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Q9SY55

- ECA3_ARATH

UniProt

Q9SY55 - ECA3_ARATH

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Protein

Calcium-transporting ATPase 3, endoplasmic reticulum-type

Gene
ECA3, ACA6, At1g10130, F14N23.1, T27I1.16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment. Involved in calcium-enhanced root growth, in tolerance to toxic levels of manganese and in secretory processes. Has a crucial role in manganese nutrition, but is not involved in transporting copper, iron or zinc.2 Publications

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi300 – 3001Calcium 2; via carbonyl oxygen By similarity
Metal bindingi301 – 3011Calcium 2; via carbonyl oxygen By similarity
Metal bindingi303 – 3031Calcium 2; via carbonyl oxygen By similarity
Metal bindingi305 – 3051Calcium 2 By similarity
Active sitei347 – 34714-aspartylphosphate intermediate By similarity
Metal bindingi692 – 6921Magnesium By similarity
Metal bindingi696 – 6961Magnesium By similarity
Metal bindingi757 – 7571Calcium 1 By similarity
Metal bindingi760 – 7601Calcium 1 By similarity
Metal bindingi785 – 7851Calcium 2 By similarity
Metal bindingi788 – 7881Calcium 1 By similarity
Metal bindingi789 – 7891Calcium 1 By similarity
Metal bindingi789 – 7891Calcium 2 By similarity
Metal bindingi894 – 8941Calcium 1 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: TAIR
  3. manganese-transporting ATPase activity Source: TAIR
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: TAIR
  3. manganese ion homeostasis Source: TAIR
  4. manganese ion transmembrane transport Source: GOC
  5. manganese ion transport Source: TAIR
  6. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.

Protein family/group databases

TCDBi3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 3, endoplasmic reticulum-type (EC:3.6.3.8)
Short name:
AtECA3
Gene namesi
Name:ECA3
Synonyms:ACA6
Ordered Locus Names:At1g10130
ORF Names:F14N23.1, T27I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G10130.

Subcellular locationi

Endosome membrane; Multi-pass membrane protein. Prevacuolar compartment membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei49 – 6921Helical; Reviewed predictionAdd
BLAST
Topological domaini70 – 8920Lumenal Reviewed predictionAdd
BLAST
Transmembranei90 – 10920Helical; Reviewed predictionAdd
BLAST
Topological domaini110 – 250141Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei251 – 27020Helical; Reviewed predictionAdd
BLAST
Topological domaini271 – 29121Lumenal Reviewed predictionAdd
BLAST
Transmembranei292 – 30918Helical; Reviewed predictionAdd
BLAST
Topological domaini310 – 746437Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei747 – 76620Helical; Reviewed predictionAdd
BLAST
Topological domaini767 – 77610Lumenal Reviewed prediction
Transmembranei777 – 79721Helical; Reviewed predictionAdd
BLAST
Topological domaini798 – 81720Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei818 – 84023Helical; Reviewed predictionAdd
BLAST
Topological domaini841 – 88343Lumenal Reviewed predictionAdd
BLAST
Transmembranei884 – 90320Helical; Reviewed predictionAdd
BLAST
Topological domaini904 – 91613Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei917 – 93519Helical; Reviewed predictionAdd
BLAST
Topological domaini936 – 95015Lumenal Reviewed predictionAdd
BLAST
Transmembranei951 – 97121Helical; Reviewed predictionAdd
BLAST
Topological domaini972 – 99827Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endomembrane system Source: TAIR
  2. endosome Source: TAIR
  3. Golgi apparatus Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. late endosome membrane Source: UniProtKB-SubCell
  6. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 998998Calcium-transporting ATPase 3, endoplasmic reticulum-typePRO_0000046407Add
BLAST

Proteomic databases

PaxDbiQ9SY55.
PRIDEiQ9SY55.

Expressioni

Tissue specificityi

Expressed in root cap, in elongation and differentiation zones of roots, in vascular tissues of roots, leaves, floral pedicels and style, in leaves, including hydathodes and guard cells, in stamens, in petals, in sepals and in siliques.2 Publications

Inductioni

Not induced by manganese or zinc.1 Publication

Gene expression databases

GenevestigatoriQ9SY55.

Structurei

3D structure databases

ProteinModelPortaliQ9SY55.
SMRiQ9SY55. Positions 1-976.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265621.
InParanoidiQ9SY55.
KOiK01537.
OMAiNISHFAD.
PhylomeDBiQ9SY55.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SY55-1 [UniParc]FASTAAdd to Basket

« Hide

MEDAYARSVS EVLDFFGVDP TKGLSDSQVV HHSRLYGRNV LPEEKRTPFW    50
KLVLKQFDDL LVKILIVAAI VSFVLALANG ETGLTAFLEP FVILLILAAN 100
AAVGVITETN AEKALEELRA YQANIATVLR NGCFSILPAT ELVPGDIVEV 150
TVGCKIPADL RMIEMSSNTF RVDQAILTGE SCSVEKDVDC TLTTNAVYQD 200
KKNILFSGTD VVAGRGRAVV IGVGSNTAMG SIHDSMLQTD DEATPLKKKL 250
DEFGSFLAKV IAGICVLVWV VNIGHFSDPS HGGFFKGAIH YFKIAVALAV 300
AAIPEGLPAV VTTCLALGTK KMARLNAIVR SLPSVETLGC TTVICSDKTG 350
TLTTNMMSVS KICVVQSAEH GPMINEFTVS GTTYAPEGTV FDSNGMQLDL 400
PAQSPCLHHL AMCSSLCNDS ILQYNPDKDS YEKIGESTEV ALRVLAEKVG 450
LPGFDSMPSA LNMLSKHERA SYCNHYWENQ FKKVYVLEFT RDRKMMSVLC 500
SHKQMDVMFS KGAPESIIAR CNKILCNGDG SVVPLTAAGR AELESRFYSF 550
GDETLRCLAL AFKTVPHGQQ TISYDNENDL TFIGLVGMLD PPREEVRDAM 600
LACMTAGIRV IVVTGDNKST AESLCRKIGA FDNLVDFSGM SYTASEFERL 650
PAVQQTLALR RMTLFSRVEP SHKRMLVEAL QKQNEVVAMT GDGVNDAPAL 700
KKADIGIAMG SGTAVAKSAS DMVLADDNFA SIVAAVAEGR AIYNNTKQFI 750
RYMISSNIGE VVCIFVAAVL GIPDTLAPVQ LLWVNLVTDG LPATAIGFNK 800
QDSDVMKAKP RKVGEAVVTG WLFFRYLVIG VYVGLATVAG FIWWFVYSDG 850
GPKLTYSELM NFETCALRET TYPCSIFEDR HPSTVAMTVL VVVEMFNALN 900
NLSENQSLLV ITPRSNLWLV GSIILTMLLH VLILYVHPLA VLFSVTPLSW 950
AEWTAVLYLS FPVIIIDELL KFLSRNTGMR FRFRLRKADL LPKDRRDK 998
Length:998
Mass (Da):109,060
Last modified:September 26, 2001 - v3
Checksum:i214C9865833BA7C0
GO

Sequence cautioni

The sequence AAD32863.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461R → G in CAA10660. 1 Publication
Sequence conflicti55 – 562KQ → NS in AAD29961. 1 Publication
Sequence conflicti200 – 2001D → N in AAT68271. 1 Publication
Sequence conflicti378 – 3814TVSG → LLVE in AAD29961. 1 Publication
Sequence conflicti411 – 4111A → T in AAT68271. 1 Publication
Sequence conflicti549 – 5491S → R in CAA10660. 1 Publication
Sequence conflicti586 – 5861Missing in AAT68271. 1 Publication
Sequence conflicti747 – 7471K → R in AAD29961. 1 Publication
Sequence conflicti943 – 9442FS → CA in AAD29961. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117296 mRNA. Translation: AAD29961.1.
AJ132388 mRNA. Translation: CAA10660.1.
EU082212 mRNA. Translation: ABU53680.1.
AY650902 mRNA. Translation: AAT68271.1.
AC004122 Genomic DNA. Translation: AAC34328.2.
AC005489 Genomic DNA. Translation: AAD32863.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1.
AK229199 mRNA. Translation: BAF01069.1.
PIRiT00633.
T52581.
RefSeqiNP_563860.1. NM_100887.2.
UniGeneiAt.24768.

Genome annotation databases

EnsemblPlantsiAT1G10130.1; AT1G10130.1; AT1G10130.
GeneIDi837550.
KEGGiath:AT1G10130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117296 mRNA. Translation: AAD29961.1 .
AJ132388 mRNA. Translation: CAA10660.1 .
EU082212 mRNA. Translation: ABU53680.1 .
AY650902 mRNA. Translation: AAT68271.1 .
AC004122 Genomic DNA. Translation: AAC34328.2 .
AC005489 Genomic DNA. Translation: AAD32863.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28545.1 .
AK229199 mRNA. Translation: BAF01069.1 .
PIRi T00633.
T52581.
RefSeqi NP_563860.1. NM_100887.2.
UniGenei At.24768.

3D structure databases

ProteinModelPortali Q9SY55.
SMRi Q9SY55. Positions 1-976.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbi Q9SY55.
PRIDEi Q9SY55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G10130.1 ; AT1G10130.1 ; AT1G10130 .
GeneIDi 837550.
KEGGi ath:AT1G10130.

Organism-specific databases

TAIRi AT1G10130.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265621.
InParanoidi Q9SY55.
KOi K01537.
OMAi NISHFAD.
PhylomeDBi Q9SY55.

Enzyme and pathway databases

BioCyci ARA:AT1G10130-MONOMER.
MetaCyc:MONOMER-14610.

Gene expression databases

Genevestigatori Q9SY55.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtECA3 encodes a homolog of endoplasmic reticulum-type Ca2+-ATPase from Arabidopsis thaliana."
    Liang F., Sze H.
    Plant Gene Register PGR99-077
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling.
  2. "Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist."
    Pittman J.K., Mills R.F., O'Connor C.D., Williams L.E.
    Gene 236:137-147(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  3. "ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis."
    Mills R.F., Doherty M.L., Lopez-Marques R.L., Weimar T., Dupree P., Palmgren M.G., Pittman J.K., Williams L.E.
    Plant Physiol. 146:116-128(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  4. "A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process."
    Li X., Chanroj S., Wu Z., Romanowsky S.M., Harper J.F., Sze H.
    Plant Physiol. 147:1675-1689(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiECA3_ARATH
AccessioniPrimary (citable) accession number: Q9SY55
Secondary accession number(s): Q0WP80
, Q6DQH3, Q9SAV5, Q9SMX2, Q9SWS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 26, 2001
Last modified: May 14, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

ECA3 is functionally distinct from ECA1 and is localized to a separate compartment.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi