Q9SXV6 (CAS1_GLYGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cycloartenol synthase
EC=5.4.99.8

Gene names

Name:

GgCAS1

Organism

Glycyrrhiza glabra (Licorice)

Taxonomic identifier

49827 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Galegeae › Glycyrrhiza

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase converting oxidosqualene to cycloartenol. Required for the production of sterols. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = cycloartenol. Ref.1
Tissue specificity	Expressed in thickened roots and root nodules. Ref.2
Developmental stage	Constant levels of expression during germination and throughout the year. Ref.2
Induction	No effect of methyl jasmonate (MeJA) or gibberellin A3 (GA3). Ref.2
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 5 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	cycloartenol synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	Cycloartenol synthase		PRO_0000413992
Regions
	Repeat	147 – 188	42	PFTB 1
	Repeat	512 – 557	46	PFTB 2
	Repeat	589 – 629	41	PFTB 3
	Repeat	638 – 679	42	PFTB 4
	Repeat	700 – 741	42	PFTB 5
Sites
	Active site	257	1	Proton acceptor By similarity
	Active site	483	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SXV6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 396BBAEB1C2A2447	FASTA	757	86,454
	10 20 30 40 50 60 MWKLKIAEGG SPWLRTVNNH VGRQVWEFDP KLGSPEDLLE IEKARQNFHD NRFTHKHSAD 70 80 90 100 110 120 LLMRIHFAKE NPMNEVLPKV RVKDIEDVTE ETVKTTLRRA INFHSTLQSH DGHWPGDYGG 130 140 150 160 170 180 PMFLMPGLVI TLSITGALNA VLTEEHRKEI CRYLYNHQNK DGGWGLHIEG PSTMFGSVLN 190 200 210 220 230 240 YVALRLLGEG PNDRQGEMEK GRDWILGHGG ATFITSWGKM WLSVLGVYEW SGNNPLPPEI 250 260 270 280 290 300 WLLPYVLPIH PGRMWCHCRM VYLPMSYLYG KRFVGPITPT ILSLRKELYT IPYHDIDWNQ 310 320 330 340 350 360 ARNLCAKEDL YYPHPLVQDI LWASLHKFLE PILMHWPGKK LREMAIKTAI EHIHYEDDNT 370 380 390 400 410 420 RYLCIGPVNK VLNMLCCWVE DPNSEAFKLH LPRIYDYLWI AEDGMKMQGY NGSQLWDTAF 430 440 450 460 470 480 TAQAIISSNL IEEYGPTLRK AHTYIKNSQV LEDCPGDLSK WYRHISKGAW PFSTADHGWP 490 500 510 520 530 540 ISDCTAEGLK AVLLLSKIAP EIVGEPLDAK RLYDAVNVIL SLQNEDGGFA TYELTRSYTW 550 560 570 580 590 600 LELINPAETF GDIVIDYPYV ECTSAAIQAL TSFKKLYPGH RREEIQCCIE KAASFIEKTQ 610 620 630 640 650 660 ASDGSWYGSW GVCFTYGTWF GVKGLIAAGK SFNNCSSIRK ACEFLLSKQL PSGGWGESYL 670 680 690 700 710 720 SCQNKVYSNV ESNRSHVVNT GWAMLALIDA EQAKRDPTPL HRAAVYLINS QMENGDFPQQ 730 740 750 EIMGVFNKNC MITYAAYRNV FPIWALGEYR HRVLQSQ

References

[1]	"Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra cycloartenol synthase." Hayashi H., Hiraoka N., Ikeshiro Y., Kushiro T., Morita M., Shibuya M., Ebizuka Y. Biol. Pharm. Bull. 23:231-234(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
[2]	"Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra." Hayashi H., Huang P., Takada S., Obinata M., Inoue K., Shibuya M., Ebizuka Y. Biol. Pharm. Bull. 27:1086-1092(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025968 mRNA. Translation: BAA76902.1.
3D structure databases
ProteinModelPortal	Q9SXV6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	5.4.99.8. 265378.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 3 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAS1_GLYGL

Accession

Primary (citable) accession number: Q9SXV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program