Putative phospholipid-transporting ATPase 9 - Arabidopsis thaliana (Mouse-ear cress)

Names and origin

Protein names

Recommended name:
    Putative phospholipid-transporting ATPase 9
      Short name=AtALA9
    EC=3.6.3.1
Alternative name(s):
    Aminophospholipid flippase 9

Gene names

Name:	ALA9
Ordered Locus Names:	At1g68710
ORF Names:	F24J5.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H₂O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1200

1200

Putative phospholipid-transporting ATPase 9

PRO_0000046393

Regions

Topological domain

1 – 75

75

Cytoplasmic Potential

Transmembrane

76 – 97

22

Potential

Topological domain

98 – 101

4

Extracellular Potential

Transmembrane

102 – 124

23

Potential

Topological domain

125 – 306

182

Cytoplasmic Potential

Transmembrane

307 – 328

22

Potential

Topological domain

329 – 364

36

Extracellular Potential

Transmembrane

365 – 382

18

Potential

Topological domain

383 – 925

543

Cytoplasmic Potential

Transmembrane

926 – 945

20

Potential

Topological domain

946 – 959

14

Extracellular Potential

Transmembrane

960 – 979

20

Potential

Topological domain

980 – 1009

30

Cytoplasmic Potential

Transmembrane

1010 – 1032

23

Potential

Topological domain

1033 – 1045

13

Extracellular Potential

Transmembrane

1046 – 1068

23

Potential

Topological domain

1069 – 1074

6

Cytoplasmic Potential

Transmembrane

1075 – 1095

21

Potential

Topological domain

1096 – 1112

17

Extracellular Potential

Transmembrane

1113 – 1137

25

Potential

Topological domain

1138 – 1200

63

Cytoplasmic Potential

Sites

Active site

430

1

4-aspartylphosphate intermediate By similarity

Metal binding

870

1

Magnesium By similarity

Metal binding

874

1

Magnesium By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9SX33-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: E8CAB216E2D2B261
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FASTA

1,200

136,045

        10         20         30         40         50         60 
MVGGGTKRRR RRLQLSKLYT LTCAQACFKQ DHSQIGGPGF SRVVYCNEPD SPEADSRNYS 

        70         80         90        100        110        120 
DNYVRTTKYT LATFLPKSLF EQFRRVANFY FLVTGVLAFT PLAPYTASSA IVPLLFVIGA 

       130        140        150        160        170        180 
TMVKEGVEDW RRQKQDNEVN NRKVKVHRGD GSFDAKEWKT LSIGDIVKVE KNEFFPADLV 

       190        200        210        220        230        240 
LLSSSYEDAI CYVETMNLDG ETNLKVKQGL EVTSSLRDEF NFKGFEAFVK CEDPNANLYS 

       250        260        270        280        290        300 
FVGTMELKGA KYPLSPQQLL LRDSKLRNTD FIFGAVIFTG HDTKVIQNST DPPSKRSMIE 

       310        320        330        340        350        360 
KKMDKIIYLM FFMVITMAFI GSVIFGVTTR DDLKDGVMKR WYLRPDSSSI FFDPKRAPVA 

       370        380        390        400        410        420 
AIYHFLTAVM LYSYFIPISL YVSIEIVKVL QSIFINQDIH MYYEEADKPA RARTSNLNEE 

       430        440        450        460        470        480 
LGQVDTILSD KTGTLTCNSM EFIKCSVAGT AYGRGVTEVE MAMGRRKGGP LVFQSDENDI 

       490        500        510        520        530        540 
DMEYSKEAIT EESTVKGFNF RDERIMNGNW VTETHADVIQ KFFRLLAVCH TVIPEVDEDT 

       550        560        570        580        590        600 
EKISYEAESP DEAAFVIAAR ELGFEFFNRT QTTISVRELD LVSGKRVERL YKVLNVLEFN 

       610        620        630        640        650        660 
STRKRMSVIV QEEDGKLLLL CKGADNVMFE RLSKNGREFE EETRDHVNEY ADAGLRTLIL 

       670        680        690        700        710        720 
AYRELDEKEY KVFNERISEA KSSVSADRES LIEEVTEKIE KDLILLGATA VEDKLQNGVP 

       730        740        750        760        770        780 
DCIDKLAQAG IKIWVLTGDK METAINIGFA CSLLRQDMKQ IIINLETPEI QSLEKTGEKD 

       790        800        810        820        830        840 
VIAKASKENV LSQIINGKTQ LKYSGGNAFA LIIDGKSLAY ALDDDIKHIF LELAVSCASV 

       850        860        870        880        890        900 
ICCRSSPKQK ALVTRLVKSG NGKTTLAIGD GANDVGMLQE ADIGVGISGV EGMQAVMSSD 

       910        920        930        940        950        960 
IAIAQFRYLE RLLLVHGHWC YRRISTMICY FFYKNITFGF TLFLYETYTT FSSTPAYNDW 

       970        980        990       1000       1010       1020 
FLSLYNVFFS SLPVIALGVF DQDVSARYCL KFPLLYQEGV QNVLFSWRRI LGWMFNGFYS 

      1030       1040       1050       1060       1070       1080 
AVIIFFLCKS SLQSQAFNHD GKTPGREILG GTMYTCIVWV VNLQMALAIS YFTLIQHIVI 

      1090       1100       1110       1120       1130       1140 
WSSIVVWYFF ITVYGELPSR ISTGAYKVFV EALAPSLSYW LITLFVVVAT LMPYFIYSAL 

      1150       1160       1170       1180       1190       1200 
QMSFFPMYHG MIQWLRYEGQ CNDPEYCDIV RQRSIRPTTV GFTARLEAKK RSVRISEPAS

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References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.

Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

+

Additional computationally mapped references.

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Cross-references

Sequence databases
	AC008075 Genomic DNA. Translation: AAD49973.1.
IPI	IPI00535521.
PIR	F96711.
RefSeq	NP_177038.1.
UniGene	At.50013
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9SX33.
Genome annotation databases
GeneID	843201.
GenomeReviews	Gene locus AT1G68710 in contig CT485782_GR.
KEGG	ath:AT1G68710.
NMPDR	fig\|3702.1.peg.6326.
Organism-specific databases
TAIR	At1g68710.
Phylogenomic databases
OMA	FTLIQHI.
Enzyme and pathway databases
BRENDA	3.6.3.1. 302.
Gene expression databases
ArrayExpress	Q9SX33.
Genevestigator	Q9SX33.
GermOnline	AT1G68710. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR005834. Dehalogen-like_hydro. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ALA9_ARATH

Accession

Primary (citable) accession number: Q9SX33

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents