Q9SYJ4 (RFS4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactinol--sucrose galactosyltransferase 4
EC=2.4.1.82

Alternative name(s):
Raffinose synthase 4

Gene names

Name:	RFS4
Synonyms:	RS4
Ordered Locus Names:	At4g01970
ORF Names:	T7B11.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.
Induction	By oxidative stress. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.
Sequence caution	The sequence AAD22659.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80690.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR response to oxidative stress Inferred from expression pattern Ref.4. Source: TAIR
Molecular_function	galactinol-raffinose galactosyltransferase activity Inferred from sequence or structural similarity PubMed 15034167. Source: TAIR galactinol-sucrose galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	Probable galactinol--sucrose galactosyltransferase 4		PRO_0000389257
Experimental info
	Sequence conflict	792	1	A → V in BAF00996. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9SYJ4 [UniParc]. Last modified May 3, 2011. Version 3. Checksum: 9155965DD4EF0FAF	FASTA	876	98,012
	10 20 30 40 50 60 MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL FDVPQNVTFT 70 80 90 100 110 120 PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL TNSLGRFEDR EFLSLFRFKM 130 140 150 160 170 180 WWSTAWIGKS GSDLQAETQW VMLKIPEIDS YVAIIPTIEG AFRASLTPGE KGNVLICAES 190 200 210 220 230 240 GSTKVKESSF KSIAYIHICD NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW 250 260 270 280 290 300 DACYLTVDPA TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT 310 320 330 340 350 360 ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR RKLVKESGEQ 370 380 390 400 410 420 DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA AFTKDLRLRF KSLDDIYVWH 430 440 450 460 470 480 ALCGAWNGVR PETMMDLKAK VAPFELSPSL GATMADLAVD KVVEAGIGLV HPSKAHEFYD 490 500 510 520 530 540 SMHSYLASVG VTGAKIDVFQ TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM 550 560 570 580 590 600 QQCNEFFFLA TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD 610 620 630 640 650 660 WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT IPRCVHYALP 670 680 690 700 710 720 TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS PEEHRFKGYK ECYTTVSGTV 730 740 750 760 770 780 HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ SEEILFMNSK SEAMKITLEP SAFDLLSFVP 790 800 810 820 830 840 VTELVSSGVR FAPLGLINMF NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL 850 860 870 NDKEAEFKWE EETGKLSFFV PWVEESGGIS HLSFTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage." Nishizawa A., Yabuta Y., Shigeoka S. Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007138 Genomic DNA. Translation: AAD22659.1. Sequence problems. AL161493 Genomic DNA. Translation: CAB80690.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE82106.1. AK229121 mRNA. Translation: BAF00996.1.
IPI	IPI00852301.
PIR	C85025.
RefSeq	NP_192106.3. NM_116428.4.
UniGene	At.34347.
3D structure databases
ProteinModelPortal	Q9SYJ4.
ModBase	Search...
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Proteomic databases
PaxDb	Q9SYJ4.
PRIDE	Q9SYJ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G01970.1; AT4G01970.1; AT4G01970.
GeneID	828186.
KEGG	ath:AT4G01970.
Organism-specific databases
TAIR	At4g01970.
Phylogenomic databases
eggNOG	NOG318101.
HOGENOM	HOG000237551.
InParanoid	Q0WPF3.
KO	K06611.
OMA	IASMQQC.
Family and domain databases
Gene3D	3.20.20.70. 3 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS4_ARATH

Accession

Primary (citable) accession number: Q9SYJ4
Secondary accession number(s): Q0WPF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	May 3, 2011
Last modified:	May 1, 2013
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program