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Q9SYJ4 (RFS4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable galactinol--sucrose galactosyltransferase 4

EC=2.4.1.82
Alternative name(s):
Raffinose synthase 4
Gene names
Name:RFS4
Synonyms:RS4
Ordered Locus Names:At4g01970
ORF Names:T7B11.23
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

Induction

By oxidative stress. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Sequence caution

The sequence AAD22659.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB80690.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Probable galactinol--sucrose galactosyltransferase 4
PRO_0000389257

Amino acid modifications

Modified residue71Phosphoserine By similarity
Modified residue91Phosphoserine By similarity

Experimental info

Sequence conflict7921A → V in BAF00996. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9SYJ4 [UniParc].

Last modified May 3, 2011. Version 3.
Checksum: 9155965DD4EF0FAF

FASTA87698,012
        10         20         30         40         50         60 
MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL FDVPQNVTFT 

        70         80         90        100        110        120 
PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL TNSLGRFEDR EFLSLFRFKM 

       130        140        150        160        170        180 
WWSTAWIGKS GSDLQAETQW VMLKIPEIDS YVAIIPTIEG AFRASLTPGE KGNVLICAES 

       190        200        210        220        230        240 
GSTKVKESSF KSIAYIHICD NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW 

       250        260        270        280        290        300 
DACYLTVDPA TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT 

       310        320        330        340        350        360 
ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR RKLVKESGEQ 

       370        380        390        400        410        420 
DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA AFTKDLRLRF KSLDDIYVWH 

       430        440        450        460        470        480 
ALCGAWNGVR PETMMDLKAK VAPFELSPSL GATMADLAVD KVVEAGIGLV HPSKAHEFYD 

       490        500        510        520        530        540 
SMHSYLASVG VTGAKIDVFQ TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM 

       550        560        570        580        590        600 
QQCNEFFFLA TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD 

       610        620        630        640        650        660 
WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT IPRCVHYALP 

       670        680        690        700        710        720 
TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS PEEHRFKGYK ECYTTVSGTV 

       730        740        750        760        770        780 
HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ SEEILFMNSK SEAMKITLEP SAFDLLSFVP 

       790        800        810        820        830        840 
VTELVSSGVR FAPLGLINMF NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL 

       850        860        870 
NDKEAEFKWE EETGKLSFFV PWVEESGGIS HLSFTF 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
Nishizawa A., Yabuta Y., Shigeoka S.
Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007138 Genomic DNA. Translation: AAD22659.1. Sequence problems.
AL161493 Genomic DNA. Translation: CAB80690.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82106.1.
AK229121 mRNA. Translation: BAF00996.1.
PIRC85025.
RefSeqNP_192106.3. NM_116428.4.
UniGeneAt.34347.

3D structure databases

ProteinModelPortalQ9SYJ4.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbQ9SYJ4.
PRIDEQ9SYJ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G01970.1; AT4G01970.1; AT4G01970.
GeneID828186.
KEGGath:AT4G01970.

Organism-specific databases

TAIRAT4G01970.

Phylogenomic databases

eggNOGNOG318101.
HOGENOMHOG000237551.
InParanoidQ0WPF3.
KOK06611.
OMAKVDVIHT.

Family and domain databases

Gene3D3.20.20.70. 3 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
ProtoNetSearch...

Entry information

Entry nameRFS4_ARATH
AccessionPrimary (citable) accession number: Q9SYJ4
Secondary accession number(s): Q0WPF3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 3, 2011
Last modified: May 14, 2014
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names