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Q9SYJ4

- RFS4_ARATH

UniProt

Q9SYJ4 - RFS4_ARATH

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Protein

Probable galactinol--sucrose galactosyltransferase 4

Gene

RFS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

  1. galactinol-raffinose galactosyltransferase activity Source: TAIR
  2. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. response to karrikin Source: TAIR
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 4 (EC:2.4.1.82)
Alternative name(s):
Raffinose synthase 4
Gene namesi
Name:RFS4
Synonyms:RS4
Ordered Locus Names:At4g01970
ORF Names:T7B11.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G01970.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Probable galactinol--sucrose galactosyltransferase 4PRO_0000389257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineBy similarity
Modified residuei9 – 91PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SYJ4.
PRIDEiQ9SYJ4.

Expressioni

Inductioni

By oxidative stress.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9SYJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiNOG318101.
HOGENOMiHOG000237551.
InParanoidiQ9SYJ4.
KOiK06611.
OMAiKVDVIHT.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9SYJ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL
60 70 80 90 100
FDVPQNVTFT PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL
110 120 130 140 150
TNSLGRFEDR EFLSLFRFKM WWSTAWIGKS GSDLQAETQW VMLKIPEIDS
160 170 180 190 200
YVAIIPTIEG AFRASLTPGE KGNVLICAES GSTKVKESSF KSIAYIHICD
210 220 230 240 250
NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW DACYLTVDPA
260 270 280 290 300
TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT
310 320 330 340 350
ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR
360 370 380 390 400
RKLVKESGEQ DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA
410 420 430 440 450
AFTKDLRLRF KSLDDIYVWH ALCGAWNGVR PETMMDLKAK VAPFELSPSL
460 470 480 490 500
GATMADLAVD KVVEAGIGLV HPSKAHEFYD SMHSYLASVG VTGAKIDVFQ
510 520 530 540 550
TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM QQCNEFFFLA
560 570 580 590 600
TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD
610 620 630 640 650
WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT
660 670 680 690 700
IPRCVHYALP TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS
710 720 730 740 750
PEEHRFKGYK ECYTTVSGTV HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ
760 770 780 790 800
SEEILFMNSK SEAMKITLEP SAFDLLSFVP VTELVSSGVR FAPLGLINMF
810 820 830 840 850
NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL NDKEAEFKWE
860 870
EETGKLSFFV PWVEESGGIS HLSFTF
Length:876
Mass (Da):98,012
Last modified:May 3, 2011 - v3
Checksum:i9155965DD4EF0FAF
GO

Sequence cautioni

The sequence AAD22659.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80690.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti792 – 7921A → V in BAF00996. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007138 Genomic DNA. Translation: AAD22659.1. Sequence problems.
AL161493 Genomic DNA. Translation: CAB80690.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82106.1.
AK229121 mRNA. Translation: BAF00996.1.
PIRiC85025.
RefSeqiNP_192106.3. NM_116428.4.
UniGeneiAt.34347.

Genome annotation databases

EnsemblPlantsiAT4G01970.1; AT4G01970.1; AT4G01970.
GeneIDi828186.
KEGGiath:AT4G01970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007138 Genomic DNA. Translation: AAD22659.1 . Sequence problems.
AL161493 Genomic DNA. Translation: CAB80690.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE82106.1 .
AK229121 mRNA. Translation: BAF00996.1 .
PIRi C85025.
RefSeqi NP_192106.3. NM_116428.4.
UniGenei At.34347.

3D structure databases

ProteinModelPortali Q9SYJ4.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbi Q9SYJ4.
PRIDEi Q9SYJ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G01970.1 ; AT4G01970.1 ; AT4G01970 .
GeneIDi 828186.
KEGGi ath:AT4G01970.

Organism-specific databases

TAIRi AT4G01970.

Phylogenomic databases

eggNOGi NOG318101.
HOGENOMi HOG000237551.
InParanoidi Q9SYJ4.
KOi K06611.
OMAi KVDVIHT.

Family and domain databases

Gene3Di 3.20.20.70. 3 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
    Nishizawa A., Yabuta Y., Shigeoka S.
    Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiRFS4_ARATH
AccessioniPrimary (citable) accession number: Q9SYJ4
Secondary accession number(s): Q0WPF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 3, 2011
Last modified: October 29, 2014
This is version 66 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3