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Protein

Calcium-transporting ATPase 3, endoplasmic reticulum-type

Gene

ECA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment. Involved in calcium-enhanced root growth, in tolerance to toxic levels of manganese and in secretory processes. Has a crucial role in manganese nutrition, but is not involved in transporting copper, iron or zinc.2 Publications

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi301Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi303Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi305Calcium 2By similarity1
Active sitei3474-aspartylphosphate intermediateBy similarity1
Metal bindingi692MagnesiumBy similarity1
Metal bindingi696MagnesiumBy similarity1
Metal bindingi757Calcium 1By similarity1
Metal bindingi760Calcium 1By similarity1
Metal bindingi785Calcium 2By similarity1
Metal bindingi788Calcium 1By similarity1
Metal bindingi789Calcium 1By similarity1
Metal bindingi789Calcium 2By similarity1
Metal bindingi894Calcium 1By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • manganese-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion transport Source: TAIR
  • cellular calcium ion homeostasis Source: GO_Central
  • manganese ion homeostasis Source: TAIR
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.
BRENDAi3.6.3.8. 399.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 3, endoplasmic reticulum-type (EC:3.6.3.8)
Short name:
AtECA3
Gene namesi
Name:ECA3
Synonyms:ACA6
Ordered Locus Names:At1g10130
ORF Names:F14N23.1, T27I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10130.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 89LumenalSequence analysisAdd BLAST20
Transmembranei90 – 109HelicalSequence analysisAdd BLAST20
Topological domaini110 – 250CytoplasmicSequence analysisAdd BLAST141
Transmembranei251 – 270HelicalSequence analysisAdd BLAST20
Topological domaini271 – 291LumenalSequence analysisAdd BLAST21
Transmembranei292 – 309HelicalSequence analysisAdd BLAST18
Topological domaini310 – 746CytoplasmicSequence analysisAdd BLAST437
Transmembranei747 – 766HelicalSequence analysisAdd BLAST20
Topological domaini767 – 776LumenalSequence analysis10
Transmembranei777 – 797HelicalSequence analysisAdd BLAST21
Topological domaini798 – 817CytoplasmicSequence analysisAdd BLAST20
Transmembranei818 – 840HelicalSequence analysisAdd BLAST23
Topological domaini841 – 883LumenalSequence analysisAdd BLAST43
Transmembranei884 – 903HelicalSequence analysisAdd BLAST20
Topological domaini904 – 916CytoplasmicSequence analysisAdd BLAST13
Transmembranei917 – 935HelicalSequence analysisAdd BLAST19
Topological domaini936 – 950LumenalSequence analysisAdd BLAST15
Transmembranei951 – 971HelicalSequence analysisAdd BLAST21
Topological domaini972 – 998CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

  • endomembrane system Source: TAIR
  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • late endosome membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464071 – 998Calcium-transporting ATPase 3, endoplasmic reticulum-typeAdd BLAST998

Proteomic databases

PaxDbiQ9SY55.
PRIDEiQ9SY55.

Expressioni

Tissue specificityi

Expressed in root cap, in elongation and differentiation zones of roots, in vascular tissues of roots, leaves, floral pedicels and style, in leaves, including hydathodes and guard cells, in stamens, in petals, in sepals and in siliques.2 Publications

Inductioni

Not induced by manganese or zinc.1 Publication

Gene expression databases

GenevisibleiQ9SY55. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G10130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SY55.
SMRiQ9SY55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiQ9SY55.
KOiK01537.
OMAiISSWLFC.
OrthoDBiEOG093600SX.
PhylomeDBiQ9SY55.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SY55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDAYARSVS EVLDFFGVDP TKGLSDSQVV HHSRLYGRNV LPEEKRTPFW
60 70 80 90 100
KLVLKQFDDL LVKILIVAAI VSFVLALANG ETGLTAFLEP FVILLILAAN
110 120 130 140 150
AAVGVITETN AEKALEELRA YQANIATVLR NGCFSILPAT ELVPGDIVEV
160 170 180 190 200
TVGCKIPADL RMIEMSSNTF RVDQAILTGE SCSVEKDVDC TLTTNAVYQD
210 220 230 240 250
KKNILFSGTD VVAGRGRAVV IGVGSNTAMG SIHDSMLQTD DEATPLKKKL
260 270 280 290 300
DEFGSFLAKV IAGICVLVWV VNIGHFSDPS HGGFFKGAIH YFKIAVALAV
310 320 330 340 350
AAIPEGLPAV VTTCLALGTK KMARLNAIVR SLPSVETLGC TTVICSDKTG
360 370 380 390 400
TLTTNMMSVS KICVVQSAEH GPMINEFTVS GTTYAPEGTV FDSNGMQLDL
410 420 430 440 450
PAQSPCLHHL AMCSSLCNDS ILQYNPDKDS YEKIGESTEV ALRVLAEKVG
460 470 480 490 500
LPGFDSMPSA LNMLSKHERA SYCNHYWENQ FKKVYVLEFT RDRKMMSVLC
510 520 530 540 550
SHKQMDVMFS KGAPESIIAR CNKILCNGDG SVVPLTAAGR AELESRFYSF
560 570 580 590 600
GDETLRCLAL AFKTVPHGQQ TISYDNENDL TFIGLVGMLD PPREEVRDAM
610 620 630 640 650
LACMTAGIRV IVVTGDNKST AESLCRKIGA FDNLVDFSGM SYTASEFERL
660 670 680 690 700
PAVQQTLALR RMTLFSRVEP SHKRMLVEAL QKQNEVVAMT GDGVNDAPAL
710 720 730 740 750
KKADIGIAMG SGTAVAKSAS DMVLADDNFA SIVAAVAEGR AIYNNTKQFI
760 770 780 790 800
RYMISSNIGE VVCIFVAAVL GIPDTLAPVQ LLWVNLVTDG LPATAIGFNK
810 820 830 840 850
QDSDVMKAKP RKVGEAVVTG WLFFRYLVIG VYVGLATVAG FIWWFVYSDG
860 870 880 890 900
GPKLTYSELM NFETCALRET TYPCSIFEDR HPSTVAMTVL VVVEMFNALN
910 920 930 940 950
NLSENQSLLV ITPRSNLWLV GSIILTMLLH VLILYVHPLA VLFSVTPLSW
960 970 980 990
AEWTAVLYLS FPVIIIDELL KFLSRNTGMR FRFRLRKADL LPKDRRDK
Length:998
Mass (Da):109,060
Last modified:September 26, 2001 - v3
Checksum:i214C9865833BA7C0
GO

Sequence cautioni

The sequence AAD32863 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46R → G in CAA10660 (PubMed:10433975).Curated1
Sequence conflicti55 – 56KQ → NS in AAD29961 (Ref. 1) Curated2
Sequence conflicti200D → N in AAT68271 (PubMed:18567829).Curated1
Sequence conflicti378 – 381TVSG → LLVE in AAD29961 (Ref. 1) Curated4
Sequence conflicti411A → T in AAT68271 (PubMed:18567829).Curated1
Sequence conflicti549S → R in CAA10660 (PubMed:10433975).Curated1
Sequence conflicti586Missing in AAT68271 (PubMed:18567829).Curated1
Sequence conflicti747K → R in AAD29961 (Ref. 1) Curated1
Sequence conflicti943 – 944FS → CA in AAD29961 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117296 mRNA. Translation: AAD29961.1.
AJ132388 mRNA. Translation: CAA10660.1.
EU082212 mRNA. Translation: ABU53680.1.
AY650902 mRNA. Translation: AAT68271.1.
AC004122 Genomic DNA. Translation: AAC34328.2.
AC005489 Genomic DNA. Translation: AAD32863.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1.
AK229199 mRNA. Translation: BAF01069.1.
PIRiT00633.
T52581.
RefSeqiNP_563860.1. NM_100887.3.
UniGeneiAt.24768.

Genome annotation databases

EnsemblPlantsiAT1G10130.1; AT1G10130.1; AT1G10130.
GeneIDi837550.
GrameneiAT1G10130.1; AT1G10130.1; AT1G10130.
KEGGiath:AT1G10130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117296 mRNA. Translation: AAD29961.1.
AJ132388 mRNA. Translation: CAA10660.1.
EU082212 mRNA. Translation: ABU53680.1.
AY650902 mRNA. Translation: AAT68271.1.
AC004122 Genomic DNA. Translation: AAC34328.2.
AC005489 Genomic DNA. Translation: AAD32863.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1.
AK229199 mRNA. Translation: BAF01069.1.
PIRiT00633.
T52581.
RefSeqiNP_563860.1. NM_100887.3.
UniGeneiAt.24768.

3D structure databases

ProteinModelPortaliQ9SY55.
SMRiQ9SY55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G10130.1.

Protein family/group databases

TCDBi3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiQ9SY55.
PRIDEiQ9SY55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10130.1; AT1G10130.1; AT1G10130.
GeneIDi837550.
GrameneiAT1G10130.1; AT1G10130.1; AT1G10130.
KEGGiath:AT1G10130.

Organism-specific databases

TAIRiAT1G10130.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiQ9SY55.
KOiK01537.
OMAiISSWLFC.
OrthoDBiEOG093600SX.
PhylomeDBiQ9SY55.

Enzyme and pathway databases

BioCyciARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.
BRENDAi3.6.3.8. 399.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9SY55.

Gene expression databases

GenevisibleiQ9SY55. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECA3_ARATH
AccessioniPrimary (citable) accession number: Q9SY55
Secondary accession number(s): Q0WP80
, Q6DQH3, Q9SAV5, Q9SMX2, Q9SWS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 26, 2001
Last modified: November 30, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

ECA3 is functionally distinct from ECA1 and is localized to a separate compartment.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.