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Q9SY55

- ECA3_ARATH

UniProt

Q9SY55 - ECA3_ARATH

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Protein

Calcium-transporting ATPase 3, endoplasmic reticulum-type

Gene

ECA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment. Involved in calcium-enhanced root growth, in tolerance to toxic levels of manganese and in secretory processes. Has a crucial role in manganese nutrition, but is not involved in transporting copper, iron or zinc.2 Publications

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi300 – 3001Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi301 – 3011Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi303 – 3031Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi305 – 3051Calcium 2By similarity
Active sitei347 – 34714-aspartylphosphate intermediateBy similarity
Metal bindingi692 – 6921MagnesiumBy similarity
Metal bindingi696 – 6961MagnesiumBy similarity
Metal bindingi757 – 7571Calcium 1By similarity
Metal bindingi760 – 7601Calcium 1By similarity
Metal bindingi785 – 7851Calcium 2By similarity
Metal bindingi788 – 7881Calcium 1By similarity
Metal bindingi789 – 7891Calcium 1By similarity
Metal bindingi789 – 7891Calcium 2By similarity
Metal bindingi894 – 8941Calcium 1By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: TAIR
  3. manganese-transporting ATPase activity Source: TAIR
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: TAIR
  3. manganese ion homeostasis Source: TAIR
  4. manganese ion transmembrane transport Source: GOC
  5. manganese ion transport Source: TAIR
  6. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.

Protein family/group databases

TCDBi3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 3, endoplasmic reticulum-type (EC:3.6.3.8)
Short name:
AtECA3
Gene namesi
Name:ECA3
Synonyms:ACA6
Ordered Locus Names:At1g10130
ORF Names:F14N23.1, T27I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G10130.

Subcellular locationi

GO - Cellular componenti

  1. endomembrane system Source: TAIR
  2. endosome Source: TAIR
  3. Golgi apparatus Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 998998Calcium-transporting ATPase 3, endoplasmic reticulum-typePRO_0000046407Add
BLAST

Proteomic databases

PaxDbiQ9SY55.
PRIDEiQ9SY55.

Expressioni

Tissue specificityi

Expressed in root cap, in elongation and differentiation zones of roots, in vascular tissues of roots, leaves, floral pedicels and style, in leaves, including hydathodes and guard cells, in stamens, in petals, in sepals and in siliques.2 Publications

Inductioni

Not induced by manganese or zinc.1 Publication

Gene expression databases

ExpressionAtlasiQ9SY55. baseline and differential.
GenevestigatoriQ9SY55.

Structurei

3D structure databases

ProteinModelPortaliQ9SY55.
SMRiQ9SY55. Positions 1-976.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini70 – 8920LumenalSequence AnalysisAdd
BLAST
Topological domaini110 – 250141CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini271 – 29121LumenalSequence AnalysisAdd
BLAST
Topological domaini310 – 746437CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini767 – 77610LumenalSequence Analysis
Topological domaini798 – 81720CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini841 – 88343LumenalSequence AnalysisAdd
BLAST
Topological domaini904 – 91613CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini936 – 95015LumenalSequence AnalysisAdd
BLAST
Topological domaini972 – 99827CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 10920HelicalSequence AnalysisAdd
BLAST
Transmembranei251 – 27020HelicalSequence AnalysisAdd
BLAST
Transmembranei292 – 30918HelicalSequence AnalysisAdd
BLAST
Transmembranei747 – 76620HelicalSequence AnalysisAdd
BLAST
Transmembranei777 – 79721HelicalSequence AnalysisAdd
BLAST
Transmembranei818 – 84023HelicalSequence AnalysisAdd
BLAST
Transmembranei884 – 90320HelicalSequence AnalysisAdd
BLAST
Transmembranei917 – 93519HelicalSequence AnalysisAdd
BLAST
Transmembranei951 – 97121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265621.
InParanoidiQ9SY55.
KOiK01537.
OMAiNISHFAD.
PhylomeDBiQ9SY55.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SY55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDAYARSVS EVLDFFGVDP TKGLSDSQVV HHSRLYGRNV LPEEKRTPFW
60 70 80 90 100
KLVLKQFDDL LVKILIVAAI VSFVLALANG ETGLTAFLEP FVILLILAAN
110 120 130 140 150
AAVGVITETN AEKALEELRA YQANIATVLR NGCFSILPAT ELVPGDIVEV
160 170 180 190 200
TVGCKIPADL RMIEMSSNTF RVDQAILTGE SCSVEKDVDC TLTTNAVYQD
210 220 230 240 250
KKNILFSGTD VVAGRGRAVV IGVGSNTAMG SIHDSMLQTD DEATPLKKKL
260 270 280 290 300
DEFGSFLAKV IAGICVLVWV VNIGHFSDPS HGGFFKGAIH YFKIAVALAV
310 320 330 340 350
AAIPEGLPAV VTTCLALGTK KMARLNAIVR SLPSVETLGC TTVICSDKTG
360 370 380 390 400
TLTTNMMSVS KICVVQSAEH GPMINEFTVS GTTYAPEGTV FDSNGMQLDL
410 420 430 440 450
PAQSPCLHHL AMCSSLCNDS ILQYNPDKDS YEKIGESTEV ALRVLAEKVG
460 470 480 490 500
LPGFDSMPSA LNMLSKHERA SYCNHYWENQ FKKVYVLEFT RDRKMMSVLC
510 520 530 540 550
SHKQMDVMFS KGAPESIIAR CNKILCNGDG SVVPLTAAGR AELESRFYSF
560 570 580 590 600
GDETLRCLAL AFKTVPHGQQ TISYDNENDL TFIGLVGMLD PPREEVRDAM
610 620 630 640 650
LACMTAGIRV IVVTGDNKST AESLCRKIGA FDNLVDFSGM SYTASEFERL
660 670 680 690 700
PAVQQTLALR RMTLFSRVEP SHKRMLVEAL QKQNEVVAMT GDGVNDAPAL
710 720 730 740 750
KKADIGIAMG SGTAVAKSAS DMVLADDNFA SIVAAVAEGR AIYNNTKQFI
760 770 780 790 800
RYMISSNIGE VVCIFVAAVL GIPDTLAPVQ LLWVNLVTDG LPATAIGFNK
810 820 830 840 850
QDSDVMKAKP RKVGEAVVTG WLFFRYLVIG VYVGLATVAG FIWWFVYSDG
860 870 880 890 900
GPKLTYSELM NFETCALRET TYPCSIFEDR HPSTVAMTVL VVVEMFNALN
910 920 930 940 950
NLSENQSLLV ITPRSNLWLV GSIILTMLLH VLILYVHPLA VLFSVTPLSW
960 970 980 990
AEWTAVLYLS FPVIIIDELL KFLSRNTGMR FRFRLRKADL LPKDRRDK
Length:998
Mass (Da):109,060
Last modified:September 26, 2001 - v3
Checksum:i214C9865833BA7C0
GO

Sequence cautioni

The sequence AAD32863.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461R → G in CAA10660. (PubMed:10433975)Curated
Sequence conflicti55 – 562KQ → NS in AAD29961. 1 PublicationCurated
Sequence conflicti200 – 2001D → N in AAT68271. (PubMed:18567829)Curated
Sequence conflicti378 – 3814TVSG → LLVE in AAD29961. 1 PublicationCurated
Sequence conflicti411 – 4111A → T in AAT68271. (PubMed:18567829)Curated
Sequence conflicti549 – 5491S → R in CAA10660. (PubMed:10433975)Curated
Sequence conflicti586 – 5861Missing in AAT68271. (PubMed:18567829)Curated
Sequence conflicti747 – 7471K → R in AAD29961. 1 PublicationCurated
Sequence conflicti943 – 9442FS → CA in AAD29961. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117296 mRNA. Translation: AAD29961.1.
AJ132388 mRNA. Translation: CAA10660.1.
EU082212 mRNA. Translation: ABU53680.1.
AY650902 mRNA. Translation: AAT68271.1.
AC004122 Genomic DNA. Translation: AAC34328.2.
AC005489 Genomic DNA. Translation: AAD32863.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1.
AK229199 mRNA. Translation: BAF01069.1.
PIRiT00633.
T52581.
RefSeqiNP_563860.1. NM_100887.2.
UniGeneiAt.24768.

Genome annotation databases

EnsemblPlantsiAT1G10130.1; AT1G10130.1; AT1G10130.
GeneIDi837550.
KEGGiath:AT1G10130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117296 mRNA. Translation: AAD29961.1 .
AJ132388 mRNA. Translation: CAA10660.1 .
EU082212 mRNA. Translation: ABU53680.1 .
AY650902 mRNA. Translation: AAT68271.1 .
AC004122 Genomic DNA. Translation: AAC34328.2 .
AC005489 Genomic DNA. Translation: AAD32863.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1 .
AK229199 mRNA. Translation: BAF01069.1 .
PIRi T00633.
T52581.
RefSeqi NP_563860.1. NM_100887.2.
UniGenei At.24768.

3D structure databases

ProteinModelPortali Q9SY55.
SMRi Q9SY55. Positions 1-976.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbi Q9SY55.
PRIDEi Q9SY55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G10130.1 ; AT1G10130.1 ; AT1G10130 .
GeneIDi 837550.
KEGGi ath:AT1G10130.

Organism-specific databases

TAIRi AT1G10130.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265621.
InParanoidi Q9SY55.
KOi K01537.
OMAi NISHFAD.
PhylomeDBi Q9SY55.

Enzyme and pathway databases

BioCyci ARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.

Gene expression databases

ExpressionAtlasi Q9SY55. baseline and differential.
Genevestigatori Q9SY55.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtECA3 encodes a homolog of endoplasmic reticulum-type Ca2+-ATPase from Arabidopsis thaliana."
    Liang F., Sze H.
    Plant Gene Register PGR99-077
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling.
  2. "Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist."
    Pittman J.K., Mills R.F., O'Connor C.D., Williams L.E.
    Gene 236:137-147(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  3. "ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis."
    Mills R.F., Doherty M.L., Lopez-Marques R.L., Weimar T., Dupree P., Palmgren M.G., Pittman J.K., Williams L.E.
    Plant Physiol. 146:116-128(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  4. "A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process."
    Li X., Chanroj S., Wu Z., Romanowsky S.M., Harper J.F., Sze H.
    Plant Physiol. 147:1675-1689(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiECA3_ARATH
AccessioniPrimary (citable) accession number: Q9SY55
Secondary accession number(s): Q0WP80
, Q6DQH3, Q9SAV5, Q9SMX2, Q9SWS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

ECA3 is functionally distinct from ECA1 and is localized to a separate compartment.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3