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Protein

ATP-dependent Clp protease proteolytic subunit 3, chloroplastic

Gene

CLPP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). In the absence of CLPP3, modified ClpPR core(s) could be formed, albeit at strongly reduced levels (PubMed:23548781).By similarity1 Publication

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164NucleophileBy similarity1
Active sitei189By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS14.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit 3, chloroplastic1 Publication (EC:3.4.21.92)
Alternative name(s):
Endopeptidase ClpP31 Publication
Short name:
nClpP3
nClpP4
Gene namesi
Name:CLPP31 Publication
Synonyms:CLP, NCLPP3, NCLPP4
Ordered Locus Names:At1g66670Imported
ORF Names:F4N21.19Imported, T12I7.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G66670.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplastic endopeptidase Clp complex Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • plastid stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Delayed embryo development and seedling lethality. Can be rescued by adding sugars to the growth medium.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastCombined sourcesAdd BLAST70
ChainiPRO_000030897871 – 309ATP-dependent Clp protease proteolytic subunit 3, chloroplasticAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71N-acetylvalineCombined sources1
Modified residuei194PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated in vitro by CHIP.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9SXJ6.
PRIDEiQ9SXJ6.

PTM databases

iPTMnetiQ9SXJ6.

Expressioni

Tissue specificityi

Mostly expressed in leaves. Also detected in stems, and to a lower extent, in roots (at protein level).1 Publication

Inductioni

Repressed in darkness. Accumulates during leaf senescence. Induced during cold acclimation (at protein level).2 Publications

Gene expression databases

GenevisibleiQ9SXJ6. AT.

Interactioni

Subunit structurei

Component of the chloroplastic Clp protease core complex which consist of at least 16 proteins: CLPP4 (3 copies), CLPP5 (3 copies), CLPR4 (2 copies), ClpP1 (1 copy), CLPP6 (1 copy), CLPR2 (1 copy), CLPT1 (1 copy), CLPT2 (1 copy) and 3 copies of CLPP3 and/or CLPR1 and/or CLPR3 (PubMed:11278690, PubMed:14593120, PubMed:16766689). The core complex is organized in two heptameric rings, one containing CLPP3,4,5,6 in a 1:2:3:1 ratio and the other CLPP1 and CLPR1,2,3,4 in a 3:1:1:1:1 ratio (PubMed:21712416). Interacts with CHIP (PubMed:26085677).6 Publications

Protein-protein interaction databases

BioGridi28206. 4 interactors.
IntActiQ9SXJ6. 1 interactor.
STRINGi3702.AT1G66670.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R90model-A1-309[»]
ProteinModelPortaliQ9SXJ6.
SMRiQ9SXJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
HOGENOMiHOG000285833.
InParanoidiQ9SXJ6.
KOiK01358.
OMAiDAMKQCK.
OrthoDBiEOG09360JWQ.
PhylomeDBiQ9SXJ6.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SXJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMSLRLASS STSNPICLLN PGKNLNFPIR NHRIPKTSKP FCVRSSMSLS
60 70 80 90 100
KPPRQTLSSN WDVSSFSIDS VAQSPSRLPS FEELDTTNML LRQRIVFLGS
110 120 130 140 150
QVDDMTADLV ISQLLLLDAE DSERDITLFI NSPGGSITAG MGIYDAMKQC
160 170 180 190 200
KADVSTVCLG LAASMGAFLL ASGSKGKRYC MPNSKVMIHQ PLGTAGGKAT
210 220 230 240 250
EMSIRIREMM YHKIKLNKIF SRITGKPESE IESDTDRDNF LNPWEAKEYG
260 270 280 290 300
LIDAVIDDGK PGLIAPIGDG TPPPKTKVWD LWKVEGTKKD NTNLPSERSM

TQNGYAAIE
Length:309
Mass (Da):33,925
Last modified:May 1, 2000 - v1
Checksum:i0D2D0D3D1118AA0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 4MEMS → NSARG in AAC35489 (Ref. 1) Curated4
Sequence conflicti289K → E in AAK48955 (PubMed:14593172).Curated1
Sequence conflicti289K → E in AAL66941 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032123 mRNA. Translation: AAC35489.1.
AB022328 mRNA. Translation: BAA82067.1.
AC013288 Genomic DNA. Translation: AAG60075.1.
AC079285 Genomic DNA. Translation: AAG51173.1.
CP002684 Genomic DNA. Translation: AEE34543.1.
AF370528 mRNA. Translation: AAK48955.1.
AY072526 mRNA. Translation: AAL66941.1.
AY087349 mRNA. Translation: AAM64899.1.
PIRiT52041.
T52453.
RefSeqiNP_564880.1. NM_105338.4.
UniGeneiAt.21167.

Genome annotation databases

EnsemblPlantsiAT1G66670.1; AT1G66670.1; AT1G66670.
GeneIDi842985.
GrameneiAT1G66670.1; AT1G66670.1; AT1G66670.
KEGGiath:AT1G66670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032123 mRNA. Translation: AAC35489.1.
AB022328 mRNA. Translation: BAA82067.1.
AC013288 Genomic DNA. Translation: AAG60075.1.
AC079285 Genomic DNA. Translation: AAG51173.1.
CP002684 Genomic DNA. Translation: AEE34543.1.
AF370528 mRNA. Translation: AAK48955.1.
AY072526 mRNA. Translation: AAL66941.1.
AY087349 mRNA. Translation: AAM64899.1.
PIRiT52041.
T52453.
RefSeqiNP_564880.1. NM_105338.4.
UniGeneiAt.21167.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R90model-A1-309[»]
ProteinModelPortaliQ9SXJ6.
SMRiQ9SXJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28206. 4 interactors.
IntActiQ9SXJ6. 1 interactor.
STRINGi3702.AT1G66670.1.

Protein family/group databases

MEROPSiS14.A03.

PTM databases

iPTMnetiQ9SXJ6.

Proteomic databases

PaxDbiQ9SXJ6.
PRIDEiQ9SXJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66670.1; AT1G66670.1; AT1G66670.
GeneIDi842985.
GrameneiAT1G66670.1; AT1G66670.1; AT1G66670.
KEGGiath:AT1G66670.

Organism-specific databases

TAIRiAT1G66670.

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
HOGENOMiHOG000285833.
InParanoidiQ9SXJ6.
KOiK01358.
OMAiDAMKQCK.
OrthoDBiEOG09360JWQ.
PhylomeDBiQ9SXJ6.

Miscellaneous databases

PROiQ9SXJ6.

Gene expression databases

GenevisibleiQ9SXJ6. AT.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPP3_ARATH
AccessioniPrimary (citable) accession number: Q9SXJ6
Secondary accession number(s): O82421, Q94JY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.