Reviewed, UniProtKB/Swiss-Prot Q9SXP2 (FDH1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase 1, mitochondrial
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase 1
      Short name=FDH 1

Gene names

Ordered Locus Names:	Os06g0486800, LOC_Os06g29180
ORF Names:	P0008F02.23, P0404G03.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 20	20	Mitochondrion Ref.3
	Chain	21 – 376	356	Formate dehydrogenase 1, mitochondrial		PRO_0000007195
Sites
	Active site	282	1	By similarity
	Active site	330	1	Proton donor By similarity
Experimental info
	Sequence conflict	316 – 317	2	DH → GP in BAA77337. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SXP2-1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 9FE964AE572C585B	FASTA	376	41,341
	10 20 30 40 50 60 MAMWRAAAGH LLGRALGSRA AHTSAGSKKI VGVFYKGGEY ADKNPNFVGC VEGALGIREW 70 80 90 100 110 120 LESKGHHYIV TDDKEGLNSE LEKHIEDMHV LITTPFHPAY VSAERIKKAK NLELLLTAGI 130 140 150 160 170 180 GSDHIDLPAA AAAGLTVAEV TGSNTVSVAE DELMRILILL RNFLPGYQQV VHGEWNVAGI 190 200 210 220 230 240 AYRAYDLEGK TVGTVGAGRI GRLLLQRLKP FNCNLLYHDR LKIDPELEKE IGAKYEEDLD 250 260 270 280 290 300 AMLPKCDVIV INTPLTEKTR GMFNKERIAK MKKGVIIVNN ARGAIMDTQA VADACSSGQV 310 320 330 340 350 360 AGYGGDVWFP QPAPKDHPWR YMPNHAMTPH ISGTTIDAQL RYAAGVKDML DRYFKGEDFP 370 VQNYIVKEGQ LASQYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAD-dependent formate dehydrogenase." Shiraishi T., Fukusaki E., Kobayashi A. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-30. Strain: cv. Nipponbare. Tissue: Callus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB019533 mRNA. Translation: BAA77337.1. AP003518 Genomic DNA. Translation: BAD37348.1. AP005656 Genomic DNA. Translation: BAD38299.1.
RefSeq	NP_001057666.1.
UniGene	Os.4156
3D structure databases
HSSP	HSSP built from PDB template 2NAD based on UniProtKB P33160.
ModBase	Search...
Genome annotation databases
GeneID	4341069.
KEGG	osa:4341069.
Organism-specific databases
Gramene	Q9SXP2.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH1_ORYSJ

Accession

Primary (citable) accession number: Q9SXP2
Secondary accession number(s): Q67U72

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)