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Q9SYJ4

- RFS4_ARATH

UniProt

Q9SYJ4 - RFS4_ARATH

Protein

Probable galactinol--sucrose galactosyltransferase 4

Gene

RFS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 3 (03 May 2011)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

    GO - Molecular functioni

    1. galactinol-raffinose galactosyltransferase activity Source: TAIR
    2. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. response to karrikin Source: TAIR
    3. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable galactinol--sucrose galactosyltransferase 4 (EC:2.4.1.82)
    Alternative name(s):
    Raffinose synthase 4
    Gene namesi
    Name:RFS4
    Synonyms:RS4
    Ordered Locus Names:At4g01970
    ORF Names:T7B11.23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G01970.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 876876Probable galactinol--sucrose galactosyltransferase 4PRO_0000389257Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei7 – 71PhosphoserineBy similarity
    Modified residuei9 – 91PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9SYJ4.
    PRIDEiQ9SYJ4.

    Expressioni

    Inductioni

    By oxidative stress.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SYJ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG318101.
    HOGENOMiHOG000237551.
    InParanoidiQ0WPF3.
    KOiK06611.
    OMAiKVDVIHT.

    Family and domain databases

    Gene3Di3.20.20.70. 3 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9SYJ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL    50
    FDVPQNVTFT PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL 100
    TNSLGRFEDR EFLSLFRFKM WWSTAWIGKS GSDLQAETQW VMLKIPEIDS 150
    YVAIIPTIEG AFRASLTPGE KGNVLICAES GSTKVKESSF KSIAYIHICD 200
    NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW DACYLTVDPA 250
    TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT 300
    ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR 350
    RKLVKESGEQ DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA 400
    AFTKDLRLRF KSLDDIYVWH ALCGAWNGVR PETMMDLKAK VAPFELSPSL 450
    GATMADLAVD KVVEAGIGLV HPSKAHEFYD SMHSYLASVG VTGAKIDVFQ 500
    TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM QQCNEFFFLA 550
    TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD 600
    WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT 650
    IPRCVHYALP TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS 700
    PEEHRFKGYK ECYTTVSGTV HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ 750
    SEEILFMNSK SEAMKITLEP SAFDLLSFVP VTELVSSGVR FAPLGLINMF 800
    NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL NDKEAEFKWE 850
    EETGKLSFFV PWVEESGGIS HLSFTF 876
    Length:876
    Mass (Da):98,012
    Last modified:May 3, 2011 - v3
    Checksum:i9155965DD4EF0FAF
    GO

    Sequence cautioni

    The sequence AAD22659.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80690.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti792 – 7921A → V in BAF00996. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007138 Genomic DNA. Translation: AAD22659.1. Sequence problems.
    AL161493 Genomic DNA. Translation: CAB80690.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82106.1.
    AK229121 mRNA. Translation: BAF00996.1.
    PIRiC85025.
    RefSeqiNP_192106.3. NM_116428.4.
    UniGeneiAt.34347.

    Genome annotation databases

    EnsemblPlantsiAT4G01970.1; AT4G01970.1; AT4G01970.
    GeneIDi828186.
    KEGGiath:AT4G01970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007138 Genomic DNA. Translation: AAD22659.1 . Sequence problems.
    AL161493 Genomic DNA. Translation: CAB80690.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82106.1 .
    AK229121 mRNA. Translation: BAF00996.1 .
    PIRi C85025.
    RefSeqi NP_192106.3. NM_116428.4.
    UniGenei At.34347.

    3D structure databases

    ProteinModelPortali Q9SYJ4.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PaxDbi Q9SYJ4.
    PRIDEi Q9SYJ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G01970.1 ; AT4G01970.1 ; AT4G01970 .
    GeneIDi 828186.
    KEGGi ath:AT4G01970.

    Organism-specific databases

    TAIRi AT4G01970.

    Phylogenomic databases

    eggNOGi NOG318101.
    HOGENOMi HOG000237551.
    InParanoidi Q0WPF3.
    KOi K06611.
    OMAi KVDVIHT.

    Family and domain databases

    Gene3Di 3.20.20.70. 3 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
      Nishizawa A., Yabuta Y., Shigeoka S.
      Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiRFS4_ARATH
    AccessioniPrimary (citable) accession number: Q9SYJ4
    Secondary accession number(s): Q0WPF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 65 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3