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Q9SY55

- ECA3_ARATH

UniProt

Q9SY55 - ECA3_ARATH

Protein

Calcium-transporting ATPase 3, endoplasmic reticulum-type

Gene

ECA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment. Involved in calcium-enhanced root growth, in tolerance to toxic levels of manganese and in secretory processes. Has a crucial role in manganese nutrition, but is not involved in transporting copper, iron or zinc.2 Publications

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi300 – 3001Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi301 – 3011Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi303 – 3031Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi305 – 3051Calcium 2By similarity
    Active sitei347 – 34714-aspartylphosphate intermediateBy similarity
    Metal bindingi692 – 6921MagnesiumBy similarity
    Metal bindingi696 – 6961MagnesiumBy similarity
    Metal bindingi757 – 7571Calcium 1By similarity
    Metal bindingi760 – 7601Calcium 1By similarity
    Metal bindingi785 – 7851Calcium 2By similarity
    Metal bindingi788 – 7881Calcium 1By similarity
    Metal bindingi789 – 7891Calcium 1By similarity
    Metal bindingi789 – 7891Calcium 2By similarity
    Metal bindingi894 – 8941Calcium 1By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: TAIR
    3. manganese-transporting ATPase activity Source: TAIR
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. calcium ion transmembrane transport Source: GOC
    2. calcium ion transport Source: TAIR
    3. manganese ion homeostasis Source: TAIR
    4. manganese ion transmembrane transport Source: GOC
    5. manganese ion transport Source: TAIR
    6. root development Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G10130-MONOMER.
    MetaCyc:MONOMER-14610.

    Protein family/group databases

    TCDBi3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-transporting ATPase 3, endoplasmic reticulum-type (EC:3.6.3.8)
    Short name:
    AtECA3
    Gene namesi
    Name:ECA3
    Synonyms:ACA6
    Ordered Locus Names:At1g10130
    ORF Names:F14N23.1, T27I1.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G10130.

    Subcellular locationi

    GO - Cellular componenti

    1. endomembrane system Source: TAIR
    2. endosome Source: TAIR
    3. Golgi apparatus Source: TAIR
    4. integral component of membrane Source: UniProtKB-KW
    5. late endosome membrane Source: UniProtKB-SubCell
    6. trans-Golgi network Source: TAIR

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 998998Calcium-transporting ATPase 3, endoplasmic reticulum-typePRO_0000046407Add
    BLAST

    Proteomic databases

    PaxDbiQ9SY55.
    PRIDEiQ9SY55.

    Expressioni

    Tissue specificityi

    Expressed in root cap, in elongation and differentiation zones of roots, in vascular tissues of roots, leaves, floral pedicels and style, in leaves, including hydathodes and guard cells, in stamens, in petals, in sepals and in siliques.2 Publications

    Inductioni

    Not induced by manganese or zinc.1 Publication

    Gene expression databases

    GenevestigatoriQ9SY55.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SY55.
    SMRiQ9SY55. Positions 1-976.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini70 – 8920LumenalSequence AnalysisAdd
    BLAST
    Topological domaini110 – 250141CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini271 – 29121LumenalSequence AnalysisAdd
    BLAST
    Topological domaini310 – 746437CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini767 – 77610LumenalSequence Analysis
    Topological domaini798 – 81720CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini841 – 88343LumenalSequence AnalysisAdd
    BLAST
    Topological domaini904 – 91613CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini936 – 95015LumenalSequence AnalysisAdd
    BLAST
    Topological domaini972 – 99827CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei49 – 6921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei90 – 10920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei251 – 27020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 30918HelicalSequence AnalysisAdd
    BLAST
    Transmembranei747 – 76620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei777 – 79721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei818 – 84023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei884 – 90320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei917 – 93519HelicalSequence AnalysisAdd
    BLAST
    Transmembranei951 – 97121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265621.
    InParanoidiQ9SY55.
    KOiK01537.
    OMAiNISHFAD.
    PhylomeDBiQ9SY55.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SY55-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDAYARSVS EVLDFFGVDP TKGLSDSQVV HHSRLYGRNV LPEEKRTPFW    50
    KLVLKQFDDL LVKILIVAAI VSFVLALANG ETGLTAFLEP FVILLILAAN 100
    AAVGVITETN AEKALEELRA YQANIATVLR NGCFSILPAT ELVPGDIVEV 150
    TVGCKIPADL RMIEMSSNTF RVDQAILTGE SCSVEKDVDC TLTTNAVYQD 200
    KKNILFSGTD VVAGRGRAVV IGVGSNTAMG SIHDSMLQTD DEATPLKKKL 250
    DEFGSFLAKV IAGICVLVWV VNIGHFSDPS HGGFFKGAIH YFKIAVALAV 300
    AAIPEGLPAV VTTCLALGTK KMARLNAIVR SLPSVETLGC TTVICSDKTG 350
    TLTTNMMSVS KICVVQSAEH GPMINEFTVS GTTYAPEGTV FDSNGMQLDL 400
    PAQSPCLHHL AMCSSLCNDS ILQYNPDKDS YEKIGESTEV ALRVLAEKVG 450
    LPGFDSMPSA LNMLSKHERA SYCNHYWENQ FKKVYVLEFT RDRKMMSVLC 500
    SHKQMDVMFS KGAPESIIAR CNKILCNGDG SVVPLTAAGR AELESRFYSF 550
    GDETLRCLAL AFKTVPHGQQ TISYDNENDL TFIGLVGMLD PPREEVRDAM 600
    LACMTAGIRV IVVTGDNKST AESLCRKIGA FDNLVDFSGM SYTASEFERL 650
    PAVQQTLALR RMTLFSRVEP SHKRMLVEAL QKQNEVVAMT GDGVNDAPAL 700
    KKADIGIAMG SGTAVAKSAS DMVLADDNFA SIVAAVAEGR AIYNNTKQFI 750
    RYMISSNIGE VVCIFVAAVL GIPDTLAPVQ LLWVNLVTDG LPATAIGFNK 800
    QDSDVMKAKP RKVGEAVVTG WLFFRYLVIG VYVGLATVAG FIWWFVYSDG 850
    GPKLTYSELM NFETCALRET TYPCSIFEDR HPSTVAMTVL VVVEMFNALN 900
    NLSENQSLLV ITPRSNLWLV GSIILTMLLH VLILYVHPLA VLFSVTPLSW 950
    AEWTAVLYLS FPVIIIDELL KFLSRNTGMR FRFRLRKADL LPKDRRDK 998
    Length:998
    Mass (Da):109,060
    Last modified:September 26, 2001 - v3
    Checksum:i214C9865833BA7C0
    GO

    Sequence cautioni

    The sequence AAD32863.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461R → G in CAA10660. (PubMed:10433975)Curated
    Sequence conflicti55 – 562KQ → NS in AAD29961. 1 PublicationCurated
    Sequence conflicti200 – 2001D → N in AAT68271. (PubMed:18567829)Curated
    Sequence conflicti378 – 3814TVSG → LLVE in AAD29961. 1 PublicationCurated
    Sequence conflicti411 – 4111A → T in AAT68271. (PubMed:18567829)Curated
    Sequence conflicti549 – 5491S → R in CAA10660. (PubMed:10433975)Curated
    Sequence conflicti586 – 5861Missing in AAT68271. (PubMed:18567829)Curated
    Sequence conflicti747 – 7471K → R in AAD29961. 1 PublicationCurated
    Sequence conflicti943 – 9442FS → CA in AAD29961. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117296 mRNA. Translation: AAD29961.1.
    AJ132388 mRNA. Translation: CAA10660.1.
    EU082212 mRNA. Translation: ABU53680.1.
    AY650902 mRNA. Translation: AAT68271.1.
    AC004122 Genomic DNA. Translation: AAC34328.2.
    AC005489 Genomic DNA. Translation: AAD32863.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28545.1.
    AK229199 mRNA. Translation: BAF01069.1.
    PIRiT00633.
    T52581.
    RefSeqiNP_563860.1. NM_100887.2.
    UniGeneiAt.24768.

    Genome annotation databases

    EnsemblPlantsiAT1G10130.1; AT1G10130.1; AT1G10130.
    GeneIDi837550.
    KEGGiath:AT1G10130.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117296 mRNA. Translation: AAD29961.1 .
    AJ132388 mRNA. Translation: CAA10660.1 .
    EU082212 mRNA. Translation: ABU53680.1 .
    AY650902 mRNA. Translation: AAT68271.1 .
    AC004122 Genomic DNA. Translation: AAC34328.2 .
    AC005489 Genomic DNA. Translation: AAD32863.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28545.1 .
    AK229199 mRNA. Translation: BAF01069.1 .
    PIRi T00633.
    T52581.
    RefSeqi NP_563860.1. NM_100887.2.
    UniGenei At.24768.

    3D structure databases

    ProteinModelPortali Q9SY55.
    SMRi Q9SY55. Positions 1-976.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 3.A.3.2.19. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    PaxDbi Q9SY55.
    PRIDEi Q9SY55.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G10130.1 ; AT1G10130.1 ; AT1G10130 .
    GeneIDi 837550.
    KEGGi ath:AT1G10130.

    Organism-specific databases

    TAIRi AT1G10130.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265621.
    InParanoidi Q9SY55.
    KOi K01537.
    OMAi NISHFAD.
    PhylomeDBi Q9SY55.

    Enzyme and pathway databases

    BioCyci ARA:AT1G10130-MONOMER.
    MetaCyc:MONOMER-14610.

    Gene expression databases

    Genevestigatori Q9SY55.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AtECA3 encodes a homolog of endoplasmic reticulum-type Ca2+-ATPase from Arabidopsis thaliana."
      Liang F., Sze H.
      Plant Gene Register PGR99-077
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Seedling.
    2. "Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist."
      Pittman J.K., Mills R.F., O'Connor C.D., Williams L.E.
      Gene 236:137-147(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Landsberg erecta.
    3. "ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis."
      Mills R.F., Doherty M.L., Lopez-Marques R.L., Weimar T., Dupree P., Palmgren M.G., Pittman J.K., Williams L.E.
      Plant Physiol. 146:116-128(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    4. "A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process."
      Li X., Chanroj S., Wu Z., Romanowsky S.M., Harper J.F., Sze H.
      Plant Physiol. 147:1675-1689(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    6. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiECA3_ARATH
    AccessioniPrimary (citable) accession number: Q9SY55
    Secondary accession number(s): Q0WP80
    , Q6DQH3, Q9SAV5, Q9SMX2, Q9SWS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ECA3 is functionally distinct from ECA1 and is localized to a separate compartment.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3