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Protein

Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2

Gene

CYSD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a cysteine synthase. The cysteine synthesis reaction is more efficient than the cyanoalanine synthase activity.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication
L-cysteine + hydrogen cyanide = L-3-cyanoalanine + hydrogen sulfide.1 Publication

Cofactori

Kineticsi

  1. KM=7.97 mM for O3-acetyl-L-serine for the cysteine synthase activity1 Publication
  2. KM=0.25 mM for Na2S for the cysteine synthase activity1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
    2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei79Pyridoxal phosphateBy similarity1
    Binding sitei271Pyridoxal phosphateBy similarity1

    GO - Molecular functioni

    • cysteine synthase activity Source: TAIR
    • L-3-cyanoalanine synthase activity Source: UniProtKB-EC
    • pyridoxal phosphate binding Source: GO_Central

    GO - Biological processi

    Keywordsi

    Molecular functionLyase, Transferase
    Biological processAmino-acid biosynthesis, Cysteine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:AT5G28020-MONOMER
    ReactomeiR-ATH-1614603 Cysteine formation from homocysteine
    UniPathwayiUPA00136; UER00200

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (EC:2.5.1.471 Publication, EC:4.4.1.91 Publication)
    Alternative name(s):
    Beta-substituted Ala synthase 4;2
    Short name:
    ARAth-Bsas4;2
    Cysteine synthase D2
    Short name:
    AtcysD2
    O-acetylserine (thiol)-lyase 6
    Gene namesi
    Name:CYSD2
    Synonyms:OAS6
    Ordered Locus Names:At5g28020
    ORF Names:F15F15.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G28020
    TAIRilocus:2143814 AT5G28020

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004244591 – 323Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2Add BLAST323

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei48N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiQ9SXS7
    PRIDEiQ9SXS7

    Expressioni

    Tissue specificityi

    Predominantly expressed in leaves and flowers.1 Publication

    Inductioni

    By nitrogen starvation.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SXS7 baseline and differential
    GenevisibleiQ9SXS7 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi18146, 2 interactors
    IntActiQ9SXS7, 1 interactor
    STRINGi3702.AT5G28020.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SXS7
    SMRiQ9SXS7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni183 – 187Pyridoxal phosphate bindingBy similarity5

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1252 Eukaryota
    COG0031 LUCA
    HOGENOMiHOG000217394
    InParanoidiQ9SXS7
    KOiK01738
    OMAiYAKLEWY
    OrthoDBiEOG09360FNG
    PhylomeDBiQ9SXS7

    Family and domain databases

    InterProiView protein in InterPro
    IPR005856 Cys_synth
    IPR005859 CysK
    IPR001216 P-phosphate_BS
    IPR001926 PLP-dep
    IPR036052 Trypto_synt_PLP_dependent
    PfamiView protein in Pfam
    PF00291 PALP, 1 hit
    SUPFAMiSSF53686 SSF53686, 1 hit
    TIGRFAMsiTIGR01139 cysK, 1 hit
    TIGR01136 cysKM, 1 hit
    PROSITEiView protein in PROSITE
    PS00901 CYS_SYNTHASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9SXS7-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEDRCLIKND ITELIGNTPM VYLNNVVDGC VARIAAKLEM MEPCSSVKDR
    60 70 80 90 100
    IAYSMIKDAE DKGLITPGKS TLIEPTAGNT GIGLACMGAA RGYKVILVMP
    110 120 130 140 150
    STMSLERRII LRALGAELHL SDQRIGLKGM LEKTEAILSK TPGGYIPQQF
    160 170 180 190 200
    ENPANPEIHY RTTGPEIWRD SAGKVDILVA GVGTGGTATG VGKFLKEQNK
    210 220 230 240 250
    DIKVCVVEPV ESPVLSGGQP GPHLIQGIGS GIVPFNLDLT IVDEIIQVAG
    260 270 280 290 300
    EEAIETAKLL ALKEGLLVGI SSGAAAAAAL KVAKRPENAG KLIVVVFPSG
    310 320
    GERYLSTKLF DSIRYEAENL PIE
    Length:323
    Mass (Da):34,318
    Last modified:May 1, 2000 - v1
    Checksum:i877C8677BEA777F4
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti11 – 15ITELI → VTELV in AAM62728 (Ref. 6) Curated5
    Sequence conflicti26V → I in BAH20170 (PubMed:19423640).Curated1
    Sequence conflicti61D → E in AAM62728 (Ref. 6) Curated1
    Sequence conflicti117E → D in AAM62728 (Ref. 6) Curated1
    Sequence conflicti148Q → R in CAB55622 (PubMed:10889265).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024283 mRNA Translation: BAA78561.1
    AC007627 Genomic DNA No translation available.
    CP002688 Genomic DNA Translation: AED93759.1
    CP002688 Genomic DNA Translation: AED93760.1
    CP002688 Genomic DNA Translation: AED93761.1
    CP002688 Genomic DNA Translation: AED93762.1
    CP002688 Genomic DNA Translation: AED93764.1
    AF424598 mRNA Translation: AAL11592.1
    AY124831 mRNA Translation: AAM70540.1
    AK317505 mRNA Translation: BAH20170.1
    AY085504 mRNA Translation: AAM62728.1
    AJ011044 mRNA Translation: CAB55622.1
    PIRiT52661
    RefSeqiNP_001031956.1, NM_001036879.2 [Q9SXS7-1]
    NP_001031957.1, NM_001036880.3 [Q9SXS7-1]
    NP_001078628.1, NM_001085159.1 [Q9SXS7-1]
    NP_198154.1, NM_122685.3 [Q9SXS7-1]
    NP_851087.1, NM_180756.3 [Q9SXS7-1]
    UniGeneiAt.23896

    Genome annotation databases

    EnsemblPlantsiAT5G28020.1; AT5G28020.1; AT5G28020 [Q9SXS7-1]
    AT5G28020.2; AT5G28020.2; AT5G28020 [Q9SXS7-1]
    AT5G28020.3; AT5G28020.3; AT5G28020 [Q9SXS7-1]
    AT5G28020.4; AT5G28020.4; AT5G28020 [Q9SXS7-1]
    AT5G28020.6; AT5G28020.6; AT5G28020 [Q9SXS7-1]
    GeneIDi832872
    GrameneiAT5G28020.1; AT5G28020.1; AT5G28020 [Q9SXS7-1]
    AT5G28020.2; AT5G28020.2; AT5G28020 [Q9SXS7-1]
    AT5G28020.3; AT5G28020.3; AT5G28020 [Q9SXS7-1]
    AT5G28020.4; AT5G28020.4; AT5G28020 [Q9SXS7-1]
    AT5G28020.6; AT5G28020.6; AT5G28020 [Q9SXS7-1]
    KEGGiath:AT5G28020

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiCYSD2_ARATH
    AccessioniPrimary (citable) accession number: Q9SXS7
    Secondary accession number(s): B9DHF3, Q8LEC3, Q9SMY4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: May 1, 2000
    Last modified: May 23, 2018
    This is version 127 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

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