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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase 3

Gene

LPAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. Has preference for C-18-CoA substrates compared to C-16-CoA substrates.

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable glycerol-3-phosphate acyltransferase 2 (GPAT2), Glycerol-3-phosphate acyltransferase, chloroplastic (ATS1), Glycerol-3-phosphate acyltransferase 1 (GPAT1), Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 7 (GPAT7), Probable glycerol-3-phosphate acyltransferase 8 (GPAT8), Glycerol-3-phosphate acyltransferase 5 (GPAT5)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (LPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (LPAT1), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (LPAT5), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 3 (CDS3), Phosphatidate cytidylyltransferase 4, chloroplastic (CDS4), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase 5, chloroplastic (CDS5), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS2)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G51260-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (EC:2.3.1.51)
Alternative name(s):
Lysophosphatidyl acyltransferase 3
Gene namesi
Name:LPAT3
Synonyms:LPAAT3
Ordered Locus Names:At1g51260
ORF Names:F11M15.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G51260.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence analysisAdd
BLAST
Transmembranei335 – 35521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3763761-acyl-sn-glycerol-3-phosphate acyltransferase 3PRO_0000208182Add
BLAST

Proteomic databases

PaxDbiQ9SYC8.
PRIDEiQ9SYC8.

PTM databases

iPTMnetiQ9SYC8.

Expressioni

Tissue specificityi

Predominantly expressed in pollen.1 Publication

Gene expression databases

GenevisibleiQ9SYC8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G51260.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SYC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi92 – 976HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000006110.
InParanoidiQ9SYC8.
KOiK13523.
OMAiPMPFWLA.
PhylomeDBiQ9SYC8.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SYC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIPAALVFI PVGVLFLISG LIVNIIQLVF FIIVRPFSRS LYRRINKNVA
60 70 80 90 100
ELLWLQLIWL FDWWACIKIN LYVDAETLEL IGKEHALVLS NHRSDIDWLI
110 120 130 140 150
GWVMAQRVGC LGSSLAIMKK EAKYLPIIGW SMWFSDYIFL ERSWAKDENT
160 170 180 190 200
LKAGFKRLED FPMTFWLALF VEGTRFTQEK LEAAQEYASI RSLPSPRNVL
210 220 230 240 250
IPRTKGFVSA VSEIRSFVPA IYDCTLTVHN NQPTPTLLRM FSGQSSEINL
260 270 280 290 300
QMRRHKMSEL PETDDGIAQW CQDLFITKDA QLEKYFTKDV FSDLEVHQIN
310 320 330 340 350
RPIKPLIVVI IWLGFLVFGG FKLLQWLSIV ASWKIILLFV FFLVIATITM
360 370
QILIQSSESQ RSTPAKRPLQ EQLISA
Length:376
Mass (Da):43,435
Last modified:May 1, 2000 - v1
Checksum:i0B9AE143B09ED4D0
GO

Sequence cautioni

The sequence BX813937 differs from that shown. Reason: Erroneous termination at position 325. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541I → S in BX813937 (PubMed:14993207).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006085 Genomic DNA. Translation: AAD30638.1.
CP002684 Genomic DNA. Translation: AEE32642.1.
BX813937 mRNA. No translation available.
PIRiD96550.
RefSeqiNP_175537.1. NM_104004.2.
UniGeneiAt.49969.

Genome annotation databases

EnsemblPlantsiAT1G51260.1; AT1G51260.1; AT1G51260.
GeneIDi841549.
GrameneiAT1G51260.1; AT1G51260.1; AT1G51260.
KEGGiath:AT1G51260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006085 Genomic DNA. Translation: AAD30638.1.
CP002684 Genomic DNA. Translation: AEE32642.1.
BX813937 mRNA. No translation available.
PIRiD96550.
RefSeqiNP_175537.1. NM_104004.2.
UniGeneiAt.49969.

3D structure databases

ProteinModelPortaliQ9SYC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G51260.1.

PTM databases

iPTMnetiQ9SYC8.

Proteomic databases

PaxDbiQ9SYC8.
PRIDEiQ9SYC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G51260.1; AT1G51260.1; AT1G51260.
GeneIDi841549.
GrameneiAT1G51260.1; AT1G51260.1; AT1G51260.
KEGGiath:AT1G51260.

Organism-specific databases

TAIRiAT1G51260.

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000006110.
InParanoidiQ9SYC8.
KOiK13523.
OMAiPMPFWLA.
PhylomeDBiQ9SYC8.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciARA:AT1G51260-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ9SYC8.

Gene expression databases

GenevisibleiQ9SYC8. AT.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Ubiquitous and endoplasmic reticulum-located lysophosphatidyl acyltransferase, LPAT2, is essential for female but not male gametophyte development in Arabidopsis."
    Kim H.U., Li Y., Huang A.H.C.
    Plant Cell 17:1073-1089(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLPAT3_ARATH
AccessioniPrimary (citable) accession number: Q9SYC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 2000
Last modified: February 17, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.