDIC QEASGFFFCL QDRALLCRKC DVAIHTVNPH VSAHQRFLLT
110 120 130 140 150
GIKVGLESID TGPSTKSSPT NDDKTMETKP FVQSIPEPQK MAFDHHHHQQ
160 170 180 190 200
QQEQQEGVIP GTKVNDQTST KLPLVSSGST TGSIPQWQIE EIFGLTDFDQ
210 220 230 240 250
SYEYMENNGS SKADTSRRGD SDSSSMMRSA EEDGEDNNNC LGGETSWAVP
260 270 280 290
QIQSPPTASG LNWPKHFHHH SVFVPDITSS TPYTGSSPNQ RVGKRRRRF
Length:299
Mass (Da):32,864
Last modified:September 27, 2004 - v2
Checksum:i0FDF266FE3CA451A
GO

Sequence cautioni

The sequence AAD30576.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007260 Genomic DNA. Translation: AAD30576.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36125.1.
AY093108 mRNA. Translation: AAM13107.1.
BT006324 mRNA. Translation: AAP13432.1.
AY087387 mRNA. Translation: AAM64937.1.
PIRiF96814.
RefSeqiNP_565183.1. NM_106507.3. [Q9SYM2-1]
UniGeneiAt.19261.

Genome annotation databases

EnsemblPlantsiAT1G78600.1; AT1G78600.1; AT1G78600. [Q9SYM2-1]
GeneIDi844196.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007260 Genomic DNA. Translation: AAD30576.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36125.1.
AY093108 mRNA. Translation: AAM13107.1.
BT006324 mRNA. Translation: AAP13432.1.
AY087387 mRNA. Translation: AAM64937.1.
PIRiF96814.
RefSeqiNP_565183.1. NM_106507.3. [Q9SYM2-1]
UniGeneiAt.19261.

3D structure databases

ProteinModelPortaliQ9SYM2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9SYM2. 5 interactions.

Proteomic databases

PRIDEiQ9SYM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G78600.1; AT1G78600.1; AT1G78600. [Q9SYM2-1]
GeneIDi844196.

Organism-specific databases

TAIRiAT1G78600.

Phylogenomic databases

HOGENOMiHOG000239240.
InParanoidiQ9SYM2.
PhylomeDBiQ9SYM2.

Miscellaneous databases

PROiQ9SYM2.

Gene expression databases

ExpressionAtlasiQ9SYM2. baseline and differential.
GenevisibleiQ9SYM2. AT.

Family and domain databases

InterProiIPR000315. Znf_B-box.
[Graphical view]
PfamiPF00643. zf-B_box. 2 hits.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
[Graphical view]
PROSITEiPS50119. ZF_BBOX. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The common function of a novel subfamily of B-Box zinc finger proteins with reference to circadian-associated events in Arabidopsis thaliana."
    Kumagai T., Ito S., Nakamichi N., Niwa Y., Murakami M., Yamashino T., Mizuno T.
    Biosci. Biotechnol. Biochem. 72:1539-1549(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-box protein involved in light-dependent development and gene expression, undergoes COP1-mediated ubiquitination."
    Datta S., Johansson H., Hettiarachchi C., Irigoyen M.L., Desai M., Rubio V., Holm M.
    Plant Cell 20:2324-2338(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HY5, SUBCELLULAR LOCATION, UBIQUITINATION BY COP1, MUTAGENESIS OF ASP-20; ASP-72 AND ASP-81, DISRUPTION PHENOTYPE.
  7. "LZF1, a HY5-regulated transcriptional factor, functions in Arabidopsis de-etiolation."
    Chang C.S., Li Y.H., Chen L.T., Chen W.C., Hsieh W.P., Shin J., Jane W.N., Chou S.J., Choi G., Hu J.M., Somerville S., Wu S.H.
    Plant J. 54:205-219(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT, DISRUPTION PHENOTYPE.
  8. Cited for: GENE FAMILY, NOMENCLATURE.
  9. "AtBBX21 and COP1 genetically interact in the regulation of shade avoidance."
    Crocco C.D., Holm M., Yanovsky M.J., Botto J.F.
    Plant J. 64:551-562(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "COP1-mediated degradation of BBX22/LZF1 optimizes seedling development in Arabidopsis."
    Chang C.S., Maloof J.N., Wu S.H.
    Plant Physiol. 156:228-239(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEGRADATION BY THE PROTEASOME.

Entry informationi

Entry nameiBBX22_ARATH
AccessioniPrimary (citable) accession number: Q9SYM2
Secondary accession number(s): Q8RWG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: September 27, 2004
Last modified: November 11, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.