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Protein

Probable galactinol--sucrose galactosyltransferase 4

Gene

RFS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • response to karrikin Source: TAIR
  • response to oxidative stress Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.4.1.82 399

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 4 (EC:2.4.1.82)
Alternative name(s):
Raffinose synthase 4
Gene namesi
Name:RFS4
Synonyms:RS4
Ordered Locus Names:At4g01970
ORF Names:T7B11.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G01970
TAIRilocus:2141425 AT4G01970

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003892571 – 876Probable galactinol--sucrose galactosyltransferase 4Add BLAST876

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei9PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SYJ4

Expressioni

Inductioni

By oxidative stress.1 Publication

Gene expression databases

ExpressionAtlasiQ9SYJ4 baseline and differential
GenevisibleiQ9SYJ4 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G01970.1

Structurei

3D structure databases

ProteinModelPortaliQ9SYJ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiENOG410IKHD Eukaryota
ENOG410YCZ1 LUCA
HOGENOMiHOG000237551
InParanoidiQ9SYJ4
KOiK06611
OMAiAYNSMWM
OrthoDBiEOG093601VH

Family and domain databases

InterProiView protein in InterPro
IPR017853 Glycoside_hydrolase_SF
IPR008811 Glycosyl_hydrolases_36
PANTHERiPTHR31268 PTHR31268, 1 hit
PfamiView protein in Pfam
PF05691 Raffinose_syn, 1 hit
SUPFAMiSSF51445 SSF51445, 3 hits

Sequencei

Sequence statusi: Complete.

Q9SYJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL
60 70 80 90 100
FDVPQNVTFT PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL
110 120 130 140 150
TNSLGRFEDR EFLSLFRFKM WWSTAWIGKS GSDLQAETQW VMLKIPEIDS
160 170 180 190 200
YVAIIPTIEG AFRASLTPGE KGNVLICAES GSTKVKESSF KSIAYIHICD
210 220 230 240 250
NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW DACYLTVDPA
260 270 280 290 300
TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT
310 320 330 340 350
ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR
360 370 380 390 400
RKLVKESGEQ DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA
410 420 430 440 450
AFTKDLRLRF KSLDDIYVWH ALCGAWNGVR PETMMDLKAK VAPFELSPSL
460 470 480 490 500
GATMADLAVD KVVEAGIGLV HPSKAHEFYD SMHSYLASVG VTGAKIDVFQ
510 520 530 540 550
TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM QQCNEFFFLA
560 570 580 590 600
TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD
610 620 630 640 650
WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT
660 670 680 690 700
IPRCVHYALP TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS
710 720 730 740 750
PEEHRFKGYK ECYTTVSGTV HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ
760 770 780 790 800
SEEILFMNSK SEAMKITLEP SAFDLLSFVP VTELVSSGVR FAPLGLINMF
810 820 830 840 850
NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL NDKEAEFKWE
860 870
EETGKLSFFV PWVEESGGIS HLSFTF
Length:876
Mass (Da):98,012
Last modified:May 3, 2011 - v3
Checksum:i9155965DD4EF0FAF
GO

Sequence cautioni

The sequence AAD22659 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80690 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti792A → V in BAF00996 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007138 Genomic DNA Translation: AAD22659.1 Sequence problems.
AL161493 Genomic DNA Translation: CAB80690.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82106.1
AK229121 mRNA Translation: BAF00996.1
PIRiC85025
RefSeqiNP_192106.3, NM_116428.5
UniGeneiAt.34347

Genome annotation databases

EnsemblPlantsiAT4G01970.1; AT4G01970.1; AT4G01970
GeneIDi828186
GrameneiAT4G01970.1; AT4G01970.1; AT4G01970
KEGGiath:AT4G01970

Similar proteinsi

Entry informationi

Entry nameiRFS4_ARATH
AccessioniPrimary (citable) accession number: Q9SYJ4
Secondary accession number(s): Q0WPF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 87 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health