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Protein

Histidine kinase 1

Gene

AHK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an osmosensor histidine kinase that detects water stress and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Positive regulator of drought and salt stress responses, and abscisic acid (ABA) signaling. Confers drought tolerance, probably by regulating levels of ABA accumulation. Plays a redundant role in regulating plant growth and development. Required for the regulation of desiccation processes during seed formation.3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: TAIR
  • osmosensor activity Source: TAIR
  • phosphorelay sensor kinase activity Source: InterPro

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of ethylene-activated signaling pathway Source: GO_Central
  • response to osmotic stress Source: TAIR
  • response to water deprivation Source: TAIR
  • seed maturation Source: TAIR
  • stomatal complex patterning Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Abscisic acid signaling pathway, Two-component regulatory system

Enzyme and pathway databases

BioCyciARA:AT2G17820-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine kinase 1 (EC:2.7.13.3)
Alternative name(s):
Arabidopsis histidine kinase 1
Short name:
AtHK1
Protein AUTHENTIC HIS-KINASE 1
Gene namesi
Name:AHK1
Ordered Locus Names:At2g17820
ORF Names:T13L16.16, T17A5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G17820.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8181CytoplasmicSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 446344ExtracellularSequence analysisAdd
BLAST
Transmembranei447 – 46721HelicalSequence analysisAdd
BLAST
Topological domaini468 – 1207740CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Insensitivity to ABA, but hypersensitivity to drought and high salinity stresses. Loss of seed viability over time dur to altered dessiccation.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi508 – 5081H → V: Loss of histidine kinase activity. 1 Publication
Mutagenesisi1075 – 10751D → E: Loss of histidine kinase activity and impaired interaction with AHP2. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12071207Histidine kinase 1PRO_0000398586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei508 – 5081Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei1127 – 112714-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated predominantly on His residues. Activation probably requires a transfer of a phosphate group between a His in the transmitter domain and an Asp of the receiver domain (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SXL4.
PRIDEiQ9SXL4.

Expressioni

Tissue specificityi

Mostly expressed in roots, and, to a lower extent, in stems, leaves and flowers.1 Publication

Inductioni

Up-regulated in response to changes in external osmolarity, low temperature, cytokinin (CK) treatment, and dehydration.2 Publications

Gene expression databases

GenevisibleiQ9SXL4. AT.

Interactioni

Subunit structurei

Interacts with AHP2, depending of the phosphorylation state of Asp-1075 in the receiver domain, but probably not with AHP1 and AHP3.1 Publication

Protein-protein interaction databases

BioGridi1648. 2 interactions.
IntActiQ9SXL4. 1 interaction.
STRINGi3702.AT2G17820.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SXL4.
SMRiQ9SXL4. Positions 499-753, 1044-1195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini505 – 763259Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini1045 – 1196152Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRDA. Eukaryota.
ENOG410XT1K. LUCA.
HOGENOMiHOG000029544.
InParanoidiQ9SXL4.
OMAiWHFTRIY.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 2 hits.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SXL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGDSFSMSI ENLPDSPMGS RKKKMQIRKV FDKMTEWVTP WRSNLESPRE
60 70 80 90 100
MMILRGDVEQ DEFQYASSHC LSSYYSVFVV RLAIMVMLAI LIGLLTVLTW
110 120 130 140 150
HFTRIYTKQS LQTLAYGLRY ELLQRPVLRM WSVLNTTSEL TTAQVKLSEY
160 170 180 190 200
VIKKYDKPTT QEELVEMYQA MKDVTWALFA SAKALNAITI NYRNGFVQAF
210 220 230 240 250
HRDPASSSTF YIFSDLKNYS ISGTGPEDVS GWNNKSIHGN MSAIWYQQQL
260 270 280 290 300
DPVTGENLGK PLKIPPDDLI NIAGISQVPD GEASWHVTVS KYMDSPLLSA
310 320 330 340 350
ALPVFDASNK SIVAVVGVTT ALYSVGQLMR DLVEVHGGHI YLTSQEGYLL
360 370 380 390 400
ATSTDGPLLK NTSNGPQLMK ATDSEEWVIK SGAQWLEKTY GSKRPHVVHA
410 420 430 440 450
ENVKLGDQRY YIDSFYLNLK RLPIVGVVII PRKFIMGKVD ERAFKTLIIL
460 470 480 490 500
ISASVCIFFI GCVCILILTN GVSKEMKLRA ELIRQLDARR RAEASSNYKS
510 520 530 540 550
QFLANMSHEL RTPMAAVIGL LDILISDDCL SNEQYATVTQ IRKCSTALLR
560 570 580 590 600
LLNNILDLSK VESGKLVLEE AEFDLGRELE GLVDMFSVQC INHNVETVLD
610 620 630 640 650
LSDDMPALVR GDSARLVQIF ANLISNSIKF TTTGHIILRG WCENINSLHD
660 670 680 690 700
EMSVSVDRRK PWAPMKTKQV QHRNHLQKSC KNANKMVLWF EVDDTGCGID
710 720 730 740 750
PSKWDSVFES FEQADPSTTR THGGTGLGLC IVRNLVNKMG GEIKVVQKNG
760 770 780 790 800
LGTLMRLYLI LSTPDTVDQN IQPDFSKYGL VVMLSMYGST ARMITSKWLR
810 820 830 840 850
KHGIATVEAS DWNELTQIIR DLLETGSRDN SFDSQHNISD PLRAELSNIV
860 870 880 890 900
EIKNPVFVIV VDIGVLDLTT NIWKEQLNYL DRFSNKAKFA WLLKHDTSNT
910 920 930 940 950
VKTELRRKGH VMMVNKPLYK AKMIQILEAV IKNRKRGLCN DLRNRGNGSD
960 970 980 990 1000
ESHDCLEIDP TQFDTCSSDD SSETSGEKQV DKSVKPSTLH SPVLKNYLID
1010 1020 1030 1040 1050
ATTSNDDSTS ASMTQKNPEE EDWKDRLYSG IALDGKNQKS LEGIRILLAE
1060 1070 1080 1090 1100
DTPVLQRVAT IMLEKMGATV TAVWDGQQAV DSLNYKSINA QAPTEEHKSF
1110 1120 1130 1140 1150
EEETANKVTT RETSLRNSSP YDLILMDCQM PKMDGYEATK AIRRAEIGTE
1160 1170 1180 1190 1200
LHIPIVALTA HAMSSDEAKC LEVGMDAYLT KPIDRKLMVS TILSLTKPSA

FQTSLSA
Length:1,207
Mass (Da):135,449
Last modified:May 3, 2011 - v2
Checksum:i76892FC99352E149
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1046 – 10461I → F in BAA32085 (PubMed:10488240).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010914 mRNA. Translation: BAA32085.1.
CP002685 Genomic DNA. Translation: AEC06691.1.
PIRiT08856.
T52459.
RefSeqiNP_565424.1. NM_127335.1.
UniGeneiAt.5429.

Genome annotation databases

EnsemblPlantsiAT2G17820.1; AT2G17820.1; AT2G17820.
GeneIDi816291.
GrameneiAT2G17820.1; AT2G17820.1; AT2G17820.
KEGGiath:AT2G17820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010914 mRNA. Translation: BAA32085.1.
CP002685 Genomic DNA. Translation: AEC06691.1.
PIRiT08856.
T52459.
RefSeqiNP_565424.1. NM_127335.1.
UniGeneiAt.5429.

3D structure databases

ProteinModelPortaliQ9SXL4.
SMRiQ9SXL4. Positions 499-753, 1044-1195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1648. 2 interactions.
IntActiQ9SXL4. 1 interaction.
STRINGi3702.AT2G17820.1.

Proteomic databases

PaxDbiQ9SXL4.
PRIDEiQ9SXL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G17820.1; AT2G17820.1; AT2G17820.
GeneIDi816291.
GrameneiAT2G17820.1; AT2G17820.1; AT2G17820.
KEGGiath:AT2G17820.

Organism-specific databases

TAIRiAT2G17820.

Phylogenomic databases

eggNOGiENOG410IRDA. Eukaryota.
ENOG410XT1K. LUCA.
HOGENOMiHOG000029544.
InParanoidiQ9SXL4.
OMAiWHFTRIY.

Enzyme and pathway databases

BioCyciARA:AT2G17820-MONOMER.

Miscellaneous databases

PROiQ9SXL4.

Gene expression databases

GenevisibleiQ9SXL4. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 2 hits.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A transmembrane hybrid-type histidine kinase in Arabidopsis functions as an osmosensor."
    Urao T., Yakubov B., Satoh R., Yamaguchi-Shinozaki K., Seki M., Hirayama T., Shinozaki K.
    Plant Cell 11:1743-1754(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF HIS-508 AND ASP-1075.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system."
    Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K.
    FEBS Lett. 478:227-232(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHP2, MUTAGENESIS OF ASP-1075.
  5. "His-Asp phosphorelay signal transduction in higher plants: receptors and response regulators for cytokinin signaling in Arabidopsis thaliana."
    Oka A., Sakai H., Iwakoshi S.
    Genes Genet. Syst. 77:383-391(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Two-component signal transduction pathways in Arabidopsis."
    Hwang I., Chen H.-C., Sheen J.
    Plant Physiol. 129:500-515(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis."
    Tran L.S., Urao T., Qin F., Maruyama K., Kakimoto T., Shinozaki K., Yamaguchi-Shinozaki K.
    Proc. Natl. Acad. Sci. U.S.A. 104:20623-20628(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
  8. "Analysis of the Arabidopsis histidine kinase ATHK1 reveals a connection between vegetative osmotic stress sensing and seed maturation."
    Wohlbach D.J., Quirino B.F., Sussman M.R.
    Plant Cell 20:1101-1117(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAHK1_ARATH
AccessioniPrimary (citable) accession number: Q9SXL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 3, 2011
Last modified: April 13, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.