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Q9SYK0

- HEXO2_ARATH

UniProt

Q9SYK0 - HEXO2_ARATH

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Protein

Beta-hexosaminidase 2

Gene

HEXO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity. Can use synthetic substrates such as p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO4) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Inhibited by N-acetylcastanospermine.1 Publication

Kineticsi

  1. KM=1.2 mM for pNP-GlcNAc (at pH 4.6 and 37 degrees Celsius)2 Publications

Vmax=7.9 µmol/min/mg enzyme with pNP-GlcNAc as substrate (at pH 4.6 and 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is 4-5.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371Proton donorBy similarity

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: TAIR
  2. hexosaminidase activity Source: TAIR
  3. UDP-glucosyltransferase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G05590-MONOMER.
ReactomeiREACT_187588. Hyaluronan uptake and degradation.
REACT_187659. Glycosphingolipid metabolism.
REACT_187663. Keratan sulfate degradation.
REACT_215132. CS/DS degradation.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase 2 (EC:3.2.1.52)
Alternative name(s):
Beta-GlcNAcase 2
Beta-N-acetylhexosaminidase 2
Beta-hexosaminidase 3
Short name:
AtHEX3
N-acetyl-beta-glucosaminidase 2
Gene namesi
Name:HEXO2
Synonyms:HEX3
Ordered Locus Names:At1g05590
ORF Names:F3F20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G05590.

Subcellular locationi

Cell membrane 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 580552Beta-hexosaminidase 2PRO_0000420287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi279 ↔ 342By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi447 – 4471N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SYK0.
PRIDEiQ9SYK0.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9SYK0.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G05590.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SYK0.
SMRiQ9SYK0. Positions 42-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3525.
HOGENOMiHOG000157972.
InParanoidiQ9SYK0.
KOiK12373.
OMAiTAVYWED.
PhylomeDBiQ9SYK0.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SYK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTLSKFHVI LIPILFFITL LSPLFSIALP INIWPKPRFL SWPQHKAIAL
60 70 80 90 100
SPNFTILAPE HQYLSASVTR YHNLIRSENY SPLISYPVKL MKRYTLRNLV
110 120 130 140 150
VTVTDFSLPL HHGVDESYKL SIPIGSFSAH LLAHSAWGAM RGLETFSQMI
160 170 180 190 200
WGTSPDLCLP VGIYIQDSPL FGHRGVLLDT SRNYYGVDDI MRTIKAMSAN
210 220 230 240 250
KLNVFHWHIT DSQSFPLVLP SEPSLAAKGS LGPDMVYTPE DVSKIVQYGF
260 270 280 290 300
EHGVRVLPEI DTPGHTGSWG EAYPEIVTCA NMFWWPAGKS WEERLASEPG
310 320 330 340 350
TGQLNPLSPK TYEVVKNVIQ DIVNQFPESF FHGGGDEVIP GCWKTDPAIN
360 370 380 390 400
SFLSSGGTLS QLLEKYINST LPYIVSQNRT VVYWEDVLLD AQIKADPSVL
410 420 430 440 450
PKEHTILQTW NNGPENTKRI VAAGYRVIVS SSEFYYLDCG HGGFLGNDSI
460 470 480 490 500
YDQKESGGGS WCAPFKTWQS IYNYDIADGL LNEEERKLVL GGEVALWSEQ
510 520 530 540 550
ADSTVLDSRL WPRASALAES LWSGNRDERG VKRCGEAVDR LNLWRYRMVK
560 570 580
RGIGAEPIQP LWCLKNPGMC NTVHGALQDQ
Length:580
Mass (Da):64,991
Last modified:May 1, 2000 - v1
Checksum:i4C1421A5CA71C853
GO

Sequence cautioni

The sequence AK229119 differs from that shown. Reason: Frameshift at position 240. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007153 Genomic DNA. Translation: AAD30612.1.
CP002684 Genomic DNA. Translation: AEE27861.1.
AK229119 mRNA. No translation available.
PIRiH86189.
RefSeqiNP_172050.1. NM_100439.2.
UniGeneiAt.42389.

Genome annotation databases

EnsemblPlantsiAT1G05590.1; AT1G05590.1; AT1G05590.
GeneIDi837064.
KEGGiath:AT1G05590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007153 Genomic DNA. Translation: AAD30612.1 .
CP002684 Genomic DNA. Translation: AEE27861.1 .
AK229119 mRNA. No translation available.
PIRi H86189.
RefSeqi NP_172050.1. NM_100439.2.
UniGenei At.42389.

3D structure databases

ProteinModelPortali Q9SYK0.
SMRi Q9SYK0. Positions 42-563.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G05590.1-P.

Protein family/group databases

CAZyi GH20. Glycoside Hydrolase Family 20.

Proteomic databases

PaxDbi Q9SYK0.
PRIDEi Q9SYK0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G05590.1 ; AT1G05590.1 ; AT1G05590 .
GeneIDi 837064.
KEGGi ath:AT1G05590.

Organism-specific databases

TAIRi AT1G05590.

Phylogenomic databases

eggNOGi COG3525.
HOGENOMi HOG000157972.
InParanoidi Q9SYK0.
KOi K12373.
OMAi TAVYWED.
PhylomeDBi Q9SYK0.

Enzyme and pathway databases

BioCyci ARA:AT1G05590-MONOMER.
Reactomei REACT_187588. Hyaluronan uptake and degradation.
REACT_187659. Glycosphingolipid metabolism.
REACT_187663. Keratan sulfate degradation.
REACT_215132. CS/DS degradation.

Gene expression databases

Genevestigatori Q9SYK0.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProi IPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view ]
Pfami PF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSi PR00738. GLHYDRLASE20.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Biosynthesis of truncated N-linked oligosaccharides results from non-orthologous hexosaminidase-mediated mechanisms in nematodes, plants, and insects."
    Gutternigg M., Kretschmer-Lubich D., Paschinger K., Rendic D., Hader J., Geier P., Ranftl R., Jantsch V., Lochnit G., Wilson I.B.H.
    J. Biol. Chem. 282:27825-27840(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, REVIEW.
  5. "Enzymatic properties and subcellular localization of Arabidopsis beta-N-acetylhexosaminidases."
    Strasser R., Bondili J.S., Schoberer J., Svoboda B., Liebminger E., Glossl J., Altmann F., Steinkellner H., Mach L.
    Plant Physiol. 145:5-16(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION, GENE FAMILY, NOMENCLATURE.
  6. "Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana."
    Liebminger E., Veit C., Pabst M., Batoux M., Zipfel C., Altmann F., Mach L., Strasser R.
    J. Biol. Chem. 286:10793-10802(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiHEXO2_ARATH
AccessioniPrimary (citable) accession number: Q9SYK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3