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Q9SYK0

- HEXO2_ARATH

UniProt

Q9SYK0 - HEXO2_ARATH

Protein

Beta-hexosaminidase 2

Gene

HEXO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Has a broad substrate specificity. Can use synthetic substrates such as p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO4) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference.3 Publications

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

    Enzyme regulationi

    Inhibited by N-acetylcastanospermine.1 Publication

    Kineticsi

    1. KM=1.2 mM for pNP-GlcNAc (at pH 4.6 and 37 degrees Celsius)2 Publications

    Vmax=7.9 µmol/min/mg enzyme with pNP-GlcNAc as substrate (at pH 4.6 and 37 degrees Celsius)2 Publications

    pH dependencei

    Optimum pH is 4-5.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei337 – 3371Proton donorBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: TAIR
    2. hexosaminidase activity Source: TAIR
    3. UDP-glucosyltransferase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G05590-MONOMER.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase 2 (EC:3.2.1.52)
    Alternative name(s):
    Beta-GlcNAcase 2
    Beta-N-acetylhexosaminidase 2
    Beta-hexosaminidase 3
    Short name:
    AtHEX3
    N-acetyl-beta-glucosaminidase 2
    Gene namesi
    Name:HEXO2
    Synonyms:HEX3
    Ordered Locus Names:At1g05590
    ORF Names:F3F20.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G05590.

    Subcellular locationi

    Cell membrane 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 580552Beta-hexosaminidase 2PRO_0000420287Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi279 ↔ 342By similarity
    Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi447 – 4471N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9SYK0.
    PRIDEiQ9SYK0.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9SYK0.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G05590.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SYK0.
    SMRiQ9SYK0. Positions 42-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    HOGENOMiHOG000157972.
    InParanoidiQ9SYK0.
    KOiK12373.
    OMAiTAVYWED.
    PhylomeDBiQ9SYK0.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SYK0-1 [UniParc]FASTAAdd to Basket

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    MLTLSKFHVI LIPILFFITL LSPLFSIALP INIWPKPRFL SWPQHKAIAL    50
    SPNFTILAPE HQYLSASVTR YHNLIRSENY SPLISYPVKL MKRYTLRNLV 100
    VTVTDFSLPL HHGVDESYKL SIPIGSFSAH LLAHSAWGAM RGLETFSQMI 150
    WGTSPDLCLP VGIYIQDSPL FGHRGVLLDT SRNYYGVDDI MRTIKAMSAN 200
    KLNVFHWHIT DSQSFPLVLP SEPSLAAKGS LGPDMVYTPE DVSKIVQYGF 250
    EHGVRVLPEI DTPGHTGSWG EAYPEIVTCA NMFWWPAGKS WEERLASEPG 300
    TGQLNPLSPK TYEVVKNVIQ DIVNQFPESF FHGGGDEVIP GCWKTDPAIN 350
    SFLSSGGTLS QLLEKYINST LPYIVSQNRT VVYWEDVLLD AQIKADPSVL 400
    PKEHTILQTW NNGPENTKRI VAAGYRVIVS SSEFYYLDCG HGGFLGNDSI 450
    YDQKESGGGS WCAPFKTWQS IYNYDIADGL LNEEERKLVL GGEVALWSEQ 500
    ADSTVLDSRL WPRASALAES LWSGNRDERG VKRCGEAVDR LNLWRYRMVK 550
    RGIGAEPIQP LWCLKNPGMC NTVHGALQDQ 580
    Length:580
    Mass (Da):64,991
    Last modified:May 1, 2000 - v1
    Checksum:i4C1421A5CA71C853
    GO

    Sequence cautioni

    The sequence AK229119 differs from that shown. Reason: Frameshift at position 240.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007153 Genomic DNA. Translation: AAD30612.1.
    CP002684 Genomic DNA. Translation: AEE27861.1.
    AK229119 mRNA. No translation available.
    PIRiH86189.
    RefSeqiNP_172050.1. NM_100439.2.
    UniGeneiAt.42389.

    Genome annotation databases

    EnsemblPlantsiAT1G05590.1; AT1G05590.1; AT1G05590.
    GeneIDi837064.
    KEGGiath:AT1G05590.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007153 Genomic DNA. Translation: AAD30612.1 .
    CP002684 Genomic DNA. Translation: AEE27861.1 .
    AK229119 mRNA. No translation available.
    PIRi H86189.
    RefSeqi NP_172050.1. NM_100439.2.
    UniGenei At.42389.

    3D structure databases

    ProteinModelPortali Q9SYK0.
    SMRi Q9SYK0. Positions 42-563.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G05590.1-P.

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    Proteomic databases

    PaxDbi Q9SYK0.
    PRIDEi Q9SYK0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G05590.1 ; AT1G05590.1 ; AT1G05590 .
    GeneIDi 837064.
    KEGGi ath:AT1G05590.

    Organism-specific databases

    TAIRi AT1G05590.

    Phylogenomic databases

    eggNOGi COG3525.
    HOGENOMi HOG000157972.
    InParanoidi Q9SYK0.
    KOi K12373.
    OMAi TAVYWED.
    PhylomeDBi Q9SYK0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G05590-MONOMER.

    Gene expression databases

    Genevestigatori Q9SYK0.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProi IPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view ]
    Pfami PF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSi PR00738. GLHYDRLASE20.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Biosynthesis of truncated N-linked oligosaccharides results from non-orthologous hexosaminidase-mediated mechanisms in nematodes, plants, and insects."
      Gutternigg M., Kretschmer-Lubich D., Paschinger K., Rendic D., Hader J., Geier P., Ranftl R., Jantsch V., Lochnit G., Wilson I.B.H.
      J. Biol. Chem. 282:27825-27840(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, REVIEW.
    5. "Enzymatic properties and subcellular localization of Arabidopsis beta-N-acetylhexosaminidases."
      Strasser R., Bondili J.S., Schoberer J., Svoboda B., Liebminger E., Glossl J., Altmann F., Steinkellner H., Mach L.
      Plant Physiol. 145:5-16(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION, GENE FAMILY, NOMENCLATURE.
    6. "Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana."
      Liebminger E., Veit C., Pabst M., Batoux M., Zipfel C., Altmann F., Mach L., Strasser R.
      J. Biol. Chem. 286:10793-10802(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiHEXO2_ARATH
    AccessioniPrimary (citable) accession number: Q9SYK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3