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Q9SXC9 (MNS5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha-mannosidase I MNS5
EC=3.2.1.-
Gene names
Name:MNS5
Ordered Locus Names:At1g27520
ORF Names:T17H3.2
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in N-glycan processing By similarity. Ref.4

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Probable alpha-mannosidase I MNS5
PRO_0000397937

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 2617Helical; Signal-anchor for type II membrane protein; Potential
Topological domain27 – 574548Lumenal Potential

Sites

Active site1341Proton donor By similarity
Active site2741 By similarity
Active site3881 By similarity

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation2011N-linked (GlcNAc...) Potential
Glycosylation3491N-linked (GlcNAc...) Potential
Glycosylation4941N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9SXC9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 4ACC456DE487EA93

FASTA57465,708
        10         20         30         40         50         60 
MSCPIHPRRL FLCLLISLTF FVVDPSSQHI EVKKKQMREK VREMFYHAYD NYMTYAFPHD 

        70         80         90        100        110        120 
ELKPLTKSFT DSLSELGNLK LEHLPTDYNG SAVTLVESLS SLAILGNSTE FEKGVLWLSE 

       130        140        150        160        170        180 
NLTFDIDARV NLFECNIRVL GGLISAHLLA IDPNNRLIQG SYNNQLLRLA EDLGKRFLPA 

       190        200        210        220        230        240 
FETPTGLPYA WINLKNGVME NETTETSTSG CGSLVLEMGA LSRLTGDPRF ESAALRALRQ 

       250        260        270        280        290        300 
LWRMRSSLDL LGTTLDVVTG EWIEYSSSIG AGVDSFYEYL LKAYILFGKE DYWRMFHSAY 

       310        320        330        340        350        360 
LASQKYFRHG PWYHEANMWS GKPTYWQLTS LQAFWPGLQV LVGDIAAANS SHREFFHVWE 

       370        380        390        400        410        420 
KFGVLPERYL LDHQIIHPTM KYYPLRPELA ESTFYLYQAT KDPWYLDVGE SMVKSLNLYT 

       430        440        450        460        470        480 
KVPGGFASVR DVTTMQLEDH QHSFFLAETC KYLYLLFDDS FVAKRNYIFT TEGHPIQVVS 

       490        500        510        520        530        540 
SWHEKLPETY FSGNWTLSKS GAWESRASAL SLQVCPLISL NSRHPEQQRE SACHVLDEQI 

       550        560        570 
NHKCWSNKEC GVDATTCRLR TCSGVGYCGL WNPL

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
Plant Cell 21:3850-3867(2009) [PubMed: 20023195] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC005916 Genomic DNA. Translation: AAD45990.1.
CP002684 Genomic DNA. Translation: AEE30841.1.
AY056382 mRNA. Translation: AAL08238.1.
IPIIPI00525476.
PIRC86400.
RefSeqNP_564288.1. NM_102516.3.
UniGeneAt.24198.

3D structure databases

HSSPHSSP built from PDB template 1FO3 based on UniProtKB Q9UKM7.
ProteinModelPortalQ9SXC9.
SMRQ9SXC9. Positions 28-480.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SXC9.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

Proteomic databases

PRIDEQ9SXC9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G27520.1; AT1G27520.1; AT1G27520.
GeneID839643.
GenomeReviewsGene locus AT1G27520 in contig CT485782_GR.
KEGGath:AT1G27520.
NMPDRfig|3702.1.peg.3080.

Organism-specific databases

TAIRAt1g27520.

Phylogenomic databases

eggNOGKOG2429.
GeneTreeEPGT00050000018377.
HOGENOMHBG562188.
InParanoidQ9SXC9.
OMALFECNIR.
PhylomeDBQ9SXC9.
ProtClustDBCLSN2679415.

Gene expression databases

GenevestigatorQ9SXC9.

Family and domain databases

InterProIPR001382. Glyco_hydro_47.
[Graphical view]
Gene3DG3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
KOK10084.
PANTHERPTHR11742. Glyco_hydro_47. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. Glyco_hydro_47. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNS5_ARATH
AccessionPrimary (citable) accession number: Q9SXC9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 2000
Last modified: November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents